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SLAS Technology

Dino Di Carlo
The next generation of therapies is moving beyond the use of small molecules and proteins to using whole cells. Compared with the interactions of small-molecule drugs with biomolecules, which can largely be understood through chemistry, cell therapies act in a chemical and physical world and can actively adapt to that world, amplifying complexity but also the potential for truly breakthrough impact. Although there has been success in introducing targeting proteins into cells to achieve a therapeutic effect, for example, chimeric antigen receptor (CAR) T cells, our ability to engineer cells is generally limited to introducing proteins, but not modulating large-scale traits or structures of cellular "machines," which play critical roles in disease...
March 27, 2019: SLAS Technology
William Janzen, Elizabeth Admirand, Jeffery Andrews, Matthew Boeckeler, Chatura Jayakody, Christina Majer, Garima Porwal, Surayya Sana, Samantha Unkuri, Andy Zaayenga
This paper has been written by the SLAS Sample Management Special Interest Group to serve as a guide to the best practices and methods in establishing and maintaining a high-quality sample management system. The topics covered are applicable to sample types ranging from small molecules to biologics to tissue samples. It has been put together using the collective experience of the authors in start-up companies, small pharma, agricultural research, IT, academia, biorepositories, and large pharma companies. Our hope is that sharing our experience will streamline the process of setting up a new sample management system and help others avoid some of the problems that we have encountered...
March 13, 2019: SLAS Technology
Hongu Meng, Antony Warden, Lulu Zhang, Ting Zhang, Yiyang Li, Ziyang Tan, Boqian Wang, Hongxia Li, Hui Jiang, Guangxia Shen, Yifan Hong, Xianting Ding
Mass cytometry (CyTOF) is a critical cell profiling tool in acquiring multiparameter proteome data at the single-cell level. A major challenge in CyTOF analysis is sample-to-sample variance arising from the pipetting process, staining variation, and instrument sensitivity. To reduce such variations, cell barcoding strategies that enable the combination of individual samples prior to antibody staining and data acquisition on CyTOF are often utilized. The most prevalent barcoding strategy is based on a binary scheme that cross-examines the existence or nonexistence of certain mass signals; however, it is limited by low barcoding efficiency and high cost, especially for large sample size...
March 11, 2019: SLAS Technology
Heidi Fleischer, Shalaka Joshi, Thomas Roddelkopf, Michael Klos, Kerstin Thurow
The demand for automation in the analytical laboratory is high. In contrast to well-automated bioscreening and high-throughput and high-content screening processes, analytical measurement procedures are complex in their structure and changing frequently. Not only do robotic units have to perform transportation or specific single tasks, but also flexible robots are needed to cover several tasks, including transportation and direct sample manipulation. Due to their human-like structure, dual-arm robots are predestined for analytical measurement processes...
February 28, 2019: SLAS Technology
David I Walsh, Marilene Pavan, Luis Ortiz, Scott Wick, Johanna Bobrow, Nicholas J Guido, Sarah Leinicke, Dany Fu, Shreya Pandit, Lucy Qin, Peter A Carr, Douglas Densmore
The advancement of synthetic biology requires the ability to create new DNA sequences to produce unique behaviors in biological systems. Automation is increasingly employed to carry out well-established assembly methods of DNA fragments in a multiplexed, high-throughput fashion, allowing many different configurations to be tested simultaneously. However, metrics are required to determine when automation is warranted based on factors such as assembly methodology, protocol details, and number of samples. The goal of our synthetic biology automation work is to develop and test protocols, hardware, and software to investigate and optimize DNA assembly through quantifiable metrics...
February 15, 2019: SLAS Technology
Jenna Wahbeh, Sarah Milkowski
The use of hydrazones presents an opportunity for enhancing drug delivery through site-specific drug release, including areas such as tumor tissue or thrombosis. Many researchers are experimenting on how to more efficiently form these hydrazones, specifically using heat and chemical catalysts. Hydrazones respond on the pH environment or are synthesized with particular functional groups of the hydrazone and are two of the many unique features that allow for their programmed drug release. Their flexibility allows them to be relevant in a diverse range of applications, from anti-inflammatory to anticancer to acting as a chelating agent...
February 11, 2019: SLAS Technology
Snehal Bhatt, Sue Crimmin, Jeffrey Gross, Elizabeth Nixon, Maggie Truong, Michael Weglos, Lorena Kallal
Recent advancements in science and engineering are revolutionizing our understanding of an individual's disease, and with this knowledge we are gaining an increasingly sophisticated understanding of how discovery can be transformed to deliver personalized medicines. To reach this future state, we must reengineer our approach to enable the use of more relevant human cellular models earlier in the drug discovery process. Stem cells and primary human cells represent more disease-relevant models than immortalized cell lines; however, due to both availability and cost, their use is limited in lead generation activities...
February 6, 2019: SLAS Technology
Victor Ong, Vincent Mei, Lin Cao, Kiana Lee, Eun Ji Chung
Cystic fibrosis is a genetic disease affecting more than 70,000 people worldwide. Caused by a mutation in the CFTR gene, cystic fibrosis can result in difficulty breathing, widespread bacterial infections, edema, malnutrition, pancreatitis, and death. Current drug-based treatments struggle to reach the site of action due to the thick mucus, and only manage symptoms such as blocked airways, lung infections, and limited ability to digest food. Nanotechnology opens up possibilities for improved treatment strategies by focusing on drug penetration through the mucus lining, eliminating resulting bacterial infections, and targeting the underlying genetic cause of the disease...
February 1, 2019: SLAS Technology
Loïc Binan, Elliot A Drobetsky, Santiago Costantino
Multiplexing strategies, which greatly increase the number of simultaneously measured parameters in single experiments, are now being widely implemented by both the pharmaceutical industry and academic researchers. Color has long been used to identify biological signals and, when combined with molecular barcodes, has substantially enhanced the depth of multiplexed sample characterization. Moreover, the recent advent of DNA barcodes has led to an explosion of innovative cell sequencing approaches. Novel barcoding strategies also show great promise for encoding spatial information in transcriptomic studies, and for precise assessment of molecular abundance...
February 1, 2019: SLAS Technology
Susan Crimmin, Sara Grab, Nicole Greenwood, Zofia Jordon, Stacy Quirin, Nadia Tournier
The discovery of new medicines has become increasingly more challenging and requires significant collaboration between pharma, biotech, academia, and technology to be successful. These partnerships necessitate the streamlined exchange of samples while adhering to the increasingly complex set of legal and proprietary restrictions, government legislation, and ethical considerations associated with samples. There is a significant volume of literature published on clinical sample compliance but little describing compliance aspects of discovery sample management...
January 30, 2019: SLAS Technology
Pierre Baillargeon, Kervin Coss-Flores, Fakhar Singhera, Justin Shumate, Hannah Williams, Lina DeLuca, Timothy P Spicer, Louis Scampavia
Microplates are an essential tool used in laboratories for storing research materials and performing assays. Many types of laboratory automation exist that greatly reduce the effort needed to utilize microplates; however, there are cases where the use of such automation is not feasible or practical. In these instances, researchers must work in an environment where liquid handling operations are performed manually with handheld pipetting devices. This type of work is tedious and error-prone as it relies on researchers to manually track a significant amount of metadata, including transfer volumes, plate barcodes, well contents, and well locations...
January 30, 2019: SLAS Technology
Tom Bretschneider, Can Ozbal, Markus Holstein, Martin Winter, Frank H Buettner, Sven Thamm, Daniel Bischoff, Andreas H Luippold
Label-free in vitro potency assays are an emerging field in drug discovery to enable more physiological conditions, to improve the readout quality, and to save time. For this approach mass spectrometry (MS) is a powerful technology to directly follow physiological processes. The speed of this methodology, however, was for a long time not compatible with chemiluminescence- or fluorescence-based assays. Recent advances in matrix-assisted laser desorption/ionization (MALDI) instrumentation paved the way for high-throughput MS analysis of label-free assays for large compound libraries, whereas electrospray ionization (ESI)-based mass spectrometers equipped with RapidFire autosamplers were limited to medium throughput...
January 30, 2019: SLAS Technology
Eun Ji Chung
No abstract text is available yet for this article.
April 2019: SLAS Technology
David Yeo, Tal Murthy
No abstract text is available yet for this article.
April 2019: SLAS Technology
Tal Murthy, David Yeo
No abstract text is available yet for this article.
February 2019: SLAS Technology
Richard M Eglen
No abstract text is available yet for this article.
February 2019: SLAS Technology
Richard M Eglen, Terry Reisine
Human induced pluripotent stem cells (HiPSCs) provide several advantages for drug discovery, but principally they provide a source of clinically relevant tissue. Furthermore, the use of HiPSCs cultured in three-dimensional (3D) systems, as opposed to traditional two-dimensional (2D) culture approaches, better represents the complex tissue architecture in vivo. The use of HiPSCs in 3D spheroid and organoid culture is now growing, but particularly when using myocardial, intestinal enteric nervous system, and retinal cell lines...
February 2019: SLAS Technology
Edward Kai-Hua Chow
No abstract text is available yet for this article.
February 2019: SLAS Technology
Aaron J Walck, Kristi R Harkins
For the purposes of high-throughput immunoassay screening, PerkinElmer's AlphaLISA technology offers many benefits over traditional enzyme-linked immunosorbant assay (ELISA). However, its 680 nm excitation wavelength coincides with a wavelength of peak photosynthetic pigment absorbance, hindering the technology's utility within the plant biotechnology industry. In assays containing photosynthetic matrices, it is proposed that excitation of chlorophyll leads to the production of singlet oxygen, which initiates a pigment-associated background signal, reducing assay sensitivity...
January 10, 2019: SLAS Technology
Wenjun Guo, Sunil Kumar, Frederik Görlitz, Edwin Garcia, Yuriy Alexandrov, Ian Munro, Douglas J Kelly, Sean Warren, Peter Thorpe, Christopher Dunsby, Paul French
We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore proteins (KPs) in live budding yeast cells. The low copy number of many KPs and their small spatial extent present significant challenges for the quantification of donor fluorescence lifetime in the presence of significant cellular autofluorescence and photobleaching. Automated FLIM data acquisition was controlled by µManager and incorporated wide-field time-gated imaging with optical sectioning to reduce background fluorescence...
January 10, 2019: SLAS Technology
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