Sarah Förster, Yeeting E Chong, David Siefker, Yvonne Becker, Ruizhi Bao, Erik Escobedo, Yang Qing, Kaitlyn Rauch, Luke Burman, Christoph Burkart, Philipp Kainz, Andrea Cubitt, Michael Muders, Leslie A Nangle
Neuropilin-2 (NRP2) is a cell surface receptor that plays key roles in lymphangiogenesis, but also in pathophysiological conditions such as cancer and inflammation. NRP2 targeting by efzofitimod, a novel immunomodulatory molecule, is currently being tested for the treatment of pulmonary sarcoidosis. To date, no anti-NRP2 antibodies are available for companion diagnostics. Here we describe the development and characterization of a novel NRP2 antibody. Using a variety of research techniques, that is, enzyme-linked immunoassay, Western blot, biolayer interferometry, and immunohistochemistry, we demonstrate that our antibody detects all major NRP2 isoforms and does not cross-react with NRP1...
October 2023: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy