Ingrid Skornova, Tomas Simurda, Lucia Stanciakova, Viliam Lauko, Pavol Holly, Matej Samos, Tomas Bolek, Martin Schnierer, Miroslava Drotarova, Kristina Maria Belakova, Juraj Sokol, Jan Stasko, Marian Mokan, Jaroslav Gumulec, Leona Chrastinova
Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT IgG, ZYMUTEST HIA Monostrip IgG) to detect the presence of antibodies against heparin-PF4 complexes (platelet factor 4). When the immunoassay was positive, the sample was subsequently analyzed for HIT with a functional flow cytometry assay, the HITAlert kit, the purpose of which was to demonstrate the ability of the antibodies present to activate platelets...
September 21, 2023: Diagnostics