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Iranian Journal of Veterinary Research

V K Singh, A Kumar, R P Pandey, S K Yadav
Skin affections in canines are common and their treatment depends upon the pathogens involved. Delayed or no response to traditional treatment indicates the involvement of unusual etiology or the multidrug resistance. This report describes the diagnosis and therapeutic management of a rare case of phaeohyphomycosis caused by Alternaria alternata complicated with Pseudomonas aeruginosa associated pyoderma in a Doberman pinscher dog. Identification of the etiological agents was based on microscopy and culture of the skin lesions and further confirmation by polymerase chain reaction (PCR) amplification...
2018: Iranian Journal of Veterinary Research
N Kushwaha, A Mohan, A U Bhikane
Acetamiprid is a first generation systemic neonicotinoid insecticide, routinely used for crop protection against sucking type insects. It is likely to be of low toxicity in mammals but severe poisoning may occur if ingested in large amount. A case of buffalo with accidental ingestion of acetamiprid was presented with severe gastrointestinal symptoms and respiratory distress. The patient was managed successfully with symptomatic and supportive treatment. As far as the present report is concerned, it is the first report of acute acetamiprid poisoning in buffalo from India...
2018: Iranian Journal of Veterinary Research
M R Rezvani, N Sayadpour, F Saemi
The present study was aimed at studying the effects of inclusion of pomegranate seed extract to fat-containing diets on nutrients digestibility, intestinal microflora and performance of broilers. A total of 320 one-day-old broiler chicks (Ross 308) were selected in a 2 × 2 × 2 factorial experiment in a completely randomized design with 4 replicates of 10 birds of each. The dietary treatments consisted of pomegranate seed extract (0 and 2%), commercial Nutriad® antioxidant (0 and 0.01%) and soybean oil without antioxidant (0 and 6% during the grower period and 0 and 8% for finisher period)...
2018: Iranian Journal of Veterinary Research
M Priyanka, K Jeyaraja, P S Thirunavakkarasu
Present study was conducted with the objectives of determining the renal vascular resistance in dogs with diabetes mellitus and to study the correlation between the indices of renovascular resistance with glycemic status, systolic blood pressure (SBP) and proteinuria in dogs with diabetes mellitus. This study was conducted on seventeen diabetic dogs and ten apparently healthy dogs. Increased renal resistive index (RI) and pulsatility index (PI) were observed in diabetic dogs as compared to healthy dogs. Systemic hypertension and proteinuria were observed in 10 and 3 out of 17 diabetic dogs, respectively...
2018: Iranian Journal of Veterinary Research
M Pajokh, F Mesbah, H Bordbar, T Talaei-Khozani
Follicular atresia is a phenomenon that leads to evacuation of the ovary from the oocytes and the occurrence of menopause. The contribution of various types of cell death in atresia at different follicular developmental stages requires extensive investigation. In this study, we evaluated 3 types of programmed cell death (PCD), apoptosis, autophagy, and necrosis, in juvenile mouse ovary when we can observe all follicular stages as well as atresia. Ovaries from juvenile mice on the 21st post-natal (PN) day were prepared histologically for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to evaluate apoptosis and immunohistochemistry for beclin-1 to evaluate the autophagy marker...
2018: Iranian Journal of Veterinary Research
T Ilić, P Stepanović, K Nenadović, S Dimitrijević
A study was made on the prevalence of some parasitic infections appearing in domestic rabbits obtained from individual breeders in Serbia in order to improve the agricultural production of these animals. Aside from economic reasons (meat production and reproduction) rabbits are bred for the research purposes, and races are kept as household pets. For these reasons, among others, it is important to gain knowledge of medical culprits including causes of parasitic diseases that compromise their health, well-being and cause economic losses...
2018: Iranian Journal of Veterinary Research
A Baranwal, A Sonwane, A Chauhan, M Panigrahi, A K Sharma
The present study was conducted to quantify and compare TLR2 (toll-like receptor 2) activity in monocyte-derived macrophages of zebu (Tharparkar) and crossbred (Holstein-Friesian × Jersey × Brown Swiss × Hariana) cattle. The cells were either induced with Pam3CSK4 or kept as control. The TLR2 activity was quantified in terms of IκB-α inhibitory subunit (NFKBIA) messenger RNA (mRNA) copies using real-time, one-step reverse transcription-polymerase chain reaction (RT-PCR). Toll-like receptor 2 activity of induced cells was in the range of 1060421 ± 477937 (n=3) to 3514715 ± 290222 (n=3) copies for Tharparkar cattle (n=7) and in the range of 1365532 ± 47243 (n=3) to 3016510 ± 172340 (n=3) copies for the crossbred cattle (n=7)...
2018: Iranian Journal of Veterinary Research
F Fatahnia, A Azarfar, S Menatian, Sh Joz Ghasemi, A Poormalekshahi, A N Shokri
The aim of this study was to investigate the effect of diets containing roasted soybean (R), extruded soybean (E) or their combination (RE) on dairy cow performance and milk fatty acid (FA) profile. Nine multiparous lactating Holstein cows (680 ± 25 kg BW; 90 ± 10 DIM; means ± SD) were randomly assigned to a triple 3 × 3 Latin square design. Dry matter intake (DMI), apparent nutrient digestibility, milk yield, composition and FA profile and efficiency of nitrogen (N) utilization for milk production were measured...
2018: Iranian Journal of Veterinary Research
M M M Kandiel, A R M El Khawagah
Egypt is anticipated to be potentially influenced by the global climate warming. Therefore, the current study aimed to evaluate the influence of age on the fertility potential of Arabian stallions during summer breeding months. Arabian horses grouped according to their age into three groups, each involved six stallions: young (5-6 years), middle (11-12 years) and old (15-20 years) age groups, were weekly sampled during the months of July-August. Ejaculates were collected using artificial vagina, Missouri model, and examined for pH, volume, concentration, motility, livability and morphological abnormalities...
2018: Iranian Journal of Veterinary Research
S Mostoufi-Afshar, M Tabatabaei, M M Ghahramani Seno
Mycobacterium avium subspecies paratuberculosi s (MAP), as an obligate intracellular bacterium, causes paratuberculosis (Johne's disease) in ruminants. Plus, MAP has consistently been isolated from Crohn's disease (CD) lesions in humans; a notion implying possible direct causative effect for MAP in CD development. Infections caused by MAP are refractory to treatment and in many cases the treatment does not easily resolve the infection. Studying the molecular mechanisms of host-pathogen interaction is helpful in identifying possible drug targets...
2018: Iranian Journal of Veterinary Research
T Karaca, S Demirtas, D Uzun Goren
Pendrin (PDS) and sodium/iodide symporter (NIS) are transmembrane proteins that are located in numerous tissue types, particularly thyroid follicular epithelial cells, where they are entrusted with the regulation of iodine molecules. In the present study, we aimed to clarify changes in PDS and NIS protein expression, in the testicular tissue of prepubertal and post pubertal rats at normal or diabetic conditions. Forty Wistar albino male rats (20 prepubertal and 20 post pubertal) were divided into four groups, as follows: group I was prepubertal control, group II was prepubertal diabetic (60 mg/kg intraperitoneal [ip] streptozotocin [STZ]), group III was post pubertal control, and group IV was post pubertal diabetic (60 mg/kg ip STZ)...
2018: Iranian Journal of Veterinary Research
A Meimandi-Parizi, A Oryan, A Bigham-Sadegh, E Sayahi
The aim of this study was to compare the efficacy of honey bee venom (BV) and royal jelly (RJ) alongside chitosan scaffold (CS) in improving radius bone defect in rats. A total of 60 full thickness radial bone defects with a length of 5 mm were created in 60 male Wistar rats. Six healthy radial bones (3 rats) were also assigned as normal control for biomechanical studies. The defects were left empty (untreated group) or were filled by the autograft (autograft group), CS (CS group), CS alongside the BV solution (CS-BV group), and CS alongside the RJ solution (CS-RJ group)...
2018: Iranian Journal of Veterinary Research
S Mahajan, S Dey, A Kumar, P N Panigrahi
This is a prospective, controlled, randomized clinical study to evidence the role of nitrosative stress in development of overt neurological sign of canine distemper (CD). The enrollment of cases wasa made on basis of overt sign of CD (n=139) and the findings were compared with apparently healthy dogs (n=15). The CD specific immunoglobulins resulted in 94 confirmed positive cases. The nitric oxide (NO) and neuronal nitric oxide synthase (nNOS) concentration in cerebrospinal fluid (CSF) (18.08 ± 2.76, 415.84 ± 46...
2018: Iranian Journal of Veterinary Research
M Nemati, S Menatian, Sh Joz Ghasemi, R Hooshmandfar, M Taheri, T Saifi
The present study was conducted to study the effect of protected-glutamine (Gln) supplementation on dry matter intake (DMI), milk yield (MY) and composition, somatic cell counts (SCC) and blood parameters in fresh cows. Forty Holstein cows at zero day of parturition (calving day = day 0) were divided into four groups (n=10), and fed ( ad libitum ) with one of the diets including: basal diet (control), basal diet supplemented with 150 (low Gln, LG), 250 (medium Gln, MG) or 350 (high Gln, HG) g of Gln protected with formaldehyde/cow per day...
2018: Iranian Journal of Veterinary Research
S Mishra, S K Sahu, S Panigrahi, S S Biswal, S R Mishra, R Ranjan, D N Mohanty, B Pattnaik, S Das
This study compared the therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination and prostaglandin F2α (PGF2α) in longstanding cases of endometritis and evaluated their impact on Interleukin-6 ( IL-6 ) and Interleukin-10 ( IL-10 ) transcript level in peripheral blood leukocytes. Eighteen endometritic crossbred Jersey cows were randomly allotted to three groups (six in each) viz. Group I (levofloxacin combo treatment I/U), group II (PGF2α treatment I/M), group III (no treatment, control), and group IV (six non-endometritic healthy cyclic) was taken for comparison study...
2018: Iranian Journal of Veterinary Research
A H Ahmed, I M Maulood
Urotensin-II (U-II), the most potent vasoconstrictor that has recently been recognized as a new candidate in cardiovascular dysfunction, might exert vasoconstriction through, at least partially, potassium channels that are predominant in both endothelial and vascular smooth muscle cells (VSMCs). The present study was designed to evaluate the roles of potassium channels in vascular responses to U-II in intact and mercury induced endothelial dysfunction in rat aorta. The study involved pre-incubation of rat aortic rings with potassium channels blockers: charybdotoxin (chtx), tetraethylammonium (TEA), barium chloride (BaCl2 ), glibenclamide, 4-aminopyridine (4-AP) and clotrimazole...
2018: Iranian Journal of Veterinary Research
M M Elmaghraby, A F El-Nahas, M M Fathala, F M Sahwan, M A Tag El-Dien
Mastitis is a costly disease of dairy cattle as it causes a loss in milk yield and milk quality in affected cows. Toll-like receptor ( TLR ) genes play a role in the host response to a variety of organisms including those inducing mastitis. In the present study, we investigated the polymorphism of TLR2 , 4 , 6 and 9 genes in Holstein cattle and their possible association with clinical mastitis (CM), milk somatic cell scores (SCS) and milk production traits. From a large commercial Holstein herd, thirty-eight blood samples were collected; 19 from cows without a previous lifetime history of mastitis (non-susceptible), and 19 from Holstein cows with at least three previous episodes of mastitis (susceptible)...
2018: Iranian Journal of Veterinary Research
A Magouz, Sh Medhat, S Abou Asa, A Desouky
Infectious laryngotracheitis (ILT) disease is an acute highly contagious viral disease leading to massive economic losses to the national poultry industry. This study aimed to identify the most accurate and rapid diagnostic methods to rescue layer poultry farms from intense outbreaks in Egypt. Fifty pathological specimens were collected and subjected to virus isolation (VI), histopathology, direct fluorescent antibody technique (FAT) and polymerase chain reaction (PCR). Egg inoculation revealed stunted growth and white pock lesions on chorioallantoic membranes (CAM) in 23 samples...
2018: Iranian Journal of Veterinary Research
S Datta, P Dandapat, R Jas
An indirect-ELISA for the diagnosis of mixed gastrointestinal (GI) nematode infection comprising Oesophagostomum , Haemonchus and Trichuris species was standardized using crude somatic antigen of Oesophagostomum columbianum (CSAg-Oc) and sera of slaughtered goats with known parasitological status including Oesophagostomum , Haemonchus , and Trichuris (strong positive), Haemonchus and Trichuris (weak positive) and parasite free goats (negative). Two cut-off points, i.e. higher and lower cut-off were determined using the strong positive, weak positive and the negative control sera of goats...
2018: Iranian Journal of Veterinary Research
N Zareifard, A Soleimani, T Talaei-Khozani, S Bahmanpour
Alginate, a non-toxic polysaccharide isolated from brown algae, is a widely used 3-dimensional (3D) porous scaffold for the granulosa cell and follicle encapsulation. However, impurities in commercial alginate can lead to alginate biocompatibility reduction. The aim of this study was to evaluate in vitro behavior of the granulosa cells seeded on the purified alginate in varying concentrations compared with matched non-purified ones. We produced a purified alginate using a simple and efficient method. Then, the granulosa cells from mice were isolated and seeded in various concentrations of (0...
2018: Iranian Journal of Veterinary Research
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