journal
https://read.qxmd.com/read/37583477/a-high-throughput-screening-process-and-quick-isolation-of-novel-lignin-degrading-microbes-from-large-number-of-natural-biomasses
#21
JOURNAL ARTICLE
Nadia Sufdar Ali, Fang Huang, Wensheng Qin, Trent Chunzhong Yang
High throughput screening approaches can significantly speed up the identification of novel enzymes from natural microbial consortiums. A two-step high throughput screening process was proposed and explored to screen lignin-degrading microorganisms. By employing this modified culture enrichment method and screening based on enzyme activity, a total of 82 bacterial and 46 fungal strains were isolated from fifty decayed wood samples (100 liquid cultures) collected from the banks of the Ottawa River in Canada...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37559690/inhibition-of-bacterial-biofilms-by-the-snake-venom-proteome
#22
JOURNAL ARTICLE
Neyaz A Khan, Fernanda G Amorim, John P Dunbar, Dayle Leonard, Damien Redureau, Loïc Quinton, Michel M Dugon, Aoife Boyd
Snake venoms possess a range of pharmacological and toxicological activities. Here we evaluated the antibacterial and anti-biofilm activity against methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA) of venoms from the Samar spitting cobra Naja samarensis and the Puff adder Bitis arietans. Both venoms prevented biofilm production by pathogenic S. aureus in a growth-independent manner, with the B. arietans venom being most potent. Fractionation showed the active molecule to be heat-labile and >10 kDa in size...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37528864/detergent-stable-amylase-production-by-paenibacillus-lactis-strain-opsa3-isolated-from-soil-optimization-by-response-surface-methodology
#23
JOURNAL ARTICLE
Emmanuel Tobechukwu Ugwuoji, Tochukwu Nwamaka T Nwagu, Lewis Iheanacho Ezeogu
This study aimed to isolate thermostable, alkaliphilic, and detergent-tolerant amylase-producing bacteria. Pure isolates from environmental samples were screened on a starch-based medium (pH 11), and selected isolates were identified using cultural and molecular techniques. Product optimization studies were conducted, and secreted amylase was partially purified using 40% (w/v) saturation ammonium sulfate at 4 °C. The wash performance of concentrated amylase was analyzed. A novel isolate, Paenibacillus lactis OPSA3, was selected for further studies...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37448785/prospects-and-challenges-of-utilizing-sugarcane-bagasse-as-a-bio-coagulant-precursor-for-water-treatment
#24
REVIEW
Kingsley O Iwuozor, Adewale George Adeniyi, Ebuka Chizitere Emenike, Toluwalase Ojeyemi, Abel U Egbemhenghe, Chika J Okorie, Bridget Dunoi Ayoku, Oluwaseyi Damilare Saliu
Sugarcane bagasse is an abundant and renewable agricultural waste material generated by the sugar industry worldwide. The use of sugarcane bagasse as a bio-coagulant precursor in water treatment is an eco-friendly and cost-effective approach that has shown great potential. This article reviewed the prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment. The article reviewed past studies and explored the properties and chemical composition of sugarcane bagasse and the bioactive compounds that can be extracted from it, as well as their potential coagulation performance in water treatment...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37448784/from-purposeless-residues-to-biocomposites-a-hyphae-made-connection
#25
JOURNAL ARTICLE
Isabel Enriquez-Medina, Andres Ceballos Bermudez, Erika Y Ortiz-Montoya, Carlos Alvarez-Vasco
Biocomposites create attractive alternatives to match packing needs with available agricultural residues. Growing native fungal strains developed a mycelium biocomposite over a mixture of Peach Palm Fruit Peel Flour and Sugar Cane Bagasse Wet Dust. A methodology was proposed to analyze their main characteristics: 1) morphological, 2) chemical, and 3) biodegradability. 1) SEM analysis evidenced the structural change of the dried vs pressed material and mycelium morphology for both species. 2) The ratio lignin:carbohydrate showed that  P...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37415835/fortification-of-chlorella-vulgaris-with-citrus-peel-amino-acid-for-improvement-biomass-and-protein-quality
#26
JOURNAL ARTICLE
Zhila Heydari Koochi, Kourosh Ghodrat Jahromi, Gholamreza Kavoosi, Asghar Ramezanian
The amino acid extract (protein hydrolysate) from various citrus peels was employed as an organic nutrition source for the culture of Chlorella to investigate their effects on the biomass and protein quality of the microalgae. The major amino acids in citrus peels included proline, asparagine, aspartate, alanine, serine, and arginine. The most plentiful amino acids in the Chlorella were alanine, glutamic acid, aspartic acid, glycine, serine, threonine, leucine, proline, lysine, and arginine. Adding the citrus peel amino acid extracts to the Chlorella medium enhanced overall microalgal biomass (more than two folds p  < 0...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37388572/isolation-identification-and-screening-of-biosurfactant-producing-and-hydrocarbon-degrading-bacteria-from-oil-and-gas-industrial-waste
#27
JOURNAL ARTICLE
S Al-Marri, H I Eldos, M Y Ashfaq, S Saeed, S Skariah, L Varghese, Y A Mohamoud, A A Sultan, M M Raja
Qatar is one of the biggest oil and gas producers in the world, coupled with it is challenging environmental conditions (high average temperature: >40 °C, low annual rainfall: 46.71 mm, and high annual evaporation rate: 2200 mm) harbors diverse microbial communities that are novel and robust, with the potential to biodegrade hydrocarbons. In this study, we collected hydrocarbon contaminated sludge, wastewater and soil samples from oil and gas industries in Qatar. Twenty-six bacterial strains were isolated in the laboratory from these samples using high saline conditions and crude oil as the sole carbon source...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37332617/revolutionizing-antiviral-therapy-with-nanobodies-generation-and-prospects
#28
REVIEW
Mujahed I Mustafa, Ahmed Mohammed
As the world continues to grapple with infectious diseases, scientists are constantly searching for effective ways to combat these deadly pathogens. One promising avenue of research is the use of nanobodies as neutralization agents. These small proteins, derived from camelid antibodies, have several unique advantages over traditional antibodies, including their small size. Nanobodies are much smaller than conventional antibodies, typically weighing in at around 15 kDa compared to the 150 kDa of a typical human antibody...
September 2023: Biotechnology Reports
https://read.qxmd.com/read/37849649/corrigendum-to-comparative-computational-study-to-augment-ubia-prenyltransferases-inherent-in-purple-photosynthetic-bacteria-cultured-from-mangrove-microbial-mats-in-qatar-for-coenzyme-q-10-biosynthesis-biotechnology-reports-36-2022-e00775
#29
https://read.qxmd.com/read/37234569/molecular-background-of-hac1-driven-improvement-in-the-secretion-of-recombinant-protein-in-yarrowia-lipolytica-based-on-comparative-transcriptomics
#30
JOURNAL ARTICLE
Paulina Korpys-Woźniak, Ewelina Celińska
While the unfolded protein response (UPR) and its major regulator - transcription factor Hac1 are well-conserved across Eukarya , species-specific variations are repeatedly reported. Here we investigated molecular mechanisms by which co-over-expression of HAC1 improves secretion of a recombinant protein (r-Prot) in Yarrowia lipolytica , using comparative transcriptomics. Co-over-expression of HAC1 caused an >2-fold increase in secreted r-Prot, but its intracellular levels were decreased. The unconventional splicing rate of the HAC1 mRNA was counted through transcript sequencing...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37215743/probiotics-and-their-postbiotics-for-the-control-of-opportunistic-fungal-pathogens-a-review
#31
REVIEW
S Divyashree, B Shruthi, P R Vanitha, M Y Sreenivasa
During past twenty years the opportunistic fungal infections have been emerging, causing morbidity and mortality. The fungi belonging to Aspergillus, Mucor, Rhizopus, Candida, Fusarium, Penicillium, Dermatophytes and others cause severe opportunistic fungal infections. Among these Aspergillus and Candida spp cause majority of the diseases. The continuum of fungal infections will prolong to progress in the surroundings of the growing inhabitants of immunocompromised individuals. Presently many chemical-based drugs were used as prophylactic and therapeutic agents...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37206916/extraction-and-characterization-of-bioactive-secondary-metabolites-from-lactic-acid-bacteria-and-evaluating-their-antifungal-and-antiaflatoxigenic-activity
#32
JOURNAL ARTICLE
Aya Abdel-Nasser, Amal S Hathout, Ahmed N Badr, Olfat S Barakat, Hayam M Fathy
Aflatoxins are toxic carcinogens and mutagens formed by some moulds, specifically Aspergillus spp. Therefore, this study aimed to extract and identify bioactive secondary metabolites from Lactobacillus species, to evaluate their efficacy in reducing fungal growth and aflatoxin production and to investigate their toxicity. The bioactive secondary metabolites of Lactobacillus species showed variable degrees of antifungal activity, whereas L. rhamnosus ethyl acetate extract No. 5 exhibited the highest antifungal activity and, thus, was selected for further identification studies...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37181274/antiviral-activity-of-red-algae-phycocolloids-against-herpes-simplex-virus-type-2-in-vitro
#33
JOURNAL ARTICLE
Adolfo Henríquez, Juan Pablo Vargas, Chris Landahur, Nicolás Corrales, Andrés Agurto-Muñoz, Pablo A González, Cristian Agurto-Muñoz
Herpes simplex virus type 2 (HSV-2) is a human infectious agent with significant impact on public health due to its high prevalence in the population and its ability to elicit a wide range of diseases, from mild to severe. Although several antiviral drugs, such as acyclovir, are currently available to treat HSV-2-related clinical manifestations, their effectiveness is poor. Therefore, the identification and development of new antiviral drugs against HSV-2 is necessary. Seaweeds are attractive candidates for such purposes because they are a vast source of natural products due to their highly diverse compounds, many with demonstrated biological activity...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37124472/deciphering-the-antimicrobial-antibiofilm-and-membrane-stabilizing-synergism-of-mikania-scandens-l-willd-leaves-and-stems-substantiation-through-in-vitro-and-in-silico-studies
#34
JOURNAL ARTICLE
Nadia Islam Tumpa, Md Helal Uddin Chowdhury, Ankhy Alamgir Asma
Considering the traditional application of Mikania scandens (L.) Willd. against wounds and itching. Leaves (MSL) and stems (MSS) were sequentially extracted using solvents petroleum-ether, carbon-tetrachloride, chloroform, ethyl-acetate and ethanol. Disk-diffusion assay revealed the ethyl acetate MSL and MSS extracts were the prominent against ten bacteria, five carbapenem-resistant bacteria and one fungal strains. Subsequent quantitative antimicrobial analysis specified MSL extractives more potent over MSS with lower 1500 and 3500µg/ml MIC and MBC value in both gram-negative and positive bacteria...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37064962/therapeutic-efficacy-of-plant-produced-nivolumab-in-transgenic-c57bl-6-hpd-1-mouse-implanted-with-mc38-colon-cancer
#35
JOURNAL ARTICLE
Christine Joy I Bulaon, Hongyan Sun, Ashwini Malla, Waranyoo Phoolcharoen
The therapeutic blockade of inhibitory PD-1 signaling has emerged as an effective approach for cancer immunotherapy. Nivolumab (Opdivo®), a monoclonal antibody (mAb) targeting the PD-1 immune checkpoint, is approved for treatment of several cancer indications. It functions by blocking the PD-1-mediated T-cell inhibition thus reinstating anticancer immune responses. Tremendous advances in plant biotechnology offer an alternative and economical strategy to produce therapeutic mAbs for immune-based therapies...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37056791/development-of-sars-cov-2-neutralizing-antibody-detection-assay-by-using-recombinant-plant-produced-proteins
#36
JOURNAL ARTICLE
Perawat Jirarojwattana, Balamurugan Shanmugaraj, Kaewta Rattanapisit, Waranyoo Phoolcharoen
Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme 2 (ACE2) produced from Nicotiana benthamiana for the development of an affordable neutralizing antibodies detection assay...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/37041970/probiotic-therapy-african-fermented-foods-and-food-derived-bioactive-peptides-in-the-management-of-sars-cov-2-cases-and-other-viral-infections
#37
REVIEW
Florence Chioma Mgbodile, Tochukwu Nwamaka T Nwagu
The current paper focuses on the impact of probiotics, African fermented foods and bioactive peptides on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection severity and related viral infections. Using probiotics or bioactive peptides as therapeutic adjuncts appears superior to standard care alone. Probiotics play critical roles in innate and adaptive immune modulation by balancing the gut microbiota to combat viral infections, secondary bacterial infections and microbial dysbiosis. African fermented foods contain abundant potential probiotic microorganisms such as the lactic acid bacteria (LAB), Saccharomyces, and Bacillus...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/36950261/a-straightforward-microfluidic-based-approach-toward-optimizing-transduction-efficiency-of-hiv-1-derived-lentiviral-vectors-in-bcp-all-cells
#38
JOURNAL ARTICLE
Seyed Esmaeil Ahmadi, Rima Manafi Shabestari, Amir Asri Kojabad, Majid Safa
BACKGROUND: HIV-1-derived lentiviral vectors (LVs) are capable of transducing human cells by integrating the transgene into the host genome. In order to do that, LVs should have enough time and space to interact with the surface of the target cells. Herein, we used a microfluidic system to facilitate the transduction of BCP-ALL cells. METHODS AND RESULTS: We used a SU-8 mold to fabricate a PDMS microfluidic chip containing three channels with a 50 μm height and a surface matching 96-well plates...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/36923508/overexpression-of-quorum-sensing-genes-in-acidithiobacillus-ferrooxidans-enhances-cell-attachment-and-covellite-bioleaching
#39
JOURNAL ARTICLE
Heejung Jung, Yuta Inaba, Alan C West, Scott Banta
Cell adhesion is generally a prerequisite to the microbial bioleaching of sulfide minerals, and surface biofilm formation is modulated via quorum sensing (QS) communication. We explored the impact of the overexpression of endogenous QS machinery on the covellite bioleaching capabilities of Acidithiobacillus ferrooxidans , a representative acidophilic chemolithoautotrophic bacterium. Cells were engineered to overexpress the endogenous qs-I operon or just the afeI gene under control of the tac promoter. Both strains exhibited increased transcriptional gene expression of afeI and improved cell adhesion to covellite, including increased production of extracellular polymeric substances and increased biofilm formation...
June 2023: Biotechnology Reports
https://read.qxmd.com/read/36915646/immunogenic-fusion-proteins-induce-neutralizing-sars-cov-2-antibodies-in-the-serum-and-milk-of-sheep
#40
JOURNAL ARTICLE
Gregory M Jacobson, Kirsty Kraakman, Olivia Wallace, Jolyn Pan, Alex Hennebry, Grant Smolenski, Ray Cursons, Steve Hodgkinson, Adele Williamson, William Kelton
Antigen-specific polyclonal immunoglobulins derived from the serum, colostrum, or milk of immunized ruminant animals have potential as scalable therapeutics for the control of viral diseases including COVID-19. Here we show that the immunization of sheep with fusions of the SARS-CoV-2 receptor binding domain (RBD) to ovine IgG2a Fc domains promotes significantly higher levels of antigen-specific antibodies compared to native RBD or full-length spike antigens. This antibody population contained elevated levels of neutralizing antibodies that suppressed binding between the RBD and hACE2 receptors in vitro...
June 2023: Biotechnology Reports
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