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Balsaeng'gwa Saengsig

In-Seok Park, Hyun Woo Gil
To clarify the cytogenetic features of the chicken grunt, Parapristipoma trilineatum , small yellow croaker, Larimichthys polyactis , and brown croaker, Miichthys miiuy , in Jeju, Korea, karyotype analysis was performed. The karyotype of chicken grunt and brown croaker consists of 24 pairs of telocentrics [2n=48, fundamental number (FN)=48], and small yellow croaker consists of 3 pairs of submetacentrics and 21 pairs of telocentrics (2n=48, FN=54). No evidence of polymorphism, including aneuploidy or sex-related heteromorphic chromosomes, was found in any specimens examined in this study...
March 2019: Balsaeng'gwa Saengsig
Haewon Jeon, Jiyun O, Sil Jin, Jinsung Lim, Chong Pyo Choe
Thehead gap gene buttonhead ( btd ) is required for the patterning of head segments in the early Drosophila embryo. Mutant phenotypes of btd display a gap-like phenotype in which antennal, intercalary, mandibular and the anterior portion of the maxillary segmentsare eliminated. In agreement with the phenotypes, btd is expressed in a stripe covering the head segments at the blastoderm stage. During the early phase of the germband extension, btd is expressed in stripes with single segmental periodicity, which is required for the formation of the peripheral nervous system...
March 2019: Balsaeng'gwa Saengsig
Hee Jeong Kong, Julan Kim, Ju-Won Kim, Hyun-Chul Kim, Jae Koo Noh, Young-Ok Kim, Woo-Jin Kim, Sang-Yeob Yeo, Jung Youn Park
To develop a promoter capable of driving transgene expression in non-model fish, we identified and characterized the muscle-specific alpha-actin gene in olive flounder, Paralichthys olivaceus ( PoACTC1 ). The regulatory region of PoACTC1 includes putative regulatory elements such as a TATA box, two MyoD binding sites, three CArG boxes, and a CCAAT box. Microinjection experiments demonstrated that the regulatory region of PoACTC1 , covering from -2,126 bp to +751 bp, just prior to the start codon, drove the expression of red fluorescent protein in developing zebrafish embryos and hatching olive flounder...
March 2019: Balsaeng'gwa Saengsig
Yeo-Reum Park, Hye-Bin Park, Mi-Jeong Kim, Bae-Dong Jung, Seunghyung Lee, Choon-Keun Park, Hee-Tae Cheong
We examined the effects of endoplasmic reticulum (ER) stress inhibitor treatment during the micromanipulation of porcine somatic cell nuclear transfer (SCNT) on the in vitro development of SCNT embryos. ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxycholic acid (TUDCA; 100 μM) were added to the micromanipulation medium and holding medium. The expression of X-box binding protein 1 ( Xbp1 ), ER-stress-associated genes, and apoptotic genes in SCNT embryos was confirmed at the one-cell and blastocyst stages...
March 2019: Balsaeng'gwa Saengsig
Seong Hee Mun, Jin Ho You, Hyeon Ji Oh, Chi Hoon Lee, Hea Ja Baek, Young-Don Lee, Joon Yeong Kwon
Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In this study, we investigated differences of GH, IGF1 and GHR gene expressions in juvenile red spotted grouper ( Epinephelus akaara ) with different growth performance. Red spotted groupers were reared under the same environmental condition (water temperature 24±1℃, natural light) for 96 days after hatching...
March 2019: Balsaeng'gwa Saengsig
In-Seok Park
The effects of the anaesthetic agents, clove oil and mixture of clove oil with lidocaine-HCl were evaluated on river puffer, Takifugu obscurus and tiger puffer, T. rubripes . Anaesthesia times of clove oil were affected by water temperature (20℃, 24℃, and 28℃) and salinity (10, 20, and 30 ppt). Anaesthesia times of mixed samples were significantly similar with regard to exposure and recovery times, and all samples satisfied anaesthesia criteria (exposure time within 3 min and recovery time within 5 min) under the various temperatures and salinities, and the lowest to highest concentration of anaesthetics ( p <0...
March 2019: Balsaeng'gwa Saengsig
Won-Hee Lee, Wook-Hwan Kim, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
The study was conducted to investigate the effects of alpha-linolenic acid (ALA) combined with bovine serum albumin (BSA) or methyl-beta-cyclodextrin (MBCD) on plasma and acrosomal membrane damages, mitochondrial activity, morphological abnormality, motility, and oxidative stress in frozen-thawed boar sperm. In previous our study, 3 ng/mL ALA had been shown protective effect during freezing process of boar sperm. Therefore, we used 3 ng/mL ALA in present study and ALA was combined with same molar ratio of BSA or MBCD (ALA+BSA and ALA+MBCD, respectively)...
March 2019: Balsaeng'gwa Saengsig
Sook Young Yoon
The ability of oocytes to undergo normal fertilization and embryo development is acquired during oocyte maturation which is transition from the germinal vesicle stage (GV), germinal vesicle breakdown (GVBD) to metaphase of meiosis II (MII). Part of this process includes redistribution of inositol 1,4, 5-triphosphate receptor (IP3R), a predominant Ca2+ channel on the endoplasmic reticulum membrane. Type 1 IP3R (IP3R1) is expressed in mouse oocytes dominantly. At GV stage, IP3R1 are arranged as a network throughout the cytoplasm with minute accumulation around the nucleus...
March 2019: Balsaeng'gwa Saengsig
Hye-Bin Park, Mi-Jeong Kim, Bae-Dong Jung, Seunghyung Lee, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
[This corrects the article DOI: 10.12717/DR.2018.22.3.235.].
December 2018: Balsaeng'gwa Saengsig
Young Sik Kim, Jong-Man Yoon
Genomic DNA was isolated from the Gracilaria vermiculophylla (GRV) and G. chorda (GRC) from Jangheung located in the southern sea of the Korean Peninsula, respectively and we performed clustering analyses, DNA polymorphisms and the genetic differences. The seven selected primers OPC-01, OPA-04, OPA-05, OPD-07, OPD-08, OPB-10, and OPD-16 generated average bandsharing (BS) value, the genetic distance and dendrogram. The size of DNA bands varies from 90 bp to 2,400 bp. The average BS value was 0.859±0.004 within GRV and 0...
December 2018: Balsaeng'gwa Saengsig
Juhye Kim, Sunyeong Cha, Min Young Lee, Yeon Jeong Hwang, Eunhyeok Yang, Chongsuk Ryou, Hyo-Il Jung, Yong-Pil Cheon
Through the development of organic synthetic skill, chemicals that mimic signaling mediators such as steroid hormones have been exposed to the environment. Recently, it has become apparent that this circumstance should be further studied in the field of physiology. Estrogenic action of chronic low-dose nonylphenol (NP) and di-(2-ethylhexyl) phthalate (DEHP) in mouse uterus was assessed in this study. Ten to twelve-week-old female mice (CD-1) were fed drinking water containing NP (50 or 500 μg/L) or DEHP (133 or 1,330 μg/L) for 10 weeks...
December 2018: Balsaeng'gwa Saengsig
Jong Youn Park, Jae Kwon Cho, Young Jae Choi, Kyeong Ho Han, Chang Gi Hong
We determined the morphologic characteristics (body weight and degree of abdomen inflation) of the red spotted grouper, Epinephelus akaara , mother fish producing healthy eggs. Experimental fish were chosen from fish reared in a sea cage. The fish were divided into four size groups by body weight: 400~600, 600~800, 800~1,000, and 1,000~1,200 g and four stages (Ⅰ~Ⅳ) of the degree of abdomen inflation. After hormone treatment, we observed the amount of ovulation-induced eggs, and rates of buoyancy, fertilization, embryonic survival, and hatching...
December 2018: Balsaeng'gwa Saengsig
Gi-Seok Lee, Sung-Hun Lee, Jin Lee, Jea-Min Park, Kyeong-Ho Han
The newborn, Hippocampus kuda larvae were 6.97-8.81, standard length (SL) mm (mean 7.89 mm) and mouth and anuse were open. Dorsal fin rays 15-18 and pectoral fin-rays were counted 8 and had 10-11+21 myotomes, body's bony plate ring being developed strongly in the central axis of myotomes part. 4 days after bearing, the SL was 7.02-9.47 mm (mean 8.24 mm) and nostrils began to open. 12 days after bearing, larvae attained to 8.91-11.2 SL mm (mean 10.0 mm). From this time, their unique predation habit appeared...
December 2018: Balsaeng'gwa Saengsig
Ki-Ho Lee, Nan Hee Kim
The adipogenesis is a maturation process of pre-adipocyte cell into mature lipid-filled adipocyte cell. The adipogenesis begins at the late prenatal stage and continues until the early postnatal age. Because the adipogenesis and formation of adipose tissue persist during postnatal period and are precisely regulated by the action of numerous gene products, the present research was attempted to determine the expressional patterns of adipose tissue-associated genes in the rat epididymal fat pad at different postnatal ages, from 7 days to 2 years of ages, using a quantitative real-time PCR analysis...
December 2018: Balsaeng'gwa Saengsig
Kyung-Hee Kim, Woo-Jin Kim, Choul-Ji Park, Jong-Won Park, Gyeong Eon Noh, Seunghyung Lee, Young Mee Lee, Hyun Chul Kim
Chemokines is a small protein that plays a major role in inflammatory reactions and viral infections as a chemotactic factor of cytokines involved in innate immunity. Most of the chemokines belong to the chemokine groups CC and CXC. To investigate the immune system of the olive flounder ( Paralichthys olivaceus ), an expression pattern specifically induced in the early developmental stages of analysis is examined using qRT-PCR. We also examined tissue-specific expression of both CC and CXC chemokine in healthy olive flounder samples...
December 2018: Balsaeng'gwa Saengsig
Sojung Sun, Hyunwon Yang
NUCB2/nesfatin-1 is first known to be expressed in the hypothalamus while controlling appetite and energy metabolism. However, recent studies have shown that NUCB2/nesfatin-1 was expressed in the various organs as well as the hypothalamus. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the ovary, testis, pituitary gland, lung, kidney, and stomach of fetal and adult mice. However, the role of NUCB2/nesfatin-1 in mouse fetus remains unknown. Thus, the aim of this study was to investigate whether NUCB2/nestatin-1 is expressed in mouse fetus at the developmental stage in which organogenesis begins...
December 2018: Balsaeng'gwa Saengsig
In-Seok Park, Hyun Woo Gil
The experiment was conducted for 210 days to determine the effect of feeding, and starvation, and exposure to sodium nitrite (NaNO2 ) on the survival, physiological changes, hematological parameter, and stress response of Far Eastern catfish, Silurus asotus . The survival of the starved group was lower than that of the fed group during the experiment. Starvation resulted in retardation of growth, which provides an example of fish that failed to continue to grow and remain in a good condition. Blood analyses (cortisol and glucose) showed significant differences of stress response between the fed and starved groups exposed to NaNO2 at the conclusion of the experiment ( p <0...
December 2018: Balsaeng'gwa Saengsig
Yong Hwangbo, Mi-Rim Lee, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
The present study was conducted to investigate the regulatory mechanism of plasminogen activators (PAs) activation by 17β-estradiol (E2 ) and progesterone (P4 ) in porcine uterine epithelial cells (pUECs). pUECs were collected from porcine uterine horn and cultured at 80% confluence. Then, 0.1% (v/v) DMSO, 20 ng/mL E2 , and P4 with or without fetal bovine serum (FBS) treated to cultured cells for 24 hours. The supernatants were used for measurement of PAs activity and expression of urokinase-type PA ( uPA ), tissue-type PA ( tPA ), uPA specific receptor ( uPAR ), and type-1 PA inhibitor ( PAI-1 ) mRNA were analyzed by real-time PCR...
December 2018: Balsaeng'gwa Saengsig
Ji-Eun Lee, Yong Hwangbo, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
The objective of this study was to evaluate the effects of alpha-linolenic acid (ALA) during in vitro maturation (IVM) on cumulus expansion, nuclear maturation, fertilization capacity and subsequent development in porcine oocytes. The oocytes were incubated with 0, 25, 50, and 100 μM ALA. Cumulus expansion was measured at 22 h, and gene expresison and nuclear maturation were analyzed at 44 h after maturation. Then, mature oocytes with ALA were inseminated, and fertilization parameters and embryo development were evaluated...
December 2018: Balsaeng'gwa Saengsig
Hyeon Ji Oh, Jung-Hyun Kim, Seong Hee Mun, Jin Hui Kim, Dae-Jung Kim, Joon Yeong Kwon
Large quantity of eggs fail to be fertilized and many of fertilized eggs are unable to hatch in the eel, Anguilla japonica . Larvae of eel absorb egg yolk up to 8 days after hatching but the majority of hatched larvae die before they reach the stage of first feeding in this species. Genes of key enzymes for yolk processing (cathepsin B, D, L and lipoprotein lipase - abbreviated as ctsb, ctsd, ctsl and lpl , respectively) could be associated with egg quality. In this study, we investigated differences in the expression of these genes between floating eggs and sinking eggs, and also the relationship between the gene expressions of the enzymes and fertilization rates in the fertilized eggs obtained from artificially matured female eels...
September 2018: Balsaeng'gwa Saengsig
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