Tae-Shin Kim, Inseong Oh, Yu Jung Choi, Minjeong Nam, Hajeong Lee, Eun Young Song
BACKGROUND: Pronase pretreatment can reduce rituximab (RTX) interference by degrading CD20 in B-cell flow cytometry crossmatch (FCXM) testing. However, it may also reduce the assay sensitivity by degrading HLA molecules. We investigated the effects of various pronase concentrations on RTX interference and the analytical sensitivity of B-cell FCXM testing. METHODS: Using 59 patient serum samples and 38 donor lymphocyte samples, we designed 97 recipient-donor pairs and divided them into three groups according to RTX use and the presence of weak-to-moderate donor HLA-specific antibody (DSA) reactions: RTX+/DSA-, RTX+/DSA+, and RTX-/DSA+...
July 12, 2024: Annals of Laboratory Medicine
Hyeoun-Ae Park
No abstract text is available yet for this article.
July 3, 2024: Annals of Laboratory Medicine
Shinae Yu, Byung Ryul Jeon, Changseung Liu, Dokyun Kim, Hae-Il Park, Hyung Doo Park, Jeong Hwan Shin, Jun Hyung Lee, Qute Choi, Sollip Kim, Yeo Min Yun, Eun-Jung Cho
BACKGROUND: Healthcare 4.0. refers to the integration of advanced technologies, such as artificial intelligence (AI) and big data analysis, into the healthcare sector. Recognizing the impact of Healthcare 4.0 technologies in laboratory medicine (LM), we seek to assess the overall awareness and implementation of Healthcare 4.0 among members of the Korean Society for Laboratory Medicine (KSLM). METHODS: A web-based survey was conducted using an anonymous questionnaire...
July 2, 2024: Annals of Laboratory Medicine
Jianping Zhang, Jing Zhao, Qingtao Wang, Rui Zhang, Yuhong Yue
BACKGROUND: The accurate measurement of α-fetoprotein (AFP) is critical for clinical diagnosis. However, different AFP immunoassays may yield different results. Appropriate AFP reference materials (RMs) were selected and assigned accurate values for applications with external quality assessment (EQA) programs to standardize AFP measurements. METHODS: Forty individual clinical samples and six different concentrations of candidate RMs (Can-RMs, L1-L6) were prepared by the Beijing Center for Clinical Laboratories...
July 2, 2024: Annals of Laboratory Medicine
Sollip Kim, Tae-Dong Jeong, Kyunghoon Lee, Jae-Woo Chung, Eun-Jung Cho, Seunghoo Lee, Sail Chun, Junghan Song, Won-Ki Min
BACKGROUND: In recent decades, the analytical quality of clinical laboratory results has substantially increased because of collaborative efforts. To effectively utilize laboratory results in applications, such as machine learning through big data, understanding the level of harmonization for each test would be beneficial. We aimed to develop a quantitative harmonization index that reflects the harmonization status of real-world laboratory tests. METHODS: We collected 2021-2022 external quality assessment (EQA) results for eight tests (HbA1c, creatinine, total cholesterol, HDL-cholesterol, triglyceride, alpha-fetoprotein [AFP], carcinoembryonic antigen [CEA], and prostate-specific antigen [PSA])...
June 26, 2024: Annals of Laboratory Medicine
Hyeyoung Lee, Hyunhye Kang, Hyojin Chae, Eun-Jee Oh
Cardiac biomarkers, especially high-sensitivity cardiac troponin C or I (hs-cTnC or hs-cTnI, respectively), are vital for diagnosing acute myocardial infarction (AMI). Despite the specificity of hs-cTn as a biomarker, the creatine kinase-myocardial band (CK-MB) is commonly used alongside hs-cTn in emergency departments (EDs). We analyzed 23,771 simultaneous hs-cTn (hs-cTnT or hs-cTnI) and CK-MB requests for 17,185 patients in tertiary hospital ED in 2022. The objective of this study was to assess their practical value in diagnosing AMI in real-world settings...
June 24, 2024: Annals of Laboratory Medicine
Gun Tak Lee, Byuk Sung Ko, Da Seul Kim, Minha Kim, Jong Eun Park, Sung Yeon Hwang, Daun Jeong, Chi Ryang Chung, Hyunggoo Kang, Jaehoon Oh, Tae Ho Lim, Bora Chae, Won Young Kim, Tae Gun Shin
BACKGROUND: Lactate is a commonly used biomarker for sepsis, although it has limitations in certain cases, suggesting the need for novel biomarkers. We evaluated the diagnostic accuracy of plasma renin concentration and renin activity for mortality and kidney outcomes in patients with sepsis with hypoperfusion or hypotension. METHODS: This was a multicenter, prospective, observational study of 117 patients with septic shock treated at three tertiary emergency departments between September 2021 and October 2022...
June 24, 2024: Annals of Laboratory Medicine
Young Kyu Min, Jae Kyung Kim, Kyung Sun Park, Jong-Won Kim
BACKGROUND: Droplet digital (dd)PCR is a new-generation PCR technique with high precision and sensitivity; however, the positive and negative droplets are not always effectively separated because of the "rain" phenomenon. We aimed to develop a practical optimization and evaluation process for the ddPCR assay and to apply it to the detection of BRAF V600E in fine-needle aspiration (FNA) specimens of thyroid nodules, as an example. METHODS: We optimized seven ddPCR parameters that can affect "rain...
June 14, 2024: Annals of Laboratory Medicine
Seung-Tae Lee
No abstract text is available yet for this article.
June 7, 2024: Annals of Laboratory Medicine
Xincen Duan, Minglong Zhang, Yan Liu, Wenbo Zheng, Chun Yee Lim, Sollip Kim, Tze Ping Loh, Wei Guo, Rui Zhou, Tony Badrick
Patient-based real-time QC (PBRTQC) uses patient-derived data to assess assay performance. PBRTQC algorithms have advanced in parallel with developments in computer science and the increased availability of more powerful computers. The uptake of Artificial Intelligence in PBRTQC has been rapid, with many stated advantages over conventional approaches. However, until this review, there has been no critical comparison of these. The PBRTQC algorithms based on moving averages, regression-adjusted real-time QC, neural networks and anomaly detection are described and contrasted...
June 5, 2024: Annals of Laboratory Medicine
Paola Concolino, Elisa De Paolis, Martina Rinelli, Giulia Maneri, Francesca Brisighelli, Rita Trozzi, Simona Duranti, Luciano Giacò, Maria Piane, Alessia Preziosi, Arianna Panfili, Giovanni Scambia, Camilla Nero, Maria De Bonis, Angelo Minucci
No abstract text is available yet for this article.
May 31, 2024: Annals of Laboratory Medicine
Young Ah Kim
No abstract text is available yet for this article.
May 30, 2024: Annals of Laboratory Medicine
Jiwon Yun, Young Chul Youn, Hye Ryoun Kim
Few studies have focused on the association between clonal hematopoiesis of indeterminate potential (CHIP) and β-amyloid (Aβ) deposition in the brain, which causes Alzheimer's disease. We aimed to investigate the potential role of CHIP in brain Aβ deposition in Korean patients. We enrolled 58 Korean patients over 50 yrs of age with cognitive impairment who underwent brain Aβ positron emission tomography. We explored CHIP in their peripheral blood using deep-targeted next-generation sequencing...
May 28, 2024: Annals of Laboratory Medicine
Yeeun Shim, Jieun Seo, Seung-Tae Lee, Jong Rak Choi, Young-Chul Choi, Saeam Shin, Hyung Jun Park
BACKGROUND: Facioscapulohumeral muscular dystrophy (FSHD) is a common form of muscular dystrophy that mainly affects skeletal muscle. FSHD1 accounts for 95% of all FSHD cases and can be diagnosed based on the pathogenic contraction of the D4Z4-repeat array on chromosome 4q35. Genetic diagnosis of FSHD1 is challenging because of the large size and repetitive nature of the D4Z4 region. We evaluated the clinical applicability of optical genome mapping (OGM) for the genetic diagnosis of FSHD1...
May 10, 2024: Annals of Laboratory Medicine
Zhenyi Huang, Ziyan Li, Yating Li, Yunshan Cao, Suping Zhong, Jinlu Liu, Zhiqian Lin, Lijuan Lin, Yanping Fang, Jing Zeng, Zhaoying Su, Huibin Li, Jianfen Liang, Biqing Zhu, Zipei Lin, Yongxin Huang, Xuexi Yang, Lingxiao Jiang
BACKGROUND: Quantitative detection of glucose-6-phosphate dehydrogenase (G6PD) is commonly done to screen for G6PD deficiency. However, current reference intervals (RIs) of G6PD are unsuitable for evaluating G6PD-activity levels with local populations or associating G6PD variants with hemolysis risk to aid clinical decision-making. We explored appropriate RIs and clinical decision limits (CDLs) for G6PD activity in individuals from Guangzhou, China. METHODS: We enrolled 5,852 unrelated individuals between 2020 and 2022 and screened their samples in quantitative assays for G6PD activity...
May 3, 2024: Annals of Laboratory Medicine
Eun Ji Oh, Hyun Hee Jang, Sangwon Park, Hyun-Pil Lim, Kwi-Dug Yun, Woohyung Jang, Ok-Su Kim, Chan Park, Eun Jeong Won
No abstract text is available yet for this article.
April 22, 2024: Annals of Laboratory Medicine
Sung Hyun Cho, Yong Jun Kwon, Seung A Byun, Jong Hee Shin, Hyun-Woo Choi, Suk Hee Heo, Jun-Hyuk Lim, Myung-Sun Kim, Yoonjung Lee, Seung Ji Kang
No abstract text is available yet for this article.
April 19, 2024: Annals of Laboratory Medicine
Suhyeon Woo, Bohyun Kim, Nam Hun Heo, Min-Sun Kim, Young Ahn Yoon, Young-Jin Choi
Pseudothrombocytopenia caused by platelet clumping (PC) can lead to unnecessary platelet transfusions or underdiagnosis of hematologic neoplasms. To overcome these limitations, we assessed the capacity of the Sysmex DI-60 digital morphology analyzer (Sysmex, Kobe, Japan) for detecting PC and determining an accurate platelet count in the presence of PC. For this purpose, 135 samples with or without PC (groups Y and N, respectively) were processed by an examiner (a hematologic specialist) using both the Sysmex XN-9000 and DI-60 analyzers...
April 19, 2024: Annals of Laboratory Medicine
Dae-Hyun Kim, Byung-Cheol Kwak, Byeol-A Yoon, Jae-Kwan Cha, Jong-Sung Park, Min-Sun Kwak, Kwang-Sook Woo, Jin-Yeong Han
No abstract text is available yet for this article.
April 18, 2024: Annals of Laboratory Medicine
Weiqiang Chen, Peiling Yu, Chao Chen, Shaoyan Cai, Junheng Chen, Chunqin Zheng, Chaojin Chen, Liangjie Zheng, Chunming Guo
BACKGROUND: Millions of patients undergo cardiac surgery each year. The red blood cell distribution width (RDW) could help predict the prognosis of patients who undergo percutaneous coronary intervention or coronary artery bypass surgery. We investigated whether the RDW has robust predictive value for the 30-day mortality among patients in an intensive care unit (ICU) after undergoing cardiac surgery. METHODS: Using the Medical Information Mart for Intensive Care-IV Database, we retrieved data for 11,634 patients who underwent cardiac surgery in an ICU...
September 1, 2024: Annals of Laboratory Medicine
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