Johnathan A Khusid, Alex Vasquez, Areeba S Sadiq, Jennifer A Stockert, Blair Gallante, Alan Yaghoubian, Roman Shimonov, Aryeh Stock, William Atallah, Natasha Kyprianou, Wei Yang, Mantu Gupta
INTRODUCTION: Kidney stone matrix proteins may help explain cellular mechanisms of stone genesis. However, most existing proteomic studies have focused on calcium oxalate stones. Here, we present a comparative proteomic analysis of different kidney stone types. METHODS: Proteins were extracted from the stones of patients undergoing percutaneous nephrolithotomy (PCNL). Approximately 20 μg of protein was digested into tryptic peptides using filter aided sample preparation, followed by liquid chromatography tandem-mass-spectrometry using an EASY-nLC 1200 and Orbitrap Fusion Lumos mass spectrometer...
2023: American Journal of Clinical and Experimental Urology