journal
https://read.qxmd.com/read/28794987/whole-genome-of-klebsiella-aerogenes-px01-isolated-from-san-jacinto-river-sediment-west-of-baytown-texas-reveals-the-presence-of-multiple-antibiotic-resistance-determinants-and-mobile-genetic-elements
#21
JOURNAL ARTICLE
Rupa Iyer, Brian Iken, Ashish Damania
Klebsiella aerogenes is a Gram-negative bacterium of the family Enterobacteriaceae which is widely distributed in water, air and soil. It also forms part of the normal microbiota found in human and animal gastrointestinal tracts. Here we report the draft genome sequence (chromosome and 1 plasmid) of K. aerogenes strain PX01 compiled at the scaffold level from 97 contigs totaling 5,262,952 bp. K. aerogenes PX01 was isolated from sediment along the northern face of Burnet Bay west of Baytown, Texas. The nucleotide sequence of this genome was deposited into NCBI GenBank under the accession NJBB00000000...
December 2017: Genomics Data
https://read.qxmd.com/read/28761813/rna-seq-data-from-different-developmental-stages-of-rafflesia-cantleyi-floral-buds
#22
JOURNAL ARTICLE
Safoora Amini, Halimah Alias, Mohd Afiq Aizat-Juhari, Mohd-Noor Mat-Isa, Jumaat Haji Adam, Hoe-Han Goh, Kiew-Lian Wan
Rafflesia cantleyi, known as one of the world's largest flowers, is a specialised holoparasite due to dramatic morphological modifications. It possesses highly reduced vegetative structure and only appears as a flower for sexual reproduction. Moreover, it has an unusual life cycle in that its floral bud development takes up to nine months. In order to fully understand the highly modified floral organ structure and long life cycle of R. cantleyi, we used Illumina sequencing technology (HiSeq) for sequence generation followed by de novo assembly of sequence reads...
December 2017: Genomics Data
https://read.qxmd.com/read/28736703/genome-wide-mirna-gene-and-methylation-analysis-of-triple-negative-breast-cancer-to-identify-changes-associated-with-lymph-node-metastases
#23
JOURNAL ARTICLE
Kelly A Avery-Kiejda, Andrea Mathe, Rodney J Scott
Triple negative breast cancer (TNBC) is a particularly important breast cancer subtype with an aggressive clinical phenotype that is associated with a higher likelihood of metastasis. This subtype is characterized by an absence of the estrogen (ER) and progesterone (PR) receptors, as well as the human epidermal growth factor receptor 2 (HER2/HER neu). The absence of the three receptors significantly reduces targeted treatment options for patients with TNBC and as such, there is an urgent need to identify novel treatment targets...
December 2017: Genomics Data
https://read.qxmd.com/read/28736702/cultivar-specific-transcriptome-prediction-and-annotation-in-ficus-carica-l
#24
JOURNAL ARTICLE
Liceth Solorzano Zambrano, Gabriele Usai, Alberto Vangelisti, Flavia Mascagni, Tommaso Giordani, Rodolfo Bernardi, Andrea Cavallini, Riccardo Gucci, Giovanni Caruso, Claudio D'Onofrio, Mike Frank Quartacci, Piero Picciarelli, Barbara Conti, Andrea Lucchi, Lucia Natali
The availability of transcriptomic data sequence is a key step for functional genomics studies. Recently, a repertoire of predicted genes of a Japanese cultivar of fig ( Ficus carica L.) was released. Because of the great phenotypic variability that can be found in this species, we decided to study another fig genotype, the Italian cv. Dottato, in order to perform comparative studies between the two cultivars and extend the pan genome of this species. We isolated, sequenced and assembled fig genomic DNA from young fruits of cv...
September 2017: Genomics Data
https://read.qxmd.com/read/28736701/a-comparative-genome-analysis-of-cercospora-sojina-with-other-members-of-the-pathogen-genus-mycosphaerella-on-different-plant-hosts
#25
JOURNAL ARTICLE
Fanchang Zeng, Xin Lian, Guirong Zhang, Xiaoman Yu, Carl A Bradley, Ray Ming
Fungi are the causal agents of many of the world's most serious plant diseases causing disastrous consequences for large-scale agricultural production. Pathogenicity genomic basis is complex in fungi as multicellular eukaryotic pathogens. Here, we report the genome sequence of C. sojina , and comparative genome analysis with plant pathogen members of the genus Mycosphaerella ( Zymoseptoria. tritici (synonyms M. graminicola ), M. pini , M. populorum and M. fijiensis - pathogens of wheat, pine, poplar and banana, respectively)...
September 2017: Genomics Data
https://read.qxmd.com/read/28736700/profiling-and-characterization-of-a-longissimus-dorsi-muscle-microrna-dataset-from-an-f2-duroc%C3%A2-%C3%A3-%C3%A2-pietrain-pig-resource-population
#26
JOURNAL ARTICLE
Kaitlyn R Daza, Juan P Steibel, Deborah Velez-Irizarry, Nancy E Raney, Ronald O Bates, Catherine W Ernst
To elucidate the effects of microRNA (miRNA) regulation in skeletal muscle of adult pigs, miRNA expression profiling was performed with RNA extracted from longissimus dorsi (LD) muscle samples from 174 F2 pigs (~ 5.5 months of age) from a Duroc × Pietrain resource population. Total RNA was extracted from LD samples, and libraries were sequenced on an Illumina HiSeq 2500 platform in 1 × 50 bp format. After processing, 232,826,977 total reads were aligned to the Sus scrofa reference genome (v10.2.79), with 74...
September 2017: Genomics Data
https://read.qxmd.com/read/28725557/genome-assembly-of-chryseobacterium-sp-strain-ihbb-10212-from-glacier-top-surface-soil-in-the-indian-trans-himalayas-with-potential-for-hydrolytic-enzymes
#27
JOURNAL ARTICLE
Mohinder Pal, Mohit Kumar Swarnkar, Hena Dhar, Sanjay Chhibber, Arvind Gulati
The cold-active esterases are gaining importance due to their catalytic activities finding applications in chemical industry, food processes and detergent industry as additives, and organic synthesis of unstable compounds as catalysts. In the present study, the complete genome sequence of 4,843,645 bp with an average 34.08% G + C content and 4260 protein-coding genes are reported for the low temperature-active esterase-producing novel strain of Chrysobacterium isolated from the top-surface soil of a glacier in the cold deserts of the Indian trans-Himalayas...
September 2017: Genomics Data
https://read.qxmd.com/read/28725556/de-novo-transcriptome-assembly-for-the-lung-of-the-ornamented-pygmy-frog-microhyla-fissipes
#28
JOURNAL ARTICLE
Lusha Liu, Shouhong Wang, Lanying Zhao, Jianping Jiang
Microhyla fissipes, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. M. fissipes use of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in M. fissipes is very limited. In this study, we conducted de novo transcriptome assembly for the lung of M...
September 2017: Genomics Data
https://read.qxmd.com/read/28725555/genome-sequence-of-the-type-strain-clib-1764-t-%C3%A2-cbs-14374-t-of-the-yeast-species-kazachstania-saulgeensis-isolated-from-french-organic-sourdough
#29
JOURNAL ARTICLE
Véronique Sarilar, Lieven Sterck, Saki Matsumoto, Noémie Jacques, Cécile Neuvéglise, Colin R Tinsley, Delphine Sicard, Serge Casaregola
Kazachstania saulgeensis is a recently described species isolated from French organic sourdough. Here, we report the high quality genome sequence of a monosporic segregant of the type strain of this species, CLIB 1764(T) (= CBS 14374(T)). The genome has a total length of 12.9 Mb and contains 5326 putative protein-coding genes, excluding pseudogenes and transposons. The nucleotide sequences were deposited into the European Nucleotide Archive under the genome assembly accession numbers FXLY01000001-FXLY01000017...
September 2017: Genomics Data
https://read.qxmd.com/read/28721334/draft-genome-sequence-of-bradyrhizobium-paxllaeri-lmtr-21-t-isolated-from-lima-bean-phaseolus-lunatus-in-peru
#30
JOURNAL ARTICLE
Ernesto Ormeño-Orrillo, Luis Rey, David Durán, Carlos A Canchaya, Marco A Rogel, Doris Zúñiga-Dávila, Juan Imperial, Tomás Ruiz-Argüeso, Esperanza Martínez-Romero
Bradyrhizobium paxllaeri is a prevalent species in root nodules of the Lima bean (Phaseolus lunatus) in Peru. LMTR 21(T) is the type strain of the species and was isolated from a root nodule collected in an agricultural field in the Peruvian central coast. Its 8.29 Mbp genome encoded 7635 CDS, 71 tRNAs and 3 rRNAs genes. All genes required to stablish a nitrogen-fixing symbiosis with its host were present. The draft genome sequence and annotation have been deposited at GenBank under the accession number MAXB00000000...
September 2017: Genomics Data
https://read.qxmd.com/read/28702356/genome-sequence-of-bradyrhizobium-sp-lmtr-3-a-diazotrophic-symbiont-of-lima-bean-phaseolus-lunatus
#31
JOURNAL ARTICLE
Ernesto Ormeño-Orrillo, Luis Rey, David Durán, Carlos A Canchaya, Doris Zúñiga-Dávila, Juan Imperial, Esperanza Martínez-Romero, Tomás Ruiz-Argüeso
Bradyrhizobium sp. LMTR 3 is a representative strain of one of the geno(species) of diazotrophic symbionts associated with Lima bean (Phaseolus lunatus) in Peru. Its 7.83 Mb genome was sequenced using the Illumina technology and found to encode a complete set of genes required for nodulation and nitrogen fixation, and additional genes putatively involved in root colonization. Its draft genome sequence and annotation have been deposited at GenBank under the accession number MAXC00000000.
September 2017: Genomics Data
https://read.qxmd.com/read/28702355/whole-genome-sequencing-and-annotation-of-halophilic-salinicoccus-sp-bab-3246-isolated-from-the-coastal-region-of-gujarat
#32
JOURNAL ARTICLE
Vishal Mevada, Shradhdha Patel, Jignesh Pandya, Himani Joshi, Rajesh Patel
Salinicoccus sp. BAB 3246 is a halophilic bacterium isolated from a marine water sample collected from the coastal region of Gujarat, India, from a surface water stream. Based on 16sRNA sequencing, the organism was identified as Salinicoccus sp. BAB 3246 (Genebank ID: KF889285). The present work was performed to determine the whole genome sequence of the organism using Ion Torrent PGM platform followed by assembly using the CLC genomics workbench and genome annotation using RAST, BASys and MaGe. The complete genome sequence was 713,204 bp identified by with second largest size for Salinicoccus sp...
September 2017: Genomics Data
https://read.qxmd.com/read/28664146/oviductal-transcriptional-profiling-of-a-bovine-fertility-model-by-next-generation-sequencing
#33
JOURNAL ARTICLE
Angela Maria Gonella-Diaza, Sónia Cristina da Silva Andrade, Mariana Sponchiado, Guilherme Pugliesi, Fernando Silveira Mesquita, Veerle Van Hoeck, Ricardo de Francisco Strefezzi, Gustavo R Gasparin, Luiz L Coutinho, Mario Binelli
In cattle, the oviduct plays a fundamental role in the reproductive process. Oviductal functions are controlled by the ovarian sex steroids: estradiol and progesterone. Here, we tested the hypothesis that the exposure to contrasting sex steroid milieus differentially impacts the oviductal transcriptional profile. We manipulated growth of the pre-ovulatory follicle to obtain cows that ovulated a larger (LF group) or a smaller (SF group) follicle. The LF group presented greater proestrus/estrus concentrations of estradiol and metaestrus concentrations of progesterone (Gonella-Diaza et al...
September 2017: Genomics Data
https://read.qxmd.com/read/28652975/draft-genome-sequence-of-massilia-sp-kim-isolated-from-south-african-grassland-biome-soils
#34
JOURNAL ARTICLE
Surendra Vikram, Nerissa Govender, Mubanga Helen Kabwe, Oliver Bezuidt, Thulani P Makhalanyane
Massilia sp. are aerobic, Gram-negative, rod-shaped bacteria that are found in air, water, and soils. Here we describe the draft genome sequence of Massilia sp. KIM, isolated from the South African grassland soils. The total length of the genome was estimated at 5.73 Mb, comprised of 17 contigs. The draft genome has been deposited in the DDBJ/EMBL/GenBank under the accession MVAD10000000 and is available for download at: https://www.ncbi.nlm.nih.gov/nuccore/MVAD00000000. Additionally, the raw short reads are available in the NCBI SRA database under the accession number: SRR5469241...
September 2017: Genomics Data
https://read.qxmd.com/read/28652974/the-draft-genome-sequence-of-hymenobacter-sp-cra2-isolated-from-nama-karoo-shrub-land-soils-from-south-africa
#35
JOURNAL ARTICLE
Mubanga H Kabwe, Nerissa Govender, Surendra Vikram, Oliver M Bezuidt, Thulani P Makhalanyane
Here we report the draft genome sequence of Hymenobacter sp. CRA2 isolated from the Nama Karoo shrub land soils of the Northern Cape, South Africa. This genome is approximately 5.88 Mb long and the assembly comprised 45 contigs. The draft genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession number NZ_MVBC00000000 and is available for download at: https://www.ncbi.nlm.nih.gov/nuccore/NZ_MVBC00000000.1.
September 2017: Genomics Data
https://read.qxmd.com/read/28649496/de-novo-transcriptome-assembly-of-loggerhead-sea-turtle-nesting-of-the-colombian-caribbean
#36
JOURNAL ARTICLE
Javier Hernández-Fernández, Andrés Pinzón, Leonardo Mariño-Ramírez
Loggerhead sea turtle Caretta caretta is widely distributed in the oceans of tropical and subtropical latitude. This turtle is an endangered species due to anthropic and natural factors that have decreased their population levels. In this study, RNA sequencing and de-novo assembly of genes expressed in blood were performed. The raw FASTQ files have been deposited on NCBI's SRA database with accession number SRX2629512. A total of 5.4 Gb raw sequence data were obtained, corresponding to 48,257,019 raw reads...
September 2017: Genomics Data
https://read.qxmd.com/read/28626638/transcriptome-of-barley-under-three-different-heavy-metal-stress-reaction
#37
JOURNAL ARTICLE
Martina Kintlová, Nicolas Blavet, Radim Cegan, Roman Hobza
In the present study, we used Illumina sequencing technology (HiSeq 2000) to sequence the transcriptome of barley (Hordeum vulgare L., cv. Morex) under three different heavy metal stress conditions: copper, zinc and cadmium. For each of those metals, the concentration causing a 50% inhibitory effect for root growth (EC50) was determined. We sequenced the total RNA of both roots and shoots from barley with and without heavy metal treatments in three replicates. Raw reads of the transcriptome project have been deposited in NCBI's BioProject accession number PRJNA382490...
September 2017: Genomics Data
https://read.qxmd.com/read/28626637/transcriptome-analysis-of-reproductive-tissue-differentiation-in-jatropha-curcas-linn
#38
JOURNAL ARTICLE
Nisha Govender, Siju Senan, Zeti-Azura Mohamed-Hussein, Wickneswari Ratnam
Shoot and inflorescence are central physiological and developmental tissues of plants. Flowering is one of the most important agronomic traits for improvement of crop yield. To analyze the vegetative to reproductive tissue transition in Jatropha curcas, gene expression profiles were generated from shoot and inflorescence tissues. RNA isolated from both tissues was sequenced using the Ilumina HiSeq 2500 platform. Differential gene expression analysis identified key biological processes associated with vegetative to reproductive tissue transition...
September 2017: Genomics Data
https://read.qxmd.com/read/28580299/whole-genome-shotgun-sequence-of-phenazine-producing-endophytic-streptomyces-kebangsaanensis-suk12
#39
JOURNAL ARTICLE
Juwairiah Remali, Kok-Keong Loke, Chyan Leong Ng, Wan Mohd Aizat, John Tiong, Noraziah Mohamad Zin
Streptomyces sp. produces bioactive compounds with a broad spectrum of activities. Streptomyces kebangsaanesis SUK12 has been identified as a novel endophytic bacteria isolated from ethnomedicinal plant Portulaca olerace, and was found to produce the phenazine class of biologically active antimicrobial metabolites. The potential use of the phenazines has led to our research interest in determining the genome sequence of Streptomyces kebangsaanensis SUK12. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number PRJNA269542...
September 2017: Genomics Data
https://read.qxmd.com/read/28560170/rna-seq-detects-pharmacological-inhibition-of-epstein-barr-virus-late-transcription-during-spontaneous-reactivation
#40
JOURNAL ARTICLE
An T Phan, Delsy M Martinez, Jj L Miranda
The stepwise and sequential expression of viral genes underlies progression of the infectious life cycle. The Epstein-Barr virus (EBV) is both a tractable model for elucidating principles of transcription as well as a global health threat. We describe an experimental protocol and bioinformatics pipeline for functional identification of EBV true late genes, the last step of transcription prior to virion packaging and egress. All data have been uploaded to the Gene Expression Omnibus under accession code GSE96689...
September 2017: Genomics Data
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