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Molecular Biology Research Communications

Avneet Kaur, Vanita Vanita, JaiRup Singh
In India, mutations in Cytochrome P450 ( CYP1B1 ) are a predominant cause of not only primary congenital glaucoma (PCG) but also involved in primary open angle glaucoma (POAG) and juvenile onset glaucoma (JOAG). After ethical clearance, 100 POAG patients, 30 primary angle closure glaucoma (PACG) patients and 130 ethnically matched controls were recruited in this study. Genomic DNA was isolated from the blood and screened for p.Arg368His mutation in CYP1B1 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...
December 2018: Molecular Biology Research Communications
Habib Ghaznavi, Ali Asghar Kiani, Mohammad Soleiman Soltanpour
Coronary artery disease (CAD) is a common health problem with a high rate of disability and death. Dyslipidemia and altered metabolism of Apo-lipoproteins are involved in the CAD pathogenesis. The current study investigated two common polymorphisms (rs429358 and rs7412) and promoter DNA methylation status of APOE in the Iranian CAD patients and control subjects. Two hundred angiographically documented CAD patients and 200 control subjects were included in the study. The APOE polymorphism analysis was done by PCR-RFLP technique and DNA methylation status was evaluated by methylation specific PCR...
December 2018: Molecular Biology Research Communications
Zahra Hemati, Masoud Haghkhah, Abdollah Derakhshandeh, ShoorVir Singh, Kundan Kumar Chaubey
The aim of this study is to identify, clone and express a Mycobacterium avium subsp. paratuberculosis specific immunogenic antigen candidate, in order to develop better reagents for diagnosis and vaccines for the protection of the host. Therefore, MAP2191 gene (a member of MAP mce 5 operon) from MAP, was isolated and characterized by Bioinformatics tools and in vitro experiments. Then, a novel Mce-whole protein encoded by MAP2191 gene was amplified and sub-cloned into E. coli . We tried to express the Mce/whole protein in different condition along with a positive expression control (pET28a-Mce/truncated plasmid that we know express well), to ensure that nothing is wrong regarding culture/induction condition...
December 2018: Molecular Biology Research Communications
Shahrzad Haghir, Abbas Alemzadeh
Ethylene response factor proteins are important for regulating gene expression under different stresses. Different isoforms for ERF have previously isolated from bread wheat ( Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5 . We isolated, cloned and molecular characterized a novel one based on TdERF1 , an isoform in durum wheat ( Triticum turgidum L.) and called TaERF6 . Its cDNA was synthesized, sequenced and compared with genomic sequence to figure out intron and exon regions and determine coding sequence region...
December 2018: Molecular Biology Research Communications
Hossein Zeinalzadeh-Tabrizi, Kamil Haliloglu, Mehdi Ghaffari, Arash Hosseinpour
Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding field crops. Twenty-one microsatellite markers used to assess genetic diversity and relationship of 68 sunflower genotypes ( Helianthus annuus L.). All of 21 pairs of SSR (Simple Sequence Repeats) markers produced a total number of 49 polymorphic bands. DNA fragments ranged from 92 to 850 bp. The highest and lowest polymorphic information content (PIC) values were determined as 0.58 and 0.10 for marker Ha806-ar and Ha494-ar...
September 2018: Molecular Biology Research Communications
Leila Karami-Gadallo, Leila Ataie-Fashtami, Mahmood Ghoranneviss, Majid Pouladian, Dariush Sardari
Recently non-thermal plasma (NTP) is applied for many therapeutic applications. By NTP irradiating to the tissues or cell-lines, the water molecules (H2 O) would be also activated leading to generate hydrogen peroxide (H2 O2 ). By irradiating plasma to bio-solution, its main output including vacuum UV to UV causes the photolysis of H2 O leading to generate hydroxyl (OH) molecules in couple forms with ability to convert to H2 O2 . Additionally, other plasma's output the oxygen atoms could also penetrate under the liquid's surface and react with H2 O to generate H2 O2 ...
September 2018: Molecular Biology Research Communications
Arezoo Cheraghi, Fatemeh Rahmani, Abdollah Hassanzadeh-Ghorttapeh
This study describes the genetic relationships among 34 varieties of Lallemantia iberica using inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP). Samples were collected from Agriculture Research Center of Urmia city (northwest Iran). Ten IRAP and REMAP primers generated 76 scorable electrophoretic bands with 78.94% polymorphism. The pair-wise Jacquard genetic similarity varied from 0.48 to 0.94 for IRAP and REMAP data combined. Average PIC values for IRAP and REMAP markers were 0...
September 2018: Molecular Biology Research Communications
Abdolkarim Moazeni-Roodi, Mohammad Hashemi
Several studies examined the relationship between STAT3 rs1053004 polymorphism and the risk of some human cancers, but the findings remains inconclusive. To evaluate the impact of STAT3 rs1053004 on cancer risk, we conducted a meta-analysis of all available studies including 4,605 cancer cases and 5,248 controls. Eligible studies were identified by searching PubMed, Web of Science, Scopus, and Google scholar databases. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated in codominant, dominant, recessive, overdominant, and allele models to quantitatively estimate the association...
September 2018: Molecular Biology Research Communications
Sandeep Saini, Chander Jyoti Thakur, Varinder Kumar, Suchita Tandon, Varuni Bhardwaj, Sonia Maggar, Stanzin Namgyal, Gurpreet Kaur
Current re-emergence of Nipah virus (NiV) in India caused 11 deaths so far and many patients were kept in quarantine. A thorough study of previous outbreaks occurred in Malaysia, Bangladesh and India represents cases with high rate of fatality due to acute encephalitis. Our work involves genome analysis of NiV for prediction of miRNAs and their targeted genes in human in order to understand encephalitis origin. Ab-intio program-VMir was used for initial screening of genome, obtained nine pre-miRNAs was analyzed by ViralMir to check for any pseudo pre-miRNAs...
September 2018: Molecular Biology Research Communications
Shivangi Paliwal, Robert Wheeler, Tom D Wolkow
The DNA structure checkpoint protein Rad26ATRIP is also required for an interphase microtubule damage response. This checkpoint delays spindle pole body separation and entry into mitosis following treatment of cells with microtubule poisons. This checkpoint requires cytoplasmic Rad26ATRIP , which is compromised by the rad26:4A allele that inhibits cytoplasmic accumulation of Rad26ATRIP following microtubule damage. The rad26::4a allele also disrupts minichromosome stability and cellular morphology, suggesting that the interphase microtubule damage checkpoint pathway operates in an effort to maintain chromosome stability and proper cell shape...
September 2018: Molecular Biology Research Communications
Mahsa Salehi, Mohammadreza Sharifi
Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell growth, proliferation, differentiation, apoptosis and migration are regulated by lncRNAs. In various cancers, aberrant expression of lncRNAs has been reported...
June 2018: Molecular Biology Research Communications
Roya Zanganeh, Rashid Jamei, Fatemeh Rahmani
Heavy metals pollution is one of the key environmental problems. In this research, the effect of seed priming with salicylic acid and sodium hydrosulfide was investigated on methionine and arginine amino acids contents and some compounds derived from their metabolism as well as ZmACS6 and ZmSAMD transcripts levels in maize plants under lead stress. For this purpose, maize seeds were soaked in salicylic acid (0.5 mM) and sodium hydrosulfide (0.5 mM) for 12 hours and then exposed to lead (2.5 mM) for 9 days. The results showed that lead stress reduced nitric oxide content and shoot ZmACS6 and ZmSAMD transcript levels while increased glycine betaine, methionine, arginine and proline amino acids contents as well as root ZmACS6 and ZmSAMD transcript levels...
June 2018: Molecular Biology Research Communications
Hussain Mubbashir, Shahzad Munir, Rahim Kashif, Haider Bashir Nawaz, Basit Abdul, Khattak Baharullah
Dengue is a vector-borne disease caused by dengue virus. According to the recent report of CDC that one-third population of the world are at high risk with Dengue fever. The prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. Aedes mosquitoes was reported as the main cause of transmission of dengue virus. So the current study was planned to characterize the virus in Aedes mosquitoes collected from different area of Pakistan. In current investigation, Aedes mosquitoes and larvae were trapped under conducive conditions which are counted as 495 Aedes mosquitoes and 260 Aedes larvae...
June 2018: Molecular Biology Research Communications
Sandeep Saini, Chander Jyoti-Thakur, Varinder Kumar, Akshay Suhag, Niharika Jakhar
Interleukin-4 (IL-4) is a multifunctional cytokine that plays a critical role in apoptosis, differentiation and proliferation. The intensity of IL4 response depends upon binding to its receptor, IL-4R. The therapeutic efficiency of interleukins can be increased by generating structural mutants having greater stability. In the present work, attempts were made to increase the stability of human IL-4 using in-silico site directed mutagenesis. Different orthologous sequences of IL4 from Pan troglodytes , Aotusnigriceps , Macacamulatta , Papiohamadryas , Chlorocebusaethiops , Vicugnapacos , Susscrofa and Homo sapiens were aligned using Clustal Omega that revealed the conserved and non-conserved positions...
June 2018: Molecular Biology Research Communications
Habib Ghaznavi, Khalil Mahmoodi, Mohammad Soleiman Soltanpour
Coronary artery disease (CAD) is a common health problem in Iranian population. ATP binding cassette transporter A1 (ABCA1) plays central role in the efflux of the cholesterol from peripheral tissues back to liver. Inactivation of ABCA1 by epigenetic change such as DNA methylation may contribute to the development of CAD. The present study investigated the association between promoter DNA methylation status of ABCA1 with the development and severity of CAD. Our study population consisted of 110 angiographically documented CAD patients and 110 controls...
June 2018: Molecular Biology Research Communications
Olukanni A Titilayo, Amoo O Samuel, Olukanni O David, Taiwo I Adewunmi
Genetically, every individual is unique; this may stem from inheritance, geographical locations, and/or environmental interactions. This study examined the possibility of developing a cheap and easy-to-use marker that can distinguish among the three ethnic groups in Nigeria using RAPD-PCR. Five RAPD primers, OPA1-3 and OPC1-2, were randomly selected and used to amplify DNA samples isolated from blood of eighteen human subjects representing the three major ethnic groups in Nigeria (six subjects each). Genomic DNAs were extracted using DNA isolation kit, RAPD-PCR amplification was performed and gel electrophoresis was done...
June 2018: Molecular Biology Research Communications
Aminallah Tahmasebi, Alireza Afsharifar, Ahmad Heydari, Mohammad Mehrabadi
The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysis of 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K coding regions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotypes were detected with haplotype diversity ranging from 0.8 to 1.0 and nucleotide diversity from 7.58 to 13.62. The positions 33 and 30 indicated the highly positive and negative selection (with the highest and the lowest dN-dS values), respectively...
March 2018: Molecular Biology Research Communications
Samira Ebrahimi, Hassan Mohabatkar
Streptococcal bacteria are among dangerous human pathogens with major prevalence worldwide. A good vaccine against streptococcal bacteria should have epitopes that confer protection from infection by different streptococcal bacteria types. we aimed was to recognize the most immunogenic and conserved epitopes of streptococcal bacteria, which could be a potential candidate for vaccine development. Nineteen different M proteins of different streptococcal bacteria were chosen and analyzed. Nine-mer epitopes able to simulate both cells mediate and humoral immunity were predicted...
March 2018: Molecular Biology Research Communications
Mohammadreza Sharifi, Mahdi Fasihi-Ramandi, Abdolkarim Sheikhi, Abbas Moridnia, Maryam Saneipour
MicroRNAs (miRNAs) involved in regulation of the genes. The CCAAT/enhancer-binding protein-α ( CEBPα ) is a crucial transcription factor for normal hematopoiesis and cell cycle that frequently disrupted in human acute myeloid leukemia (AML). The miR-182 up-regulation in several malignant diseases such as AML was reported, in the other hand bioinformatics analysis revealed CEBPα targeted by miR-182.miR-182-5p inhibition in human acute promyelocytic leukemia (APL) cell line was performed by using locked nucleic acid (LNA) and subsequently miR-182-5p and CEBPα expression, apoptosis, necrosis and cell proliferation were measured...
March 2018: Molecular Biology Research Communications
Abbas Seidi, Sina Mirzaahmadi, Khalil Mahmoodi, Mohammad Soleiman-Soltanpour
Coronary artery disease (CAD) is considered as a chronic inflammatory disease initiated from early childhood. Nuclear factor κB (NF κB) and κB1A (NF κB1A) are the key regulators of inflammatory responses. The NFKB1 -94ATTG ins/del and NFKB1A -826C/T polymorphisms may contribute to the development of CAD. The aim of the present study was to investigate the association of these polymorphisms with the risk of CAD. The study population included 120 patients with angiographically confirmed CAD and 100 matched controls...
March 2018: Molecular Biology Research Communications
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