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Veterinary Research Forum

Enrico Pierluigi Spugnini, Andrea Lanza, Silvia Sebasti, Alfonso Baldi
A five-year-old female African hedgehog ( Atelerix albiventris ) was referred for a one month growing oral mass. The hedgehog was quiet, alert and responsive, with a 1.00 × 1.50 cm ulcerated lesion on the mandible. The patient was staged with total body radiographs, hematological and biochemical analyses and the mass was biopsied under anesthesia. The excised tissue was reported to be a squamous cell carcinoma. At this time, due to the extension of the mass, a surgical excision was not a therapeutic option and the tumor was palliated with electrochemotherapy (ECT)...
2018: Veterinary Research Forum
Saeed Seifi, Hojjatollah Shokri, Mahvash Karimi Madab
Fungal infections cause significant economic losses in the poultry industry either due to their direct infectious nature or due to mycotoxins production. Hatchery contamination with fungi can threaten chicken health. In this regard, geographical and seasonal distributions of airborne fungal contamination of 25 hatcheries in Mazandaran province, northern Iran, were investigated using an open plate method. The results of this study showed that hatcheries have various fungal contaminations, among which the most common were respectively Cladosporium (31...
2018: Veterinary Research Forum
Eshrat Beigom Kia, Mitra Sharbatkhori, Zahra Heidari, Farideh Tohidi, Bahareh Kamran Rashani, Farzaneh Zahabiun
A polycephalic larva of Taeniidae family isolated from abdominal cavity of a great gerbil ( Rhombomys opimus ) from Golestan province, northern Iran, was subjected to molecular analysis. Genomic DNA from the larva was obtained using a DNA extraction tissue kit. Polymerase chain reaction was performed for amplification of the partial 12S rRNA, cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. BLAST analysis of DNA sequencing indicated 99.00% homology in 12S rRNA and cox1 genes and 98...
2018: Veterinary Research Forum
Javad Aliakbarlu, Sindokht Ghiasi, Behnaz Bazargani-Gilani
Barberry fruit is consumed in different forms including dried fruit, juice, jam and marmalade in Iran. This fruit is also used as a food additive (flavoring and colorant) in soup and rice dishes. In present study, antioxidant activities of acetone, ethanol and water (infusion and decoction) extracts of barberry ( Berberis vulgaris ) fruit were investigated using 2,2-azinobis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power methods. Total phenolic contents of the extracts were also estimated using Folin-Ciocalteu assay...
2018: Veterinary Research Forum
Javad Alikhani, Godratollah Mohammadi, Gholamreza Shariati
The domestic water buffalo (Bubalus bubalis) is an economically important livestock species in many Asian and Mediterranean countries. Two types of water buffalos are river and swamp buffaloes. Genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = 50). Khuzestan province is one of the important centers of buffalo farming in Iran. Since there was no cytogenetic information about Khuzestani buffalo breed, the present study was performed to provide karyotype and cytogenetic study in Khuzestan buffaloes...
2018: Veterinary Research Forum
Abdolrasoul Namjou, Esfandiar Heidarian, Mahmoud Rafieian-Kopaei
In this study, the effects of Urtica dioica hydro-alcoholic extract were investigated on the blood glucose and lipid profiles of female ovariectomized and non-ovariectomized rats. In total, 32 adult female rats were divided into four groups (eight each) including control and ovariectomy groups as well as non-ovariectomy and ovariectomy groups treated with 200 mg kg-1 of Urtica dioica extract orally in the last five weeks of the study starting from the week 56th . The duration of the study was 60 weeks. Glucose, serum lipid profiles and pancreatic pathological alterations were determined in these groups at the end of experiment...
2018: Veterinary Research Forum
Reza Narenji Sani, Bahram Hajigolikhani, Mahmood Ahmadi-Hamedani, Khatereh Kafshdouzan
The aim of the present study was to investigate the diagnostic evaluation of milk lactate dehydrogenase (mLDH) and alkaline phosphatase (mALP) activities by receiver operating characteristic (ROC) analysis curve in early lactation of ewes with subclinical mastitis (SCM) and determine the correlation between number of somatic cell count (SCC) and mLDH and mALP activities. A total of 196 udder half milk samples were collected within the first 6 weeks of lambing. The SCM was determined by positive milk bacterial culture and positive California mastitis test (CMT); SCC was determined by fossomatic method and enzyme activities were determined spectrophotometrically...
2018: Veterinary Research Forum
Salah Nadimi, Mohammad Molazem, Seyedhosein Jarolmasjed, Mohammad Reza Esmaili Nejad
The objective of this study was to evaluate the pituitary gland dimensions due to age and weight using computed tomography (CT) in dogs and cats. The CT images of pituitary gland were assessed in 11 client-owned dogs (six males and five females; age range, 1 to 9 years) and 15 client-owned cats (eight males and seven females; age range, 1 to 14 years) with no evidence of pituitary diseases. The length, height, width and volume of the pituitary gland were measured in sagittal and transverse planes. Mean pituitary length, width, height and volume (± standard deviation: SD) were respectively 4...
2018: Veterinary Research Forum
Esmaeal Tamaddonfard, Sina Tamaddonfard, Siamak Cheraghiyan
Vitamin B12 modulates pain at the local and peripheral levels. This study has investigated the effects of intracerebroventricular (ICV) injection of vitamin B12 on themuscle pain. We used diclofenac (cyclooxygenase inhibitor) and naloxone (opioid receptors antagonist) to clarify the possible mechanisms. For ICV injections, a guide cannula was implanted in the left lateral ventricle of the brain. Muscle pain was induced by intramuscular injection of formalin (2.50%; 50 µl) in the right gastrocnemius muscle and the number of paw flinching was recorded at 5-min blocks for 60 min...
2018: Veterinary Research Forum
Sara Khalili, Mehdi Mohebali, Elaheh Ebrahimzadeh, Ebrahimzadeh Shayan, Samira Mohammadi-Yeganeh, Mehrdad Moosazadeh Moghaddam, Samira Elikaee, Behnaz Akhoundi, Mohammad Kazem Sharifi-Yazdi
Zoonotic cutaneous leishmaniasis caused by Leishmania major is a most common type of vector-borne disease in Iran. The pentavalent antimonial drugs have been used in the treatment of cutaneous leishmaniasis for a long time, but drug resistance and some of serious side effects have been reported. Thus, discovery and development of new therapeutic candidates are needed. The CM11 peptide is one of these peptides that its anti-bacterial activity has been proven. This peptide is a short cecropin-melittin hybrid peptide obtained through a sequence combination approach...
2018: Veterinary Research Forum
Mohammad Abbaszadeh Hasiri, Effat Baghaei Moghaddam, Mohammad Reza Khalili, Amin Hossein Amini, Masoomeh Eghtedari, Mohammad Azizzadeh, Hooman Razmi
Uveitis is a major cause of vision loss. Methotrexate (MTX) has been widely used in uveitis due to its relatively safe profile. The purpose of this study was to evaluate the effects of two different dosages of MTX via intra-vitreal administration for treatment of endotoxin induced uveitis (EIU) in an experimental model. Thirty-five healthy rabbits were randomly divided into four groups and all animals were tolerated intra-vitreal injections. The first group received normal saline (NS), the second group received normal saline plus Salmonella typhimurium lipopolysaccharide endotoxin (LPS), (NS+LPS), the third group received 400 μg MTX plus LPS (LPS+MTX 400) and the fourth group received 800 μg MTX plus LPS (LPS+MTX 800)...
2018: Veterinary Research Forum
Homayoun Naderain, Neda Khanlarkhani, Iraj Ragerdi Kashani, Amirabbas Atlasi, Mohammad Ali Atlasi
Steroids promote the myelination and regeneration in the peripheral nervous system. Whereas, little is known about the inducing effects by which the hormones exert their effects on Schwann cells differentiation. This could be revealed by the expression of Schwann cell markers in adipose-derived stem cells (ADSCs). The purpose of this study was to present the effects of progesterone and 17 β-estradiol on the Schwann cell markers in rat ADSCs. The mesenchymal stem cell markers (CD73, and CD90) were assayed by flow cytometry...
2018: Veterinary Research Forum
Mobin Koohestani, Mehran Moradi, Hossein Tajik, Armen Badali
This study was carried out to investigate the stability, antibacterial properties and biofilm removal potential of cell-free supernatant (CFS) of Lactobacillus acidophilus LA5 and Lactobacillus casei 431 against Staphylococcus aureus ATCC 25923. Antibacterial activity of both Lactobacillus strains was measured according to the agar spot method. The CFS was prepared by centrifugation of bacterial suspension at 4000 g for 10 min and the antimicrobial activity was measured using agar-well diffusion. The stability of CFSs during storage at 4...
2018: Veterinary Research Forum
Jaime Sardá Aramburú Junior, Tiago Luis Eilers Treichel, Saulo Tadeu Lemos Pinto Filho, Sergio Alexandre Gehrke, Alencar Kolinski Machado, Francine Carla Cadoná, Ivana Beatrice Mânica da Cruz, Ney Luis Pippi
The aim of this study was to evaluate the potential use of a DNA comet assay, DNA fragmentation fluorimetric assay and reactive oxygen species levels as potential biomarkers of genome conditions of dental pulp stem cells (DPSCs) isolated from dog canine teeth. Mesenchymal stem cells were isolated from the dental pulp collected from dog teeth. The results obtained suggest the ideal moment for clinical application of cellular therapy for this type of cell. The cell culture was maintained with Dulbecco's modified Eagle's medium supplemented with 10...
2018: Veterinary Research Forum
Ali Parchami, Mahdi Salimi, Mehrdad Khosravi
The aim of this study was to investigate the dorsal surface of the long-eared hedgehog tongue using scanning electron microscopy. The tongue of the long-eared hedgehog is rectangular-shaped with an elongated body and a widened root and a marked deep median groove can be seen on its dorsal surface. A characteristic feature of the lingual root is presence of two huge laterally situated folds. These anatomical structures have never been described with regard to other small mammals studied so far. According to their anatomical appearances, the lingual papillae can be distinguished as filiform, conical, fungiform, circumvallate and foliate papillae...
2018: Veterinary Research Forum
Akbar Asadi, Taghi Zahraei Salehi, Mahmoud Jamshidian, Reza Ghanbarpour
Avian pathogenic Escherichia coli (APEC) are responsible for wide ranges of extra-intestinal diseases in poultry including colibacillosis, cellulitis, coligranuloma and yolk sac infection. Numbers of virulence are considered important in the pathogenicity of these diseases. The aims of the present study were phylogenetic typing and virulence genes detection in Escherichia coli isolates from colibacillosis and cellulitis of broiler chickens in poultry slaughterhouses of Shahrbabak region, Kerman, Iran. A total number of eighty three E...
2018: Veterinary Research Forum
Abdollah Derakhshandeh, Fatemeh Namazi, Elmira Khatamsaz, Vida Eraghi, Zahra Hemati
In the present study, Mycobacterium avium subsp . paratuberculosis (MAP) was investigated in goats slaughtered in Shiraz abattoir using histopathological examinations and polymerase chain reaction (PCR). Ilium and mesenteric lymph node samples from 66 suspected goat carcasses to Johne's disease were collected. Among 66 examined slaughtered goats, nine (13.63%) goats were positive for MAP in both histopathological and PCR examinations. Eight goats were positive in PCR method while no lesion related to Johne's disease was observed in their histopathological sections...
2018: Veterinary Research Forum
Mohammad Farajli Abbasi, Mohammad Mahdi Molaei, Reza Kheirandish, Ali Mostafavi
This study was conducted to evaluate the effect of subcutaneous administration of synthetic eugenol (EG) for disbudding of goat kids, as a new chemical method. Thirty apparently healthy Raieni (Cashmere) goat kids (five-day-old) were divided randomly into six groups (n = 5). In the pathology (P) groups (P3 , P8 and P60 according to the sample collection day after injection) an amount of 0.10 mL of EG was subcutaneously administrated in both horn buds. In the disbudding 1 and 2 (DB1 , DB2 ) groups, 0.10 mL of EG, and in the control (C) group 0...
2018: Veterinary Research Forum
Ghasem Farjanikish, Omid Dezfoulian, Hossein Mohammadi
A four-year-old male Persian cat was referred with three weeks history of progressive lameness due to a rigid osseous mass with 3.50×2.50×2.00 cm in dimensions in his left arm. In the histopathological evaluation of bone biopsy, two distinct populations of cells including multinucleated giant cells and oval cells which embedded in a fibro-osseous stroma and surrounded by lamellar bone trabeculae were observed. At necropsy, multiple metastatic nodules with different sizes unveiled in the liver and spleen. Microscopically, those tumor cells which already described in the bone lesion were also infiltrated to the liver and spleen...
2018: Veterinary Research Forum
Mohammad Khezri, Mojtaba Moharrami, Hossain Modirrousta, Maryam Torkaman, Saleh Salehi, Babak Rokhzad, Homan Khanbabai
Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of Nosema species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of Nosema species infecting European honeybee, Apis mellifera . A total of 100 samples were collected from apiaries (870 hives) in 10 counties of Kurdistan province, located in the west of Iran...
2018: Veterinary Research Forum
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