Díaz-Palma Paula, Alfaro Gleny, Hengst Martha, Pozo Patricia, Stegen Susana, Queirolo Fabrizio, Rojo Gonzalo, Silva Pedro, Arias Diana, Gallardo Karem, Contreras-Ortega Carlos
BACKGROUND: The purpose of this study was to identify arsenite-oxidizing halobacteria in samples obtained from Salar de Punta Negra, II Region of Chile. Seven bacterial isolates, numbered as isolates I to VII, grown in a culture medium with 100 ppm as NaAsO2 (As (III)) were tested. Bacterial growth kinetics and the percent of arsenite removal (PAR) were performed simultaneously with the detection of an arsenite oxidase enzyme through Dot Blot analysis. RESULTS: An arsenite oxidase enzyme was detected in all isolates, expressed constitutively after 10 generations grown in the absence of As (III)...
2013: Aquatic Biosystems