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Reports of Biochemistry & Molecular Biology

Majid Hoseini, Mehdi Sahmani, Farshad Foroughi, Yousef Khazaei Monfared, Mehdi Azad
Background: Hypercoagulable states (HS) can result from several different inherited and acquired disease conditions that cause abnormalities in the genes, proteins and cellular factors involved in the coagulation cascade. Novel insight into the molecular mechanisms involved in the coagulation pathways can provide a framework to develop improved therapeutics to treat patients with coagulation disorders. Therefore, investigating the genetic abnormalities present in patients with coagulation disorders can offer critical insight into disease pathogenesis...
January 2019: Reports of Biochemistry & Molecular Biology
Hoda Derakhshanian, Abolghassem Djazayery, Mohammad Hassan Javanbakht, Mohammad Reza Eshraghian, Abbas Mirshafiey, Mahnaz Zarei, Ehsan Alvandi, Ehsan Djalali, Mahmoud Djalali
Background: Diabetic nephropathy is one of the most important microvascular complications and a major cause of morbidity and mortality in diabetic patients. This study was designed to investigate the effect of vitamin D on the expression of three key genes involved in the development of diabetic nephropathy. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups. The first group served as control and the other two groups received intraperitoneal injections of 45 mg/kg STZ to develop diabetes...
January 2019: Reports of Biochemistry & Molecular Biology
Akbar Derakhshan, Javad Sadeghi Allah Abadi, Jalil Tavakkol-Afshari, Amin Reza Nikpoor, Ramin Daneshvar, Saeed Shokoohi Rad, Mohammad-Reza Ansari-Astaneh, Sadegh Ghafarian
Background: Glaucoma is one of the main causes of irreversible blindness. The most common type of glaucoma is primary open angle glaucoma (POAG). TGF-β2, the main TGF-β isoform in the eye, is critical for extracellular matrix production and angiogenesis. Genetic studies have shown that TGF-β2 gene ( TGFB2 ) polymorphisms affect its expression in the eye. The aim of this study was to investigate the presence of the TGFB2 rs991967 polymorphism in POAG, and the effect of this polymorphism on clinical characteristics in POAG patients...
January 2019: Reports of Biochemistry & Molecular Biology
Mahmoud Amini, Abdolali Pakdaman, Shima Shapoori, Ghasem Mosayebi
Background: Acute cholecystitis is defined as gallbladder inflammation caused by obstruction of the cystic duct. The pro-inflammatory cytokine, high mobility group box-1 (HMGB1), has been found to hold critical roles in the pathogenesis of several different inflammatory diseases. This study aimed to determine the relationship between HMGB1 and acute cholecystitis, and examine the potential for this cytokine as a biomarker for clinical diagnosis. Methods: The serum of 23 patients with severe acute cholecystitis, 45 patients with mild acute cholecystitis and 35 healthy subjects was collected and isolated from peripheral blood...
January 2019: Reports of Biochemistry & Molecular Biology
Ali Torkashvand, Fariborz Bahrami, Minoo Adib, Soheila Ajdary
Background: Lactic acid bacteria such as Lactococcus (L.) lactis are powerful tools that can function as live delivery vectors and heterologous protein expression hosts in development of novel vaccines. Pertussis toxin (PT) and filamentous hemagglutinin (FHA) are important virulence factors of Bordetella (B.) pertussis and constitute the major components of commercially available acellular pertussis (aP) vaccines. The purpose of the present study was to express F1S1 fusion protein, consisted of the N-terminal region of S1 subunit from PT and FHA type 1 immunodominant domain by L...
January 2019: Reports of Biochemistry & Molecular Biology
Hasan Mansouritorghabeh, Mojtaba Sankian, Abdol-Reza Varasteh, Malihe Moghadam, Bahman Khameneh, Reza Farid-Hosseini, Farahzad Jabbari-Azad
Background: The quality of extracts used in the skin prick test directly influences the interpretation of the test. Accordingly, the outcomes and effectiveness of immunotherapy for the management of IgE-mediated allergies depend on the quality of the extracts used. Excipients, which are pharmacologically inert ingredients, are intentionally added to the active ingredients. The aim of this study was to address optimum excipients for stability Platanus (P.) orientalis extract. Methods: In this study the excipients examined were l-lysine (20 mM), l-cysteine (20 mM), albumin (0...
January 2019: Reports of Biochemistry & Molecular Biology
Mostafa Shahmoradi, Peyman Faridifar, Reza Shapouri, Seyed Fazlollah Mousavi, Mahnaz Ezzedin, Bahman Mirzaei
Background: Among hospitalized patients, Staphylococcus aureus (S. aureus) infections pose a serious health threat. The present study investigated the frequency of biofilm forming genes among clinical isolates S. aureus and their susceptibility to antibiotics. Methods: The clinical samples were analyzed via standard biochemical assays for identifying different bacterium, which was then confirmed using the multiplex colony PCR method. Those samples identified as S...
January 2019: Reports of Biochemistry & Molecular Biology
Shobha Ullas Kamath, Abhishek Chaturvedi, Devesh Bhaskar Yerrapragada, Nagendra Kundapura, Navaneeth Amin, Virupaksha Devaramane
Background: Depression is a common and widespread mood disorder, which affects an emotional level that varies widely in its intensity. Biochemical parameter alterations have been observed in different depression types. In the present study, we examined acetylcholinesterase (AChE), paraoxonase 1 (PON1), and copper levels in moderately-depressed patients and healthy controls to ascertain whether the measurement of red blood cell (RBC) AChE, and plasma PON1 and copper could be used to evaluate moderate depression...
January 2019: Reports of Biochemistry & Molecular Biology
Akbar Derakhshan, Jalil Tavakkol Afshari, Javad Sadeghi Allah Abadi, Amin Reza Nikpoor, Ramin Daneshvar, Saeed Shokoohi Rad, Mohammad-Reza Ansari-Astaneh
Background: Glaucoma is a common cause of irreversible blindness. Transforming growth factor beta-1(TGF-β1) is the main isoform of TGF-β superfamily in the eye. Overexpression of TGF-β1 is shown to be related with the glaucoma. Studies have shown that the presence of mutant T allele of TGF-β1 -509C>T polymorphism (rs1800469) is associated with increased gene expression. So, in present study, association of the TGF-β1-509C>T gene polymorphism and primary open angle glaucoma (POAG) in patients from north east of Iran was investigated...
January 2019: Reports of Biochemistry & Molecular Biology
Davood Mansury, Kiarash Ghazvini, Saeid Amel Jamehdar, Ali Badiee, Mohsen Tafaghodi, Amin Reza Nikpoor, Yousef Amini, Mahmoud Reza Jaafari
Background: Due to the ineffectiveness of the BCG vaccine, especially in adult pulmonary tuberculosis (TB), and variable efficacies against childhood forms of TB, developing an effective TB vaccine is a major priority in controlling this disease. The aim of this study was to evaluate the immunogenicity of a DOTAP liposome formulation containing a fusion protein (FP) containing Mycobacterium tuberculosis HspX, PPE44, and EsxV. Methods: The FP was expressed in E. coli BL21 cells and confirmed by SDS-PAGE and Western blots...
January 2019: Reports of Biochemistry & Molecular Biology
Vahid Yaghoubi Naei, Mojtaba Sankian, Malihe Moghadam, Narges Farshidi, Seyed Hasan Ayati, Fatemeh Hamid, Abdol-Reza Varasteh
Background: Gamma irradiation is a form of processing with an array of applications in medical sciences such as microbial decontamination, viruses inactivation, cervical carcinoma and breast cancer treatment. One of the ways in which gamma irradiation has the potential to be used is in reducing the allergenicity of food allergens. Methods: In the present study, pistachios were irradiated with either a 1, 10, or 100 kGy dose of gamma irradiation. The binding rate of mice and human antibodies to the allergens of the pistachio extracts were examined via Western blot analysis...
January 2019: Reports of Biochemistry & Molecular Biology
Farnaz Mohajertehran, Hossein Ayatollahi, Amir Hossein Jafarian, Kamran Khazaeni, Mohammad Soukhtanloo, Mohammad-Taghi Shakeri, Nooshin Mohtasham
Background: Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignancy that is associated with high morbidity and mortality. Salivary lactate dehydrogenase (LDH concentration), as an expression of cellular necrosis, may be a special marker of lesions that occur with changes in the integrity of the oral mucosa. This study was performed to determine the accuracy of salivary LDH as a clinical marker for HNSCC detection and to investigate the relationship between salivary LDH levels and tissue tumor detection...
January 2019: Reports of Biochemistry & Molecular Biology
Milad Gholami, Sahar Bayat, Saba Manoochehrabadi, Hossein Pashaiefar, Mir Davood Omrani, Hasan Jalaeikhoo, Vahid Reza Yassaee, Mohammad Reza Ebrahimpour, Farkhondeh Behjati, Reza Mirfakhraie
Background: The pathogenicity of acute myeloid leukemia (AML) is highly influenced by genetic alterations, such as chromosomal abnormalities. Additionally, aberrations in the mechanisms involved in gene expression have been identified to have a role in the development of AML. Contradictory evidence has been reported concerning the expression of the CEBPA gene in AML patients. Additionally, investigation into the expression of the CEBPA-AS gene has yet to be explored in AML patients. The aim of the present study was to investigate the relationship between the expression of the CEBPA and CEBPA-AS genes and AML in Iranian patients...
January 2019: Reports of Biochemistry & Molecular Biology
Mojgan Mohammadi, Shahriar Dabiri, Hamid Reza Mollaei, Samira Rezaee Jouzdani, Maryam Amizadeh, Jamshid Esmailzadeh, Mohammad Reza Baneshi, Aliasghar Arabi Mianroodi
Background: Recent studies have shown interleukin 4 (IL-4) and 5 lipoxygenase (5-LO) to play an important role in development of nasal polyposis. Investigation into the genetic factors associated with allergic and non-allergic nasal polyposis has been examined for more than fifteen years. Despite these efforts, the genetic factors underlying the development of nasal polyposis have yet to be clearly understood. The current study examined the relationship between C-590T promoter polymorphisms of the IL-4 gene and the presence of nasal polyps...
January 2019: Reports of Biochemistry & Molecular Biology
Armin Mokhtariye, Lida Hagh-Nazari, Abdol-Reza Varasteh, Fatemeh Keyfi
Lysosomal storage disorders (LSD) are a class of metabolic disturbance in which manifested by the accumulation of large molecules (complex lipids, glycoproteins, glycosaminoglycans, etc.) in lysosomes. LSDs have a wide range of clinical symptoms that may contain organ dysfunction, neurological and skeletal disorders. The first stage of diagnosis is clinically suspected by a physician. Next stage is enzyme activity assays including Fluorometry and MS/MS methods. These methods usually placed in newborn program screening...
January 2019: Reports of Biochemistry & Molecular Biology
Munesh Kumar, Rajesh Thakur
Background: Arsenic is a well-documented human carcinogen widely distributed in the environment. Chronic exposure of humans to inorganic arsenicals causes many adverse health effects. The present work was conducted to evaluate the protective effect of Syzygium cumini seed extract (SCE) on arsenic-induced genotoxicity and hepatotoxicity in Wistar albino rats. Methods: Rats were randomly divided into five groups of six animals each. Group 1 served as normal control, Group 2 received SCE, 200 mg/kg daily, and Group 3 received arsenic, 100 ppm in drinking water...
October 2018: Reports of Biochemistry & Molecular Biology
Naghmeh Ahmadiankia, Mehdi Bagheri, Mozhgan Fazli
Background: Triple-negative breast cancer (TNBC) is treated with highly aggressive non-targeted chemotherapies. Safer and more effective therapeutic approaches than those currently in use are needed. Natural pomegranate peel extract (PPE) has recently been found to inhibit breast cancer progression; however, its mechanisms of action remain unclear. We hypothesized that transcriptional changes in the genes encoding the adherence proteins of intercellular adhesion molecule-1 (ICAM-1) and vascular endothelial growth factor (VEGF), may explain, at least in part, the anti-metastatic properties of PPE...
October 2018: Reports of Biochemistry & Molecular Biology
Mehdi Koushki, Nasrin Amiri Dash Atan, Hossein Omidi-Ardali, Mostafa Rezaei Tavirani
Background: Pre-eclampsia (PE) is a pregnancy disorder characterized by hypertension and proteinuria. The evidence has suggested that microRNAs (miRs) are associated with pre-eclampsia pathogenesis; however, these results are inconsistent. The aim of this study was to assess the association between miR-210 expression and PE risk. Methods: Previous studies were selected using PubMed, Scopus, MEDLINE, EMBASE, Science Direct, Google Scholar, Directory of Open Access Journals (DOAJ), and Scientific Information Database (SID)...
October 2018: Reports of Biochemistry & Molecular Biology
Nasiri Sayed Mahdi, Farideh Azarbani, Afshin Pirnia, Abolfazl Abbaszadeh, Mohammadreza Gholami
Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. However, during this process, the stem cells often become damaged which degrades their value for experiments and treatments. Caffeic acid (CA) is an antioxidant that has been shown to increase the viability of cells under stress...
October 2018: Reports of Biochemistry & Molecular Biology
Rajappa Rashmi, Salai Bojan Magesh, Kunka Mohanram Ramkumar, Sarvajayakesavulu Suryanarayanan, Madhunapantula Venkata SubbaRao
Background: Naringenin is a bioactive flavonoid found in grapes and citrus fruits including tangelo, blood orange, lemons, and tangerines. The aims of this study were to investigate the ability of naringenin to scavenge free radicals and determine its ability to protect animals from streptozotocin (STZ) -induced liver damage. Methods: The free radical-scavenging activity of naringenin was evaluated by in vitro cell-free assay systems. In animals, the antioxidant potential of orally administered 50 and 100 mg/kg body weight naringenin for 45 days was assessed by measuring TBARS, lipid hydroperoxides, SOD, catalase, GST, GPx, and glutathione levels in liver homogenates prepared from animals injected intraperitoneally with multiple low dose streptozotocin at 50 mg/kg for five consecutive days...
October 2018: Reports of Biochemistry & Molecular Biology
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