Yoichi Ishitsuka, Yusuke Fukumoto, Yuki Kondo, Mitsuru Irikura, Daisuke Kadowaki, Yuki Narita, Sumio Hirata, Hiroshi Moriuchi, Toru Maruyama, Naotaka Hamasaki, Tetsumi Irie
We evaluated the usefulness of phosphoenolpyruvate (PEP), a glycolytic intermediate with antioxidative and energy supplementation potentials, as an organ preservation agent. Using ex vivo mouse liver and kidney of a static cold storage model, we compared the effects of PEP against organ damage and oxidative stress during cold preservation with those of glucose or N-acetylcysteine (NAC). Lactate dehydrogenase (LDH) leakage, histological changes, and oxidative stress parameters (measured as thiobarbituric acid reactive substance and glutathione content) were determined...
2013: ISRN Pharmacology