journal
https://read.qxmd.com/read/38668303/seroprevalence-and-risk-factors-associated-with-phleboviruses-and-crimean-congo-hemorrhagic-fever-virus-among-blood-donors-in-central-tunisia
#1
JOURNAL ARTICLE
Rym Ayari, Houda Chaouch, Stephen Findlay-Wilson, Wissem Hachfi, Nadia Ben Lasfar, Foued Bellazreg, Stuart Dowall, Neila Hannachi, Amel Letaief
The aim of this study was to determine the prevalence of six viruses, from two families of the order Bunyavirales , in the general population of central Tunisia. Sera collected from 377 asymptomatic blood donors were serologically assayed for Rift Valley fever virus (RVFV), Crimean-Congo hemorrhagic fever virus (CCHFV), and four sandfly-borne phleboviruses: Toscana virus (TOSV), sandfly fever Naples virus (SFNV), sandfly fever Sicilian virus (SFSV), and sandfly fever Cyprus virus (SFCV). Of the 377 subjects enrolled in this study, 17...
April 22, 2024: Pathogens
https://read.qxmd.com/read/38668302/spatial-distribution-of-dermanyssus-gallinae-infestations-in-greece-and-their-association-with-ambient-temperature-humidity-and-altitude
#2
JOURNAL ARTICLE
Georgios Sioutas, Athanasios I Gelasakis, Elias Papadopoulos
Dermanyssus gallinae , the poultry red mite (PRM), is the most prevalent and harmful ectoparasite of laying hens globally. Although prevalence and risk factor studies can help veterinarians make decisions regarding farm treatments, relevant data are scarce. The present study investigated the prevalence and infestation severity of PRM in poultry farms across Greece and examined potential risk factors. AviVet traps were used to sample 84 farms (51 backyard, 33 industrial) over three years. Farm altitude, temperature, humidity, region, and production systems were assessed as potential risk factors with chi-square tests, initially for all the studied farms and then exclusively for backyard farms...
April 22, 2024: Pathogens
https://read.qxmd.com/read/38668301/exploring-extended-spectrum-beta-lactamase-esbl-producing-escherichia-coli-in-food-producing-animals-and-animal-derived-foods
#3
REVIEW
Laryssa Freitas Ribeiro, Natália Maramarque Nespolo, Gabriel Augusto Marques Rossi, John Morris Fairbrother
Antimicrobials serve as crucial treatments in both veterinary and human medicine, aiding in the control and prevention of infectious diseases. However, their misuse or overuse has led to the emergence of antimicrobial resistance, posing a significant threat to public health. This review focuses on extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in animals and their associated food products, which contribute to the proliferation of antimicrobial-resistant strains. Recent research has highlighted the presence of ESBL-producing E...
April 22, 2024: Pathogens
https://read.qxmd.com/read/38668300/molecular-detection-of-hpv-ebv-hsv-1-hcmv-and-h-pylori-pathogens-an-evaluation-among-polish-children-with-molar-incisor-hypomineralization-mih
#4
JOURNAL ARTICLE
Wojciech Tynior, Agata Świętek, Dorota Hudy, Danuta Ilczuk-Rypuła, Joanna Katarzyna Strzelczyk
Molar incisor hypomineralization (MIH) is a congenital disorder of the enamel tissue, characterized by a quantitative deficiency. In childhood, infections such as EBV, HSV-1, HCMV, or H. pylori may occur and cause various diseases. This study aimed to investigate the prevalence of HPV, EBV, HSV-1, HCMV, and H. pylori infections in two groups of children: children with molar incisor hypomineralization (MIH) and a control group, using molecular methods. The study group included 47 children aged between 6-13 years who had been diagnosed with MIH...
April 22, 2024: Pathogens
https://read.qxmd.com/read/38668299/trends-in-non-tuberculous-mycobacterial-lung-disease-and-treatment-outcomes-in-a-low-tuberculosis-prevalence-setting-a-retrospective-analysis
#5
JOURNAL ARTICLE
Biplob Kumar Mohanty, Tomas Mikal Lind Eagan, Bernt Bøgvald Aarli, Dag Harald Skutlaberg, Tehmina Mustafa
BACKGROUND: Information on the management of non-tuberculous mycobacterial (NTM) lung infection and disease is scarce. The aim of this study was to investigate the trends in NTM lung infections, and the factors associated with the initiation of treatment and treatment outcomes. METHODS: A retrospective analysis was carried out on patient medical records from Haukeland University Hospital, Bergen, Norway, from 2000 to 2021. RESULTS: Among 154 patients with NTM lung infection, the majority (70%) were older than 65 years, and 49% had an underlying pulmonary comorbidity...
April 22, 2024: Pathogens
https://read.qxmd.com/read/38668298/-staphylococcus-hsinchuensis-sp-nov-isolated-from-soymilk
#6
JOURNAL ARTICLE
Yu-Ting Wang, Yu-Chun Lin, Yi-Huei Hsieh, Yu-Tzu Lin, Moriyuki Hamada, Chih-Chieh Chen, Jong-Shian Liou, Ai-Yun Lee, Wei-Ling Zhang, Yung-Tsung Chen, Chien-Hsun Huang
A novel coagulase-negative Staphylococcus strain (H164T ) was isolated from soymilk in Taiwan. Comparative sequence analysis of the 16S rRNA gene revealed that the H164T strain is a member of the genus Staphylococcus . We used multilocus sequence analysis (MLSA) and phylogenomic analyses to demonstrate that the novel strain was closely related to Staphylococcus gallinarum , Staphylococcus nepalensis , Staphylococcus cohnii , and Staphylococcus urealyuticus . The average nucleotide identity and digital DNA-DNA hybridization values between H164T and its closest relatives were <95% and <70%, respectively...
April 21, 2024: Pathogens
https://read.qxmd.com/read/38668297/antimicrobial-activity-of-methylene-blue-associated-with-photodynamic-therapy-in-vitro-study-in-multi-species-oral-biofilm
#7
JOURNAL ARTICLE
Bruno Bueno-Silva, Javier Parma-Garcia, Lucio Frigo, Lina J Suárez, Tatiane Tiemi Macedo, Fábio Hideaki Uyeda, Marcelo Augusto Ruiz da Cunha Melo, Roberto Sacco, Carlos Fernando Mourão, Magda Feres, Jamil Awad Shibli, Luciene Cristina Figueiredo
The control of infectious diseases caused by biofilms is a continuing challenge for researchers due to the complexity of their microbial structures and therapeutic implications. Photodynamic therapy as an adjunctive anti-infective treatment has been described as a possible valid approach but has not been tested in polymicrobial biofilm models. This study evaluated the effect of photodynamic therapy in vitro with methylene blue (MB) 0.01% and red LEDs (λ = 660 nm, power density ≈ 330 mW/cm2 , 2 mm distance from culture) on the metabolic activity and composition of a multispecies subgingival biofilm...
April 21, 2024: Pathogens
https://read.qxmd.com/read/38668296/simultaneous-detection-of-porcine-respiratory-coronavirus-porcine-reproductive-and-respiratory-syndrome-virus-swine-influenza-virus-and-pseudorabies-virus-via-quadruplex-one-step-rt-qpcr
#8
JOURNAL ARTICLE
Yan Ma, Kaichuang Shi, Zhenhai Chen, Yuwen Shi, Qingan Zhou, Shenglan Mo, Haina Wei, Liping Hu, Meilan Mo
Porcine respiratory coronavirus (PRCoV), porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), and pseudorabies virus (PRV) are significant viruses causing respiratory diseases in pigs. Sick pigs exhibit similar clinical symptoms such as fever, cough, runny nose, and dyspnea, making it very difficult to accurately differentially diagnose these diseases on site. In this study, a quadruplex one-step reverse-transcription real-time quantitative PCR (RT-qPCR) for the detection of PRCoV, PRRSV, SIV, and PRV was established...
April 19, 2024: Pathogens
https://read.qxmd.com/read/38668295/descriptive-epidemiology-of-pathogens-associated-with-acute-respiratory-infection-in-a-community-based-study-of-k-12-school-children-2015-2023
#9
JOURNAL ARTICLE
Cristalyne Bell, Maureen Goss, Derek Norton, Shari Barlow, Emily Temte, Cecilia He, Caroline Hamer, Sarah Walters, Alea Sabry, Kelly Johnson, Guanhua Chen, Amra Uzicanin, Jonathan Temte
School-based outbreaks often precede increased incidence of acute respiratory infections in the greater community. We conducted acute respiratory infection surveillance among children to elucidate commonly detected pathogens in school settings and their unique characteristics and epidemiological patterns. The ORegon CHild Absenteeism due to Respiratory Disease Study (ORCHARDS) is a longitudinal, laboratory-supported, school-based, acute respiratory illness (ARI) surveillance study designed to evaluate the utility of cause-specific student absenteeism monitoring for early detection of increased activity of influenza and other respiratory viruses in schools from kindergarten through 12th grade...
April 19, 2024: Pathogens
https://read.qxmd.com/read/38668294/chronic-hepatitis-c-virus-infection-extrahepatic-disease-and-the-impact-of-new-direct-acting-antivirals
#10
REVIEW
Nahum Méndez-Sánchez, Carlos E Coronel-Castillo, Mariana Michelle Ramírez-Mejía
Chronic hepatitis C virus infection is an important cause of liver cirrhosis, hepatocellular carcinoma and death. Furthermore, it is estimated that about 40-70% of patients develop non-hepatic alterations in the course of chronic infection. Such manifestations can be immune-related conditions, lymphoproliferative disorders and metabolic alterations with serious adverse events in the short and long term. The introduction of new Direct-Acting Antivirals has shown promising results, with current evidence indicating an improvement and remission of these conditions after a sustained virological response...
April 19, 2024: Pathogens
https://read.qxmd.com/read/38668293/higher-prevalence-of-the-periodontal-pathogen-selenomonas-noxia-among-pediatric-and-adult-patients-may-be-associated-with-overweight-and-obesity
#11
JOURNAL ARTICLE
Austin Williams, Jace Porter, Karl Kingsley, Katherine M Howard
New evidence has suggested that oral and gut microflora may have significant impacts on the predisposition, development, and stability of obesity in adults over time-although less is known about this phenomenon in children. Compared with healthy-weight controls, overweight and obese adult patients are now known to harbor specific pathogens, such as Selenomonas noxia ( S. noxia ), that are capable of digesting normally non-digestible cellulose and fibers that significantly increase caloric extraction from normal dietary intake...
April 19, 2024: Pathogens
https://read.qxmd.com/read/38668292/-isospora-and-lankesterella-parasites-eimeriidae-apicomplexa-of-passeriform-birds-in-europe-infection-rates-phylogeny-and-pathogenicity
#12
JOURNAL ARTICLE
Carina Keckeisen, Alžbeta Šujanová, Tanja Himmel, Julia Matt, Nora Nedorost, Carolina R F Chagas, Herbert Weissenböck, Josef Harl
Wild birds are common hosts to numerous intracellular parasites such as single-celled eukaryotes of the family Eimeriidae (order Eucoccidiorida, phylum Apicomplexa). We investigated the infection rates, phylogeny, and pathogenicity of Isospora and Lankesterella parasites in wild and captive passerine birds. Blood and tissue samples of 815 wild and 15 deceased captive birds from Europe were tested using polymerase chain reaction and partial sequencing of the mitochondrial cytochrome b and cytochrome c oxidase I and the nuclear 18S rRNA gene...
April 18, 2024: Pathogens
https://read.qxmd.com/read/38668291/pradofloxacin-for-treatment-of-bartonella-henselae-in-experimentally-inoculated-cats
#13
JOURNAL ARTICLE
Michael R Lappin, Ronan Fitzgerald
Bartonella henselae is associated with numerous clinical syndromes in people. Cats are the definitive hosts for B. henselae , develop high levels of bacteremia, and are associated with human infections, particularly in the presence of Ctenocephalides felis . Several antibiotic protocols used for the treatment of B. henselae infection in cats have failed to clear bacteremia. The purpose of this study was to assess the safety and efficacy of a high-dose pradofloxacin protocol to eliminate B. henselae bacteremia...
April 18, 2024: Pathogens
https://read.qxmd.com/read/38668290/development-of-a-targeted-ngs-assay-for-the-detection-of-respiratory-pathogens-including-sars-cov-2-in-felines
#14
JOURNAL ARTICLE
Jobin J Kattoor, Mothomang Mlalazi-Oyinloye, Sarah M Nemser, Rebecca P Wilkes
Acute respiratory diseases in felines can be attributed to a diverse range of pathogens. The recent emergence of novel viruses, particularly SARS-CoV-2 and its variants, has also been associated with respiratory ailments in cats and other pets, underscoring the need for a highly sensitive diagnostic assay capable of concurrently detecting multiple respiratory pathogens. In this study, we developed a targeted next generation sequencing panel using Ion Torrent Ampliseq technology to detect multiple respiratory pathogens, including recent SARS-CoV-2 variants and Feline herpesvirus-1, Feline calicivirus, Bordetella bronchiseptica , Mycoplasmopsis (previously Mycoplasma ) felis , and Chlamydia felis ...
April 17, 2024: Pathogens
https://read.qxmd.com/read/38668289/phylodynamic-and-evolution-of-the-hemagglutinin-ha-and-neuraminidase-na-genes-of-influenza-a-h1n1-pdm09-viruses-circulating-in-the-2009-and-2023-seasons-in-italy
#15
JOURNAL ARTICLE
Fabio Scarpa, Leonardo Sernicola, Stefania Farcomeni, Alessandra Ciccozzi, Daria Sanna, Marco Casu, Marco Vitale, Alessia Cicenia, Marta Giovanetti, Chiara Romano, Francesco Branda, Massimo Ciccozzi, Alessandra Borsetti
The influenza A(H1N1) pdm09 virus, which emerged in 2009, has been circulating seasonally since then. In this study, we conducted a comprehensive genome-based investigation to gain a detailed understanding of the genetic and evolutionary characteristics of the hemagglutinin (HA) and neuraminidase (NA) surface proteins of A/H1N1pdm09 strains circulating in Italy over a fourteen-year period from 2009 to 2023 in relation to global strains. Phylogenetic analysis revealed rapid transmission and diversification of viral variants during the early pandemic that clustered in clade 6B...
April 17, 2024: Pathogens
https://read.qxmd.com/read/38668288/molecular-characterization-of-non-h5-and-non-h7-avian-influenza-viruses-from-non-mallard-migratory-waterbirds-of-the-north-american-flyways-2006-2011
#16
JOURNAL ARTICLE
Shahan Azeem, John Baroch, Deepanker Tewari, Kristy L Pabilonia, Mary Killian, Birgit Bradel-Tretheway, Dong Sun, Sara Ghorbani-Nezami, Kyoung-Jin Yoon
The surveillance of migratory waterbirds (MWs) for avian influenza virus (AIV) is indispensable for the early detection of a potential AIV incursion into poultry. Surveying AIV infections and virus subtypes in understudied MW species could elucidate their role in AIV ecology. Oropharyngeal-cloacal (OPC) swabs were collected from non-mallard MWs between 2006 and 2011. OPC swabs ( n = 1158) that molecularly tested positive for AIV (Cts ≤ 32) but tested negative for H5 and H7 subtypes were selected for virus isolation (VI)...
April 17, 2024: Pathogens
https://read.qxmd.com/read/38668287/dengue-associated-hemophagocytic-lymphohistiocytosis-a-narrative-review-of-its-identification-and-treatment
#17
REVIEW
Kay Choong See
Dengue's lack of specific treatments beyond supportive care prompts a focus on uncovering additional pathophysiological factors. Dengue-associated hemophagocytic lymphohistiocytosis (HLH), characterized by dysregulated macrophage activation and cytokine storm, remains underexplored despite its potential to worsen disease severity and mortality. While rare, dengue-associated HLH disproportionately affects severe cases, significantly impacting mortality rates. To mitigate high mortality, early identification and familiarity with dengue-associated HLH are imperative for prompt treatment by clinicians...
April 17, 2024: Pathogens
https://read.qxmd.com/read/38668286/biomarkers-in-detection-of-hepatitis-c-virus-infection
#18
REVIEW
Jungreem Woo, Youkyung Choi
The hepatitis C virus (HCV) infection affects 58 million people worldwide. In the United States, the incidence rate of acute hepatitis C has doubled since 2014; during 2021, this increased to 5% from 2020. Acute hepatitis C is defined by any symptom of acute viral hepatitis plus either jaundice or elevated serum alanine aminotransferase (ALT) activity with the detection of HCV RNA, the anti-HCV antibody, or hepatitis C virus antigen(s). However, most patients with acute infection are asymptomatic. In addition, ALT activity and HCV RNA levels can fluctuate, and a delayed detection of the anti-HCV antibody can occur among some immunocompromised persons with HCV infection...
April 17, 2024: Pathogens
https://read.qxmd.com/read/38668285/minion-sequencing-of-fungi-in-sub-saharan-african-air-and-a-novel-lamp-assay-for-rapid-detection-of-the-tropical-phytopathogenic-genus-lasiodiplodia
#19
JOURNAL ARTICLE
Kevin M King, Gail G M Canning, Jonathan S West
To date, there have been no DNA-based metabarcoding studies into airborne fungi in tropical Sub-Saharan Africa. In this initial study, 10 air samples were collected onto Vaseline-coated acrylic rods mounted on drones flown at heights of 15-50 meters above ground for 10-15 min at three sites in Ghana. Purified DNA was extracted from air samples, the internal transcribed spacer (ITS) region was amplified using fungal-specific primers, and MinION third-generation amplicon sequencing was undertaken with downstream bioinformatics analyses utilizing GAIA cloud-based software (at genus taxonomic level)...
April 17, 2024: Pathogens
https://read.qxmd.com/read/38668284/a-set-of-multiresistant-isolates-of-mycoplasma-bovis-subtype-st-1-with-a-variable-susceptibility-to-quinolones-are-also-circulating-in-spain
#20
JOURNAL ARTICLE
Juan Carlos Corrales, Antonio Sánchez, Xóchitl Hernández, Joaquín Amores-Iniesta, Antón Esnal, Christian de la Fe
Mycoplasma bovis ( M. bovis ) is one of the worldwide most important infectious agents involved in respiratory complex diseases (RCD). In Spain, the endemic presence of subtypes ST-2 and ST-3 with phenotypic differences linked to their susceptibility to fluoroquinolones opened the way to develop control strategies focused on previous diagnosis of the subtype and the use of directed therapies when M. bovis were involved in RCD. Surprisingly, microbiological studies conducted during 2023 evidenced for the first time the presence of Spanish isolates of a new polC -subtype, previously classified as ST-1, recovered from calves with respiratory symptoms and pneumonia in different areas of the country ( n = 16)...
April 16, 2024: Pathogens
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