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International Journal of Stem Cells

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https://read.qxmd.com/read/31023003/enhanced-anti-cancer-effects-of-conditioned-medium-from-hypoxic-human-umbilical-cord-derived-mesenchymal-stem-cells
#1
Kyu-Hyun Han, Ae-Kyeong Kim, Gun-Jae Jeong, Hye Ran Jeon, Suk Ho Bhang, Dong-Ik Kim
Background and Objectives: There have been contradictory reports on the pro-cancer or anti-cancer effects of mesenchymal stem cells. In this study, we investigated whether conditioned medium (CM) from hypoxic human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) (H-CM) showed enhanced anti-cancer effects compared with CM from normoxic hUC-MSCs (N-CM). Methods and Results: Compared with N-CM, H-CM not only strongly reduced cell viability and increased apoptosis of human cervical cancer cells (HeLa cells), but also increased caspase-3/7 activity, decreased mitochondrial membrane potential (MMP), and induced cell cycle arrest...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31023002/characterization-and-differentiation-of-circulating-blood-mesenchymal-stem-cells-and-the-role-of-phosphatidylinositol-3-kinase-in-modulating-the-adhesion
#2
Yoon-Kyung Park, Seong-Joo Heo, Jai-Young Koak, Gang-Seok Park, Tae-Jun Cho, Seong-Kyun Kim, Jaejin Cho
Bone marrow mesenchymal stem cells (BM MSCs) can differentiate into multi-lineage tissues. However, obtaining BM MSCs by aspiration is difficult and can be painful; therefore peripheral blood (PB) MSCs might provide an easier alternative for clinical applications. Here, we show that circulating PB MSCs proliferate as efficiently as BM MSCs in the presence of extracellular matrix (ECM) and that differentiation potential into osteoblast in vitro and in vivo . Both BM MSCs and PB MSCs developed into new bone when subcutaneously transplanted into immune-compromised mice using hydroxyapatite/tricalcium phosphate as a carrier...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31023001/angiotensin-ii-and-tgf-%C3%AE-1-induce-alterations-in-human-amniotic-fluid-derived-mesenchymal-stem-cells-leading-to-cardiomyogenic-differentiation-initiation
#3
Monika Gasiūnienė, Gintautas Petkus, Dalius Matuzevičius, Dalius Navakauskas, Rūta Navakauskienė
Background and Objectives: Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) may be a valuable source for cardiovascular tissue engineering and cell therapy. The aim of this study is to verify angiotensin II and transforming growth factor-beta 1 (TGF- β 1) as potential cardiomyogenic differentiation inducers of AF-MSCs. Methods and Results: AF-MSCs were obtained from amniocentesis samples from second-trimester pregnant women, isolated and characterized by the expression of cell surface markers (CD44, CD90, CD105 positive; CD34 negative) and pluripotency genes (OCT4, SOX2, NANOG, REX1)...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31023000/hybrid-nanofiber-scaffold-based-direct-conversion-of-neural-precursor-cells-dopamine-neurons
#4
Mi-Sun Lim, Seung Hwan Ko, Min Sung Kim, Byungjun Lee, Ho-Sup Jung, Keesung Kim, Chang-Hwan Park
The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The generation and maturation of iDA neurons (microtubule-associated protein 2-positive and tyrosine hydroxylase-positive) and iNPCs (NESTIN-positive and SOX2-positive) increased on fiber and dot scaffolds as compared to that of the flat (control) scaffold...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31022999/adipose-tissue-derived-stem-cell-therapy-for-cavernous-nerve-injury-induced-erectile-dysfunction-in-the-rat-model-a-systematic-review-and-meta-analysis-using-methodological-quality-assessment
#5
Hyo Jung Park, Hyunsuk Jeong, Yong Hyun Park, Hyeon Woo Yim, U-Syn Ha, Sung-Hoo Hong, Sae Woong Kim, Na Jin Kim, Ji Youl Lee
Background and Objectives: Few studies were evaluated the effect of blindness on outcome in animal models, though a potential effect of blinding has been reported in clinical trials. We evaluated the effects of adipose tissue-derived stem cells (ADSCs) on cavernous nerve injury (CNI)-induced erectile dysfunction (ED) in the rat and examined how proper blinding of the outcome assessor affected treatment effect. Methods and Results: We searched in Pubmed, EMBASE, Cochrane and Web of Science databases from inception to January 2019...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31022998/effect-of-bone-marrow-derived-mesenchymal-stem-cells-on-ischaemic-reperfused-hearts-in-adult-rats-with-established-chronic-kidney-disease
#6
Gehane M Hamed, Wessam E Morsy, Manal S Abd-El Hamid, Arwa Abd El Moniem Hassan, Fatma A Abu Zahra
Background and Objectives: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are adult multipotent non-haematopoietic stem cells that have regeneration potential. The current study aimed to detect the ability of BM-MSCs to improve kidney and cardiac functions in adult rats with established chronic kidney disease. Methods: Rats were divided into sham-operated control, untreated sub totally nephrectomised and treated sub totally nephrectomised groups. Body weight, kidney and cardiac tissue weights, plasma creatinine and urea levels and arterial blood pressure were measured...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31022997/efficacies-of-stem-cell-therapies-for-functional-improvement-of-the-%C3%AE-cell-in-patients-with-diabetes-a-systematic-review-of-controlled-clinical-trials
#7
Gyudeok Hwang, Hyunsuk Jeong, Hae Kyung Yang, Hun-Sung Kim, Hanter Hong, Na Jin Kim, Il-Hoan Oh, Hyeon Woo Yim
Background and Objectives: This study was performed to investigate whether stem cell therapy enhances β cell function by meta-analysis with proper consideration of variability of outcome measurements in controlled trial of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) patients. Methods: A systematic search was performed from inception to January 2018 in PubMed, EMBASE, and Cochrane databases. β cell function was assessed by stimulated C-peptide, fasting C-peptide, normal glycosylated hemoglobin levels (HbA1C), and exogenous insulin dose patterns...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31022996/regenerative-medicine-of-the-bile-duct-beyond-the-myth
#8
Elina Maria Buisson, Jaemin Jeong, Han Joon Kim, Dongho Choi
Cholangiopathies are rare diseases of the bile duct with high mortality rates. The current treatment for cholangiopathies is liver transplantation, but there are significant obstacles including a shortage of donors and a high risk of complications. Currently, there is only one available medicine on the market targeting cholangiopathies, and the results have been inadequate in clinical therapy. To overcome these obstacles, many researchers have used human induced pluripotent stem cells (hPSC) as a source for cholangiocyte-like cell generation and have incorporated advances in bioprinting to create artificial bile ducts for implantation and transplantation...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31022995/fgf-17-from-hypoxic-human-wharton%C3%A2-s-jelly-derived-mesenchymal-stem-cells-is-responsible-for-maintenance-of-cell-proliferation-at-late-passages
#9
Kyu-Hyun Han, Min-Hee Kim, Gun-Jae Jeong, Ae-Kyeong Kim, Jong Wook Chang, Dong-Ik Kim
Background and Objectives: Although it is well known that hypoxic culture conditions enhance proliferation of human mesenchymal stem cells, the exact mechanism is not fully understood. In this study, we investigated the effect of fibroblast growth factor (FGF)-17 from hypoxic human Wharton’s Jelly-derived mesenchymal stem cells (hWJ-MSCs) on cell proliferation at late passages. Methods and Results: hWJ-MSCs were cultured in α -MEM medium supplemented with 10% fetal bovine serum (FBS) in normoxic (21% O2 ) and hypoxic (1% O2 ) conditions...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/31022994/mesenchymal-stem-cells-from-the-wharton%C3%A2-s-jelly-of-the-human-umbilical-cord-biological-properties-and-therapeutic-potential
#10
Luigi Marino, Maria Antonietta Castaldi, Rosa Rosamilio, Enrico Ragni, Rosa Vitolo, Caterina Fulgione, Salvatore Giovanni Castaldi, Bianca Serio, Rosario Bianco, Maurizio Guida, Carmine Selleri
Wharton’s jelly mesenchymal stem cells (WJ-MSCs) are a class of stem cells with high differentiative potential, an immuno-privileged status and easy access for collection, which raise no legal or ethical issues. WJ-MSCs exhibit several features of embryonic stem cells, both in the phenotypic and genetic aspects, with only a few differences, such as a shorter doubling time and a more extensive ex vivo expansion capacity. WJ-MSCs have immunomodulatory properties, involving both innate and adaptive immune responses...
April 30, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836735/mbp-fgf2-immobilized-matrix-maintains-self-renewal-and-myogenic-differentiation-potential-of-skeletal-muscle-stem-cells
#11
Jay Prakash Sah, Nguyen Thi Thu Hao, Yunhye Kim, Tamar Eigler, Eldad Tzahor, Sang-Heon Kim, Yongsung Hwang, Jeong Kyo Yoon
The robust capacity of skeletal muscle stem cells (SkMSCs, or satellite cells) to regenerate into new muscles in vivo has offered promising therapeutic options for the treatment of degenerative muscle diseases. However, the practical use of SkMSCs to treat muscle diseases is limited, owing to their inability to expand in vitro under defined cultivation conditions without loss of engraftment efficiency. To develop an optimal cultivation condition for SkMSCs, we investigated the behavior of SkMSCs on synthetic maltose-binding protein (MBP)-fibroblast growth factor 2 (FGF2)-immobilized matrix in vitro ...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836734/role-of-hif1-%C3%AE-regulatory-factors-in-stem-cells
#12
REVIEW
Hyun Jik Lee, Young Hyun Jung, Gee Euhn Choi, Jun Sung Kim, Chang Woo Chae, Ho Jae Han
Hypoxia-inducible factor 1 (HIF1) is a master transcription factor that induces the transcription of genes involved in the metabolism and behavior of stem cells. HIF1-mediated adaptation to hypoxia is required to maintain the pluripotency and survival of stem cells under hypoxic conditions. HIF1 activity is well known to be tightly controlled by the alpha subunit of HIF1 (HIF1 α ). Understanding the regulatory mechanisms that control HIF1 activity in stem cells will provide novel insights into stem cell biology under hypoxia...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836732/toward-a-reconceptualization-of-stem-cells-from-cellular-plasticity
#13
REVIEW
Tao Liu, Li Chen, Zhongjian Zhao, Shichang Zhang
The slow progress in clinical applications of stem cells and the bewildering mechanisms involved have puzzled many researchers. Recently, the increasing evidences have indicated that cells have superior plasticity in vivo or in vitro, spontaneously or under extrinsic specific inducers. The concept of stem cells may be challenged, or even replaced by the concept of cell plasticity when cell reprogramming technology is progressing rapidly. The characteristics of stem cells are manifestations of cellular plasticity...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836731/tgf-%C3%AE-signalling-is-suppressed-under-pro-hypertrophic-conditions-in-msc-chondrogenesis-due-to-tgf-%C3%AE-receptor-downregulation
#14
Christian G Pfeifer, Alexandra Karl, Maximilian Kerschbaum, Arne Berner, Siegmund Lang, Rupert Schupfner, Matthias Koch, Peter Angele, Michael Nerlich, Michael B Mueller
Background and Objectives: Mesenchymal stem cells (MSCs) become hypertrophic in long term despite chondrogenic differentiation following the pathway of growth plate chondrocytes. This terminal differentiation leads to phenotypically unstable cartilage and was mirrored in vitro by addition of hypertrophy inducing medium. We investigated how intrinsic TGF- β signaling is altered in pro-hypertrophic conditions. Methods and Results: Human bone marrow derived MSC were chondrogenically differentiated in 3D culture...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836730/the-effect-of-using-bone-marrow-mesenchymal-stem-cells-versus-platelet-rich-plasma-on-the-healing-of-induced-oral-ulcer-in-albino-rats
#15
Fatma Mohamed Rashed, Olfat Mohamed GabAllah, Sarah Yasser AbuAli, Mohamed Taha Shredah
Background and Objectives: Oral ulceration is one of the most common debilitating condition that affects the oral cavity. In this study, the effect of locally injected platelet rich plasma (PRP) and bone marrow-derived mesenchymal stem cells (BMSCs) on the healing of oral ulcer was investigated. Methods and Results: An ulcer was induced in buccal mucosa of rats by using 5mm biopsy punch followed by application of cotton swab soaked with formocresol for 60sec. The ulcer was left untreated in the control group, treated with intralesional injection of PRP, or isolated cultured BMSCs...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836729/generation-of-organotypic-multicellular-spheres-by-magnetic-levitation-model-for-the-study-of-human-hematopoietic-stem-cells-microenvironment
#16
Claudia Camila Mejía-Cruz, Emilia Barreto-Durán, María Alejandra Pardo-Pérez, María Camila Jimenez, Julieth Rincón, Karen Vanegas, Jorge Luis Rodríguez, Luis Fernando Jaramillo-Garcia, Juan Carlos Ulloa, Rodolfo Martínez Díaz, Efrain Leal-García, Rafael Pérez-Núñez, Alfonso Barreto, Viviana M Rodríguez-Pardo
Background and Objective: The characteristics of human hematopoietic stem cells are conditioned by the microenvironment of the bone marrow, where they interact with other cell populations, such as mesenchymal stem cells and endothelial cells; however, the study of this microenvironment is complex. The objective of this work was to develop a 3D culture system by magnetic levitation that imitates the microenvironment of human HSC. Methods and Results: Human bone marrow-mesenchymal stem cells, umbilical cord blood-hematopoietic stem cells and a non-tumoral endothelial cell line (CC2811, LonzaⓇ ) were used to develop organotypic multicellular spheres by the magnetic levitation method...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836728/incidence-risk-factors-and-prognosis-of-acute-kidney-injury-following-hematopoietic-stem-cell-transplant-a-pilot-study
#17
Amani Anwar Khalil, Laiali T Khalil, Abdalla Awidi
Background: The burden of acute kidney injury (AKI) has not been explored in Jordanian patients who receive hematopoietic stem cell transplant (HSCT). Objective: The aim of this study was to evaluate the frequency, risk factors, and mortality of AKI among patients who underwent HSCT. Methods: A retrospective pilot study included 70 adult patients who received peripheral HSCT was conducted. Weekly measurement of serum creatinine (SCr) was obtained for 3 months after chemotherapy and HSCT...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836727/splitomicin-a-sirt1-inhibitor-enhances-hematopoietic-differentiation-of-mouse-embryonic-stem-cells
#18
Jeong-A Park, Sangkyu Park, Woo-Youn Park, Myung-Kwan Han, Younghee Lee
Background and Objectives: Embryonic stem (ES) cells have pluripotent ability to differentiate into multiple tissue lineages. SIRT1 is a class III histone deacetylase which modulates chromatin remodeling, gene silencing, cell survival, metabolism, and development. In this study, we examined the effects of SIRT1 inhibitors on the hematopoietic differentiation of mouse ES cells. Methods and Results: Treatment with the SIRT1 inhibitors, nicotinamide and splitomicin, during the hematopoietic differentiation of ES cells enhanced the production of hematopoietic progenitors and slightly up-regulated erythroid and myeloid specific gene expression...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836726/monitoring-glutathione-dynamics-and-heterogeneity-in-living-stem-cells
#19
Eui Man Jeong, Ji-Woong Shin, Jisun Lim, Ju Hwan Kim, Hyewon Kang, Yingfu Yin, Hye-Mi Kim, YongHwan Kim, Sun-Gi Kim, Heun-Soo Kang, Dong-Myung Shin, Kihang Choi, In-Gyu Kim
Glutathione (GSH) is a major antioxidant in cells, and plays vital roles in the cellular defense against oxidants and in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow cytometry...
February 28, 2019: International Journal of Stem Cells
https://read.qxmd.com/read/30836725/crispr-cas9-edited-srage-mscs-protect-neuronal-death-in-parkinson-s-disease-model
#20
Jaesuk Lee, Delger Bayarsaikhan, Roshini Arivazhagan, Hyejung Park, Byungyoon Lim, Peter Gwak, Goo-Bo Jeong, Jaewon Lee, Kyunghee Byun, Bonghee Lee
Background and Objectives: Parkinson's disease (PD) is a fatal and progressive degenerative disease of the nervous system. Until recently, its promising treatment and underlying mechanisms for neuronal death are poorly understood. This study was investigated to identify the molecular mechanism of neuronal death in the substantia nigra and corpus striatum of PD. Methods: The soluble RAGE (sRAGE) secreting Umbilical Cord Blood-derived Mesenchymal Stem Cell (UCB-MSC) was generated by gene editing method using clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9)...
February 28, 2019: International Journal of Stem Cells
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