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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://read.qxmd.com/read/30879262/molecular-detection-and-phylogeny-of-bovine-viral-diarrhea-virus-1-among-cattle-herds-from-northeast-southeast-and-midwest-regions-brazil
#1
Poliana de Oliveira Figueiredo, Danilo Bretas de Oliveira, Leandra Barcelos Figueiredo, Galileu Barbosa Costa, Pedro Augusto Alves, Maria Isabel Maldonado Coelho Guedes, Edel Figueiredo Barbosa-Stancioli, Betânia Paiva Drumond, Jônatas Santos Abrahão, Erna Geessien Kroon, Giliane de Souza Trindade
We examined the circulating BVDV species and genotypes among cattle herds from Northeast, Southeast, and Midwest regions in Brazil. A total of 77 animals tested positive through standard PCR. Phylogenetic analyses revealed the presence of BVDV-1a, highlighting the need for better surveillance strategies to prevent BVDV spread in the country.
March 16, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30877665/ability-of-endophytic-fungi-associated-with-withania-somnifera-l-to-control-fusarium-crown-and-root-rot-and-to-promote-growth-in-tomato
#2
Ahlem Nefzi, Rania Aydi Ben Abdallah, Hayfa Jabnoun-Khiareddine, Nawaim Ammar, Mejda Daami-Remadi
Fusarium crown and root rot (FCRR), caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is a soilborne tomato disease of increased importance worldwide. In this study, Withania somnifera was used as a potential source of biological control and growth-promoting agents. Seven fungal isolates naturally associated with W. somnifera were able to colonize tomato seedlings. They were applied as conidial suspensions or a cell-free culture filtrate. All isolates enhanced treated tomato growth parameters by 21...
March 15, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30877664/genetic-identification-of-pestiviruses-from-beef-cattle-in-southern-brazil
#3
Francielle Liz Monteiro, Bruno Martins, Juliana F Cargnelutti, Jessica G Noll, Rudi Weiblen, Eduardo F Flores
Bovine pestiviruses, e.g., bovine viral diarrhea virus types 1 (BVDV-1 or Pestivirus A), BVDV-2 (Pestivirus B), and HoBi-like pestiviruses (HoBiPeV or Pestivirus H), have been shown to circulate in Brazilian cattle in varied proportions. In this study, we identified genetically pestiviruses circulating in beef cattle in Rio Grande do Sul, the southern most Brazilian state. Screening of serum of 15.584 beef calves destined to be export by an antigen capture ELISA and, subsequently, by reverse-transcription polymerase chain reaction (RT-PCR), revealed 135 containing pestivirus RNA...
March 15, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30877663/divergent-coronaviruses-detected-in-wild-birds-in-brazil-including-a-central-park-in-s%C3%A3-o-paulo
#4
Carla M Barbosa, Edison L Durigon, Luciano M Thomazelli, Tatiana Ometto, Roberta Marcatti, Marcello Shiavo Nardi, Daniel M de Aguiar, João Batista Pinho, Maria Virginia Petry, Isaac Simão Neto, Patrícia Serafini, Roberta Costa Rodrigues, Severino Mendes de Azevedo Junior, Luiz Gustavo B Góes, Jansen de Araujo
Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013...
March 15, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30877662/the-perennial-use-of-the-green-fluorescent-protein-marker-in-a-live-vaccinia-virus-ankara-recombinant-platform-shows-no-acute-adverse-effects-in-mice
#5
D S O Daian E Silva, T M G Pinho, M A Rachid, D F Barbosa-Stancioli, F G Da Fonseca
Recombinant virus vectors represent a promising strategy for vaccine research. Among available viral vectors, members of the Poxviridae family-especially the modified Vaccinia virus Ankara (MVA)-stand out as immunogenic and safe vaccine platforms. Because MVA usually does not produce plaques in cell culture, visible selection markers such as the green fluorescent protein (GFP) are frequently incorporated into the constructions in order to facilitate the recognition of recombinants. However, these genetic markers have to be removed before any clinical trial...
March 15, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30874967/correction-to-effects-of-metaperiodate-and-urea-solutions-on-the-serological-diagnosis-of-human-sporotrichosis-using-an-indirect-elisa-test
#6
Letícia Maria Leomil Coelho, Julianne Caravita Grisolia, Maria Lúcia, Míriam Boczar, Eric Batista Ferreira, Denismar Alves Nogueira, Jorge Kleber Chavasco, Zoilo Pires de Camargo, Leila Maria Lopes-Bezerra, Luiz Felipe Leomil Coelho, Luiz Cosme Cotta Malaquias
In the article mentioned above an author's name was misspelled. The correct author name reads as follows: Leila Maria Lopes-Bezerra. We apologize for the inconvenience.
March 14, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30864077/potential-of-oxygen-and-nitrogen-reactive-intermediates-to-disperse-listeria-monocytogenes-from-biofilms
#7
Fernanda Barbosa Dos Reis-Teixeira, Natália Conceição, Lilian Pereira da Silva, Virgínia Farias Alves, Elaine Cristina Pereira De Martinis
Studying biofilm dispersal is important to prevent Listeria monocytogenes persistence in food processing plants and to avoid finished product contamination. Reactive oxygen and nitrogen intermediates (ROI and RNI, respectively) may trigger cell detachment from many bacterial species biofilms, but their roles in L. monocytogenes biofilms have not been fully investigated. This study reports on ROI and RNI quantification in Listeria monocytogenes biofilms formed on stainless steel and glass surfaces; bacterial culture and microscopy combined with fluorescent staining were employed...
March 13, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30859495/detection-of-enterobacterales-resistant-to-polymyxins-using-rapid-polymyxins-np-test
#8
Tanise Vendruscolo Dalmolin, Graziela Ávila Dias, Luiza Peres de Castro, Helena Ávila, Cibele Massotti Magagnin, Alexandre Prehn Zavascki, Daiana de Lima-Morales, Afonso Luís Barth
Two hundred isolates of Enterobacterales were tested by Rapid Polymyxins NP for the detection of polymyxin resistance and compared to the reference test broth microdilution (BMD). The sensitivity and specificity of the NP test were 98% and the results are faster than the BMD, decreasing from approximately 24 to 2 h.
March 11, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30852798/physiological-and-molecular-insights-of-bacteriocin-production-by-enterococcus-hirae-st57acc-from-brazilian-artisanal-cheese
#9
Valéria Quintana Cavicchioli, Svetoslav Dimitrov Todorov, Ilia Iliev, Iskra Ivanova, Djamel Drider, Luís Augusto Nero
The bacteriocinogenic Enterococcus hirae ST57ACC recently isolated from a Brazilian artisanal cheese was subjected here to additional analyses in order to evaluate its bacteriocin production and the potential influence of ABC transporter system in its expression. Besides these physiological and molecular aspects, the bacteriocin was evaluated for its cytotoxicity against HT-29. Differences in the inoculum size had no impact on the growth of E. hirae ST57ACC; however, the bacteriocin was only produced after 9 h of growth when the strain was inoculated at 5% or 10% (v/v), with similar levels of bacteriocin production obtained by both conventional growth and batch fermentation...
March 9, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30852797/cryptococcus-gattii-vgii-isolated-from-native-forest-and-river-in-northern-brazil
#10
Amaury Dos Santos Bentes, Bodo Wanke, Márcia Dos Santos Lazéra, Ana Karla Lima Freire, Roberto Moreira da Silva Júnior, Diego Fernando Silva Rocha, Silviane Bezerra Pinheiro, Steven Edward Zelski, Ani Beatriz Jackisch Matsuura, Liliane Coelho da Rocha, Erica Simplício de Souza, João Vicente Braga de Souza
BACKGROUND: Cryptococcosis is a global invasive mycosis associated with significant morbidity and mortality. In the northern region of Brazil, this disease is caused by Cryptococcus neoformans genotype VNI and Cryptococcus gattii genotype VGII. However, few environmental studies have been conducted in this large tropical area. AIMS: This study was performed to isolate, genotype, and determine the frequency of cryptococcal agents in environmental samples near Manaus, Amazonas, Brazil...
March 9, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30850979/genome-sequence-of-xanthomonas-fuscans-subsp-fuscans-strain-xff49-a-new-isolate-obtained-from-common-beans-in-southern-brazil
#11
Frederico Schmitt Kremer, Ismail Teodoro de Souza Junior, Amanda Munari Guimarães, Rafael Dos Santos Danelon Woloski, Andrea Bittencourt Moura, Luciano da Silva Pinto
The genus Xanthomonas comprises Gram-negative bacteria, many of which are phytopathogens. Xanthomonas fuscans subsp. fuscans is one of the most devastating pathogens affecting the bean plant, resulting in the common bacterial blight of bean (CBB). The disease is mainly foliar and affects a wide variety of bean species, thus acting as the yield-limiting factor for the bean crop. Here, we report the whole-genome sequencing of a new strain of X. fuscans subsp. fuscans, named Xff49, isolated from the infected and symptomatic beans from Capão do Leão, Southern Brazil...
March 9, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30850978/semisolid-culture-medium-improves-mycelial-recovery-of-agaricus-subrufescens-cryopreserved-in-cereal-grains
#12
Henrique Susumu Tanaka, Miria Benetati Delgado Bertéli, Fabio Aparecido Cordeiro, Ana Daniela Lopes, Juliana Silveira do Valle, Giani Andrea Linde, Nelson Barros Colauto
This study aimed to evaluate the effects of the solid and semisolid culture medium on the mycelial viability of A. subrufescens after 5-year cryopreservation at - 70 °C. Mycelia were grown in three types of whole or ground grains, with or without 5% glycerol addition in the substrate and/or in a cryotube. After 5 years of cryopreservation at - 70 °C, every treatment was thawed and recovered in malt extract culture medium with 15 (solid culture medium) or 5 g L-1 (semisolid culture medium) of agar...
March 8, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30850977/interaction-between-the-production-of-ethanol-and-glycerol-in-fed-batch-bioreactors
#13
Márcia Justino Rossini Mutton, Fernanda C S Ferrari, Lidyane Aline de Freita, Cristhyane Millena de Freita, Maria da Graça S Andrietta, Silvio Roberto Andrietta
During alcoholic fermentation, most of the substrates supplied to the yeasts are converted into ethanol and carbon dioxide generating energy for cell maintenance. However, some of these substrates end up being diverted to other metabolic pathways generating by-products reducing the yield in ethanol production. Glycerol is the most important by-product quantitatively, and its production during fermentation is associated to the production of ethanol. The present study was carried out at a full scale in an industrial fermentation plant applied to sugar cane industry with bioreactors operated in fed-batch mode...
March 8, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30848436/heterologous-expression-and-functional-characterization-of-the-ligand-binding-domain-of-oxysterol-binding-protein-from-aspergillus-oryzae
#14
Long Ma, Xian Zhang, Zhihong Hu, Bin He, Mingqiang Ai, Bin Zeng
Oxysterol-binding proteins (OSBPs) comprise a family of sterol-binding proteins. In this study, we focused on AoOSBP1, one of the five OSBP proteins identified from the industrial fungus Aspergillus oryzae. The temporal expression pattern analysis showed that the expression of AoOSBP1, in both gene and protein levels, was stably expressed throughout the developmental stages, while was upregulated during the accelerated growth stage. The immunofluorescence observation revealed that AoOSBP1 protein was mainly distributed in the conidiophore, indicating its underlying role in spore formation...
March 8, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30835059/outbreak-of-contagious-ecthyma-caused-by-orf-virus-parapoxvirus-ovis-in-a-vaccinated-sheep-flock-in-uruguay
#15
Ricardo Almeida da Costa, Juliana Felipetto Cargnelutti, Carlos Omar Schild, Eduardo Furtado Flores, Franklin Riet-Correa, Federico Giannitti
Orf virus (ORFV) causes contagious ecthyma (CE), a highly transmissible, zoonotic disease of small ruminants. CE most commonly affects lambs and unvaccinated sheep. This work reports epidemiologic, clinicopathologic, and virologic findings in a CE outbreak in a vaccinated sheep flock in Uruguay and failure to detect ORFV in a commercial vaccine.
March 5, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30830602/enterobacter-sp-strain-fs-11-adapted-to-diverse-ecological-conditions-and-promoted-sunflower-achene-yield-nutrient-uptake-and-oil-contents
#16
Muhammad Shahid, Sohail Hameed, Mohsin Zafar, Muhammad Tahir, Muhammad Ijaz, Mohsin Tariq, Khadim Hussain, Amanat Ali
Plant growth-promoting rhizobacteria are under extensive investigation to supplement the chemical fertilizers due to cost-effective and eco-friendly nature. However, their consistency in heterogeneous soil and diverse ecological settings is unclear. The current study presents in vitro and field evaluation of pre-characterized PGPR strain Enterobacter sp. Fs-11 (GenBank accession # GQ179978) in terms of its potential to enhance sunflower yield and oil contents under diverse environmental conditions. Under in vitro conditions, strain Fs-11 showed optimal growth at a range of temperature (15 to 40 °C) and pH values (6...
March 4, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30827000/increase-of-candida-antarctica-lipase-b-production-under-pgk-promoter-in-pichia-pastoris-effect-of-multicopies
#17
Julia Macedo Robert, Maritza Ocampo Betancur, Antonio Carlos Oliveira Machado, Andrelisse Arruda, Viviane Castelo Branco Reis, Rodrigo Volcan Almeida, Fernando Araripe Gonçalves Torres, Pau Ferrer Alegre, Francisco Valero, Denise Maria Guimarães Freire
The effect of gene dosage on the production of Candida antarctica lipase B (CalB) in the methylotrophic yeast Komagataella phaffii, at high densities in a simple medium containing crude glycerin as the sole carbon source, is described. The use of crude glycerin, the main by-product of biodiesel production from vegetable oils, will reduce the production cost of the bioprocess. Two K. phaffii strains were constructed with one or three copies of LipB, an optimized version of the gene encoding CalB under the control of the constitutive PPGK1 promoter...
March 2, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30826999/fermentative-capabilities-of-native-yeast-strains-grown-on-juices-from-different-agave-species-used-for-tequila-and-mezcal-production
#18
M Alcazar-Valle, A Gschaedler, H Gutierrez-Pulido, A Arana-Sanchez, M Arellano-Plaza
The Asparagaceae family is endemic from America, being the Agave genus the most important. The Agave species possess economic relevance and are use as raw material to produce several distilled alcoholic beverages, as bacanora, tequila, and mezcal. The fermentation process has been carry out either spontaneously or by adding a selected yeast strain. The latter is generally responsible for the production of ethanol and volatile compounds. This study comprised five Agave species (A. angustifolia, A. cupreata, A...
March 2, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30806891/fermentation-of-yam-dioscorea-spp-l-by-indigenous-phytase-producing-lactic-acid-bacteria-strains
#19
Nádia Nara Batista, Cíntia Lacerda Ramos, Leonardo de Figueiredo Vilela, Disney Ribeiro Dias, Rosane Freitas Schwan
The use of lactic bacteria in the development of functional foods has increased in recent years. In addition to their probiotic characteristics, they can ferment a variety of substrates, such as cereals, roots, and tubers. Phytase producer lactic acid bacteria strains and their behavior during the fermentation process of yam-based food were studied. Leuconostoc lactis CCMA 0415, Lactobacillus plantarum CCMA 0744, and Lactobacillus fermentum CCMA 0745 were selected due to phytase production, pH reduction, and growth during 24 h of fermentation...
February 26, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/30805894/genetic-polymorphism-in-association-with-susceptibility-to-tuberculosis-a-study-in-a-pakistani-population
#20
AsmaGul Naz, Muhammad Assad Aslam, Abrar Ul Haq Khan, Sumaira Rasul, Hamid Manzoor, Rehana Iqbal, Ahmed Shehzad, Muhammad Ali
Tuberculosis is becoming a global issue with raising occurrences; particularly in developing countries, the situation is alarming. Besides environmental factors, host genetic factors are vital in disease development. A demographical and genotypic analysis in relation to tuberculosis commencement is conducted in a Pakistani population, and genotypic frequency of EBI3 (rs4740) was analyzed. Allelic frequencies of EBI3 (rs4740) were significantly associated with disease susceptibility in the reviewed population...
February 25, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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