Emily M Pujadas Liwag, Xiaolong Wei, Nicolas Acosta, Lucas M Carter, Jiekun Yang, Luay M Almassalha, Surbhi Jain, Ali Daneshkhah, Suhas S P Rao, Fidan Seker-Polat, Kyle L MacQuarrie, Joe Ibarra, Vasundhara Agrawal, Erez Lieberman Aiden, Masato T Kanemaki, Vadim Backman, Mazhar Adli
BACKGROUND: B-type lamins are critical nuclear envelope proteins that interact with the three-dimensional genomic architecture. However, identifying the direct roles of B-lamins on dynamic genome organization has been challenging as their joint depletion severely impacts cell viability. To overcome this, we engineered mammalian cells to rapidly and completely degrade endogenous B-type lamins using Auxin-inducible degron technology. RESULTS: Using live-cell Dual Partial Wave Spectroscopic (Dual-PWS) microscopy, Stochastic Optical Reconstruction Microscopy (STORM), in situ Hi-C, CRISPR-Sirius, and fluorescence in situ hybridization (FISH), we demonstrate that lamin B1 and lamin B2 are critical structural components of the nuclear periphery that create a repressive compartment for peripheral-associated genes...
March 22, 2024: Genome Biology