Ryota Mori, Masami Abe, Yuma Saimoto, Saki Shinto, Sara Jodai, Manami Tomomatsu, Kaho Tazoe, Minato Ishida, Masataka Enoki, Nao Kato, Tomohiro Yamashita, Yuki Itabashi, Ikuo Nakanishi, Kei Ohkubo, Sachiko Kaidzu, Masaki Tanito, Yuta Matsuoka, Kazushi Morimoto, Ken-Ichi Yamada
Recent studies have highlighted the indispensable role of oxidized lipids in inflammatory responses, cell death, and disease pathogenesis. Consequently, inhibitors targeting oxidized lipids, particularly lipid-derived radicals critical in lipid peroxidation, which are known as radical-trapping antioxidants (RTAs), have been actively pursued. We focused our investigation on nitroxide compounds that have rapid second-order reaction rate constants for reaction with lipid-derived radicals. A novel screening system was developed by employing competitive reactions between library compounds and a newly developed profluorescence nitroxide probe with lipid-derived radicals to identify RTA compounds...
May 8, 2024: Redox Biology