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JOURNAL ARTICLE
Hannah K Jackson, Heather M Long, Juan Carlos Yam-Puc, Roberta Palmulli, Tracey A Haigh, Pehuén Pereyra Gerber, Jin S Lee, Nicholas J Matheson, Lesley Young, John Trowsdale, Mathew Lo, Graham S Taylor, James E Thaventhiran, James R Edgar
The COVID-19 pandemic highlighted the clear risk that zoonotic viruses pose to global health and economies. The scientific community responded by developing several efficacious vaccines which were expedited by the global need for vaccines. The emergence of SARS-CoV-2 breakthrough infections highlights the need for additional vaccine modalities to provide stronger, long-lived protective immunity. Here we report the design and preclinical testing of small extracellular vesicles (sEVs) as a multi-subunit vaccine...
February 2024: Journal of Extracellular Vesicles
#22
JOURNAL ARTICLE
Joshua A Welsh, Deborah C I Goberdhan, Lorraine O'Driscoll, Edit I Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A P Driedonks, Uta Erdbrügger, Juan M Falcon-Perez, Qing-Ling Fu, Andrew F Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M Bridges, Marco Brucale, Dylan Burger, Randy P Carney, Emanuele Cocucci, Federico Colombo, Rossella Crescitelli, Edveena Hanser, Adrian L Harris, Norman J Haughey, An Hendrix, Alexander R Ivanov, Tijana Jovanovic-Talisman, Nicole A Kruh-Garcia, Vroniqa Ku'ulei-Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M Lennon, Jan Lötvall, Adam L Maddox, Elena S Martens-Uzunova, Rachel R Mizenko, Lauren A Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S Sandau, Julie A Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter-Ovanesyan, Juan P Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J C van Herwijnen, Marca H M Wauben, Ann M Wehman, Sarah Williams, Andrea Zendrini, Alan J Zimmerman, Misev Consortium, Clotilde Théry, Kenneth W Witwer
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies...
February 2024: Journal of Extracellular Vesicles
#23
JOURNAL ARTICLE
Zhengsheng Chen, Lei Luo, Teng Ye, Jiacheng Zhou, Xin Niu, Ji Yuan, Ting Yuan, Dehao Fu, Haiyan Li, Qing Li, Yang Wang
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinical translational potential in multiple aging-related degenerative diseases. Characterizing the PSC-sEVs is crucial for their clinical applications. However, the specific marker pattern of PSC-sEVs remains unknown. Here, the sEVs derived from two typical types of PSCs including induced pluripotent stem cells (iPSC-sEVs) and embryonic stem cells (ESC-sEVs) were analysed using proteomic analysis by liquid chromatography with tandem mass spectrometry (LC-MS/MS), and surface marker phenotyping analysis by nanoparticle flow cytometry (NanoFCM)...
February 2024: Journal of Extracellular Vesicles
#24
JOURNAL ARTICLE
Yuhua Gao, Ningning Mi, Wenxiang Wu, Yuxuan Zhao, Fangzhou Fan, Wangwei Liao, Yongliang Ming, Weijun Guan, Chunyu Bai
Extracellular vesicles (EVs) exert a significant influence not only on the pathogenesis of diseases but also on their therapeutic interventions, contingent upon the variances observed in their originating cells. Mitochondria can be transported between cells via EVs to promote pathological changes. In this study, we found that EVs derived from M1 macrophages (M1-EVs), which encapsulate inflammatory mitochondria, can penetrate pancreatic beta cells. Inflammatory mitochondria fuse with the mitochondria of pancreatic beta cells, resulting in lipid peroxidation and mitochondrial disruption...
February 2024: Journal of Extracellular Vesicles
#25
(no author information available yet)
No abstract text is available yet for this article.
February 2024: Journal of Extracellular Vesicles
#26
JOURNAL ARTICLE
Liang Dong, Mingxiao Feng, Morgan D Kuczler, Kengo Horie, Chi-Ju Kim, Zehua Ma, Kara Lombardo, Heather Lyons, Sarah R Amend, Max Kates, Trinity J Bivalacqua, David McConkey, Wei Xue, Woonyoung Choi, Kenneth J Pienta
mRNA-based molecular subtypes have implications for bladder cancer prognosis and clinical benefit from certain therapies. Whether small extracellular vesicles (sEVs) can reflect bladder cancer molecular subtypes is unknown. We performed whole transcriptome RNA sequencing for formalin fixed paraffin embedded (FFPE) tumour tissues and sEVs separated from matched tissue explants, urine and plasma in patients with bladder cancer. sEVs were separated using size-exclusion chromatography, and characterized by transmission electron microscopy, nano flow cytometry and western blots, respectively...
February 2024: Journal of Extracellular Vesicles
#27
(no author information available yet)
No abstract text is available yet for this article.
January 2024: Journal of Extracellular Vesicles
#28
JOURNAL ARTICLE
Ornella Urzì, Markus Bergqvist, Cecilia Lässer, Marta Moschetti, Junko Johansson, Daniele D Arrigo, Roger Olofsson Bagge, Rossella Crescitelli
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be influenced by cell culture conditions such as the presence of foetal bovine serum (FBS). Although several studies have evaluated the effect of removing FBS-derived EVs by ultracentrifugation (UC), less is known about the influence of FBS heat inactivation (HI) on the cell-derived EVs. To assess this, three protocols based on different combinations of EV depletion by UC and HI were evaluated, including FBS ultracentrifuged but not heat inactivated (no-HI FBS), FBS heat inactivated before EV depletion (HI-before EV-depl FBS), and FBS heat inactivated after EV depletion (HI-after EV-depl FBS)...
January 2024: Journal of Extracellular Vesicles
#29
JOURNAL ARTICLE
Hyun Young Kim, Younggap Lim, Ji Sun Jang, Yeon Kyeong Ko, Youngnim Choi, Hong-Hee Kim, Bong-Kyu Choi
Plasminogen activator inhibitor-1 (PAI-1) is associated with nonalcoholic fatty liver disease (NAFLD) by lipid accumulation in the liver. In this study, we showed that extracellular vesicles (EVs) from the periodontal pathogens Filifactor alocis and Porphyromonas gingivalis induced steatosis by inducing PAI-1 in the liver and serum of mice fed a low-fat diet. PAI-1 induction was not observed in TLR2-/- mice. When tested using HEK-Blue hTLR2 cells, human TLR2 reporter cells, the TLR2-activating ability of serum from NAFLD patients (n = 100) was significantly higher than that of serum from healthy subjects (n = 100)...
January 2024: Journal of Extracellular Vesicles
#30
JOURNAL ARTICLE
Rokas Juodeikis, Carlo Martins, Gerhard Saalbach, Jake Richardson, Todor Koev, Dave J Baker, Marianne Defernez, Martin Warren, Simon R Carding
Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation and interspecies and interkingdom communication. However, the factors that regulate their release and heterogeneity are not well understood. We set out to investigate these factors in the common gut commensal Bacteroides thetaiotaomicron by studying BEV release throughout their growth cycle. Utilising a range of methods, we demonstrate that vesicles released at different stages of growth have significantly different composition, with early vesicles enriched in specifically released outer membrane vesicles (OMVs) containing a larger proportion of lipoproteins, while late phase BEVs primarily contain lytic vesicles with enrichment of cytoplasmic proteins...
January 2024: Journal of Extracellular Vesicles
#31
REVIEW
Rienk Nieuwland, Pia R-M Siljander
Blood is the most commonly used body fluid for obtaining and studying extracellular vesicles (EVs). While blood is a standard choice for clinical analysis, using blood as a source of EVs introduces multiple layers of complexity. At the Blood Extracellular Vesicle Workshop organized by the International Society for Extracellular Vesicles in Helsinki (2022), it became evident that beginner researchers lack trustworthy information on how to initiate their research and avoid common pitfalls. This educational guide explains the composition and frequently used terminology of blood, provides guidelines for blood collection, and the preparation of plasma and serum...
January 2024: Journal of Extracellular Vesicles
#32
JOURNAL ARTICLE
Morgan C Pait, Sarah D Kaye, Yixin Su, Ashish Kumar, Sangeeta Singh, Stephen C Gironda, Samantha Vincent, Maria Anwar, Caitlin M Carroll, James Andy Snipes, Jingyun Lee, Cristina M Furdui, Gagan Deep, Shannon L Macauley
Brain-derived extracellular vesicles (EVs) play an active role in Alzheimer's disease (AD), relaying important physiological information about their host tissues. The internal cargo of EVs is protected from degradation, making EVs attractive AD biomarkers. However, it is unclear how circulating EVs relate to EVs isolated from disease-vulnerable brain regions. We developed a novel method for collecting EVs from the hippocampal interstitial fluid (ISF) of live mice. EVs (EVISF ) were isolated via ultracentrifugation and characterized by nanoparticle tracking analysis, immunogold labelling, and flow cytometry...
January 2024: Journal of Extracellular Vesicles
#33
JOURNAL ARTICLE
Nazma F Ilahibaks, Thomas A Kluiver, Olivier G de Jong, Saskia C A de Jager, Raymond M Schiffelers, Pieter Vader, Weng Chuan Peng, Zhiyong Lei, Joost P G Sluijter
The loss-of-function of the proprotein convertase subtilisin-kexin type 9 (Pcsk9) gene has been associated with significant reductions in plasma serum low-density lipoprotein cholesterol (LDL-C) levels. Both CRISPR/Cas9 and CRISPR-based editor-mediated Pcsk9 inactivation have successfully lowered plasma LDL-C and PCSK9 levels in preclinical models. Despite the promising preclinical results, these studies did not report how vehicle-mediated CRISPR delivery inactivating Pcsk9 affected low-density lipoprotein receptor recycling in vitro or ex vivo...
January 2024: Journal of Extracellular Vesicles
#34
JOURNAL ARTICLE
Simonides Immanuel van de Wakker, Julia Bauzá-Martinez, Carla Ríos Arceo, Herak Manjikian, Christian Jamie Bernard Snijders Blok, Marieke Theodora Roefs, Eduard Willms, Renee Goverdina Catharina Maas, Matti Feije Pronker, Olivier Gerrit de Jong, Wei Wu, André Görgens, Samir El Andaloussi, Joost Petrus Gerardus Sluijter, Pieter Vader
Cardiac progenitor cell (CPC)-derived small extracellular vesicles (sEVs) exhibit great potential to stimulate cardiac repair. However, the multifaceted nature of sEV heterogeneity presents a challenge in understanding the distinct mechanisms underlying their regenerative abilities. Here, a dual-step multimodal flowthrough and size-exclusion chromatography method was applied to isolate and separate CPC-derived sEV subpopulations to study the functional differences related to cardiac repair responses. Three distinct sEV subpopulations were identified with unique protein profiles...
January 2024: Journal of Extracellular Vesicles
#35
JOURNAL ARTICLE
Ludovic Giloteaux, Katherine A Glass, Arnaud Germain, Carl J Franconi, Sheng Zhang, Maureen R Hanson
In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue and reduces the risk of chronic diseases. Although exercise is suggested as a lifestyle intervention to manage various chronic illnesses, it negatively affects people with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), who suffer from exercise intolerance. We hypothesized that altered extracellular vesicle (EV) signalling in ME/CFS patients after an exercise challenge may contribute to their prolonged and exacerbated negative response to exertion (post-exertional malaise)...
January 2024: Journal of Extracellular Vesicles
#36
JOURNAL ARTICLE
Ursula S Sandau, Setty M Magaña, Júlia Costa, John P Nolan, Tsuneya Ikezu, Laura J Vella, Hannah K Jackson, Lissette Retana Moreira, Paola Loreto Palacio, Andrew F Hill, Joseph F Quinn, Kendall R Van Keuren-Jensen, Trevor J McFarland, Joanna Palade, Eric A Sribnick, Huaqi Su, Kostas Vekrellis, Beth Coyle, You Yang, Juan M Falcón-Perez, Rienk Nieuwland, Julie A Saugstad
Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS...
January 2024: Journal of Extracellular Vesicles
#37
REVIEW
Sophie Rome, Stefano Tacconi
Recent works indicate that the lipid composition of extracellular vesicles (EVs) can modify their biological functions and their incorporation into recipient cells. In particular high-fat diets affect EV biogenesis, EV lipid composition, EV targeting and consequently the cross-talk between tissues. This review connects different research topics to show that a vicious circle is established during the development of high-fat diet-induced obesity, connecting the alteration of lipid metabolism, the composition of extracellular vesicles and the spread of deleterious lipids between tissues, which participates in NAFLD/NASH and diabetes development...
January 2024: Journal of Extracellular Vesicles
#38
JOURNAL ARTICLE
Hui Gao, Yuxiao Zeng, Xiaona Huang, Luodan A, Qingle Liang, Jing Xie, Xi Lin, Jing Gong, Xiaotang Fan, Ting Zou, Haiwei Xu
Retinal degeneration (RD), a group of diseases leading to irreversible vision loss, is characterised by retinal pigment epithelium (RPE) or retinal neuron damage and loss. With fewer risks of immune rejection and tumorigenesis, stem cell-secreted extracellular vesicles (EVs) offer a new cell-free therapeutic paradigm for RD, which remains to be investigated. Human retinal organoid-derived retinal progenitor cells (hERO-RPCs) are an easily accessible and advanced cell source for RD treatment. However, hERO-RPCs-derived EVs require further characterisation...
January 2024: Journal of Extracellular Vesicles
#39
JOURNAL ARTICLE
Laura Dietz, Jennifer Oberländer, Ana Mateos-Maroto, Jenny Schunke, Michael Fichter, Eva-Maria Krämer-Albers, Katharina Landfester, Volker Mailänder
The influence of a protein corona on the uptake of nanoparticles in cells has been demonstrated in various publications over the last years. Extracellular vesicles (EVs), can be seen as natural nanoparticles. However, EVs are produced under different cell culture conditions and little is known about the protein corona forming on EVs and its influence on their uptake by target cells. Here, we use a proteomic approach in order to analyze the protein composition of the EVs themselves and the protein composition of a human blood plasma protein corona around EVs...
December 2023: Journal of Extracellular Vesicles
#40
JOURNAL ARTICLE
Sam Vanherle, Jeroen Guns, Melanie Loix, Fleur Mingneau, Tess Dierckx, Flore Wouters, Koen Kuipers, Tim Vangansewinkel, Esther Wolfs, Paula Pincela Lins, Annelies Bronckaers, Ivo Lambrichts, Jonas Dehairs, Johannes V Swinnen, Sanne G S Verberk, Mansour Haidar, Jerome J A Hendriks, Jeroen F J Bogie
Macrophages play major roles in the pathophysiology of various neurological disorders, being involved in seemingly opposing processes such as lesion progression and resolution. Yet, the molecular mechanisms that drive their harmful and benign effector functions remain poorly understood. Here, we demonstrate that extracellular vesicles (EVs) secreted by repair-associated macrophages (RAMs) enhance remyelination ex vivo and in vivo by promoting the differentiation of oligodendrocyte precursor cells (OPCs). Guided by lipidomic analysis and applying cholesterol depletion and enrichment strategies, we find that EVs released by RAMs show markedly elevated cholesterol levels and that cholesterol abundance controls their reparative impact on OPC maturation and remyelination...
December 2023: Journal of Extracellular Vesicles
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