Jordan T Moore, Christopher G Wier, Luke R Lemmerman, Lilibeth Ortega-Pineda, Daniel J Dodd, William R Lawrence, Silvia Duarte-Sanmiguel, Kavya Dathathreya, Ludmila Diaz-Starokozheva, Hallie N Harris, Chandan K Sen, Ian L Valerio, Natalia Higuita-Castro, William David Arnold, Stephen J Kolb, Daniel Gallego-Perez
While gene and cell therapies have emerged as promising treatment strategies for various neurological conditions, heavy reliance on viral vectors can hamper widespread clinical implementation. Here, the use of tissue nanotransfection as a platform nanotechnology to drive nonviral gene delivery to nerve tissue via nanochannels, in an effective, controlled, and benign manner is explored. TNT facilitates plasmid DNA delivery to the sciatic nerve of mice in a voltage-dependent manner. Compared to standard bulk electroporation (BEP), impairment in toe-spread and pinprick response is not caused by TNT, and has limited to no impact on electrophysiological parameters...
September 16, 2020: Advanced Biosystems