Read by QxMD icon Read

Future Science OA

Frøydis Sved Skottvoll, Henriette Engen Berg, Kamilla Bjørseth, Kaja Lund, Norbert Roos, Sara Bekhradnia, Bernd Thiede, Cecilie Sandberg, Einar Osland Vik-Mo, Hanne Roberg-Larsen, Bo Nyström, Elsa Lundanes, Steven Ray Wilson
Aim: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. Materials & methods: 'Case study' exosomes isolated from patient-derived cells from glioblastoma multiforme and a breast cancer cell line were analyzed. Results: Transmission electron microscopy, dynamic light scattering, western blotting, and so forth, revealed comparable performance. Potential protein biomarkers for both diseases were also identified in the isolates using nanoLC-MS...
January 2019: Future Science OA
Francesca Lake
No abstract text is available yet for this article.
January 2019: Future Science OA
Juliana Miranda de Lucena Valim, Fernanda Gomes Gonçalves Chaer, Fernanda D'Oliveira Guimarães da Silveira, Verônica Palmiro da Silva E Lima, Branca Dias Batista de Souza
Aim: To assess drug switching, rates of remission and disease activity in Brazilian patients with rheumatoid arthritis (RA) treated with biologic agents. Materials & methods: Using a retrospective method, a total of 94 adult patients were included. Results: Anti-TNF was the first choice therapy in 85 (90.4%) patients. After an average of 8 years of follow-up, 55 (59%) patients were taking anti-TNF, 18 (19%) abatacept, eight (9%) tocilizumab and 13 (14%) rituximab...
January 2019: Future Science OA
Gerry Amor Camer, Yuki Oikawa, Hitomi Omaki, Daiji Endoh
Aim: To produce synthetic nucleotides of notifiable dengue virus (1-4 types), Japanese encephalitis, yellow fever and Zika flaviviruses. These notifiable flaviviruses, particularly dengue and Zika, are problematic mosquito-borne infections in the Philippines, as well as in those countries with tropical and subtropical climates. Method: An algorithmic design formulation of overlap extension - polymerase chain reaction (OE-PCR) was performed to propagate 50-60 oligomer lengths of select notifiable flaviviral RNAs to DNA nucleotides via the two-step process of OE-PCR...
January 2019: Future Science OA
Christina A Sutherland, Can Ozbal, David P Nicolau
Aim: We describe the validation of an HPLC-MS/MS method to analyze ceftolozane and tazobactam simultaneously in saline matrixes. Materials & methods: An Agilent 1260 HPLC interfaced to an Agilent 6470 triple-quadrupole mass spectrometer was used for quantification. A reverse-phase column running a gradient of water and acetonitrile containing 0.1% formic acid mobile phase at a flow rate of 1.0 ml/min provided chromatographic fractionation. Tazobactam15 N3 was used as the internal standard...
January 2019: Future Science OA
Hitesh Shukla, Jessica Louise Mason, Abdullah Sabyah
The development of cardiovascular diseases (CVDs) is due to a complex interaction between the genome and the environment. Understanding how genetic differences in individuals contribute to their susceptibility to CVDs can help guide practitioners to give the best advice to achieve a favorable outcome for the patient. As genome technologies evolve, genotyping of individuals could be available to all patients using a simple saliva test. Large-scale genome-wide association studies and meta analyses have provided powerful insights into polymorphisms that may be predictive of disease and an individual's response to certain nutrients, but moving forward it is imperative that these insights can be applied in the medical setting to reduce the incidence and mortality of CVDs...
January 2019: Future Science OA
Adarsh Gandhi, Murali Matta, Tesfalem Zere, James Weaver
Although a marked decrease in mortality associated with bacterial infections is attributed to the discovery of antibiotics, antibiotic resistance has become a global health concern due to their misuse. A dynamic in vitro hollow-fiber system was used to study antibiotic resistance in Escherichia coli using ampicillin. An LC-MS/MS assay was validated for quantitative analysis of ampicillin in Luria-Bertani broth. The assay was linear from 0.10-50.00 μg/ml. The assay met acceptance criteria for inter- and intra-assay precisions and accuracies across three quality controls...
January 2019: Future Science OA
Wen Chen, Yoon Y Hwang, Jeremy W Gleaton, James K Titus, Nicholas J Hamlin
We optimized a peptide extraction and LC-MS protocol for identification and quantification of antimicrobial peptides (AMPs) in biological samples. Amphipathic AMPs were extracted with various concentrations of ethanol, methanol, acetonitrile, formic acid, acetic acid or trichloroacetic acid in water. Yields were significantly greater for extraction with 66.7% ethanol than other extraction methods. Liquid chromatography was accomplished on a C18 column with a linear gradient of acetonitrile-formic acid, and mass spectrometry detection was performed in the positive electrospray multiple reaction monitoring mode by monitoring the transitions at m/z 385...
January 2019: Future Science OA
Francesco Perri, Franco Ionna, Paolo Muto, Massimiliano Di Marzo, Francesco Caponigro, Francesco Longo, Giuseppina Della Vittoria Scarpati, Giuseppe Di Lorenzo, Mario Giuliano, Raffaele Solla
Squamous cell carcinoma of the head and neck (SCCHN) accounts for 5-7% of all malignancies. About 60% of newly diagnosed SCCHN are detected as locally advanced disease. Chemoradiation is a standard option and response rate to it is variable. Recently, a genetic classification of SCCHN has been proposed by Chung et al ., who categorized all SCCHN into four subtypes. The basal-like variant is characterized by high expression of EGFR. Literature data suggest higher efficacy of accelerated and/or hyperfractionated radiotherapy, if compared with conventional radiotherapy in the subgroup of patients with high EGFR expression...
January 2019: Future Science OA
Vladimir N Pak
No abstract text is available yet for this article.
January 2019: Future Science OA
Lamiaa Ma Ali, Pasquina Marzola, Elena Nicolato, Silvia Fiorini, Marcelo de Las Heras Guillamón, Rafael Piñol, Lierni Gabilondo, Angel Millán, Fernando Palacio
Aim: To study the efficiency of multifunctional polymer-based superparamagnetic iron oxide nanoparticles (bioferrofluids) as a T2 magnetic resonance contrast agent and their uptake and toxicity in liver. Materials & methods: Mice were intravenously injected with bioferrofluids and Endorem® . The magnetic resonance efficiency, uptake and in vivo toxicity were investigated by means of magnetic resonance imaging (MRI) and histological techniques. Results: Bioferrofluids are a good T2 contrast agent with a higher r2 /r1 ratio than Endorem...
January 2019: Future Science OA
Emmanuel Seront, Gaëtan Catala, Alexandre Dermine, Sarah Lejeune, Stephane Rysselinck
Metastatic urothelial cancer is an aggressive disease associated with a poor prognosis. In the first-line setting, platinum-based chemotherapy is the standard of care but resistance rapidly occurs. After failure of platinum-based therapy and in cisplatin-ineligible patients, therapeutic options are limited. Malignant cells evolve mechanisms to evade immune recognition, including the expression of cell-surface molecules, named immune checkpoints, on tumor and tumor-specific lymphocytes. Immunotherapy, by targeting these checkpoints, represents a new tool to improve the patient outcome in advanced urothelial carcinoma (UC)...
December 2018: Future Science OA
Frances Verter, Pedro Silva Couto, Alexey Bersenev
No abstract text is available yet for this article.
December 2018: Future Science OA
Marian Manciu, Sorour Hosseini, Teresa Di Desidero, Giacomo Allegrini, Alfredo Falcone, Guido Bocci, Robert A Kirken, Giulio Francia
Aim: To construct classification scores based on a combination of cancer patient plasma biomarker levels, for predicting progression-free survival. Methods: The approach is based on the optimization of the biomarker cut-off values, which maximize the statistical differences between the groups with values lower or larger than the cut-offs, respectively. An intuitive visualization of the quality of the classification score is also proposed. Results: Even if there are only weak correlations between individual biomarker levels and progression-free survival, scores based on suitably chosen combination of three biomarkers have classification power comparable with the Response Evaluation Criteria in Solid Tumors criteria classification of response to treatments in solid tumors...
December 2018: Future Science OA
Andrew W Maksymiuk, Daniel S Sitar, Rashid Ahmed, Brian Cheng, Horacio Bach, Rushita Adhikari Bagchi, Nina Aroutiounova, Paramjit S Tappia, Bram Ramjiawan
Aim: SSAT-1 is an enzyme that plays a critical role in cell growth. Amantadine, a FDA-approved antiviral drug, is a substrate for SSAT-1. The utility of amantadine as an agent to demonstrate elevated SSAT-1 activity linked to cancer was conducted. Results: High levels of SSAT-1 expression were measured in tumor human cell lines, and in breast, prostate and lung tumor tissue. An increase in the urinary levels of acetylated amantadine in cancer patients was observed...
December 2018: Future Science OA
Ziad Bakouny, Elie El Rassy, Fares Yared, Antoine Abi Lutfallah, Marwan Ghosn, Fadi Farhat, Joseph Kattan
Aim: The rationale for platelet-to-lymphocyte ratio (PLR) in chronic lymphocytic leukemia (CLL) is that both the platelet and lymphocyte counts are affected by the CLL pathogenesis and could influence treatment decision-making. Methods: Demographic and clinical data of CLL patients diagnosed at our institution between 1989 and 2013 were collected. Cox regression models were used to evaluate the role of PLR in the duration of watchful waiting, postdiagnosis survival and postchemotherapy survival...
December 2018: Future Science OA
Margaux Jegaden, Anne-Déborah Bouhnik, Marie Préau, Marc-Karim Bendiane, Patrick Peretti-Watel, Julien Mancini, Blandine Courbiere
Aim: To report fertility status perception, fertility preservation and desire to have children in French cancer survivors 2 and 5 years after diagnosis. Methods: A total of 427 women and 115 men self-reported treatment-induced infertility, fertility status, access to gamete conservation, desire to have children and pregnancy/live births. Results: A total of 96.5% of men and 92.9% of women were thought to be fertile at diagnosis and 38% desired to have children...
December 2018: Future Science OA
Heeresh Shetty, Adesh Kakade, Shishir Shetty, Prasanna Neelakantan, Saurabh Nagar, Rajiv S Desai, Kavita Beri
Aim: Dental pulp stem cells, which are primarily derived from the pulp tissues of human teeth, have rarely been obtained from natal teeth. This study investigated the stem cell and differentiation markers of the dental pulp of natal teeth using immunohistochemistry. Materials & Methods: The pulp tissue from extracted natal teeth (n = 2) of a 20-day-old healthy male was examined for immunohistochemical expression of stem cell (Oct-4 and SOX 2) and differentiation markers (Nestin, CD 44, desmin, osteopontin and Ki- 67)...
December 2018: Future Science OA
Chunlei Zhang, Jun Xiong, Qi Yang, Ye Wang, Haoqing Shi, Qinqin Tian, Hai Huang, Depei Kong, Jianmin Lv, Dan Liu, Xu Gao, Xiaoyuan Zi, Yinghao Sun
Aim: There is little knowledge about the expression profile and function of circular RNAs (circRNAs) in prostate cancer (PCa). Methods: The expression profiles of circRNAs in RWPE-1, 22RV1 and PC3 cells were explored via high-throughput circRNAs sequencing and validated by real-time qPCR. The roles of differentially expressed circRNAs were evaluated by bioinformatics analyses. Results: Altogether 9545 circRNAs were identified and hundreds of differentially expressed circRNAs were recognized...
October 2018: Future Science OA
Maria Virginia Villegas, Sue Lyon
Antibiotic resistance was a major topic of interest for the nearly 13,000 physicians, microbiologists and scientists who attended European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) 2018. Much discussion centered round the potential benefits of novel antibiotics that are either already approved or under investigation in the treatment of infections caused by resistant Gram-negative pathogens. There was also general acceptance of the importance of ensuring that antibiotic stewardship is implemented in every ward throughout every hospital to ensure that these novel drugs are used appropriately and to combat the development of resistance...
October 2018: Future Science OA
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"