Rainer Kaiser, Christoph Gold, Markus Joppich, Quentin Loew, Anastassia Akhalkatsi, Tonina T Mueller, Felix Offensperger, Augustin Droste Zu Senden, Oliver Popp, Lea di Fina, Viktoria Knottenberg, Alejandro Martinez-Navarro, Luke Eivers, Afra Anjum, Raphael Escaig, Nils Bruns, Eva Briem, Robin Dewender, Abhinaya Muraly, Sezer Akgöl, Bartolo Ferraro, Jonathan K L Hoeflinger, Vivien Polewka, Najib Ben Khaled, Julian Allgeier, Steffen Tiedt, Martin Dichgans, Bernd Engelmann, Wolfgang Enard, Philipp Mertins, Norbert Hubner, Ludwig Weckbach, Ralf Zimmer, Steffen Massberg, Konstantin Stark, Leo Nicolai, Kami Pekayvaz
Neutrophils rapidly respond to inflammation and infection, but to which degree their functional trajectories after mobilization from the bone marrow are shaped within the circulation remains vague. Experimental limitations have so far hampered neutrophil research in human disease. Here, using innovative fixation and single-cell-based toolsets, we profile human and murine neutrophil transcriptomes and proteomes during steady state and bacterial infection. We find that peripheral priming of circulating neutrophils leads to dynamic shifts dominated by conserved up-regulation of antimicrobial genes across neutrophil substates, facilitating pathogen containment...
March 22, 2024: Science Advances