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Iranian Journal of Biotechnology

Mohammad Sadegh Sabet, Katayoun Zamani, Tahmineh Lohrasebi, Mohammad Ali Malboobi, Mostafa Valizadeh
Background: Overexpression of known genes encoding key phosphate (Pi)-metabolizing enzymes, such as acid phosphatases (APases), is presumed to help plants with Pi availability and absorption as they are mostly exposed to suboptimal environmental conditions for this vital element. Objectives: In this study, the overexpression effect of AtPAP26 , one of the main contributors in retrieving Pi from intracellular and extracellular compounds, was evaluated from various viewes in tobacco plant...
April 2018: Iranian Journal of Biotechnology
Maryam Monazzah, Zohreh Rabiei, Sattar Tahmasebi Enferadi
Background: One of the main sunflower diseases is the white mold Sclerotinia sclerotiorum . The oxalic acid (OA), which is one of the main pathogenicity factors of this fungus, beside the direct toxicity on the host, has other functions such as the disruption of the cell wall and chelating out the calcium ions. Objectives: Regarding the importance of this disease, it is important to study the reactions of the plant against OA which is a nonspecific toxin of many other necrotrophic fungi...
April 2018: Iranian Journal of Biotechnology
Uchenna Oyeagu, Charles O Nwuche, Christiana N Ogbonna, James C Ogbonna
Background: Although advantages of immobilization of cells through entrapment in calcium alginate gel beads have already been demonstrated, nevertheless, instability of the beads and the mass transfer limitations remain as the major challenges. Objective: The objective of the present study was to increase the stability, porosity (reduce mass transfer limitation), and cell immobilization capacity of calcium alginate gel beads. Materials and Methods: Sodium alginate was mixed with various concentrations of the starch or sugar and gelled in 2% calcium chloride solution...
April 2018: Iranian Journal of Biotechnology
Amir Mashayekhi, Shirin Shahbazi, Mirdavood Omrani
Background: Coagulation Factor VII is a vitamin K-dependent serine protease which has a pivotal role in the initiation of the coagulation cascade. The congenital Factor VII deficiency is a recessive hemorrhagic disorder that occurs due to mutations of F7 gene. In the present study C91S (p.C91S) substitution was detected in a patient with FVII deficiency. This mutation has not been characterized by a functional study. Objectives: In this study, we aimed to evaluate the impact of C91S substitution on factor VII expression and function...
April 2018: Iranian Journal of Biotechnology
Mona Shafaghi, Somayeh Maktoobian, Rahimeh Rasouli, Nader Howaizi, Hamideh Ofoghi, Parastoo Ehsani
Background: Rabies virus is a neurotropic virus that causes fatal, but, a preventable disease in mammals. Administration of rabies immunoglobulin (RIG) is essential for the post-exposure of the prophylaxis to prevent the disease. However, replacement of polyclonal RIGs with alternative monoclonal antibodies (MAbs) that are capable of neutralizing rabies virus has been recommended. Objectives: Here, we have investigated the transient expression of the full-size human MAb against rabies virus glycoprotein; the MAb SO57 in the tobacco plants using vacuum agro-infiltration...
April 2018: Iranian Journal of Biotechnology
Huimin Jia, Hedan Li, Lirong Zhang, Daqing Xu
Background: Corynebacterium glutamicum ( C. glutamicum ) is a potential host for the secretory production of the heterologous proteins. However, to this date few secretion-type gene expression systems in C. glutamicum have been developed, which limit applications of C. glutamicum in a secretory production of the heterologous proteins. Objectives: In this study, a novel and efficient general secretory (Sec) pathway-dependent type gene expression system for the production of heterologous proteins was developed in C...
April 2018: Iranian Journal of Biotechnology
Tahereh Ghashghaei, Mohammad Reza Soudi, Saman Hoseinkhani, Morteza Shiri
Background: Xanthomonas campestris is a biopolymer producing gram negative bacterium. Production of xanthan biopolymer can be affected by different extrinsic factors as well as surfactants. Hitherto, effects of nonionic surfactants on xanthan production have been studied in a limited number of articles. Objective: In the present study, nonionic surfactants were used to pursue their effects on improvement of xanthan production. Moreover, a number of cellular consequences upon the treatment were investigated with impacts on gum production...
April 2018: Iranian Journal of Biotechnology
Farzaneh Barkhordari, Mozhgan Raigani, Yeganeh Talebkhan Garoosi, Fereidoun Mahboudi, Fatemeh Davami
Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins. Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity, was investigated in EnBase system and the effect of low temperature cultivation on the increase of α-Luffin solubility was determined ...
April 2018: Iranian Journal of Biotechnology
Ryad Amdoun, Lakhdar Khelifi, Majda Khelifi-Slaoui, Samia Amroune, Mark Asch, Corinne Assaf-Ducrocq, Eric Gontier
Background: The use of the desirability function approach combined with the response surface methodology (RSM), also called Desirability Optimization Methodology (DOM), has been successfully applied to solve medical, chemical, and technological questions. It is particularly efficient for the determination of the optimal conditions in natural or industrial processes involving different factors leading to the antagonist responses. Objectives: Surprisingly, DOM has never been applied to the research programs devoted to the study of plant responses to the complex environmental changes, and thus to biotechnological questions...
April 2018: Iranian Journal of Biotechnology
Lamia Medouni-Haroune, Farid Zaidi, Sonia Medouni-Adrar, Sevastianos Roussos, Samia Azzouz, Véronique Desseaux, Mouloud Kecha
BACKGROUND: Olive pomace, as the main by-product of the olive oil industry, is recently recycled as fermentation substrate for enzyme production. OBJECTIVES: Actinobacteria isolates were separated from an Algerian soil under olive pomace cultivation and were evaluated for their lignocellulolytic enzymes production. MATERIALS AND METHODS: Isolates of Actinobacteria were separated from soils around oil mills using four isolation media, among them three were enriched by olive pomace...
March 2017: Iranian Journal of Biotechnology
Arezoo Tavakoli, Ainon Hamzah
BACKGROUND: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. OBJECTIVES: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized. MATERIALS AND METHODS: The xylC was amplified and cloned in E. coli. The recombinant plasmid pGEMT-xylC was digested by NdeI and HindIII to construct plasmid pET28b-xylC and transformed in E...
March 2017: Iranian Journal of Biotechnology
Bahareh Attaran, Tahereh Falsafi
BACKGROUND: A few reports confirm the ability of Helicobacter pylori to form biofilm. However, conclusive data do not exist concerning the factors that favor this ability. OBJECTIVES: Evaluation of the factors associated with the biofilm formation ability of H. pylori including bacterial, physical and chemical, and environmental factors was the research's aim. MATERIALS AND METHODS: H. pylori isolates from gastric biopsy specimens of patients infected chronically were screened for biofilm formation ability...
March 2017: Iranian Journal of Biotechnology
Kourosh Maboudi, Seyedeh Marzieh Hosseini, Mina Sepahi, Hashem Yaghoubi, Shahin Hadadian
BACKGROUND: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require specifi c antibodies. High cost, inappropriate shipping (cold chain failures), reduced sensitivity and thus poor detection performance are common pitfalls of using commercial kits for performing immunological tests...
March 2017: Iranian Journal of Biotechnology
Niloofar Zandvakili, Mohammadreza Zamani, Mostafa Motallebi, Zahra Moghaddassi Jahromi
BACKGROUND: Plants have various defense mechanisms such as production of antimicrobial peptides, particularly pathogenesis related proteins (PR proteins). PR10 family is an essential member of this group, with antifungal, antibacterial and antiviral activities. OBJECTIVE: The goal of this study is to assess the antifungal activity of maize PR10 against some of fungal phytopathogens. MATERIALS AND METHODS: Zea mays PR10 gene (TN-05-147) was cloned from genomic DNA and cDNA and overexpressed in Escherichia coli...
March 2017: Iranian Journal of Biotechnology
Habib Rezapoor, Mahnaz Aghdasi, Hamid Reza Sadeghipoor
BACKGROUND: TRR14 protein is a small protein, a member of a multigene family in Arabidopsis which was found as the fi rst protein during screening seedlings for their resistant to the trehalose sugar. OBJECTIVES: A number of TRR14-overexpressing plants were subjected to the characterization in the present research, among which, the associated morphological features and changes accompany growth pattern and photosynthesis related parameters. MATERIALS AND METHODS: TRR14 gene was isolated from Arabidopsis Thaliana and cloned into the pBin-35S vector...
March 2017: Iranian Journal of Biotechnology
Behzad Hajieghrari, Naser Farrokhi, Bahram Goliaei, Kaveh Kavousi
BACKGROUND: Micro RNAs (miRNAs) are a pivotal part of non-protein-coding endogenous small RNA molecules that regulate the genes involved in plant growth and development, and respond to biotic and abiotic environmental stresses posttranscriptionally. OBJECTIVE: In the present study, we report the results of a systemic search for identification of new miRNAs in B. rapa using homology-based ESTs (Expressed Sequence Tags) analysis and considering a series of fi ltration criteria...
March 2017: Iranian Journal of Biotechnology
Zahra Zamanzadeh, Mitra Ataei, Seyed Massood Nabavi, Ghasem Ahangari, Mehdi Sadeghi, Mohammad Hosein Sanati
BACKGROUND: Multiple sclerosis (MS) is the most common autoimmune disease of the central nervous system (CNS). The main cause of the MS is yet to be revealed, but the most probable theory is based on the molecular mimicry that concludes some infections in the activation of T cells against brain auto-antigens that initiate the disease cascade. OBJECTIVES: The Purpose of this research is the prediction of the auto-antigen potency of the myelin proteolipid protein (PLP) in multiple sclerosis...
March 2017: Iranian Journal of Biotechnology
Lin Hua, Hong Xia, Wei-Ying Zheng, Li An
BACKGROUND: Transforming growth factor (TGF)-β is over-expressed in a wide variety of cancers such as lung adenocarcinoma. TGF-β plays a major role in cancer progression through regulating cancer cell proliferation and remodeling of the tumor micro-environment. However, it is still a great challenge to explain the phenotypic effects caused by TGF-β stimulation and the effect of TGF-β stimulation on tumor micro-environment. OBJECTIVES: To address this issue, in the present study we used two time-course microarray data in human lung adenocarcinoma cells and applied bioinformatics methods to explore the gene regulation network responding to TGF-β stimulation in lung adenocarcinoma cells...
March 2017: Iranian Journal of Biotechnology
Seyyed Hossein Vafaei, Maryam Mahmoodi
Background: Cucurbit aphid-borne yellows virus (CABYV) is among the most important yellowing viruses on cucurbits in Iran. The presence of CABYV has been previously reported from the major cucurbit growing areas in Iran, however, there are few studies concerning the detection of the different strains of this virus in the grower's fields, and especially, there is no report of the weed host plants near crop fields. Objectives: This study was done in order to detect the new strains of the CABYV polerovirus in cucurbits and the weed plants in the Lorestan province, Iran, as an introductory investigation for initiating a program of the breeding for resistance...
2017: Iranian Journal of Biotechnology
Javad Karimi, Sasan Mohsenzadeh, Ali Niazi, Ali Moghadam
Background: The increasing use of nanoparticles (NPs) may have negative impacts on both organisms and the environment. Objectives: The differential expression of mitochondrial manganese superoxide dismutase ( MnSOD ) gene in wheat in response to silver nitrate nanoparticles (AgNPs) and AgNO3 was investigated. Materials and Methods: A quantitative Real-Time RT-PCR experiment was carried out with MnSOD gene using RNAs isolated from wheat shoots treated for 0, 2, 6, 12, and 24 h with 100 mg.L-1 of either AgNO 3 or AgNPs...
2017: Iranian Journal of Biotechnology
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