keyword
https://read.qxmd.com/read/38523597/circitga7-regulates-malignant-phenotypes-in-bladder-cancer-cells-via-targeting-mir-330-3p-klf10-axis
#21
JOURNAL ARTICLE
Xian-Xu Yang, Chao Wang
Bladder cancer (BCa) is one of the common malignancies. Circular RNAs (circRNAs) play regulatory roles in cancer progression. CircITGA7 is a circRNA generated from several exons of ITGA7. The potential role of circITGA7 in BCa remains unknown and needs to be explored. Quantitative real time polymerase chain reaction (qRT-PCR) was used to assess circITGA7 and miR-330-3p expression in BCa tissues and cell lines. Kaplan-Meier analysis was used to evaluate the overall survival of these BCa patients. The biological function of circITGA7 was examined by overexpression of circITGA7 using CCK-8, EdU, wound-healing, and Transwell assays...
March 25, 2024: Kaohsiung Journal of Medical Sciences
https://read.qxmd.com/read/38522124/amplification-of-different-satellite-dnas-in-prostate-cancer
#22
JOURNAL ARTICLE
Nurul Aida Ariffen, Antonio Augusto Ornellas, Gilda Alves, Ahmad Moay Shana'ah, Samiha Sharma, Stefanie Kankel, Elena Jamali, Bernhard Theis, Thomas Liehr
In various solid tumors and corresponding cell lines, prior research has identified acquired copy number variations (CNVs) encompassing centromeric satellite-DNA sequences. This observation emerged from the application of centromeric probes (satellite-DNA) as controls in molecular cytogenetic investigations and diagnostics, although these accounts were largely anecdotal. In this study, we conducted a systematic screening for satellite-DNA sequence amplification in 31 prostate cancer (PCa) samples, a prevalent malignancy in men characterized by discernible molecular cytogenetic aberrations...
March 21, 2024: Pathology, Research and Practice
https://read.qxmd.com/read/38522075/high-grade-b-cell-lymphoma-with-a-quadruple-hit-genetic-profile-including-concurrent-myc-bcl2-bcl6-and-ccnd1-gene-rearrangements
#23
JOURNAL ARTICLE
Marie-France Gagnon, Reid G Meyer, Eric J Weaver, Adam J Wood, Dudley A Dupuy, Sudeep J Menachery, Min Shi, Linda B Baughn, Rhett P Ketterling, Jess F Peterson
Several reports of concurrent MYC, BCL2, BCL6, and CCND1 rearrangements in high-grade B-cell lymphoma (HGBL) have been recently described. Herein, we aimed to delineate the scope of this entity through a review of HGBL with a "quadruple-hit" genetic profile identified at our institution. We performed a retrospective review (2015-2023) at our institution of B-cell lymphoma (BCL) cases that were evaluated with concurrent MYC, BCL2, and BCL6 break-apart and IGH::MYC and IGH::CCND1 dual-color dual-fusion fluorescence in situ hybridization studies...
March 24, 2024: Laboratory Medicine
https://read.qxmd.com/read/38521178/eldecalcitol-protected-osteocytes-against-ferroptosis-of-d-gal-induced-senescent-mlo-y4-cells-and-ovariectomized-mice
#24
JOURNAL ARTICLE
Yong-Fang Fu, Yi-Xun Guo, Shi-Hui Xia, Ting-Ting Zhou, Yun-Chao Zhao, Zhen-Hua Jia, Yan Zhang
BACKGROUND: Active vitamin D analog eldecalcitol is clinically applied in treatment of postmenopausal osteoporosis. This study aims to determine the role of eldecalcitol in the protection of osteocytes from senescence and the associated ferroptosis. METHODS: The MLO-Y4 osteocytes were exposed to D-gal inducing senescence. The ovariectomized (OVX) mice treated with D-gal using as an aging inducer were intraperitoneally injected with eldecalcitol. The multiplexed confocal imaging, fluorescence in situ hybridization and transmission electron microscopy were applied in assessing osteocytic properties...
March 21, 2024: Experimental Gerontology
https://read.qxmd.com/read/38521112/identify-ctbp1-dt-as-an-immunological-biomarker-that-promotes-lipid-synthesis-and-apoptosis-resistance-in-kirc
#25
JOURNAL ARTICLE
Haolin Li, Mintian Fei, Yi Zhang, Qili Xu, Rui Feng, Jing Cao, Yan Qu, Haibing Xiao
Recently, mounting evidence has highlighted the essential function of the C-terminal binding protein-1 divergent transcript (CTBP1-DT) in malignancies. However, its role in kidney renal clear cell carcinoma (KIRC) remains largely unknown. Our study aimed to identify the potential function of CTBP1-DT in KIRC. RT-qPCR, Kaplan-Meier survival analysis, Cox regression analysis, and nomogram analysis were utilized to determine the expression and effects of CTBP1-DT on survival. The subcellular localization of CTBP1-DT was determined using RNA fluorescence in situ hybridization (FISH)...
March 21, 2024: Gene
https://read.qxmd.com/read/38520054/rnascope-a-novel-method-for-the-detection-of-heterobilharzia-americana-ova-in-canine-liver
#26
JOURNAL ARTICLE
Priscilla A Devora, Andrea N Johnston
Canine schistosomiasis caused by Heterobilharzia americana can lead to severe morbidity and eventual mortality, in part due to the deposition of fluke ova in the liver and gastrointestinal tract, which promotes an influx of peri-ova inflammatory cells. Although fluke eggs can be identified in H&E-stained histologic sections, cases exist in which only fragments of the ova persist, or the egg is obscured by inflammatory infiltrates, which can confound definitive histologic diagnosis. Unfortunately, antibodies specific to Heterobilharzia are not commercially available for immunohistochemical labeling...
March 22, 2024: Journal of Veterinary Diagnostic Investigation
https://read.qxmd.com/read/38519987/depletion-of-lamins-b1-and-b2-promotes-chromatin-mobility-and-induces-differential-gene-expression-by-a-mesoscale-motion-dependent-mechanism
#27
JOURNAL ARTICLE
Emily M Pujadas Liwag, Xiaolong Wei, Nicolas Acosta, Lucas M Carter, Jiekun Yang, Luay M Almassalha, Surbhi Jain, Ali Daneshkhah, Suhas S P Rao, Fidan Seker-Polat, Kyle L MacQuarrie, Joe Ibarra, Vasundhara Agrawal, Erez Lieberman Aiden, Masato T Kanemaki, Vadim Backman, Mazhar Adli
BACKGROUND: B-type lamins are critical nuclear envelope proteins that interact with the three-dimensional genomic architecture. However, identifying the direct roles of B-lamins on dynamic genome organization has been challenging as their joint depletion severely impacts cell viability. To overcome this, we engineered mammalian cells to rapidly and completely degrade endogenous B-type lamins using Auxin-inducible degron technology. RESULTS: Using live-cell Dual Partial Wave Spectroscopic (Dual-PWS) microscopy, Stochastic Optical Reconstruction Microscopy (STORM), in situ Hi-C, CRISPR-Sirius, and fluorescence in situ hybridization (FISH), we demonstrate that lamin B1 and lamin B2 are critical structural components of the nuclear periphery that create a repressive compartment for peripheral-associated genes...
March 22, 2024: Genome Biology
https://read.qxmd.com/read/38519939/acylcarnitines-promote-gallbladder-cancer-metastasis-through-lncbcl2l11-thoc5-jnk-axis
#28
JOURNAL ARTICLE
Yang Yang, Huaifeng Li, Ke Liu, Lu Zou, Shanshan Xiang, Yajun Geng, Xuechuan Li, Shimei Qiu, Jiahua Yang, Xuya Cui, Lin Li, Yang Li, Weijian Li, Siyuan Yan, Liguo Liu, Xiangsong Wu, Fatao Liu, Wenguang Wu, Shili Chen, Yingbin Liu
BACKGROUND: The progression of gallbladder cancer (GBC) is accompanied by abnormal fatty acid β-oxidation (FAO) metabolism. Different types of lipids perform various biological functions. This study aimed to determine the role of acyl carnitines in the molecular mechanisms of GBC progression. METHODS: Distribution of lipids in GBC was described by LC-MS-based lipidomics. Cellular localization, expression level and full-length of lncBCL2L11 were detected using fluorescence in situ hybridization (FISH) assays, subcellular fractionation assay and 5' and 3' rapid amplification of the cDNA ends (RACE), respectively...
March 22, 2024: Journal of Translational Medicine
https://read.qxmd.com/read/38514361/reliably-making-the-primary-diagnosis-of-mesothelioma-utilizing-serous-fluid-cytology-specimens-an-institutional-experience
#29
JOURNAL ARTICLE
Terri E Jones, Daniel L Geisler, Swikrity Upadhyay Baskota, N Paul Ohori, Jacqueline Cuda, Samer N Khader
INTRODUCTION: The diagnosis of mesothelioma has historically been challenging, especially on serous fluid cytology (SFC). Distinguishing between reactive and neoplastic mesothelial cells can be difficult on cytomorphology alone. However, additional ancillary tests, such as BRCA1 associated protein-1 immunohistochemistry and fluorescence in situ hybridization for cyclin-dependent kinase inhibitor 2A deletion, can provide a sensitive and highly specific method of proving malignancy. MATERIALS AND METHODS: SFC specimens diagnosed as mesothelioma, suspicious for mesothelioma (SM), and atypical mesothelial cells (AMCs) since 2012 were identified by querying the laboratory information system...
March 2, 2024: Journal of the American Society of Cytopathology
https://read.qxmd.com/read/38511052/abnormal-expression-of-prkag2-as1-in-endothelial-cells-induced-inflammation-and-apoptosis-by-reducing-prkag2-expression
#30
JOURNAL ARTICLE
Xiao-Wei Song, Wen-Xia He, Ting Su, Chang-Jin Li, Li-Li Jiang, Song-Qun Huang, Song-Hua Li, Zhi-Fu Guo, Bi-Li Zhang
PRKAG2 is required for the maintenance of cellular energy balance. PRKAG2-AS1, a long non-coding RNA (lncRNA), was found within the promoter region of PRKAG2. Despite the extensive expression of PRKAG2-AS1 in endothelial cells, the precise function and mechanism of this gene in endothelial cells have yet to be elucidated. The localization of PRKAG2-AS1 was predominantly observed in the nucleus, as revealed using nuclear and cytoplasmic fractionation and fluorescence in situ hybridization. The manipulation of PRKAG2-AS1 by knockdown and overexpression within the nucleus significantly altered PRKAG2 expression in a cis -regulatory manner...
June 2024: Non-Coding RNA Research
https://read.qxmd.com/read/38511042/atypical-co-amplification-with-co-localization-of-her2-gene-in-breast-cancer-combined-ihc-fish-approach-as-per-asco-cap-2018-guidelines-for-targeted-therapy-eligibility
#31
JOURNAL ARTICLE
Ushang Kate, Anurita Pais, Neelam Kamble, Sandhya Kandoor, Kunal Sharma
Breast cancer patients with HER2 gene amplification as assessed by FISH are eligible for HER2-targeted therapy. However, in a small subset of patients, unusual FISH pattern of co-localization and co-amplification can pose challenges in interpretation of the HER2 status and hence to assess the HER2 status accurately; our aim was to report their incidence and analyze them based on latest ASCO/CAP 2018 guidelines. We present seven cases with HER2/CEP17 co-amplification and co-localization from a total 4040 cases referred during the year 2017 to 2021 at Mumbai Reference Laboratory, SRL Diagnostics...
March 2024: Indian Journal of Surgical Oncology
https://read.qxmd.com/read/38510893/hepatic-angiomyolipoma-with-predominant-lipomatous-component-a-rare-entity
#32
Jaweria Pervaiz, Samina Zaman, Sohaib Khalid, Zubaria Rafique, Rida Noor
Hepatic angiomyolipoma (HAML) is a rare benign mesenchymal tumor with varying amounts of mature adipose tissue, smooth muscle cells, and thick-walled blood vessels. We present a rare case of hepatic angiomyolipoma (AML) with predominant lipomatous components. A 42-year-old female presented to the hospital with pain in the right lumbar region. On imaging, there was a large fat-predominant mass attached to the surface of the liver extending down to the lumbar region. On small biopsy, it was reported as a well-differentiated adipocytic neoplasm, and fluorescence in situ hybridization (FISH) studies performed for MDM2 were negative...
February 2024: Curēus
https://read.qxmd.com/read/38510678/bone-metastasis-in-non-small-cell-lung-cancer-genomic-characterization-and-exploration-of-potential-targets
#33
JOURNAL ARTICLE
Jiali Gong, Shumin Hu, Qianyun Shan, Jing Qin, Na Han, Fajun Xie, Hongyang Lu
BACKGROUND: Bone metastasis (BM) seriously affects the quality of life and reduces the survival time of patients with non-small-cell lung cancer (NSCLC). The genomic characteristics and potential targets of BMs are yet to be fully explored. OBJECTIVE: To explore the genetic characteristics and potential targets of BM in NSCLC. DESIGN: In all, 83 patients with NSCLC were retrospectively selected in this study. Genomic characterization of BMs was explored with the analysis of NGS results from primary tumors and BMs in 6 patients, then combined with NGS results of lung tumors in 16 patients with initial recurrence in bone to analyze mutations potentially associated with BMs, and finally, the correlation was further validated in 61 postoperative patients...
2024: Therapeutic Advances in Medical Oncology
https://read.qxmd.com/read/38509869/testicular-primary-well-differentiated-neuroendocrine-tumor-clinicopathologic-immunohistochemical-and-molecular-characterization-of-two-patients
#34
JOURNAL ARTICLE
Liwei Jia, Bo Zhang, Daniel Shen, Prasad R Koduru
Well-differentiated neuroendocrine tumor rarely occurs as a testicular primary tumor, accounting for less than 1% of all testicular cancers, and is rarely reported with sufficient molecular profiles. After searching our departmental database (2003-2023), two testicular primary well-differentiated neuroendocrine tumors were identified in a 35-year-old man and a 23-year-old man, respectively, both of whom had normal serum level of tumor markers. Both tumors grossly exhibited solid, yellow-tan, and homogeneous appearance and histologically displayed a mixture of growth patterns, including organoid, tubular, cribriform, nests, cords, and single cells, were composed of eosinophilic tumor cells with salt-and-pepper chromatin and indistinct cell borders...
March 21, 2024: International Journal of Surgical Pathology
https://read.qxmd.com/read/38507464/egfr-amplification-and-egfrviii-predict-and-participate-in-tat-cx43266-283-antitumor-response-in-preclinical-glioblastoma-models
#35
JOURNAL ARTICLE
Andrea Álvarez-Vázquez, Laura San-Segundo, Pilar Cerveró-García, Raquel Flores-Hernández, Claudia Ollauri-Ibáñez, Berta Segura-Collar, C G Hubert, Gillian Morrison, Steven M Pollard, Justin D Lathia, Pilar Sánchez-Gómez, Arantxa Tabernero
BACKGROUND: Glioblastoma (GBM) commonly displays epidermal growth factor receptor (EGFR) alterations (mainly amplification and EGFRvIII) and TAT-Cx43266-283 is a Src-inhibitory peptide with antitumor properties in preclinical GBM models. Given the link between EGFR and Src, the aim of this study was to explore the role of EGFR in the antitumor effects of TAT-Cx43266-283. METHODS: The effect of TAT-Cx43266-283, temozolomide (TMZ) and erlotinib (EGFR inhibitor) was studied in patient-derived GBM stem cells (GSCs) and murine neural stem cells (NSCs) with and without EGFR alterations, in vitro and in vivo...
March 20, 2024: Neuro-oncology
https://read.qxmd.com/read/38506240/integration-of-hepatitis-b-virus-into-patients-sperm-genome-and-its-clinical-risks
#36
JOURNAL ARTICLE
Ting-Ting Han, Ji-Hua Huang, Ling-Xiao Li, Xue Liao, Xiang-Qian Meng, Zi-Na Wen, Qian Sun, Jian Ma, Tian-Hua Huang
BACKGROUND: Like the coronavirus disease 2019, the hepatitis B virus is also wreaking havoc worldwide, which has infected over 2 billion people globally. Using an experimental animal model, our previous research observed that the hepatitis B virus genes integrated into human spermatozoa can replicate and express after being transmitted to embryos. However, as of now, this phenomenon has not been confirmed in clinical data from patients. OBJECTIVES: To explore the integration of the hepatitis B virus into patients' sperm genome and its potential clinical risks...
March 20, 2024: Andrology
https://read.qxmd.com/read/38504661/synchronous-low-grade-central-osteosarcoma-and-ewing-sarcoma-a-rare-case-report
#37
JOURNAL ARTICLE
Daniel Christensen, Jeffrey A Belair, Atrayee BasuMallick, Scot A Brown, Michael Klein, Wei Jiang
A 23-year-old female patient presented with radicular back pain, perineal numbness, and urinary retention. The patient was diagnosed with cauda equina syndrome and magnetic resonance imaging (MRI) of the spine revealed an enhancing osseous lumbar lesion causing severe central stenosis. A core needle biopsy of the lumbar spine showed microscopic features compatible with a small round blue cell tumor. CD99 and FLI1 were positive in the tumor cells. Next-generation sequencing demonstrated a EWSR1::FLI1 fusion...
March 20, 2024: International Journal of Surgical Pathology
https://read.qxmd.com/read/38503521/-clinical-characteristics-and-prognosis-of-newly-diagnosed-multiple-myeloma-patients-with-fgfr3-gene-mutations
#38
JOURNAL ARTICLE
N Shen, J Zhang, Y Xia, X X Shen, J Wang, Y Y Jin, R Zhang, J Y Li, L J Chen
Objective: This study aimed to investigate the influence of FGFR3 gene mutations on the clinical characteristics and prognosis of patients with newly diagnosed multiple myeloma (NDMM) . Methods: A total of 198 patients with NDMM admitted to the Department of Hematology in Jiangsu Province Hospital between January 2016 and February 2023 were retrospectively analyzed. Next-generation sequencing and cytoplasmic light chain immunofluorescence with fluorescence in situ hybridization were performed for all patients...
December 14, 2023: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://read.qxmd.com/read/38502492/dbd-fish-using-specific-chromosomal-region-probes-for-the-study-of-cervical-carcinoma-progression
#39
JOURNAL ARTICLE
Catalina García-Vielma, Elva I Cortés-Gutiérrez, José L Fernández, Martha I Dávila-Rodríguez, Jaime Gosálvez
Genomic instability is an important biomarker in the progression of cervical carcinoma. DBD-FISH (DNA breakage detection-fluorescence in situ hybridization) is a sensitive method that detects strand breaks, alkali-labile sites, and incomplete DNA excision repair in cells of the cervical epithelium. This technique integrates the microgel immersion of cells from a vaginal lesion scraping and the DNA unwinding treatment with the capacity of FISH integrated into digital image analysis. Cells captured within an agarose matrix are lysed and submerged in an alkaline unwinding solution that generates single-stranded DNA motifs at the ends of internal DNA strand breaks...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38502491/rapid-dna-fish-in-arabidopsis-thaliana-somatic-cells
#40
JOURNAL ARTICLE
Olga Raskina, Ofir Hakim
Fluorescence in situ hybridization (FISH) technique has been widely used to detect and localize specific DNA and RNA sequences in interphase nuclei and chromosomes in animals and plants. Here, we present a protocol for localization of genomic loci in nuclei of the model plant Arabidopsis thaliana. This protocol includes several advances and adaptations to A. thaliana, including preparation of nuclei and chromosomes without the use of liquid nitrogen, and an in situ hybridization procedure that preserves chromatin structure without the use of paraformaldehyde and formamide...
2024: Methods in Molecular Biology
keyword
keyword
100680
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.