Xinlu Cai, Wanlian Xu, Yang Zheng, Sendi Wu, Rundong Zhao, Nian Wang, Yaqian Tang, Meilan Ke, Qianjin Kang, Linquan Bai, Buchang Zhang, Hang Wu
The biosynthesis of bioactive secondary metabolites, specifically antibiotics, is of great scientific and economic importance. The control of antibiotic production typically involves different processes and molecular mechanism. Despite numerous efforts to improve antibiotic yields, joint engineering strategies for combining genetic manipulation with fermentation optimization remain finite. Lincomycin A (Lin-A), a lincosamide antibiotic, is industrially fermented by Streptomyces lincolnensis . Herein, the leucine-responsive regulatory protein (Lrp)-type regulator SLCG_4846 was confirmed to directly inhibit the lincomycin biosynthesis, whereas indirectly controlled the transcription of SLCG_2919 , the first reported repressor in S...
March 2024: Synthetic and Systems Biotechnology