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Elina Balodite, Inese Strazdina, Jekaterina Martynova, Nina Galinina, Reinis Rutkis, Zane Lasa, Uldis Kalnenieks
Acetaldehyde, a valuable commodity chemical, is a volatile inhibitory byproduct of aerobic fermentation in Zymomonas mobilis and in several other microorganisms. Attempting to improve acetaldehyde production by minimizing its contact with the cell interior and facilitating its removal from the culture, we engineered a Z. mobilis strain with acetaldehyde synthesis reaction localized in periplasm. For that, the pyruvate decarboxylase (PDC) was transferred from the cell interior to the periplasmic compartment...
February 15, 2019: MicrobiologyOpen
Lin Wang, Haixing Xu, Zewen Liu, Taolei Sun, Chengqing Yuan, Ying Yang, Junhui Guo, Hao Xie
Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3 O4 -SiO2 iron beads and was confirmed via SDS-PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6-HSL...
February 14, 2019: MicrobiologyOpen
Li Huang, Lixing Huang, Lingmin Zhao, Yingxue Qin, Yongquan Su, Qingpi Yan
Vibrio alginolyticus is one of the most important pathogens in mariculture and leading to heavy losses. After treatment with Cu2+ , Pb2+ , and low pH, the expression of oxidative phosphorylation pathway genes, including coxA, coxB, coxC, ccoN, ccoO, and ccoQ, was found commonly downregulated by RNA-seq as well as quantitative real-time PCR. RNAi significantly reduced the expression of coxA, coxB, coxC, ccoN, ccoO, and ccoQ in V. alginolyticus. Compared with the wild-type strain, the adhesion abilities of RNAi strains of V...
February 14, 2019: MicrobiologyOpen
Mousa M Alreshidi, R Hugh Dunstan, Margaret M Macdonald, Nathan D Smith, Johan Gottfries, Tim K Roberts
Staphylococcus aureus is a versatile bacterium that can adapt to survive and grow in a wide range of salt concentrations. This study investigated whether the cells could mount a response to survive a challenge of 5% NaCl in a minimal incubation medium that would not support cell replication. Cells were grown in liquid culture, washed and then incubated for 90 min at 37°C in a medium that contained only glycine and glucose as substrates in PBS plus trace elements. The control cells were compared with a treatment group which was incubated with an additional 5% NaCl...
February 9, 2019: MicrobiologyOpen
Junwei Li, Ruiping Hu, Yongjian Guo, Suwen Chen, Xiaoyong Xie, Jian G Qin, Zhenhua Ma, Changbo Zhu, Surui Pei
To understand the impacts of peanut worms Sipunculus nudus on the prokaryotic community composition in a tidal flat, an onsite investigation was conducted in Suixi in the Beibu Gulf (109.82E, 21.35N) in the burrow sediments, non-burrow sediments and the sediments without peanut worm disturbance (control). The16S rRNA gene Illumina MiSeq sequencing was used to investigate the microbial communities and their response to bioturbation by S. nudus in a sandy tidal flat. A total of 18 bacteria phyla were detected, and Proteobacteria and Cyanobacteria constituted the majority of the prokaryotic community in the samples...
February 7, 2019: MicrobiologyOpen
Yi-Hua Lyu, Yue-Xia Zhou, Yi Li, Jin Zhou, Yi-Xiao Xu
Bacteria play an important role in preventing algal blooms and reducing their harm to the environment. To improve the algicidal activity of Pseudoalteromonas SP48 which had an inhibition effect on dinoflagellate Alexandrium tamarense, its growth medium and fermentation conditions were optimized for this bacterium. In this study, we used two steps to establish the optimum conditions. First, the proper proportion of medium was selected based on an orthogonal design. Then, the fermentation conditions were further optimized through uniform design in an enlarged 5L bioreactor...
February 7, 2019: MicrobiologyOpen
Agnieszka Staniszewska, Alina Kunicka-Styczyńska, Anna Otlewska, Jan Gawor, Robert Gromadka, Karolina Żuchniewicz, Krzysztof Ziemiński
This study provides a deep modern insight into the phylogenetic diversity among bacterial consortia found in working and nonworking high-methane natural gas pipelines located in Poland. The working pipeline was characterized by lower biodiversity (140-154 bacterial genera from 22 to 23 classes, depending on the source of the debris) in comparison to the off-gas pipeline (169 bacterial genera from 23 classes). The sediment recovered from the working pipeline contained mostly DNA identified as belonging to the phylum Firmicutes (66...
February 6, 2019: MicrobiologyOpen
Konrad Kosciow, Uwe Deppenmeier
The gut microbe Akkermansia muciniphila is important for the human health as the occurrence of the organism is inversely correlated with different metabolic disorders. The metabolism of the organism includes the degradation of intestinal mucins. Thus, the gut health-promoting properties are not immediately obvious and mechanisms of bacteria-host interactions are mostly unclear. In this study, we characterized a novel extracellular β-galactosidase (Amuc_1686) with a preference for linkages from the type Galβ1-3GalNAc...
February 6, 2019: MicrobiologyOpen
Sory Ibrahima Traore, Melhem Bilen, Mamadou Beye, Awa Diop, Maxime Descartes Mbogning Fonkou, Mamadou Lamine Tall, Caroline Michelle, Muhammad Yasir, Esam Ibraheem Azhar, Fehmida Bibi, Fadi Bittar, Asif Ahmad Jiman-Fatani, Ziad Daoud, Fréderic Cadoret, Pierre-Edouard Fournier, Sophie Edouard
As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille-P2849T was isolated from the fecal specimen of a healthy 19-year-old Saudi Bedouin, while R. timonensis type strain Marseille-P3277T was isolated from the feces of an 11-year-old pygmy female living in Congo...
January 30, 2019: MicrobiologyOpen
Erika S Varady, Sohrab Bodaghi, Georgios Vidalakis, Greg W Douhan
Penicillium digitatum is one of the most important postharvest pathogens of citrus on a global scale causing significant annual losses due to fruit rot. However, little is known about the diversity of P. digitatum populations. The genome of P. digitatum has been sequenced, providing an opportunity to determine the microsatellite distribution within P. digitatum to develop markers that could be valuable tools for studying the population biology of this pathogen. In the analyses, a total of 3,134 microsatellite loci were detected; 66...
January 30, 2019: MicrobiologyOpen
Chishih Chu, Min Yi Wong, Yuan-Hsi Tseng, Chun-Liang Lin, Chun-Wu Tung, Chih-Chen Kao, Yao-Kuang Huang
Hemodialysis patients are particularly vulnerable to Staphylococcus aureus infection, with the vascular access serving as the site of entry for this formidable pathogen. Patients with arteriovenous grafts (AVGs) and tunneled-cuffed catheters (TCCs) are at elevated risk of S. aureus infection. In this study, we investigated the correlation between the clinical characteristics of S. aureus vascular access infection (VAI), molecular profiles, and the biofilm formation abilities of clinical isolates of S. aureus...
January 25, 2019: MicrobiologyOpen
Sara B Pereira, Marina Santos, José P Leite, Carlos Flores, Carina Eisfeld, Zsófia Büttel, Rita Mota, Federico Rossi, Roberto De Philippis, Luís Gales, João H Morais-Cabral, Paula Tamagnini
Many cyanobacteria produce extracellular polymeric substances (EPS) mainly composed of heteropolysaccharides with unique characteristics that make them suitable for biotechnological applications. However, manipulation/optimization of EPS biosynthesis/characteristics is hindered by a poor understanding of the production pathways and the differences between bacterial species. In this work, genes putatively related to different pathways of cyanobacterial EPS polymerization, assembly, and export were targeted for deletion or truncation in the unicellular Synechocystis sp...
January 23, 2019: MicrobiologyOpen
Vida Časaitė, Mikas Sadauskas, Justas Vaitekūnas, Renata Gasparavičiūtė, Rita Meškienė, Izabelė Skikaitė, Mantas Sakalauskas, Jevgenija Jakubovska, Daiva Tauraitė, Rolandas Meškys
Here, we present a proof-of-principle for a new high-throughput functional screening of metagenomic libraries for the selection of enzymes with different activities, predetermined by the substrate being used. By this approach, a total of 21 enzyme-coding genes were selected, including members of xanthine dehydrogenase, aldehyde dehydrogenase (ALDH), and amidohydrolase families. The screening system is based on a pro-chromogenic substrate, which is transformed by the target enzyme to indole-3-carboxylic acid...
January 21, 2019: MicrobiologyOpen
Kaoru Arii, Miki Kawada-Matsuo, Yuichi Oogai, Kazuyuki Noguchi, Hitoshi Komatsuzawa
Nisin A is a lantibiotic produced by Lactococcus lactis that is widely used as a food preservative. In Staphylococcus aureus, the BraRS two-component system (TCS) senses nisin A and regulates the expression of the ABC transporter VraDE, which is responsible for nisin A resistance. In this study, we exposed S. aureus to a sub-minimum inhibition concentration of nisin A and obtained three spontaneous mutants that were highly resistant to this lantibiotic, designated as SAN (S. aureus nisin resistant) 1, SAN8, and SAN87...
January 17, 2019: MicrobiologyOpen
Kok-Gan Chan, Wah-Seng See-Too, Kah-Ooi Chua, Álvaro Peix, Kian Mau Goh, Kar-Wai Hong, Wai-Fong Yin, Li-Sin Lee
A bacterial strain designated as P08T was isolated from laboratory tap water during a water quality assessment in University of Malaya, Malaysia. The strain was a Gram-negative, rod-shaped, nonmotile, and aerobic bacterium. Complete genome of P08T comprised of a 2,820,660 bp chromosome with a G + C content of 36.43%. Both 16S rRNA phylogeny and phylogenetic tree inferred from the core gene matrix demonstrated that P08T formed a hitherto unknown subline within the family Neisseriaceae. Ortho average nucleotide identity (OrthoANI) values and the percentage of conserved proteins (POCP) calculated from complete genome sequence indicated low relatedness between P08T and its phylogenetic neighbors...
January 17, 2019: MicrobiologyOpen
Guan-Tang Xu, Han Xue, Chun-Gen Piao, Min-Wei Guo, Yong Li
A Gram-staining positive facultative anaerobic, non-motile strain, sk1b4T , was isolated from canker of symptomatic bark tissue of a Populus × euramericana. 16S rRNA gene sequence analyses showed that strain sk1b4T shared the highest similarity with Arcanobacterium phocisimile (94.1%). Within the phylogenetic tree, the novel isolate formed a distinct branch from Actinobaculum, Arcanobacterium, and Trueperella. The percentage of conserved proteins calculated from genomic sequence indicated a low level of relatedness between the novel strain and its phylogenetic neighbors...
January 17, 2019: MicrobiologyOpen
Anna Nowaczek, Renata Urban-Chmiel, Marta Dec, Andrzej Puchalski, Dagmara Stępień-Pyśniak, Agnieszka Marek, Ewelina Pyzik
Bacteria of the genus Campylobacter are the most common pathogens causing zoonotic diseases in humans. Therefore, the aim of the study was to isolate Campylobacter bacteria from broiler chickens and evaluate their susceptibility to selected antibiotics by determining minimum inhibitory concentrations (MIC), followed by isolation and characterization of bacteriophages specific for Campylobacter spp. The material for the study consisted of field isolates of Campylobacter spp. obtained from the gut (cecum) of broiler chickens directly after slaughter in slaughterhouses, and bacteriophages specific for these strains...
January 17, 2019: MicrobiologyOpen
Bulent Gözel, Camille Monney, Lisandra Aguilar-Bultet, Sebastian Rupp, Joachim Frey, Anna Oevermann
Listeriosis is a severe disease caused by the opportunistic bacterial pathogen Listeria monocytogenes (L. monocytogenes). Previous studies indicate that of the four phylogenetical lineages known, lineage I strains are significantly more prevalent in clinical infections than in the environment. Among lineage 1, sequence type (ST1) belongs to the most frequent genotypes in clinical infections and behaves hyperinvasive in experimental in vitro infections compared to lineage II strains suggesting that yet uncharacterized virulence genes contribute to high virulence of certain lineage I strains...
January 17, 2019: MicrobiologyOpen
Pongdet Piromyou, Pongpan Songwattana, Kamonluck Teamtisong, Panlada Tittabutr, Nantakorn Boonkerd, Piyada Alisha Tantasawat, Eric Giraud, Michael Göttfert, Neung Teaumroong
This study supports the idea that the evolution of type III secretion system (T3SS) is one of the factors that controls Vigna radiata-bradyrhizobia symbiosis. Based on phylogenetic tree data and gene arrangements, it seems that the T3SSs of the Thai bradyrhizobial strains SUTN9-2, DOA1, and DOA9 and the Senegalese strain ORS3257 may share the same origin. Therefore, strains SUTN9-2, DOA1, DOA9, and ORS3257 may have evolved their T3SSs independently from other bradyrhizobia, depending on biological and/or geological events...
January 9, 2019: MicrobiologyOpen
Helena L Spiewak, Sravanthi Shastri, Lili Zhang, Stephan Schwager, Leo Eberl, Annette C Vergunst, Mark S Thomas
Burkholderia cenocepacia is an opportunistic bacterial pathogen that poses a significant threat to individuals with cystic fibrosis by provoking a strong inflammatory response within the lung. It possesses a type VI secretion system (T6SS), a secretory apparatus that can perforate the cellular membrane of other bacterial species and/or eukaryotic targets, to deliver an arsenal of effector proteins. The B. cenocepacia T6SS (T6SS-1) has been shown to be implicated in virulence in rats and contributes toward actin rearrangements and inflammasome activation in B...
January 9, 2019: MicrobiologyOpen
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