Elze Rackaityte, Irina Proekt, Haleigh S Miller, Akshaya Ramesh, Jeremy F Brooks, Andrew F Kung, Caleigh Mandel-Brehm, David Jl Yu, Colin R Zamecnik, Rebecca Bair, Sara E Vazquez, Sara Sunshine, Clare L Abram, Clifford A Lowell, Gabrielle Rizzuto, Michael R Wilson, Julie Zikherman, Mark S Anderson, Joseph L DeRisi
Autoimmunity is characterized by loss of tolerance to tissue-specific as well as systemic antigens, resulting in complex autoantibody landscapes. Here, we introduce and extensively validate the performance characteristics of a murine proteome-wide library for phage display immunoprecipitation and sequencing (PhIP-seq), to profile mouse autoantibodies. This library was validated using seven genetic mouse lines across a spectrum of autoreactivity. Mice deficient in antibody production (Rag2-/- and µMT) were used to model non-specific peptide enrichments, while cross-reactivity was evaluated using anti-ovalbumin B cell receptor (BCR)-restricted OB1 mice as a proof of principle...
November 7, 2023: JCI Insight