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Agnès Garcias López, Louise Brogaard, Peter Mh Heegaard, Susanna Cirera, Kerstin Skovgaard
BACKGROUND: MicroRNAs (miRNAs) are short non-coding RNA molecules which regulate gene expression post-transcriptionally and are involved in a multitude of cellular processes. MiRNAs are known to be very stable compared to messenger RNAs (mRNAs), making them excellent candidates as biomarkers for disease. Recently, studies have suggested that miRNA stability in formalin fixed samples might dependend on their nucleotide composition. OBJECTIVE: To explore the stability of a panel of miRNAs isolated from porcine blood and lung tissue after heat and enzyme treatment...
January 30, 2019: MicroRNA
A Marchand, I Roulland, F Semence, K Schröder, V Domergue, M Audran
BACKGROUND: The recent development of drugs that stabilize HIFalpha, called HIF stabilizers, offers a new strategy for treating anemia. Although these drugs are still in clinical trials, misuse for doping has already begun. Identifying the biomarkers of HIF stabilizers would therefore help in detecting this drug misuse by athletes. OBJECTIVE: Our aim was twofold: to determine whether hypoxamiRs, the microRNAs associated with the cellular response to hypoxia, are potential biomarkers of HIF stabilizers in blood and whether the response to treatment with an HIF stabilizer differs from the response to a hypoxic environment...
January 17, 2019: MicroRNA
Helal F Hetta, Asmaa M Zahran, Engy A Shafik, Reham I El-Mahdy, Nahed A Mohamed, Emad Eldin Nabil, Hend M Esmaeel, Ola A Alkady, Azza Elkady, Dina A Mohareb, Amal Hosni, Mohammed Mahmoud Mostafa, Abeer Elkady
BACKGROUND AND AIM: Lung cancer (LC) is a major cancer killer worldwide, and 5-yr survival is extremely poor (≤15%), accentuating the need for more effective diagnostic and therapeutic strategies. Studies have shown cell-free microRNAs (miRNAs) circulating in the serum and plasma with specific expression in cancer, indicating the potential of using miRNAs as biomarkers for cancer diagnosis and therapy. This study aimed to identify differentially-expressed two miRNAs in the plasma of non-small cell lung cancer (NSCLC) patients that might be a clinically useful tool for lung cancer early detection...
January 15, 2019: MicroRNA
Fathelrahman M Hassan
INTRODUCTION: Association studies with factor candidates have advised that single nucleotide polymorphisms (SNPs) could also be relating to CML progression and to the response to medical care. Genetic variation in miR-1206 of both derived and neighborhood SNPs process genes will contribute to the predisposition for cancer. The role of those within the risk of CML has not been extensively studied. Therefore, the aim of this study was to evaluate whether polymorphisms in rs2114358 in pre-miRNAs process genes contribute to the risk of CML...
January 2, 2019: MicroRNA
Sunny Yadav, Devashree Jahagirdar, Mamta Shekhawat, Nilesh Kumar Sharma
INTRODUCTION: In cancer therapeutics, several new classes of small molecules based targeted drug options are reported including peptide mimetic and small RNAs therapeutics. Small RNAs represent a class of short non-coding endogenous RNAs that plays an important role in transcriptional and post transcriptional gene regulation among varied type of species including plants and animals. METHODS: To address the role of small RNAs from plant sources upon cancer cells, authors report on effects of small RNAs fraction of potato in vitro model of human derived HeLa cancer cells...
December 17, 2018: MicroRNA
Rashid Mir, Chandan K Jha, Imadeldin Elfaki, Suriya Rehman, Jamsheed Javid, Naina Khullar, Shaheena Banu, S M S Chahal
AIM: The microRNAs regulate expression of multiple genes involved in diseases such as cancer, diabetes and cardiovascular disease. In this study we have investigated the association between the miR-224 gene polymorphism (rs188519172A>G) and susceptibility of coronary artery disease CAD. METHODOLOGY: Hundred CAD patients and 100-matched healthy control were included. Genotyping of the miR-224 (rs188519172A>G) polymorphism was performed using Amplification refractory mutation system PCR method (ARMS-PCR)...
December 11, 2018: MicroRNA
Evita Lindholm, Suvi-Katri Leivonen, Eldri Undlien, Daniel Nebdal, Anna Git, Carlos Caldas, Anne-Lise Børresen-Dale, kristine K. Sahlberg
HER2 positive breast cancers (BC) have aggressive behavior and poor prognosis. Previously, we have identified miR-342-5p as an upstream regulator of HER2 signaling, as well as inhibitor of HER2 positive BC cell line growth. Here, we aimed to further investigate the molecular mechanisms behind miR-342-5p-induced HER2 pathway deregulation. Two HER2 amplified breast cancer cell lines were transiently transfected with miR-342-5p mimic or negative control, and gene expression was analyzed by Agilent microarrays...
December 6, 2018: MicroRNA
Abdolkarim Moazeni-Roodi, Saeid Ghavami, Mohammad Hashemi
Growing evidence propose an association between miRNA polymorphisms and cancer susceptibility. This study aimed to examine the impact of miR-605 rs2043556 polymorphism on cancer risk through a meta-analysis based on 3198 cancer cases and 4943 controls. Eligible studies were retrieved by searching Web of Science, PubMed, Scopus, and Google scholar databases up to August 27, 2018. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated using a random-effect model to estimate the strength of association between rs2043556 variant of miR-605 and cancer risk...
December 3, 2018: MicroRNA
C Simon Durai Raj, Krishnaraj Thirugnanasambantham, Mohamed Ibrahim Hairul Islam, S Mathan Kumar, Sandhya Sundaram, Ganapathy Ashok, Venugopal Senthilkumar, Senguttuvan Muralidaran, S Saravanan
BACKGROUND: Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease leading to bones and joints destruction. It is one of the major causes of lifetime disability and mortality among humans in the developing and developed countries. It was evident that epigenetic dysregulation is related to the pathogenesis of RA. MicroRNAs (miRNAs) are small non-coding RNAs that are epigenetic regulators for diverse biological processes and also provided novel molecular insights in the formation of arthritis...
December 3, 2018: MicroRNA
Claudia Lang, Sakuntala Karunairetnam, Kim R Lo, Andrew V Kralicek, Ross N Crowhurst, Andrew P Gleave, Robin M MacDiarmid, John R Ingram
BACKGROUND: The discovery that a plant microRNA (miRNAs) from rice (Oryza sativa miR168a) can modify post-transcriptional expression of the mammalian Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) gene highlights the potential for cross-kingdom miRNA-mRNA interactions. OBJECTIVE: To investigate whether common variants of the conserved miR168a family have the capability for similar cross-kingdom regulatory functions, we selected sequences from three dietary plant sources: rice (Oryza sativa), tomato (Solanum lycopersicum), apple (Malus domestica) and compared their ability to regulate human LDLRAP1 expression...
December 2, 2018: MicroRNA
Roberto Cannataro, Mariarita Perri, Luca Gallelli, Maria Cristina Caroleo, Giovambattista De Sarro, Erika Cione
BACKGROUND: The ketogenic diet (KD) promotes metabolic changes and optimizes energy metabolism. It is unknown if microRNAs (miRs) are influenced by KD in obese subjects. OBJECTIVES: The aim of this study was to evaluate 6 weeks of biphasic KD in obese subjects. We enrolled 36 obese subjects (18 females and 18 males) in stage 1 of Edmonton Obesity Staging System (EOSS) parameter. Blood biochemical parameters were dosed by ADVIA 1800. RESULT: The screening of circulating miRs was performed with the FDA approved platform n-counter flex...
November 25, 2018: MicroRNA
Alberto Izzotti
No abstract text is available yet for this article.
2019: MicroRNA
Elton Amp Dudink, Barend W Florijn, Bob Weijs, Jacques Mgj Duijs, Justin Glm Luermans, Frederique Ecm Peeters, Leon J Schurgers, Joachim E Wildberger, Uli Schotten, Roel Bijkerk, Harry Jgm Crijns, Anton Jan van Zonneveld
Background Atrial fibrillation (AF) in patients without concomitant cardiovascular pathophysiological disease, is called idiopathic atrial fibrillation (iAF). Nonetheless, iAF patients have oftentimes subclinical coronary (micro)vascular dysfunction and, particularly in women, a higher prevalence of subsequent cardiovascular comorbidities. Previously, we identified a plasma miRNA association with diabetes and microvascular injury in diabetic nephropathy (DN) patients. Therefore, in this study we assessed whether plasma levels of these diabetic, microvascular injury associated miRNAs reflect microvascular integrity in iAF patients, associate with the presence of paroxysmal arrhythmia or instead are determined by concealed coronary artery disease...
November 22, 2018: MicroRNA
Anjana Rajendiran, Saravanan Vijayakumar, Archana Pan
BACKGROUND: MicroRNAs (miRNAs) are a class of small non-coding, endogenous RNAs that regulate gene expression at post-transcriptional level. In plants, miRNAs are usually of 18-24 nucleotide in length and play humongous role by aiding in development, growth, defense, biotic and abiotic stress responses etc. Objective: Arachis hypogaea is an economically important oil seed crop and human dietary source cultivated mostly in tropical and subtropical regions. In the present study, an initiative was taken to uncover miRNAs, targets and their functions in this important plant species...
November 4, 2018: MicroRNA
Aniello Russo, Nicoletta Potenza
BACKGROUND: MicroRNA-125a is present in all animals with bilateral symmetry and displays a conserved nucleotide sequence with a section of 11 bases including the seed region that is identical in all considered species. It primarily downregulates the expression of LIN28, thereby promoting cell differentiation and larval phase transitions in nematodes, mammals and insects. OBJECTIVE: In this review we focus on the cellular control of miR-125a expression and its antiproliferative activity...
November 4, 2018: MicroRNA
Emmanuel N Kontomanolis, Zacharias Fasoulakis, Valentinos Papamanolis, Sofia Koliantzaki, Georgios Dimopoulos, Nikolaos J Kambas
OBJECTIVE To review the recent data considering the expression profile and the role of microRNAs in breast tumorigenesis, and their impact on -the vital for breast cancer progression- angiogenesis. METHODS PubMed was searched for studies focused on data that associate microRNA with breast cancer, using the terms ''breast", "mammary gland", "neoplasia'', "angiogenesis" and ''microRNA'' between 1997-2018. RESULTS Aberrant expression of several circulating and tissue miRNAs is observed in human breast neoplasms with the deregulation of several miRNAs having a major participation in breast cancer progression...
October 17, 2018: MicroRNA
Sara Zamani, Amir Sohrabi, Seyed Masoud Hosseini, Marjan Rahnamaye-Farzami, Abolfazl Akbari
BACKGROUND: Early diagnosis is an important factor to improve survival of invasive cervical cancer (ICC) patients. Molecular biomarkers such as micro RNA (miRNA) can be used in the early detection of ICC. The expression of miR-21 and miR-29a are deregulated in many types of human cancers. OBJECTIVE: The aim of this study was to investigate the differences in miR-21 and miR-29a expression patterns in Human Papilloma Virus (HPV) infection and various grades of cervical cancer among Iranian women...
October 17, 2018: MicroRNA
D Andrei, R A Nagy, A P A van Montfoort, U J F Tietge, M Terpstra, K Kok, A van den Berg, A Hoek, J Kluiver, R B Donker
BACKGROUND: mural granulosa cells (MGCs) and cumulus cells (CCs) are two specialized cell types that differentiate from a common progenitor during folliculogenesis. Although these two cell types have specialized functions and gene expression profiles, little is known about their microRNA (miRNA) expression patterns. OBJECTIVE: to describe the miRNA profile of mural and cumulus granulosa cells from human pre-ovulatory follicles Methods: using small RNA sequencing, we definedinvestigated the miRNA expression profiles of human primary MGCs and CCs, isolated from healthy women undergoing ovum pick-up for in vitro fertilization (IVF)...
September 12, 2018: MicroRNA
Majid Tafrihi, Elham Hasheminasab
INTRODUCTION: MicroRNAs (miRNAs), which are evolutionarily conserved, and endogenous non-coding RNAs, participate in the post-transcriptional regulation of eukaryotic genes. The biogenesis of miRNAs occurs in the nucleus. Then, in the cytoplasm, they are assembled along with some proteins in a ribonucleoprotein complex called RISC. miRNA component of the RISC complex binds to the complementary sequence of mRNA target depending on the degree of complementarity, and leads to mRNA degradation and/or inhibition of protein synthesis...
August 26, 2018: MicroRNA
Sybille Krauss, Rohit Nalavade, Stephanie Weber, Katlynn Carter, Bernd O Evert
Background: Spinocerebellar ataxia type 3 (SCA3), the most common spinocerebellar ataxia, is caused by a polyglutamine (polyQ) expansion in the protein ataxin-3 (ATXN3). Silencing the expression of polyQ-expanded ATXN3 rescues the cellular disease phenotype. Objective: This study investigated the differential expression of microRNAs (miRNAs), small noncoding RNAs targeting gene expression, in lymphoblastoid cells (LCs) from SCA3 patients and the capability of identified deregulated miRNAs to target and alter ATXN3 expression...
August 21, 2018: MicroRNA
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