journal
https://read.qxmd.com/read/38809064/temporal-gene-expression-during-asexual-development-of-the-apicomplexan-sarcocystis-neurona
#1
JOURNAL ARTICLE
Sriveny Dangoudoubiyam, Jamie K Norris, Sivaranjani Namasivayam, Rodrigo de Paula Baptista, Naila Cannes do Nascimento, Joseph Camp, Christopher L Schardl, Jessica C Kissinger, Daniel K Howe
UNLABELLED: Asexual replication in the apicomplexan Sarcocystis neurona involves two main developmental stages: the motile extracellular merozoite and the sessile intracellular schizont. Merozoites invade host cells and transform into schizonts that undergo replication via endopolygeny to form multiple (64) daughter merozoites that are invasive to new host cells. Given that the capabilities of the merozoite vary significantly from the schizont, the patterns of transcript levels throughout the asexual lifecycle were determined and compared in this study...
May 29, 2024: MSphere
https://read.qxmd.com/read/38780289/a-novel-clinical-metaproteomics-workflow-enables-bioinformatic-analysis-of-host-microbe-dynamics-in-disease
#2
JOURNAL ARTICLE
Katherine Do, Subina Mehta, Reid Wagner, Dechen Bhuming, Andrew T Rajczewski, Amy P N Skubitz, James E Johnson, Timothy J Griffin, Pratik D Jagtap
UNLABELLED: Clinical metaproteomics has the potential to offer insights into the host-microbiome interactions underlying diseases. However, the field faces challenges in characterizing microbial proteins found in clinical samples, usually present at low abundance relative to the host proteins. As a solution, we have developed an integrated workflow coupling mass spectrometry-based analysis with customized bioinformatic identification, quantification, and prioritization of microbial proteins, enabling targeted assay development to investigate host-microbe dynamics in disease...
May 23, 2024: MSphere
https://read.qxmd.com/read/38775467/what-s-on-a-prophage-analysis-of-salmonella-spp-prophages-identifies-a-diverse-range-of-cargo-with-multiple-virulence-and-metabolism-associated-functions
#3
JOURNAL ARTICLE
Caroline R Yates, Anthony Nguyen, Jingqiu Liao, Rachel A Cheng
UNLABELLED: The gain of mobile elements, such as prophages, can introduce cargo to the recipient bacterium that could facilitate its persistence in or expansion to a new environment, such as a host. While previous studies have focused on identifying and characterizing the genetic diversity of prophages, analyses characterizing the cargo that prophages carry have not been extensively explored. We characterized prophage regions from 303 Salmonella spp. genomes (representing 254 unique serovars) to assess the distribution of prophages in diverse Salmonella ...
May 22, 2024: MSphere
https://read.qxmd.com/read/38771096/correction-for-ghoshal-et-al-9-o-acetylated-sialoglycoproteins-are-important-immunomodulators-in-indian-visceral-leishmaniasis
#4
JOURNAL ARTICLE
Angana Ghoshal, Sumi Mukhopadhyay, Bibhuti Saha, Chitra Mandal
No abstract text is available yet for this article.
May 21, 2024: MSphere
https://read.qxmd.com/read/38771057/the-trophoblast-surface-becomes-refractory-to-adhesion-by-congenitally-transmitted-toxoplasma-gondii-and-listeria-monocytogenes-during-cytotrophoblast-to-syncytiotrophoblast-development
#5
JOURNAL ARTICLE
Rafaela J da Silva, Leah F Cabo, Jada L George, Laty A Cahoon, Liheng Yang, Carolyn B Coyne, Jon P Boyle
The placenta is a critical barrier against viral, bacterial, and eukaryotic pathogens. For most teratogenic pathogens, the precise molecular mechanisms of placental resistance are still being unraveled. Given the importance of understanding these mechanisms and challenges in replicating trophoblast-pathogen interactions using in vitro models, we tested an existing stem-cell-derived model of trophoblast development for its relevance to infection with Toxoplasma gondii . We grew human trophoblast stem cells (TSCT ) under conditions leading to either syncytiotrophoblast (TSSYN ) or cytotrophoblast (TSCYT ) and infected them with T...
May 21, 2024: MSphere
https://read.qxmd.com/read/38771049/cryptic-environmental-conjugative-plasmid-recruits-a-novel-hybrid-transposon-resulting-in-a-new-plasmid-with-higher-dispersion-potential
#6
JOURNAL ARTICLE
Iván Muñoz-Gutiérrez, Luis Cantu, Jack Shanahan, Miray Girguis, Marlene de la Cruz, Luis Mota-Bravo
UNLABELLED: Cryptic conjugative plasmids lack antibiotic-resistance genes (ARGs). These plasmids can capture ARGs from the vast pool of the environmental metagenome, but the mechanism to recruit ARGs remains to be elucidated. To investigate the recruitment of ARGs by a cryptic plasmid, we sequenced and conducted mating experiments with Escherichia coli SW4848 (collected from a lake) that has a cryptic IncX (IncX4) plasmid and an IncF (IncFII/IncFIIB) plasmid with five genes that confer resistance to aminoglycosides ( strA and strB ), sulfonamides ( sul2 ), tetracycline [ tet ( A )], and trimethoprim ( dfrA5 )...
May 21, 2024: MSphere
https://read.qxmd.com/read/38771036/human-plasma-like-medium-hplm-induces-cryptococcus-neoformans-in-vivo-cell-morphologies
#7
JOURNAL ARTICLE
Eduardo G Mozo, Orlando Ross, Raif Yuecel, Ivy M Dambuza, Liliane Mukaremera
The fungal pathogen Cryptococcus neoformans (C. neoformans ) forms yeast cells of different sizes and morphological characteristics during infection. These features are usually not seen in standard laboratory in vitro conditions. Here, we describe in vivo cell morphologies when C. neoformans is grown in human plasma-like medium at 37°C, 5% CO2 . We observed mixed-size populations of cells less than 1 µm up to 16.8 µm in cell diameter, increased capsule size, high chitin, and DNA content in larger cells...
May 21, 2024: MSphere
https://read.qxmd.com/read/38771035/phenotypic-characterization-of-cryptic-species-in-the-fungal-pathogen-histoplasma
#8
JOURNAL ARTICLE
Victoria E Sepúlveda, Jonathan A Rader, Jingbaoyi Janet Li, William E Goldman, Daniel R Matute
Histoplasmosis is an endemic mycosis that often presents as a respiratory infection in immunocompromised patients. Hundreds of thousands of new infections are reported annually around the world. The etiological agent of the disease, Histoplasma, is a dimorphic fungus commonly found in the soil where it grows as mycelia. Humans can become infected by Histoplasma through inhalation of its spores (conidia) or mycelial particles. The fungi transition into the yeast phase in the lungs at 37°C. Once in the lungs, yeast cells reside and proliferate inside alveolar macrophages...
May 21, 2024: MSphere
https://read.qxmd.com/read/38771028/bioinformatic-prediction-of-proteins-relevant-to-functions-of-the-bacterial-ole-ribonucleoprotein-complex
#9
JOURNAL ARTICLE
Chrishan M Fernando, Ronald R Breaker
OLE (ornate, large, extremophilic) RNAs are members of a noncoding RNA class present in many Gram-positive, extremophilic bacteria. The large size, complex structure, and extensive sequence conservation of OLE RNAs are characteristics consistent with the hypothesis that they likely function as ribozymes. The OLE RNA representative from Halalkalibacterium halodurans is known to localize to the phospholipid membrane and requires at least three essential protein partners: OapA, OapB, and OapC. However, the precise biochemical functions of this unusual ribonucleoprotein (RNP) complex remain unknown...
May 21, 2024: MSphere
https://read.qxmd.com/read/38757961/mammalian-orthoreovirus-capsid-protein-%C3%AF-3-antagonizes-rlr-mediated-antiviral-responses-by-degrading-mavs
#10
JOURNAL ARTICLE
Dianyu Li, Rongqian Mo, Xiaoyi Li, Rongrong Cheng, Jingying Xie, Hongshan Li, Yanmei Yang, Shasha Li, Huixia Li, Zhenfang Yan, Suocheng Wei, Adi Idris, Xiangrong Li, Ruofei Feng
UNLABELLED: Mammalian orthoreovirus (MRV) outer capsid protein σ3 is a multifunctional protein containing a double-stranded RNA-binding domain, which facilitates viral entry and assembly. We reasoned that σ3 has an innate immune evasion function. Here, we show that σ3 protein localizes in the mitochondria and interacts with mitochondrial antiviral signaling protein (MAVS) to activate the intrinsic mitochondria-mediated apoptotic pathway. Consequently, σ3 protein promotes the degradation of MAVS through the intrinsic caspase-9/caspase-3 apoptotic pathway...
May 17, 2024: MSphere
https://read.qxmd.com/read/38752757/defective-pgsa-contributes-to-increased-membrane-fluidity-and-cell-wall-thickening-in-staphylococcus-aureus-with-high-level-daptomycin-resistance
#11
JOURNAL ARTICLE
Christian D Freeman, Tayte Hansen, Ramona Urbauer, Brian J Wilkinson, Vineet K Singh, Kelly M Hines
UNLABELLED: Daptomycin is a membrane-targeting last-resort antimicrobial therapeutic for the treatment of infections caused by methicillin- and/or vancomycin-resistant Staphylococcus aureus . In the rare event of failed daptomycin therapy, the source of resistance is often attributable to mutations directly within the membrane phospholipid biosynthetic pathway of S. aureus or in the regulatory systems that control cell envelope response and membrane homeostasis. Here we describe the structural changes to the cell envelope in a daptomycin-resistant isolate of S...
May 16, 2024: MSphere
https://read.qxmd.com/read/38752729/prediction-by-genetic-mats-of-4cmenb-vaccine-strain-coverage-of-invasive-meningococcal-serogroup-b-isolates-circulating-in-taiwan-between-2003-and-2020
#12
JOURNAL ARTICLE
Alessandro Muzzi, Min-Chi Lu, Elena Mori, Alessia Biolchi, Tiffany Fu, Laura Serino
Neisseria meningitidis serogroup B (NmB) strains have diverse antigens, necessitating methods for predicting meningococcal serogroup B (MenB) vaccine strain coverage. The genetic Meningococcal Antigen Typing System (gMATS), a correlate of MATS estimates, predicts strain coverage by the 4-component MenB (4CMenB) vaccine in cultivable and non-cultivable NmB isolates. In Taiwan, 134 invasive, disease-causing NmB isolates were collected in 2003-2020 (23.1%, 4.5%, 5.2%, 29.8%, and 37.3% from individuals aged ≤11 months, 12-23 months, 2-4 years, 5-29 years, and ≥30 years, respectively)...
May 16, 2024: MSphere
https://read.qxmd.com/read/38747609/msphere-of-influence-retreading-old-ground-the-beauty-in-multi-omic-data-sets
#13
JOURNAL ARTICLE
Ethan L Morgan
Ethan L. Morgan works on human papillomaviruses (HPVs), with a specific interest in identifying how HPV induces tumor formation. In this mSphere of Influence article, he reflects on how three papers influenced him. "Comprehensive genomic characterization of head and neck squamous cell carcinomas" (The Cancer Genome Atlas Network, Nature 517:576-582, 2015, https://doi.org/10.1038/nature14129) and "Integrated genomic and molecular characterization of cervical cancer" (The Cancer Genome Atlas Network, Nature 543: 378-384, 2017, https://doi...
May 15, 2024: MSphere
https://read.qxmd.com/read/38747590/integrated-hfq-interacting-rnaome-and-transcriptomic-analysis-reveals-complex-regulatory-networks-of-nitrogen-fixation-in-root-associated-pseudomonas-stutzeri-a1501
#14
JOURNAL ARTICLE
Fanyang Lv, Yuhua Zhan, Haichao Feng, Wenyue Sun, Changyan Yin, Yueyue Han, Yahui Shao, Wei Xue, Shanshan Jiang, Yiyuan Ma, Haonan Hu, Jinfeng Wei, Yongliang Yan, Min Lin
UNLABELLED: The RNA chaperone Hfq acts as a global regulator of numerous biological processes, such as carbon/nitrogen metabolism and environmental adaptation in plant-associated diazotrophs; however, its target RNAs and the mechanisms underlying nitrogen fixation remain largely unknown. Here, we used enhanced UV cross-linking immunoprecipitation coupled with high-throughput sequencing to identify hundreds of Hfq-binding RNAs probably involved in nitrogen fixation, carbon substrate utilization, biofilm formation, and other functions...
May 15, 2024: MSphere
https://read.qxmd.com/read/38742900/correction-for-dong-et-al-genomic-revisitation-and-reclassification-of-the-genus-providencia
#15
JOURNAL ARTICLE
Xu Dong, Huiqiong Jia, Yuyun Yu, Yanghui Xiang, Ying Zhang
No abstract text is available yet for this article.
May 14, 2024: MSphere
https://read.qxmd.com/read/38742887/a-shared-group-of-bacterial-taxa-in-the-duodenal-microbiota-of-undernourished-pakistani-children-with-environmental-enteric-dysfunction
#16
JOURNAL ARTICLE
Najeeha T Iqbal, Robert Y Chen, Nicholas W Griffin, Matthew C Hibberd, Aqsa Khalid, Kamran Sadiq, Zehra Jamil, Kumail Ahmed, Junaid Iqbal, Aneeta Hotwani, Furqan Kabir, Najeeb Rahman, Arjumand Rizvi, Romana Idress, Zubair Ahmed, Sheraz Ahmed, Fayaz Umrani, Sana Syed, Sean R Moore, Asad Ali, Michael J Barratt, Jeffrey I Gordon
Environmental enteric dysfunction (EED) is a subclinical syndrome of altered small intestinal function postulated to be an important contributor to childhood undernutrition. The role of small intestinal bacterial communities in the pathophysiology of EED is poorly defined due to a paucity of studies where there has been a direct collection of small intestinal samples from undernourished children. Sixty-three members of a Pakistani cohort identified as being acutely malnourished between 3 and 6 months of age and whose wasting (weight-for-length Z -score [WLZ]) failed to improve after a 2-month nutritional intervention underwent esophagogastroduodenoscopy (EGD)...
May 14, 2024: MSphere
https://read.qxmd.com/read/38738873/restoring-colistin-sensitivity-in-colistin-resistant-salmonella-and-escherichia-coli-combinatorial-use-of-berberine-and-edta-with-colistin
#17
JOURNAL ARTICLE
Xiao-Die Cui, Xiao-Kang Liu, Xiao-Yuan Ma, Shuai-Hua Li, Jun-Kai Zhang, Rong-Jia Han, Kai-Fang Yi, Jian-Hua Liu, Yu-Shan Pan, Dan-Dan He, Gong-Zheng Hu, Ya-Jun Zhai
UNLABELLED: The appearance and prevalence of multidrug-resistance (MDR) Gram-negative bacteria (GNB) have limited our antibiotic capacity to control bacterial infections. The clinical efficacy of colistin (COL), considered as the "last resort" for treating GNB infections, has been severely hindered by its increased use as well as the emergence and prevalence of mobile colistin resistance (MCR)-mediated acquired drug resistance. Identifying promising compounds to restore antibiotic activity is becoming an effective strategy to alleviate the crisis of increasing MDR...
May 13, 2024: MSphere
https://read.qxmd.com/read/38722162/the-unusual-mode-of-action-of-the-polyketide-glycoside-antibiotic-cervimycin-c
#18
JOURNAL ARTICLE
Alina Hoffmann, Ursula Steffens, Boris Maček, Mirita Franz-Wachtel, Kay Nieselt, Theresa Anisja Harbig, Kirstin Scherlach, Christian Hertweck, Hans-Georg Sahl, Gabriele Bierbaum
Cervimycins A-D are bis-glycosylated polyketide antibiotics produced by Streptomyces tendae HKI 0179 with bactericidal activity against Gram-positive bacteria. In this study, cervimycin C (CmC) treatment caused a spaghetti-like phenotype in Bacillus subtilis 168, with elongated curved cells, which stayed joined after cell division, and exhibited a chromosome segregation defect, resulting in ghost cells without DNA. Electron microscopy of CmC-treated Staphylococcus aureus (3 × MIC) revealed swollen cells, misshapen septa, cell wall thickening, and a rough cell wall surface...
May 9, 2024: MSphere
https://read.qxmd.com/read/38712970/comprehensive-lipidomic-analysis-of-the-genus-cutibacterium
#19
JOURNAL ARTICLE
Anna Chudzik, Mariusz A Bromke, Andrzej Gamian, Mariola Paściak
UNLABELLED: Cutibacterium are part of the human skin microbiota and are opportunistic microorganisms that become pathogenic in immunodeficient states. These lipophilic bacteria willingly inhabit areas of the skin where sebaceous glands are abundant; hence, there is a need to thoroughly understand their metabolism. Lipids are no longer considered only structural elements but also serve as signaling molecules and may have antigenic properties. Lipidomics remains a major research challenge, mainly due to the diverse physicochemical properties of lipids...
May 7, 2024: MSphere
https://read.qxmd.com/read/38712943/bacterial-metallothionein-pmta-a-novel-stress-protein-found-on-the-bacterial-surface-of-pseudomonas-aeruginosa-and-involved-in-management-of-oxidative-stress-and-phagocytosis
#20
JOURNAL ARTICLE
Michele Maltz-Matyschsyk, Clare K Melchiorre, David A Knecht, Michael A Lynes
UNLABELLED: Metallothioneins (MTs) are small cysteine-rich proteins that play important roles in homeostasis and protection against heavy metal toxicity and oxidative stress. The opportunistic pathogen, Pseudomonas aeruginosa, expresses a bacterial MT known as PmtA. Utilizing genetically modified P. aeruginosa PAO1 strains (a human clinical wound isolate), we show that inducing pmtA increases levels of pyocyanin and biofilm compared to other PAO1 isogenic strains, supporting previous results that pmtA is important for pyocyanin and biofilm production...
May 7, 2024: MSphere
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