journal
https://read.qxmd.com/read/38606996/childhood-mycoplasma-pneumoniae-epidemiology-and-manifestation-in-northeast-and-inner-mongolia-china
#1
JOURNAL ARTICLE
Fei Wang, Qi Cheng, Hongying Duo, Jichun Wang, Jingjing Yang, Shujun Jing, Jing Li, Xiandong Zhou, Yunxiao Shang, Ning Chen, Zhiliang Tian, Han Zhang, Xuxu Cai
UNLABELLED: Mycoplasma pneumoniae (MP) is commonly detected in children. However, the epidemiological trends of MP in Northeast (NE) China are unclear. This retrospective study aimed to investigate the prevalence of MP infections in this understudied region. The clinical manifestations and bronchoscopic findings observed in hospitalized patients with severe Mycoplasma pneumoniae pneumonia (SMPP) were collected from comprehensive data obtained from six tertiary hospitals in NE and Inner Mongolian (IM) China, from 1 January 2017 to 31 December 2023...
April 12, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38606982/phylogenetic-analysis-of-the-promoter-element-2-of-paramyxo-and-filoviruses
#2
JOURNAL ARTICLE
Shoichi Ashida, Shohei Kojima, Takashi Okura, Fumihiro Kato, Wakako Furuyama, Shuzo Urata, Yusuke Matsumoto
UNLABELLED: Paramyxo- and filovirus genomes are equipped with bipartite promoters at their 3 ' ends to initiate RNA synthesis. The two elements, the primary promoter element 1 (PE1) and the secondary promoter element 2 (PE2), are separated by a spacer region that must be precisely a multiple of 6 nucleotides (nts), indicating these viruses adhere to the "rule of six." However, our knowledge of PE2 has been limited to a narrow spectrum of virus species. In this study, a comparative analysis of 1,647 paramyxoviral genomes from a public database revealed that the paramyxovirus PE2 can be clearly categorized into two distinct subcategories: one marked by C repeats at every six bases (exclusive to the subfamily Orthoparamyxovirinae ) and another characterized by CG repeats every 6 nts (observed in the subfamilies Avulavirinae and Rubulavirinae )...
April 12, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38606971/effect-of-hydration-on-urine-and-serum-histoplasma-antigen-levels-in-patients-with-disseminated-histoplasmosis
#3
LETTER
Michael Dzombo, Allyson L Hughes, Sara L Hollis, Christopher H Trabue, George E Nelson, Richard W Larue, Molly Busenbark, L Joseph Wheat, Melissa L Scalise
No abstract text is available yet for this article.
April 12, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38606959/planktonic-eukaryotes-in-the-chesapeake-bay-contrasting-responses-of-abundant-and-rare-taxa-to-estuarine-gradients
#4
JOURNAL ARTICLE
Hualong Wang, Feilong Liu, Min Wang, Yvan Bettarel, Yoanna Eissler, Feng Chen, Jinjun Kan
UNLABELLED: Phytoplankton are important drivers of aquatic ecosystem function and environmental health. Their community compositions and distributions are directly impacted by environmental processes and human activities, including in the largest estuary in North America, the Chesapeake Bay. It is crucial to uncover how planktonic eukaryotes play fundamental roles as primary producers and trophic links and sustain estuarine ecosystems. In this study, we investigated the detailed community structure and spatiotemporal variations of planktonic eukaryotes in the Chesapeake Bay across space and time for three consecutive years...
April 12, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38602399/comparison-of-targeted-next-generation-sequencing-and-the-xpert-mtb-rif-assay-for-detection-of-mycobacterium-tuberculosis-in-clinical-isolates-and-sputum-specimens
#5
JOURNAL ARTICLE
Hongtai Zhang, Xiaowei Dai, Peilei Hu, Lili Tian, Chuanyou Li, Beichuan Ding, Xinyu Yang, Xiaoxin He
Targeted next-generation sequencing (tNGS) can be used to perform Mycobacterium tuberculosis (MTB) complex-specific amplification or target capture directly from sputum samples, yielding simultaneous coverage of many genes and DNA regions associated with antimicrobial resistance (AMR). Performance comparisons of tNGS and another molecular testing tool, Xpert MTB/rifampicin (RIF), have been empirical. Here, using a dilution series of a RIF-resistant clinical isolate of MTB, we found that tNGS had a slightly lower limit of bacterial detection (102 CFU/mL) compared with Xpert MTB/RIF (103 CFU/mL) in culture medium...
April 11, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38602397/bacterial-and-fungal-community-structures-in-hulun-lake-are-regulated-by-both-stochastic-processes-and-environmental-factors
#6
JOURNAL ARTICLE
Yongquan Shang, Xibao Wang, Xiaoyang Wu, Huashan Dou, Qinguo Wei, Qi Wang, Gang Liu, Guolei Sun, Lidong Wang, Honghai Zhang
Microorganisms are a crucial component of lake ecosystems and significant contributors to biogeochemical cycles. However, the understanding of how primary microorganism groups (e.g., bacteria and fungi) are distributed and constructed within different lake habitats is lacking. We investigated the bacterial and fungal communities of Hulun Lake using high-throughput sequencing techniques targeting 16S rRNA and Internal Transcribed Spacer 2 genes, including a range of ecological and statistical methodologies. Our findings reveal that environmental factors have high spatial and temporal variability...
April 11, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38597637/whole-genome-sequencing-of-clinical-isolates-from-tuberculosis-patients-in-india-real-world-data-indicates-a-high-proportion-of-pre-xdr-cases
#7
JOURNAL ARTICLE
Aparna Bhanushali, Sachin Atre, Preethi Nair, Geethanjali Anilkumar Thandaseery, Sanchi Shah, Sanjana Kuruwa, Amrutraj Zade, Chaitali Nikam, Mangala Gomare, Anirvan Chatterjee
UNLABELLED: Treatment decisions for tuberculosis (TB) in the absence of full drug-susceptibility data can result in amplifying resistance and may compromise treatment outcomes. Genomics of Mycobacterium tuberculosis ( M.tb ) from clinical samples enables detection of drug resistance to multiple drugs. We performed whole-genome sequencing (WGS) for 600 clinical samples from patients with tuberculosis to identify the drug-resistance profile and mutation spectrum. We documented the reasons reported by clinicians for referral...
April 10, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38591917/tetracyclic-homoisoflavanoid-brazilin-a-natural-product-inhibits-c-di-amp-producing-enzyme-and-streptococcus-mutans-biofilms
#8
JOURNAL ARTICLE
Edwin M Rojas, Hua Zhang, Sadanandan E Velu, Hui Wu
The tenacious biofilms formed by Streptococcus mutans are resistant to conventional antibiotics and current treatments. There is a growing need for novel therapeutics that selectively inhibit S. mutans biofilms while preserving the normal oral microenvironment. Previous studies have shown that increased levels of cyclic di-AMP, an important secondary messenger synthesized by diadenylate cyclase (DAC), favored biofilm formation in S. mutans . Thus, targeting S. mutans DAC is a novel strategy to inhibit S. mutans biofilms...
April 9, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38587411/a-highly-conserved-trna-modification-contributes-to-c-albicans-filamentation-and-virulence
#9
JOURNAL ARTICLE
Bettina Böttcher, Sandra D Kienast, Johannes Leufken, Cristian Eggers, Puneet Sharma, Christine M Leufken, Bianka Morgner, Hannes C A Drexler, Daniela Schulz, Stefanie Allert, Ilse D Jacobsen, Slavena Vylkova, Sebastian A Leidel, Sascha Brunke
tRNA modifications play important roles in maintaining translation accuracy in all domains of life. Disruptions in the tRNA modification machinery, especially of the anticodon stem loop, can be lethal for many bacteria and lead to a broad range of phenotypes in baker's yeast. Very little is known about the function of tRNA modifications in host-pathogen interactions, where rapidly changing environments and stresses require fast adaptations. We found that two closely related fungal pathogens of humans, the highly pathogenic Candida albicans and its much less pathogenic sister species, Candida dubliniensis , differ in the function of a tRNA-modifying enzyme...
April 8, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38587390/outbreak-of-streptococcus-pyogenes-emm89-st646-in-a-head-and-neck-surgical-oncology-ward
#10
JOURNAL ARTICLE
Brian Hayama, Sohei Harada, Masahiro Suzuki, Yohei Doi, Yusuke Nomura, Kotaro Aoki, Kazumi Takehana, Tomomi Akatsuchi, Taisuke Enokida, Koichi Takeda, Akira Seto, Hiroki Mitani, Daisuke Ohkushi
UNLABELLED: Streptococcus pyogenes causes a variety of human infections, and hospital outbreaks with this pathogen have also been reported. The purpose of this study is to describe the clinical characteristics of an outbreak of S. pyogenes involving 15 patients and four healthcare workers (HCWs), as well as the molecular characteristics of the causative isolates. The course and response to the outbreak were reviewed, and information on the characteristics of the patients was extracted retrospectively from the medical records...
April 8, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38587385/the-role-of-composition-of-gut-microbiota-in-reflecting-the-severity-of-acute-pancreatitis-is-probably-overstated
#11
LETTER
Jia-Lin Wu, Jun-Yang Luo, Xin-Yi Deng, Tsz Yu Lau, Zai-Bo Jiang
No abstract text is available yet for this article.
April 8, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38578100/the-micronaut-am-antifungal-susceptibility-testing-method-does-not-overestimate-amphotericin-b-resistance-in-candida-auris
#12
LETTER
Paraskevi Mantzana, Efthymia Protonotariou, Georgios Meletis, Areti Tychala, Lemonia Skoura
No abstract text is available yet for this article.
April 5, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38578091/tracking-vibrio-population-dynamics-and-ecology-of-vibrio-parahaemolyticus-and-v-vulnificus-in-an-alabama-estuary
#13
JOURNAL ARTICLE
Blair H Morrison, Jessica L Jones, Brian Dzwonkowski, Jeffrey W Krause
UNLABELLED: Vibrio is a genus of halophilic, gram-negative bacteria found in estuaries around the globe. Integral parts of coastal cultures often involve contact with vectors of pathogenic Vibrio spp. (e.g., consuming raw shellfish). High rates of mortality from certain Vibrio spp. infections demonstrate the need for an improved understanding of Vibrio spp. dynamics in estuarine regions. Our study assessed meteorological, hydrographic, and biological correlates of Vibrio parahaemolyticus and V...
April 5, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38572997/refining-taxonomic-identification-of-microalgae-through-molecular-and-genetic-evolution-a-case-study-of-prorocentrum-lima-and-prorocentrum-arenarium
#14
JOURNAL ARTICLE
Danlin Zheng, Ligong Zou, Jian Zou, Qun Li, Songhui Lu
Species delimitation based on lineage definition has become increasingly popular. However, these methods have been limited, especially for species that lack genomic data and are morphologically similar. The trickiest part for the species identification is that the interspecific and intraspecific boundaries are vague. Taking Prorocentrum (Dinophyta) as an example, analysis of cell morphology, growth, and toxin synthesis in both species of P. lima and P. arenarium does not provide a reliable basis for species delineation...
April 4, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38572990/-sphingomonas-clade-and-functional-distribution-with-simulated-climate-change
#15
JOURNAL ARTICLE
Bahareh Sorouri, Nicholas C Scales, Brandon S Gaut, Steven D Allison
Microbes are essential for the functioning of all ecosystems, and as global warming and anthropogenic pollution threaten ecosystems, it is critical to understand how microbes respond to these changes. We investigated the climate response of Sphingomonas , a widespread gram-negative bacterial genus, during an 18-month microbial community reciprocal transplant experiment across a Southern California climate gradient. We hypothesized that after 18 months, the transplanted Sphingomonas clade and functional composition would correspond with site conditions and reflect the Sphingomonas composition of native communities...
April 4, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38572984/identification-of-kras-mutation-associated-gut-microbiota-in-colorectal-cancer-and-construction-of-predictive-machine-learning-model
#16
JOURNAL ARTICLE
Zigui Huang, Xiaoliang Huang, Yili Huang, Kunmei Liang, Lei Chen, Chuzhuo Zhong, Yingxin Chen, Chuanbin Chen, Zhen Wang, Fuhai He, Mingjian Qin, Chenyan Long, Binzhe Tang, Yongqi Huang, Yongzhi Wu, Xianwei Mo, Tang Weizhong, Jungang Liu
Gut microbiota has demonstrated an increasingly important role in the onset and development of colorectal cancer (CRC). Nonetheless, the association between gut microbiota and KRAS mutation in CRC remains enigmatic. We conducted 16S rRNA sequencing on stool samples from 94 CRC patients and employed the linear discriminant analysis effect size algorithm to identify distinct gut microbiota between KRAS mutant and KRAS wild-type CRC patients. Transcriptome sequencing data from nine CRC patients were transformed into a matrix of immune infiltrating cells, which was then utilized to explore KRAS mutation-associated biological functions, including Gene Ontology items and Kyoto Encyclopedia of Genes and Genomes pathways...
April 4, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38567975/taqman-pacman-a-simple-molecular-approach-for-positive-rapid-antigen-test-confirmation-during-periods-of-low-prevalence
#17
JOURNAL ARTICLE
Gregory R McCracken, Glenn Patriquin, Todd F Hatchette, Ross J Davidson, Barbara Goodall, Lisa Barrett, James MacDonald, Charles Heinstein, Janice Pettipas, John Ross, Jason J LeBlanc
UNLABELLED: Antigen-based rapid diagnostic tests (Ag-RDTs) were widely deployed to enhance SARS-CoV-2 testing capacity during the COVID-19 pandemic. Consistent with national guidance for low prevalence settings, positive Ag-RDTs were confirmed using nucleic acid amplification tests (NAATs) to avoid false positive results. However, increasing demands for positive Ag-RDT confirmation competed with other testing priorities in clinical laboratories. This work hypothesized that real-time RT-PCR without nucleic acid extraction (NAE) would be sufficiently sensitive to support positive Ag-RDT confirmation...
April 3, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38567974/arsenic-trioxide-impacts-hepatitis-b-virus-core-nuclear-localization-and-efficiently-interferes-with-hbv-infection
#18
JOURNAL ARTICLE
Samuel Hofmann, Julius Luther, Verena Plank, Andreas Oswald, Julia Mai, Ilka Simons, Julija Miller, Valeria Falcone, Lea Hansen-Palmus, Hartmut Hengel, Michael Nassal, Ulrike Protzer, Sabrina Schreiner
UNLABELLED: The key to a curative treatment of hepatitis B virus (HBV) infection is the eradication of the intranuclear episomal covalently closed circular DNA (cccDNA), the stable persistence reservoir of HBV. Currently, established therapies can only limit HBV replication but fail to tackle the cccDNA. Thus, novel therapeutic approaches toward curative treatment are urgently needed. Recent publications indicated a strong association between the HBV core protein SUMOylation and the association with promyelocytic leukemia nuclear bodies (PML-NBs) on relaxed circular DNA to cccDNA conversion...
April 3, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38567960/correction-for-choudhary-et-al-genome-wide-translational-response-of-candida-albicans-to-fluconazole-treatment
#19
JOURNAL ARTICLE
Saket Choudhary, Vasanthakrishna Mundodi, Andrew D Smith, David Kadosh
No abstract text is available yet for this article.
April 3, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38564670/-lactobacillus-rhamnosus-hn001-facilitates-the-efficacy-of-dual-pi3k-mtor-inhibition-prolonging-cardiac-transplant-survival-and-enhancing-antitumor-effect
#20
JOURNAL ARTICLE
Xiaolong Miao, Peng Jiang, Xiaotong Zhang, Xinqiang Li, Zelai Wu, Yuancong Jiang, Han Liu, Weixun Xie, Xinwei Li, Bingfeng Shi, Jinzhen Cai, Weihua Gong
UNLABELLED: Solid organ transplantation is a crucial treatment for patients who have reached the end stage of heart, lung, kidney, or liver failure. However, the likelihood of developing cancer post-transplantation increases. Additionally, primary malignant tumors remain a major obstacle to the long-term survival of transplanted organs. Therefore, it is essential to investigate effective therapies that can boost the immune system's ability to combat cancer and prevent allograft rejection...
April 2, 2024: Microbiology Spectrum
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