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Italian Journal of Food Safety

Mehdi Nematbakhsh
No abstract text is available yet for this article.
December 31, 2018: Italian Journal of Food Safety
Selene Rubiola, Francesco Chiesa, Stefania Zanet, Tiziana Civera
Sarcocystis spp. are protozoan parasites with an obligatory two-host life cycle, with herbivores as intermediate hosts and carnivores as definitive hosts. Cattle are intermediate hosts for several species of Sarcocystis : indeed, in addition to S. cruzi , S. hirsuta and S. hominis , at least four new species were recently identified in bovine muscle: S. bovifelis , S. rommeli , S. bovini and S.heydorni . Since is not possible to unambiguously discriminate between S. hominis and the new species either morphologically or by the analysis of the 18S ribosomial (rRNA) gene, the aim of the present study was to use molecular techniques to discriminate cattle Sarcocystis species, taking advantage of the higher discriminative power of the Cytochrome C Oxidase subunit I mitochondrial (mtDNA COI) gene...
December 31, 2018: Italian Journal of Food Safety
Silvia Bonardi, Clotilde Silvia Cabassi, Simona Longhi, Federico Pia, Margherita Corradi, Stefano Gilioli, Erika Scaltriti
Wild boars ( Sus scrofa ) are increasing in several European countries, including Italy. In areas with intensive animal farming, like the Italian Emilia-Romagna region, they are likely to be exposed to antimicrobialresistant (AMR) bacteria of livestock origin. In 2017-2018, 108 mesenteric lymph nodes samples were collected from 108 wild boars hunted in Parma province, Emilia-Romagna region, to be tested for ESBL- and carbapenemase-producing Escherichia coli . One isolate (WB-21L) out of 108 (0.9%) was phenotypically confirmed as ESBLproducing E...
December 31, 2018: Italian Journal of Food Safety
Alessandra De Cesare, Antonio Parisi, Alex Lucchi, Loredana Capozzi, Angela Bianco, Frederique Pasquali, Gerardo Manfreda
The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitted to DNA extraction. Total DNA was sequenced in the 16S rRNA and reads analysed with MG-RAST to classify the colonising bacteria up to the genus level and compare each taxonomic group in terms of mean relative frequency of abundance in conventional and antibiotic free carcasses...
December 31, 2018: Italian Journal of Food Safety
Marta Caruso, Laura Latorre, Gianfranco Santagada, Rosa Fraccalvieri, Laura Maria Difato, Angela Miccolupo, Loredana Capozzi, Elisabetta Bonerba, Anna Mottola, Antonio Parisi
The aim of the present study was to assess the prevalence and genetic characteristics of Arcobacter spp. in bovine bulk tank milk produced in Apulia Region (Italy). Samples collected from 396 dairy farms, after enrichment in a selective broth, were subjected to an Arcobacter genus - specific Real Time PCR. Positive broths, previously filtered, were seeded on Karmali, MCCD and Columbia Blood Agar plates; presumptive Arcobacter spp. colonies were identified using an amplification and sequencing method and then characterized by Multi-Locus Sequence Typing (MLST)...
December 31, 2018: Italian Journal of Food Safety
Frederique Pasquali, Federica Palma, Marcello Trevisani, Antonio Parisi, Alex Lucchi, Alessandra De Cesare, Gerardo Manfreda
In the present study, the genetic relationships as well as the virulome and resistome of newly sequenced O26 and O157 Shiga-toxin producing E. coli (STEC) isolates, collected from dairy farms in Italy, were investigated in comparison to publicly available genomes collected worldwide. The whole genome of Italian isolates was sequenced on Illumina MiSeq Platform. Reads quality control, de novo draft genome assembly, species confirmation and the 7- loci Multi-Locus Sequence Type assignment were performed using INNUca pipeline...
December 31, 2018: Italian Journal of Food Safety
Stefania Maria Marotta, Filippo Giarratana, Anastasia Calvagna, Graziella Ziino, Alessandro Giuffrida, Antonio Panebianco
Domestic environment, in particular, kitchen setting is a well-established source of microbial contamination. Kitchen sponges represent an important vehicle of microbial transmission and maintenance of spoilage bacteria and pathogenic strains responsible for food borne diseases. The aim of this study was to evaluate the microbial communities of 100 'in-use' kitchen sponges, improving the knowledge on their role in cross-contamination in domestic environment and transmission of ESBLproducing strains. Sponges were processed for: aerobic mesophilic bacteria (AMB), Enterobacteriaceae (EB), yeasts and molds (YM), coagulase-positive staphylococci (CPS), micrococci (MCC), anaerobic sulfite reducing bacteria (ASR), and for the detection of Listeria monocytogenes , Salmonella spp...
December 31, 2018: Italian Journal of Food Safety
Gaetano Liuzzo, Stefano Bentley, Federica Giacometti, Silvia Piva, Andrea Serraino
The paper describes the terminology of risk in the view of food safety objectives, criteria, ranking and hierarchization: the terms Performance Criterion, Process Criteria, Product Criterion, Microbiological Criteria, Food Safety Objective, Performance standard, Food safety policy objectives, Risk Assessment Policies, Weighing, Precaution, Prevention, Precautionary principle, Prevention principle, Principle of separation between risk assessment and management, Rank, Ranking, Categorization, Ranking, Priorization, ALARA (As Low Reasonably Achievable), ALOP (Appropriate level of protection), Risk Management, Risk management in the public function are reported and discussed...
December 31, 2018: Italian Journal of Food Safety
Cinzia Daga, Simona Cau, Maria Giovanna Tilocca, Barbara Soro, Aldo Marongiu, Bruna Vodret
Fish is one of fourteen allergens that must be highlighted on the label within the ingredients list. The European regulation is very restrictive to allergens with zero tolerance. Therefore, it is important to establish sensitive and specific methods for detecting fish allergen. Applicability to detect and quantify fish allergen by droplet digital polymerase chain reaction (ddPCR) has been evaluated in this work. Genomic DNA of three species belonging to the most common fish families were analyzed. PCR primers were designed to amplify a 166 bp region of the 18S rRNA gene...
December 31, 2018: Italian Journal of Food Safety
M Naceur Haouet, Mauro Tommasino, Maria Lucia Mercuri, Ferdinando Benedetti, Sara Di Bella, Marisa Framboas, Stefania Pelli, M Serena Altissimi
The most direct way to estimate the shelf life of a product is to conduct simulation tests which are time consuming and expensive. Conversely, accelerated shelf life tests can be successfully used for stable products having long expected shelf life. The aim of the study was directed to verify the possibility to apply an accelerated shelf life test to perishable food products having a short-expected shelf life, such as a new ready-to-eat processed food preparation, composed mainly by cereals, tuna and chicken, packed in thermo-sealed trays and pasteurised...
December 31, 2018: Italian Journal of Food Safety
Rossana Roila, Raffaella Branciari, Benedetta Staccini, David Ranucci, Dino Miraglia, Maria Serena Altissimi, Maria Lucia Mercuri, Naceur M Haouet
Nitrate and nitrite content was determined on a total of 900 samples of vegetables and cured meat and the nitrite and nitrate exposure assessment was evaluated for central Italy population based on the food consumption data reported by the national dietary surveys. The highest average content of nitrate was detected in rocket salad (4415 mg/kg) and radish (3817 mg/kg) and for cured meat in "Bresaola" (188 mg/kg) and in Bacon (178 mg/kg). The nitrite content was negligible both in vegetables than in cured meat...
September 26, 2018: Italian Journal of Food Safety
Roberta Barrasso, Edmondo Ceci, Laura Stinga, Giuseppina Tantillo, Giancarlo Bozzo
Cadmium (Cd) concentrations were evaluated in the samples of kidney, liver and muscle of sixty-six buffaloes regularly slaughtered. Forty were raised in Campania, in the territory between the province of Naples and Caserta and twenty-six were bred in Apulia, in the province of Bari. Two aliquots were prepared for the renal and hepatic samples: one intended for the chemical analysis and the other one intended for histological investigations. Muscle samples were the subject of purely chemical investigation. In the group of forty animals raised in the Campania region, the limits imposed by EC Reg...
September 26, 2018: Italian Journal of Food Safety
Serena Santonicola, Maria Carmela Ferrante, Genni di Leo, Nicoletta Murru, Aniello Anastasio, Raffaelina Mercogliano
Diet represents the primary route for human exposure to bisphenol A (BPA). As endocrine disruptor (ED), BPA has raised concerns about its adverse effects on human health. Therefore, EFSA recommended a tolerable daily intake (t-TDI) of 4 μg/kg bw/day and the EU Regulation n. 2018/213 fixed a specific migration limit (SML) of 0.05 mg/kg for BPA in food from plastic materials intended to come in contact with food. BPA could be present in milk due to environmental contamination, and also as a result of the migration from contact materials used during milking and storage...
September 26, 2018: Italian Journal of Food Safety
Matthias Ziegler, Simon Rüegg, Roger Stephan, Claudia Guldimann
We tested the growth potential of Listeria monocytogenes on six RTE fruit products at low (4°C at the factory followed by 8°C retail/home storage) and abusive (4°C followed by 12°C) storage temperatures. Sliced coconut and fresh cut cantaloupe, as well as a fruit mix containing diced pineapple, cantaloupe, apples and grapes supported the growth of L. monocytogenes with a growth potential δ>0.5 log CFU/g over six days. Mangoes, a mix of diced kiwi, cantaloupe and pineapple as well as a mix of diced pineapple, mango, grapefruit, kiwi and pomegranate did not support a growth potential that exceeded 0...
September 26, 2018: Italian Journal of Food Safety
Gaetano Liuzzo, Stefano Bentley, Federica Giacometti, Silvia Piva, Andrea Serraino
The paper describes the terminology of risk assessment in the view of food safety: different aspects of social involvement are defined and discussed; the terms Document, Expert, Risk manager, Lay Knowledge, Participant, Participation, Citizens' involvement, Community of interest, Consultation, Trust and Social trust are presented. Also, the terms Adverse effects, Human Illness Source Attribution, Food hygiene, Emerging disease, Safety, Food security and Food safety are discussed.
September 26, 2018: Italian Journal of Food Safety
Rossana Roila, Andrea Valiani, Dino Miraglia, David Ranucci, Claudio Forte, Massimo Trabalza-Marinucci, Maurizio Servili, Michela Codini, Raffaella Branciari
Considering that many plant-derived substances show antioxidant and antimicrobial properties, natural antioxidant administered through feed in livestock animals could increase the shelf life of meat and meat products. The aim of this work was to study the effect of olive oil by-products on chicken meat lipid and protein oxidation and oxidative stability during storage. Two hundred and ninety-seven 22-day-old fast growing (Ross 308) female chicks were randomly assigned to three experimental grower-finisher diets: i) a basal control diet (CTR), ii) CTR diet supplemented with a low dosage (4...
September 26, 2018: Italian Journal of Food Safety
Nurul U Karim, Uzmaa S A A Siddiq, Mohd R M Razak, Mohamad K M Zainol, Mohd I Abdullah
An aqueous extraction of moringa ( Moringa oleifera ) leaves were prepared as the edible coats for keeping the quality of the giant freshwater prawn ( Macrobrachium rosenbergii ). In addition, the antioxidant properties and activity; total phenolic content (TPC), total flavonoid contents (TFC), free radical scavenging activity (DPPH), and ferric reducing antioxidant power (FRAP) of moringa leaves were also determined. The phenolic compounds and antioxidant properties in the moringa leaves are low; 16.14 mgGAEg-1 for TPC; 5...
September 26, 2018: Italian Journal of Food Safety
Claudia Grilli, Roberta Stocchi, Anna Rita Loschi, Fabrizio Conti, Stefano Rea
The pre -slaughter mortality was investigated on broilers, in the Mediterranean climate condition, considering the most significant risk factors as the journey length, waiting time, season and the space allowance in cages. At first, the pre -slaughter mortality was studied considering the totality of birds and then by examining in detail three broiler categories: large, medium and small size. The average dead on arrival (DOA) recorded on the totality of birds throughout the year was 0.38% and the values obtained in winter, spring, summer and autumn were 0...
September 26, 2018: Italian Journal of Food Safety
Nathalie Spoerry Serrano, Claudio Zweifel, Sabrina Corti, Roger Stephan
This study investigated the microbiological quality and presence of bacterial foodborne pathogens in 51 raw milk cheeses (mainly semihard and hard cheese) and 53 raw meat products (cured meat products and sausages) marketed at farm level. With regard to Enterobacteriaceae, Escherichia (E.) coli , and coagulase-positive staphylococci (CPS), the examined products were generally of a good microbiological quality. Enterobacteriaceae were found in seven cheeses (1.0×102 - 8.8×104 CFU/g) and one sausage (2.0×102 CFU/g)...
July 3, 2018: Italian Journal of Food Safety
Nicole Heini, Roger Stephan, Sophia Johler
Heated food is known to be often contaminated with B. cereus , leading to cases of diarrhoeal or emetic diseases. Battalion kitchens or army catering facilities present a food safety risk, as temperature abuse and long storage time can result in serious public health problems affecting a high number of served people. In contrast to civil catering facilities, no microbiological monitoring systems are currently implemented in Swiss military kitchens. In this study toxin gene profiles and cytotoxicity levels of 21 isolates of B...
July 3, 2018: Italian Journal of Food Safety
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