Li Zhang, Jinlong Li, Jinhua Liang, Zhenqiang Zhang, Qingjun Wei, Ke Wang
Background: Pseudomonas aeruginosa ( P. aeruginosa ) is a common pathogenic bacterium which causes pleural empyema, and infection of P. aeruginosa is often associated with biofilm. The aim of this study was to establish a model of rabbit empyema infected by P. aeruginosa to determine whether it causes the formation of biofilm in the pleural cavity. Furthermore, we investigated the effect of cyclic diguanosine monophosphate (c-di-GMP) on biofilm formation in this P. aeruginosa empyema model...
September 2020: Annals of Translational Medicine
Lars Iversen, Patrick Rene Gerhard Eriksen, Simon Andreasen, Erik Clasen-Linde, Preben Homøe, Irene Wessel, Christian von Buchwald, Steffen Heegaard
Background: Lymphoma of the sublingual gland is rare, representing 1% of all salivary gland lymphomas. In this case report, we present three new cases and compare them to previously published cases, with the aim of characterizing the clinical, morphological, histopathological, and genetic features of this type of malignancy. Materials and Methods: We provide a clinical description of three cases along with a characterization of the microscopic features, including morphology, and immunohistochemistry. In addition, we analysed possible cytogenetic rearrangements with the use of fluorescence in situ hybridization (FISH)...
2020: Frontiers in Surgery
Meijie Xu, Rui Wu, Ling Zhang, Hong-Yan Zhu, Guang-Yin Xu, Wenxia Qian, Ping-An Zhang
Background: Inflammatory pain is the most common type of pain treated clinically. However, the currently available treatments for inflammatory pain have limited effects and can cause severe side effects. The aim of this study is to describe the effect of miRNA-485-5p on osteoarthritis-related inflammatory pain. Methods: Paw withdrawal threshold (PWT) of rats was measured by von Frey filaments. The expressions of miRNA-485-5p and acid-sensing ion channel 1 (ASIC1) in the dorsal root ganglion (DRG) were measured with real-time quantitative PCR and Western blotting analysis...
2020: Journal of Pain Research
Regina Kwon, Pedram Argani, Jonathan I Epstein, Kara A Lombardo, Xiaoming Wang, Phillip M Pierorazio, Rohit Mehra, Andres Matoso
Our recent study of early-onset unclassified eosinophilic renal cell carcinoma (RCC) demonstrated that two third of cases could be reclassified by performing a limited number of immunohistochemistry stains. Following the same approach, we aimed to investigate what proportion of adult unclassified RCC could be reclassified. We identified 79 cases. The mean age at presentation was 58 years (range, 29 to 84 y). Tumors were grouped based on their predominant morphologic features as oncocytic (n=23); papillary (n=22); clear cell (n=22); mucinous tubular and spindle cell (MTSC; n=5); rhabdoid (n=4); or lacking a dominant pattern (n=3)...
November 24, 2020: American Journal of Surgical Pathology
Shuifang Chen, Yinan Yao, Shan Lu, Junjun Chen, Guangdie Yang, Lingfang Tu, Lina Chen
BACKGROUND: Dysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD). The present investigation elucidated the potential role of CircRNA0001859 in regulating chronic obstructive pulmonary disease acute (COPD) and Acute Exacerbation of COPD (AECOPD). METHODS: Mice model of COPD was established to screen and verify the dysregulated expression of CircRNA0001859. Fluorescence in situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR) were carried out to detect the expression of CircRNA0001859...
November 25, 2020: BMC Pulmonary Medicine
Michael Ozga, Qiuhong Zhao, Don Benson, Patrick Elder, Nita Williams, Naresh Bumma, Ashley Rosko, Maria Chaudhry, Abdullah Khan, Srinivas Devarakonda, Rami Kahwash, Ajay Vallakati, Courtney Campbell, Samir V Parikh, Salem Almaani, Jason Prosek, Jordan Bittengle, Katherine Pfund, Samantha LoRusso, Miriam Freimer, Elyse Redder, Yvonne Efebera, Nidhi Sharma
BACKGROUND: Autologous stem cell transplantation (ASCT) remains an effective treatment option for many patients with systemic light chain (AL) amyloidosis. While maintenance post ASCT in multiple myeloma is now standard, the decision to utilize maintenance in AL amyloidosis remains largely unexplored. The present study aims to determine the prognostic significance of utilizing maintenance therapy following ASCT and assess the impact of fluorescent in situ hybridization (FISH) abnormalities, bone marrow plasma cell burden (BMPC), and degree of organ involvement on this decision...
November 23, 2020: Journal of Clinical Medicine
Kanae Okada, Taishi Takahara, Yuka Suzuki, Kei Kohno, Ayako Sakakibara, Akira Satou, Emiko Takahashi, Shigeo Nakamura
Histiocytic and dendritic cell (H/DC) neoplasms are heterogeneous, originating from myeloid- or stromal-derived cells. Multiple reports describe the cross-lineage transdifferentiation of neoplastic B cells into H/DC neoplasms. Most such cases are from Western countries, and rarely from Japan or East Asia. Here we report 17 cases of H/DC neoplasms in Japanese patients, with analysis of t(14;18) by fluorescence in situ hybridization, and of neoplastic programmed death-ligand 1 (PD-L1) expression by immunostaining (clones SP142, E1J2J, and 28-8)...
November 25, 2020: Pathology International
Laurent Duchatelet, Victoria C Moris, Taketeru Tomita, Jacques Mahillon, Keiichi Sato, Catherine Behets, Jérôme Mallefet
Despite its five meters length, the megamouth shark (Megachasma pelagios Taylor, Compagno & Struhsaker, 1983) is one of the rarest big sharks known in the world (117 specimens observed and documented so far). This filter-feeding shark has been assumed to be a luminous species, using its species-specific white band to produce bioluminescence as a lure trap. Another hypothesis was the use of the white band reflectivity to attract prey or for social recognition purposes. However, no histological study has ever been performed to confirm these assumptions so far...
2020: PloS One
Cristina Aguado, Cristina Teixido, Ruth Román, Roxana Reyes, Ana Giménez-Capitán, Elba Marin, Carlos Cabrera, Nuria Viñolas, Sergi Castillo, Silvia Muñoz, Ainara Arcocha, Laura López-Vilaró, Ivana Sullivan, Erika Aldeguer, Sonia Rodríguez, Irene Moya, Santiago Viteri, Andrés Felipe Cardona, Ramon Palmero, Cristina Sainz, Miguel Mesa-Guzmán, Maria D Lozano, Andrés Aguilar-Hernández, Alejandro Martínez-Bueno, María González-Cao, Elena Gonzalvo, William P J Leenders, Rafael Rosell, Luis M Montuenga, Aleix Prat, Miguel A Molina-Vila, Noemi Reguart
MET inhibitors have shown activity in non-small cell lung cancer patients (NSCLC) with MET amplification and exon 14 skipping (METΔex14). However, patient stratification is imperfect and thus response rates have varied widely. Here, we studied MET alterations in 474 advanced NSCLC patients by nCounter, an RNA-based technique, together with Next Generation Sequencing (NGS), fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) and reverse transcriptase polymerase chain reaction (RT-PCR), exploring correlation with clinical benefit...
November 25, 2020: Molecular Oncology
Aruna Nambirajan, Niteeka Gurung, Vaishali Suri, Chitra Sarkar, Amandeep Kumar, Manmohan Singh, Mehar Chand Sharma
INTRODUCTION: CNS embryonal tumors (CET) other than medulloblastomas (MB) and atypical teratoid/rhabdoid tumors (AT/RTs), previously designated as 'central nervous system primitive neuroectodermal tumors' ('CNS PNETs'), are a heterogenous subset of tumors with poorly defined diagnostic criteria. Other than the subset of embryonal tumor with multilayered rosettes (ETMR) defined by C19MC amplification, most CETs are diagnosed by exclusion of other molecularly defined entities and histological mimics including MB, AT/RTs, and high-grade gliomas, and termed as CET, not otherwise specified (NOS) in the 2016 WHO classification...
November 24, 2020: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
Wei Gao, Huina Guo, Min Niu, Xiwang Zheng, Yuliang Zhang, Xuting Xue, Yunfeng Bo, Xiaoya Guan, Zhongxun Li, Yujia Guo, Long He, Yu Zhang, Li Li, Jimin Cao, Yongyan Wu
BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the second most common malignant tumor in head and neck. Autophagy and circular RNAs (circRNAs) play critical roles in cancer progression and chemoresistance. However, the function and mechanism of circRNA in autophagy regulation of LSCC remain unclear. METHODS: The autophagy-suppressive circRNA circPARD3 was identified via RNA sequencing of 107 LSCC tissues and paired adjacent normal mucosal (ANM) tissues and high-content screening...
November 24, 2020: Molecular Cancer
Mi Zhou, Hui Hu, Yineng Han, Jie Li, Yang Zhang, Song Tang, Yu Yuan, Xiaonan Zhang
BACKGROUND: Periodontitis is a prevalent oral inflammatory disease, which can cause periodontal ligament to a local hypoxia environment. However, the mechanism of hypoxia associated long non-coding RNAs (lncRNAs) involved in periodontitis is still largely unknown. METHODS: Microarray was performed to detect the expression patterns of lncRNAs in 3 pairs of gingival tissues from patients with periodontitis and healthy controls. The expression of lncRNA 01126 (LINC01126), miR-518a-5p and hypoxia-inducible factor-1α (HIF-1α) in periodontal tissues and in human periodontal ligament cells (hPDLCs) under hypoxia was measured by quantitative real-time polymerase chain reaction or western blot...
November 24, 2020: Cell Proliferation
Matthew Zorkau, Yasmin Proctor-Kent, Rolando Berlinguer-Palmini, Andrew Hamilton, Zofia M Chrzanowska-Lightowlers, Robert N Lightowlers
Human mitochondria contain their own DNA (mtDNA) that encodes 13 proteins all of which are core subunits of oxidative phosphorylation (OXPHOS) complexes. To form functional complexes, these 13 components need to be correctly assembled with approximately 70 nuclear-encoded subunits that are imported following synthesis in the cytosol. How this complicated coordinated translation and assembly is choreographed is still not clear. Methods are being developed to determine whether all members of a particular complex are translated in close proximity, whether protein synthesis is clustered in submitochondrial factories, whether these align with incoming polypeptides, and if there is evidence for co-translational translation that is regulated and limited by the interaction of the incoming proteins with synthesis of their mtDNA-encoded partners...
2021: Methods in Molecular Biology
Caixia Lei, Shuo Zhang, Saijuan Zhu, Junping Wu, Min Xiao, Jing Zhou, Jing Fu, Yijuan Sun, Congjian Xu, Xiaoxi Sun
PURPOSE: To evaluate whether the morphologically normal spermatozoa selected for intracytoplasmic sperm injection (ICSI) under microscope had a higher rate of normal/balanced chromosome contents than that in the whole unselected sperm from reciprocal translocation carriers. METHODS: Five hundred unselected spermatozoa from each of 40 male translocation carriers were performed with fluorescence in situ hybridization (FISH), to determine the rates of gametes with different meiotic contents of translocated chromosomes...
November 23, 2020: Journal of Assisted Reproduction and Genetics
Vivien Miczán, Krisztina Kelemen, Judit R Glavinics, Zsófia I László, Benjámin Barti, Kata Kenesei, Máté Kisfali, István Katona
The molecular repertoire of the "Ca2+-signaling toolkit" supports the specific kinetic requirements of Ca2+-dependent processes in different neuronal types. A well-known example is the unique expression pattern of calcium-binding proteins, such as parvalbumin, calbindin, and calretinin. These cytosolic Ca2+-buffers control presynaptic and somatodendritic processes in a cell-type-specific manner and have been used as neurochemical markers of GABAergic interneuron types for decades. Surprisingly, to date no typifying calcium-binding proteins have been found in CB1 cannabinoid receptor/cholecystokinin (CB1/CCK)-positive interneurons that represent a large population of GABAergic cells in cortical circuits...
November 24, 2020: Cerebral Cortex
Feifan Wang, Mengjing Fan, Yueshu Cai, Xuejian Zhou, Shengcheng Tai, Yanlan Yu, Hongshen Wu, Yan Zhang, Jiaxin Liu, Shihan Huang, Ning He, Zhenghui Hu, Xiaodong Jin
Circular RNAs (circRNAs), a subclass of noncoding RNAs, are reportedly involved in the progression of various diseases. However, the exact role of circRIMS1, also termed hsa_circ_0132246, in human bladder cancer remains unknown. By performing RNA sequencing comparing bladder cell lines and normal uroepithelial cells, circRIMS1 was selected as a research object. We further verified by qRT-PCR that circRIMS1 is upregulated in both bladder cancer tissue and cell lines. Proliferation, colony-formation, Transwell migration, invasion, apoptosis, western blotting, and in vivo experiments were utilized to clarify the roles of circRIMS1, microRNA (miR)-433-3p, and cell cycle and apoptosis regulator 1 (CCAR1)...
December 4, 2020: Molecular Therapy. Nucleic Acids
Akiko Hiraiwa, Kou Matsui, Yumi Nakayama, Takao Komatsubara, Shinichi Magara, Yu Kobayashi, Moemi Hojo, Mitsuhiro Kato, Toshiyuki Yamamoto, Jun Tohyama
BACKGROUND: Pallister-Killian syndrome (PKS) is a rare disorder caused by the mosaic tetrasomy of chromosome 12p, and is characterized by facial dysmorphism, developmental delay, hypotonia and seizures. RESULTS: We report a patient with PKS showing unique polymicrogyria with calcification. He had delayed development and dysmorphic facial features including frontal bossing, hypertelorism, and high arched palate at 6 months of age. Neuroimaging revealed unilateral polymicrogyria with spot calcifications, which predominantly affected the right perisylvian region...
November 20, 2020: Brain & Development
Neda Ebrahimian, Fatemeh Montazeri, Mohammad Reza Sadeghi, Seyed Mehdi Kalantar, Kambiz Gilany, Mohannad Ali Khalili
Objective: The goal of the present study was to investigate the rate of chromosomal aneuploidies in surplus embryos after sex determination at the cleavage stage. Then, the same chromosomal aneuploidies were evaluated in blastocysts after extended culture. Methods: Sixty-eight surplus embryos were biopsied at the cleavage stage and incubated for an additional 3 days to allow them to reach the blastocyst stage. The embryos were reanalyzed via fluorescence in situ hybridization (FISH) to examine eight chromosomes (13, 15, 16, 18, 21, 22, X, and Y) in both cleavage-stage embryos and blastocysts...
November 19, 2020: Clinical and Experimental Reproductive Medicine
Tam T Quach, Harrison J Stratton, Rajesh Khanna, Pappachan E Kolattukudy, Jérome Honnorat, Kathrin Meyer, Anne-Marie Duchemin
Intellectual disability (ID) corresponds to several neurodevelopmental disorders of heterogeneous origin in which cognitive deficits are commonly associated with abnormalities of dendrites and dendritic spines. These histological changes in the brain serve as a proxy for underlying deficits in neuronal network connectivity, mostly a result of genetic factors. Historically, chromosomal abnormalities have been reported by conventional karyotyping, targeted fluorescence in situ hybridization (FISH), and chromosomal microarray analysis...
November 23, 2020: Acta Neuropathologica
Liang-Yan Chen, Lian Wang, Yue-Xiang Ren, Zheng Pang, Yao Liu, Xiao-Dong Sun, Jian Tu, Zheng Zhi, Yan Qin, Li-Na Sun, Jian-Ming Li
BACKGROUND: Circular RNA (circRNAs) and hypoxia have been found to play the key roles in the pathogenesis and progression of cancer including colorectal cancer (CRC). However, the expressions and functions of the specific circRNAs in regulating hypoxia-involved CRC metastasis, and the circRNAs that are relevant to regulate HIF-1α levels in CRC remain elusive. METHODS: qRT-PCR was used to detect the expression of circRNAs and mRNA in CRC cells and tissues. Fluorescence in situ hybridization (FISH) was used to analyze the location of circ-ERBIN...
November 23, 2020: Molecular Cancer
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