journal
https://read.qxmd.com/read/38907996/an-ipsc-derived-small-intestine-on-chip-with-self-organizing-epithelial-mesenchymal-and-neural-cells
#1
JOURNAL ARTICLE
Renée Moerkens, Joram Mooiweer, Aarón D Ramírez-Sánchez, Roy Oelen, Lude Franke, Cisca Wijmenga, Robert J Barrett, Iris H Jonkers, Sebo Withoff
Human induced pluripotent stem cell (hiPSC)-derived intestinal organoids are valuable tools for researching developmental biology and personalized therapies, but their closed topology and relative immature state limit applications. Here, we use organ-on-chip technology to develop a hiPSC-derived intestinal barrier with apical and basolateral access in a more physiological in vitro microenvironment. To replicate growth factor gradients along the crypt-villus axis, we locally expose the cells to expansion and differentiation media...
June 20, 2024: Cell Reports
https://read.qxmd.com/read/38905102/neocortical-inhibitory-imbalance-predicts-successful-sensory-detection
#2
JOURNAL ARTICLE
Christopher A Deister, Alexander I Moore, Jakob Voigts, Sophia Bechek, Rebecca Lichtin, Tyler C Brown, Christopher I Moore
Perceptual success depends on fast-spiking, parvalbumin-positive interneurons (FS/PVs). However, competing theories of optimal rate and correlation in pyramidal (PYR) firing make opposing predictions regarding the underlying FS/PV dynamics. We addressed this with population calcium imaging of FS/PVs and putative PYR neurons during threshold detection. In primary somatosensory and visual neocortex, a distinct PYR subset shows increased rate and spike-count correlations on detected trials ("hits"), while most show no rate change and decreased correlations...
June 20, 2024: Cell Reports
https://read.qxmd.com/read/38905100/dot1l-h3k79me2-represses-hiv-1-reactivation-via-recruiting-dcaf1
#3
JOURNAL ARTICLE
Fenfei Liang, Jiaxing Jin, Qiming Li, Jiangkai Duan, Ao Jiang, Xiaoqing Chen, Huichao Geng, Kai Wu, Fei Yu, Xiaolu Zhao, Yu Zhou, Deqing Hu, Liang Chen
DOT1L mediates the methylation of histone H3 at lysine 79 and, in turn, the transcriptional activation or repression in a context-dependent manner, yet the regulatory mechanisms and functions of DOT1L/H3K79me remain to be fully explored. Following peptide affinity purification and proteomic analysis, we identified that DCAF1-a component of the E3 ligase complex involved in HIV regulation-is associated with H3K79me2 and DOT1L. Interestingly, blocking the expression or catalytic activity of DOT1L or repressing the expression of DCAF1 significantly enhances the tumor necrosis factor alpha (TNF-α)/nuclear factor κB (NF-κB)-induced reactivation of the latent HIV-1 genome...
June 20, 2024: Cell Reports
https://read.qxmd.com/read/38905101/retrograde-adenosine-a-2a-receptor-signaling-facilitates-excitatory-synaptic-transmission-and-seizures
#4
JOURNAL ARTICLE
Kaoutsar Nasrallah, Coralie Berthoux, Yuki Hashimotodani, Andrés E Chávez, Michelle C Gulfo, Rafael Luján, Pablo E Castillo
Retrograde signaling at the synapse is a fundamental way by which neurons communicate and neuronal circuit function is fine-tuned upon activity. While long-term changes in neurotransmitter release commonly rely on retrograde signaling, the mechanisms remain poorly understood. Here, we identified adenosine/A2A receptor (A2A R) as a retrograde signaling pathway underlying presynaptic long-term potentiation (LTP) at a hippocampal excitatory circuit critically involved in memory and epilepsy. Transient burst activity of a single dentate granule cell induced LTP of mossy cell synaptic inputs, a BDNF/TrkB-dependent form of plasticity that facilitates seizures...
June 19, 2024: Cell Reports
https://read.qxmd.com/read/38900641/spatial-proteomics-of-skeletal-muscle-using-thin-cryosections-reveals-metabolic-adaptation-at-the-muscle-tendon-transition-zone
#5
JOURNAL ARTICLE
Luisa Schmidt, Michael Saynisch, Christian Hoegsbjerg, Andreas Schmidt, Abigail Mackey, Jan-Wilm Lackmann, Stefan Müller, Manuel Koch, Bent Brachvogel, Michael Kjaer, Philipp Antczak, Marcus Krüger
Morphological studies of skeletal muscle tissue provide insights into the architecture of muscle fibers, the surrounding cells, and the extracellular matrix (ECM). However, a spatial proteomics analysis of the skeletal muscle including the muscle-tendon transition zone is lacking. Here, we prepare cryotome muscle sections of the mouse soleus muscle and measure each slice using short liquid chromatography-mass spectrometry (LC-MS) gradients. We generate 3,000 high-resolution protein profiles that serve as the basis for a network analysis to reveal the complex architecture of the muscle-tendon junction...
June 19, 2024: Cell Reports
https://read.qxmd.com/read/38900638/the-condensation-of-hp1%C3%AE-swi6-imparts-nuclear-stiffness
#6
JOURNAL ARTICLE
Jessica F Williams, Ivan V Surovtsev, Sarah M Schreiner, Ziyuan Chen, Gulzhan Raiymbek, Hang Nguyen, Yan Hu, Julie S Biteen, Simon G J Mochrie, Kaushik Ragunathan, Megan C King
Biomolecular condensates have emerged as major drivers of cellular organization. It remains largely unexplored, however, whether these condensates can impart mechanical function(s) to the cell. The heterochromatin protein HP1α (Swi6 in Schizosaccharomyces pombe) crosslinks histone H3K9 methylated nucleosomes and has been proposed to undergo condensation to drive the liquid-like clustering of heterochromatin domains. Here, we leverage the genetically tractable S. pombe model and a separation-of-function allele to elucidate a mechanical function imparted by Swi6 condensation...
June 19, 2024: Cell Reports
https://read.qxmd.com/read/38900636/insulin-and-leptin-oscillations-license-food-entrained-browning-and-metabolic-flexibility
#7
JOURNAL ARTICLE
Pamela Mattar, Andressa Reginato, Christian Lavados, Debajyoti Das, Manu Kalyani, Nuria Martinez-Lopez, Mridul Sharma, Grethe Skovbjerg, Jacob Lercke Skytte, Urmas Roostalu, Rajasekaran Subbarayan, Elodie Picarda, Xingxing Zang, Jinghang Zhang, Chandan Guha, Gary Schwartz, Prashant Rajbhandari, Rajat Singh
Timed feeding drives adipose browning, although the integrative mechanisms for the same remain unclear. Here, we show that twice-a-night (TAN) feeding generates biphasic oscillations of circulating insulin and leptin, representing their entrainment by timed feeding. Insulin and leptin surges lead to marked cellular, functional, and metabolic remodeling of subcutaneous white adipose tissue (sWAT), resulting in increased energy expenditure. Single-cell RNA-sequencing (scRNA-seq) analyses and flow cytometry demonstrate a role for insulin and leptin surges in innate lymphoid type 2 (ILC2) cell recruitment and sWAT browning, since sWAT depot denervation or loss of leptin or insulin receptor signaling or ILC2 recruitment each dampens TAN feeding-induced sWAT remodeling and energy expenditure...
June 19, 2024: Cell Reports
https://read.qxmd.com/read/38900640/a-pre-vaccination-immune-metabolic-interplay-determines-the-protective-antibody-response-to-a-dengue-virus-vaccine
#8
JOURNAL ARTICLE
Adam-Nicolas Pelletier, Gabriela Pacheco Sanchez, Abdullah Izmirly, Mark Watson, Tiziana Di Pucchio, Karina Inacio Carvalho, Abdelali Filali-Mouhim, Eustache Paramithiotis, Maria do Carmo S T Timenetsky, Alexander Roberto Precioso, Jorge Kalil, Michael S Diamond, Elias K Haddad, Esper G Kallas, Rafick Pierre Sekaly
Protective immunity to dengue virus (DENV) requires antibody response to all four serotypes. Systems vaccinology identifies a multi-OMICs pre-vaccination signature and mechanisms predictive of broad antibody responses after immunization with a tetravalent live attenuated DENV vaccine candidate (Butantan-DV/TV003). Anti-inflammatory pathways, including TGF-β signaling expressed by CD68low monocytes, and the metabolites phosphatidylcholine (PC) and phosphatidylethanolamine (PE) positively correlate with broadly neutralizing antibody responses against DENV...
June 18, 2024: Cell Reports
https://read.qxmd.com/read/38900639/hypoxia-induced-ctcf-promotes-emt-in-breast-cancer
#9
JOURNAL ARTICLE
Parik Kakani, Shruti Ganesh Dhamdhere, Deepak Pant, Rushikesh Joshi, Jharna Mishra, Atul Samaiya, Sanjeev Shukla
Cancer cells experiencing hypoxic stress employ epithelial-mesenchymal transition (EMT) to undergo metastasis through rewiring of the chromatin landscape, epigenetics, and importantly, gene expression. Here, we showed that hypoxia modulates the epigenetic landscape on CTCF promoter and upregulates its expression. Hypoxia-driven epigenetic regulation, specifically DNA demethylation mediated by TET2, is a prerequisite for CTCF induction. Mechanistically, in hypoxic conditions, Hypoxia-inducible factor 1-alpha (HIF1α) binds to the unmethylated CTCF promoter, causing transcriptional upregulation...
June 18, 2024: Cell Reports
https://read.qxmd.com/read/38900637/transcriptional-determinism-and-stochasticity-contribute-to-the-complexity-of-autism-associated-shank-family-genes
#10
JOURNAL ARTICLE
Xiaona Lu, Pengyu Ni, Paola Suarez-Meade, Yu Ma, Emily Niemitz Forrest, Guilin Wang, Yi Wang, Alfredo Quiñones-Hinojosa, Mark Gerstein, Yong-Hui Jiang
Precision of transcription is critical because transcriptional dysregulation is disease causing. Traditional methods of transcriptional profiling are inadequate to elucidate the full spectrum of the transcriptome, particularly for longer and less abundant mRNAs. SHANK3 is one of the most common autism causative genes. Twenty-four Shank3-mutant animal lines have been developed for autism modeling. However, their preclinical validity has been questioned due to incomplete Shank3 transcript structure. We apply an integrative approach combining cDNA-capture and long-read sequencing to profile the SHANK3 transcriptome in humans and mice...
June 18, 2024: Cell Reports
https://read.qxmd.com/read/38900635/tonic-type-2-immunity-is-a-critical-tissue-checkpoint-controlling-autoimmunity-in-the-skin
#11
JOURNAL ARTICLE
Jeong-Eun Lee, Mina Kim, Sotaro Ochiai, Sung-Hee Kim, Hyeonuk Yeo, Jahyun Bok, Jiyeon Kim, Miso Park, Daehong Kim, Olivier Lamiable, Myunggyo Lee, Min-Ju Kim, Hye Young Kim, Franca Ronchese, Sung Won Kwon, Haeseung Lee, Tae-Gyun Kim, Yeonseok Chung
Immunoregulatory mechanisms established in the lymphoid organs are vital for preventing autoimmunity. However, the presence of similar mechanisms in non-lymphoid tissues remains unclear. Through transcriptomic and lipidomic analyses, we find a negative association between psoriasis and fatty acid metabolism, as well as Th2 signature. Homeostatic expression of liver X receptor (LXR) and peroxisome proliferator-activated receptor gamma (PPARγ) is essential for maintaining fatty acid metabolism and for conferring resistance to psoriasis in mice...
June 18, 2024: Cell Reports
https://read.qxmd.com/read/38900634/excitatory-and-inhibitory-synapses-show-a-tight-subcellular-correlation-that-weakens-over-development
#12
JOURNAL ARTICLE
Sally Horton, Vincenzo Mastrolia, Rachel E Jackson, Sarah Kemlo, Pedro M Pereira Machado, Maria Alejandra Carbajal, Robert Hindges, Roland A Fleck, Paulo Aguiar, Guilherme Neves, Juan Burrone
Neurons receive correlated levels of excitation and inhibition, a feature that is important for proper brain function. However, how this relationship between excitatory and inhibitory inputs is established during the dynamic period of circuit wiring remains unexplored. Using multiple techniques, including in utero electroporation, electron microscopy, and electrophysiology, we reveal a tight correlation in the distribution of excitatory and inhibitory synapses along the dendrites of developing CA1 hippocampal neurons...
June 18, 2024: Cell Reports
https://read.qxmd.com/read/38896777/variant-specific-antibody-response-following-repeated-sars-cov-2-vaccination-and-infection
#13
JOURNAL ARTICLE
Xiao-Lin Jiang, Xue-Dong Song, Chao Shi, Guo-Jian Yang, Xue-Jun Wang, Yu-Wei Zhang, Jie Wu, Lian-Xiang Zhao, Ming-Zhu Zhang, Ming-Ming Wang, Rui-Rui Chen, Xue-Juan He, Er-Hei Dai, Hui-Xia Gao, Yuan Shen, Gang Dong, Yu-Ling Wang, Mai-Juan Ma
The ongoing emergence of SARS-CoV-2 variants poses challenges to the immunity induced by infections and vaccination. We conduct a 6-month longitudinal evaluation of antibody binding and neutralization of sera from individuals with six different combinations of vaccination and infection against BA.5, XBB.1.5, EG.5.1, and BA.2.86. We find that most individuals produce spike-binding IgG or neutralizing antibodies against BA.5, XBB.1.5, EG.5.1, and BA.2.86 2 months after infection or vaccination. However, compared to ancestral strain and BA...
June 18, 2024: Cell Reports
https://read.qxmd.com/read/38906147/fibrinopeptide-a-induced-blood-feeding-arrest-in-the-yellow-fever-mosquito-aedes-aegypti
#14
JOURNAL ARTICLE
Chisako Sakuma, Takeo Iwamoto, Keiko Masuda, Yoshihiro Shimizu, Fumiaki Obata, Hirotaka Kanuka
Female mosquitoes engage in blood feeding from their hosts to facilitate egg maturation but cease feeding once a sufficient blood meal has been acquired. Abdominal distention has been proposed as a contributing factor; however, it has also been suggested that there are chemical controls. In this study, we focus on negative chemical regulators of blood feeding, particularly those present in the host blood. Serum derived from animal blood inhibits the feeding of ATP, a phagostimulant of blood feeding in Aedes aegypti...
June 17, 2024: Cell Reports
https://read.qxmd.com/read/38889007/an-enhancer-rna-recruits-kmt2a-to-regulate-transcription-of-myb
#15
JOURNAL ARTICLE
Juhyun Kim, Luis F Diaz, Matthew J Miller, Benjamin Leadem, Ivan Krivega, Ann Dean
The Myb proto-oncogene encodes the transcription factor c-MYB, which is critical for hematopoiesis. Distant enhancers of Myb form a hub of interactions with the Myb promoter. We identified a long non-coding RNA (Myrlin) originating from the -81-kb murine Myb enhancer. Myrlin and Myb are coordinately regulated during erythroid differentiation. Myrlin TSS deletion using CRISPR-Cas9 reduced Myrlin and Myb expression and LDB1 complex occupancy at the Myb enhancers, compromising enhancer contacts and reducing RNA Pol II occupancy in the locus...
June 17, 2024: Cell Reports
https://read.qxmd.com/read/38889006/the-pp2a-regulatory-subunit-ppp2r2a-controls-nad-biosynthesis-to-regulate-t%C3%A2-cell-subset-differentiation-in-systemic-autoimmunity
#16
JOURNAL ARTICLE
Wenliang Pan, Maria G Tsokos, Marc Scherlinger, Wei Li, George C Tsokos
The protein phosphatase 2A (PP2A) regulatory subunit PPP2R2A is involved in the regulation of immune response. We report that lupus-prone mice with T cells deficient in PPP2R2A display less autoimmunity and nephritis. PPP2R2A deficiency promotes NAD+ biosynthesis through the nicotinamide riboside (NR)-directed salvage pathway in T cells. NR inhibits murine Th17 and promotes Treg cell differentiation, in vitro, by PΑRylating histone H1.2 and causing its reduced occupancy in the Foxp3 loci and increased occupancy in the Il17a loci, leading to increased Foxp3 and decreased Il17a transcription...
June 17, 2024: Cell Reports
https://read.qxmd.com/read/38889005/antibody-activation-of-connexin-hemichannels-in-bone-osteocytes-with-atp-release-suppresses-breast-cancer-and-osteosarcoma-malignancy
#17
JOURNAL ARTICLE
Manuel A Riquelme, Xuewei Wang, Francisca M Acosta, Jingruo Zhang, Jeffery Chavez, Sumin Gu, Peng Zhao, Wei Xiong, Ningyan Zhang, Guo Li, Saranya Srinivasan, Chaoyu Ma, Manjeet K Rao, Lu-Zhe Sun, Nu Zhang, Zhiqiang An, Jean X Jiang
Bone tissue represents the most frequent site of cancer metastasis. We developed a hemichannel-activating antibody, Cx43-M2. Cx43-M2, directly targeting osteocytes in situ, activates osteocytic hemichannels and elevates extracellular ATP, thereby inhibiting the growth and migration of cultured breast and osteosarcoma cancer cells. Cx43-M2 significantly decreases breast cancer metastasis, osteosarcoma growth, and osteolytic activity, while improving survival rates in mice. The antibody's inhibition of breast cancer and osteosarcoma is dose dependent in both mouse and human cancer metastatic models...
June 17, 2024: Cell Reports
https://read.qxmd.com/read/38879877/the-phosphorylation-deubiquitination-positive-feedback-loop-of-the-chk2-usp7-axis-stabilizes-p53-under-oxidative-stress
#18
JOURNAL ARTICLE
Jingwei Liu, Liangzi Cao, Yubang Wang, Yu Zou, Qiqiang Guo, Shu Chen, Bo Jiang, Xuan Wu, Lixia Zheng, Siyi Zhang, Songming Lu, Keshen Zhou, Pengcheng Jiang, Yutong Xiao, Ruohan Yang, Shiyuan Dong, Ziwei Li, Di Chen, Ying Zhang, Naijin Zhang, Guozhe Sun, Chengzhong Xing, Xiaoyu Song, Zhenning Wang, Liu Cao
p53 regulates multiple signaling pathways and maintains cell homeostasis under conditions of DNA damage and oxidative stress. Although USP7 has been shown to promote p53 stability via deubiquitination, the USP7-p53 activation mechanism has remained unclear. Here, we propose that DNA damage induces reactive oxygen species (ROS) production and activates ATM-CHK2, and CHK2 then phosphorylates USP7 at S168 and T231. USP7 phosphorylation is essential for its deubiquitination activity toward p53. USP7 also deubiquitinates CHK2 at K119 and K131, increasing CHK2 stability and creating a positive feedback loop between CHK2 and USP7...
June 14, 2024: Cell Reports
https://read.qxmd.com/read/38878291/molecular-landscape-of-the-ribosome-pre-initiation-complex-during-mrna-scanning-structural-role-for-eif3c-and-its-control-by-eif5
#19
Eiji Obayashi, Rafael E Luna, Takashi Nagata, Pilar Martin-Marcos, Hiroyuki Hiraishi, Chingakham Ranjit Singh, Jan Peter Erzberger, Fan Zhang, Haribabu Arthanari, Jacob Morris, Riccardo Pellarin, Chelsea Moore, Ian Harmon, Evangelos Papadopoulos, Hisashi Yoshida, Mahmoud L Nasr, Satoru Unzai, Bryttney Thompson, Eric Aube, Samantha Hustak, Florian Stengel, Eddie Dagraca, Asokan Ananbandam, Philip Gao, Takeshi Urano, Alan G Hinnebusch, Gerhard Wagner, Katsura Asano
No abstract text is available yet for this article.
June 14, 2024: Cell Reports
https://read.qxmd.com/read/38878290/dorsal-raphe-neurons-integrate-the-values-of-reward-amount-delay-and-uncertainty-in-multi-attribute-decision-making
#20
JOURNAL ARTICLE
Yang-Yang Feng, Ethan S Bromberg-Martin, Ilya E Monosov
The dorsal raphe nucleus (DRN) is implicated in psychiatric disorders that feature impaired sensitivity to reward amount, impulsivity when facing reward delays, and risk-seeking when confronting reward uncertainty. However, it has been unclear whether and how DRN neurons signal reward amount, reward delay, and reward uncertainty during multi-attribute value-based decision-making, where subjects consider these attributes to make a choice. We recorded DRN neurons as monkeys chose between offers whose attributes, namely expected reward amount, reward delay, and reward uncertainty, varied independently...
June 14, 2024: Cell Reports
journal
journal
43845
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.