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3 Biotech

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https://read.qxmd.com/read/30729093/species-specific-loop-mediated-isothermal-amplification-lamp-assay-for-identification-of-tissue-of-cattle-origin-by-targeting-mitochondrial-gene-sequences
#1
Sarita Kumari, Rajiv Ranjan Kumar, Sanjod Kumar Mendiratta, Deepak Kumar, Preeti Rana, Dhananjay Kumar, Jyoti Jawla
The present study was carried out with the objective of development of species-specific loop-mediated isothermal amplification (LAMP) assay for identification of tissue of cattle origin. The cattle-specific LAMP primer set was designed by targeting mitochondrial D-loop gene. The conditions for LAMP reaction for amplification of template DNA from cattle using designed cattle-specific primer set were optimized for the components of mixture and temperature of reaction. Amplified products were analysed using SYBR Green I dye and by agarose gel electrophoresis...
March 2019: 3 Biotech
https://read.qxmd.com/read/30729092/nanomaterials-and-microbes-interactions-a-contemporary-overview
#2
REVIEW
Jaspreet Singh, Kanchan Vishwakarma, Naleeni Ramawat, Padmaja Rai, Vivek Kumar Singh, Rohit Kumar Mishra, Vivek Kumar, Durgesh Kumar Tripathi, Shivesh Sharma
Use of nanomaterials in the field of science and technology includes different fields in food industry, medicine, agriculture and cosmetics. Nanoparticle-based sensors have wide range of applications in food industry for identification and detection of chemical contaminants, pathogenic bacteria, toxins and fungal toxins from food materials with high specificity and sensitivity. Nanoparticle-microbe interactions play a significant role in disease treatment in the form of antimicrobial agents. The inhibitory mechanism of nanoparticles against different bacteria and fungi includes release of metal ions that interacts with cellular components through various pathways including reactive oxygen species (ROS) generation, pore formation in cell membranes, cell wall damage, DNA damage, and cell cycle arrest and ultimately inhibits the growth of cells...
March 2019: 3 Biotech
https://read.qxmd.com/read/30729091/low-cost-shoot-multiplication-and-improved-growth-in-different-cultivars-of-canna-indica
#3
Dharmendra Kumar Purshottam, Ramesh Kumar Srivastava, Pratibha Misra
Canna ( Canna indica L.) is an ornamental landscape plant used specially for the garden borders and beds. It grows in tropical and subtropical countries including India. Canna is a less explored crop, mainly because it is a slow growing monocot with extremely hard seed coat and difficult to establish in vitro, as bacterial contamination is carried through the soil-grown rhizome. Many cultivars (ca. 150) of canna are being maintained in the garden germplasm of National Botanical Research Institute. To obtain 100% in vitro seed germination, chipping off of seeds with a sterilized nail clipper and soaking for 24-48 h or until radical emergence was a prerequisite...
March 2019: 3 Biotech
https://read.qxmd.com/read/30729090/urinary-n-acetyl-beta-d-glucosaminidase-nag-with-neutrophil-gelatinase-associated-lipocalin-ngal-improves-the-diagnostic-value-for-proximal-tubule-damage-in-diabetic-kidney-disease
#4
Khalid Siddiqui, Basim Al-Malki, Teena Puthiyaparampil George, Shaik Sarfaraz Nawaz, Khalid Al Rubeaan
Screening for diabetic kidney disease (DKD) remains a challenge; however, there has been an ongoing research to investigate the diagnostic value of different biomarkers to identify DKD. The aim of this study was to assess the diagnostic value of both N -acetyl-beta-d-glucosaminidase (NAG) and neutrophil gelatinase-associated lipocalin (NGAL) in the progression of DKD. This cross-sectional case-control study included 92 type 2 diabetic patients with or without DKD. Urinary NAG and NGAL were measured to evaluate their diagnostic values as biochemical markers related to DKD...
March 2019: 3 Biotech
https://read.qxmd.com/read/30729089/association-of-a-candidatus-phytoplasma-aurantifolia-related-strain-with-apricot-showing-european-stone-fruit-yellows-symptoms-in-iran
#5
Rasoul Rasoulpour, Mohammad Salehi, Assunta Bertaccini
During 2012-2015 surveys in some orchards in Faraghe (Iran), a number of apricot trees showed symptoms resembling those associated with the phytoplasma disease known as European stone fruit yellows that are severe leaf roll, yellowing and die back. The presence of an infectious agent was confirmed by graft transmission experiments in which all the previously symptomless GF-677 (peach × almond) trees showed phytoplasma-type symptoms. The phytoplasma presence was confirmed by nested PCR assays using DNA extracted from samples from both field collected and graft-inoculated trees...
March 2019: 3 Biotech
https://read.qxmd.com/read/30729088/in-vivo-and-in-vitro-effects-on-cholinesterase-of-blood-of-oreochromis-mossambicus-by-copper
#6
Ain Aqilah Basirun, Siti Aqlima Ahmad, Mohd Khalizan Sabullah, Nur Adeela Yasid, Hassan Mohd Daud, Ariff Khalid, Mohd Yunus Shukor
The present study is aimed to evaluate the effects of sub-acute toxicity testing of copper sulphate (CuSO4 ), on behavioural, histological and biochemical changes of the Oreochromis mossambicus (black tilapia) blood tissues. The effects were assessed according to the previous results on sub-acute toxicity test after exposing fish to several concentrations (0.0, 2.5, 5.0, and 10.0 mg/L). The observations of scanning electron microscope, and transmission electron microscope studies revealed severe histopathological changes on the surface and the cellular changes in blood tissues, respectively...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729087/the-draft-genome-sequence-of-clostridium-sp-strain-ct7-an-isolate-capable-of-producing-butanol-but-not-acetone-and-1-3-propanediol-from-crude-glycerol
#7
Jiasheng Lu, Tianpeng Chen, Yujia Jiang, Wenming Zhang, Weiliang Dong, Jie Zhou, Jiangfeng Ma, Yan Fang, Min Jiang, Fengxue Xin
A solventogenic Clostridium sp. strain CT7 which could utilize glycerol directly to produce high yields of butanol was isolated. In the presence of crude glycerol, strain CT7 synthesized butanol through a unique butanol-ethanol (BE) fermentation pathway in which acetone and 1,3-propanediol (1,3-PDO) were not produced. The genome of strain CT7 which has a G + C content of 30.3% was estimated to be 5.99 Mb and contained 4319 putative Open Reading Frames (ORF). The putative annotated genes, which play major roles in BE production from crude glycerol, included glycerol dehydrogenase gene ( gdh ), acetoacetyl-CoA transferase gene ( ctfA / B ), and bifunctional alcohol and aldehyde dehydrogenase gene ( adhE )...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729086/high-level-expression-of-thermomyces-dupontii-thermophilic-lipase-in-pichia-pastoris-via-combined-strategies
#8
Jianrong Wang, Zongze Wu, Tianyu Zhang, Yonghua Wang, Bo Yang
In this study, a combined strategy was used to improve the production of Thermomyces dupontii lipase (TDL) in Pichia pastoris . First, the native gene of TDL was optimized based on the codon usage of P. pastoris , ligated to pPICZαA and transformed in P. pastoris X33. A recombinant strain designated X33-T23 with the highest activity (1020 U/mL in shake flasks) amongst 216 recombinant colonies was selected for further investigations. To further increase the production of TDL, nine different secretion helper factor genes were transformed in the recombinant strain, X33-T23...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729085/isolation-and-characterization-of-an-estrogen-degrading-pseudomonas-putida-strain-sjte-1
#9
Pingping Wang, Daning Zheng, Rubing Liang
In this report, Pseudomonas putida SJTE1 isolated from an enrichment culture of sludge was confirmed to degrade natural estrogens (17β-estradiol, estrone, estriol), estrogenic chemicals (naphthalene and phenanthrene) and testosterone. The strain completely degraded 1 mg/L 17β-estradiol in 24 h and transformed it into estrone; 90% and 75% of 50 mg/L and 100 mg/L 17β-estradiol were utilized in 7 days, respectively. The transformation efficiency of this strain against natural estrogens was much higher than that against other estrogenic chemicals...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729084/genetic-diversity-and-structure-of-dendrocalamus-hamiltonii-natural-metapopulation-a-commercially-important-bamboo-species-of-northeast-himalayas
#10
Rajendra K Meena, Maneesh S Bhandhari, Santan Barhwal, Harish S Ginwal
Dendrocalamus hamiltonii is a commercially important bamboo species of India, experiencing population depletion due to heavy extraction from natural forests. Nuclear simple sequence repeats (nSSRs) were used to study the genetic diversity and population genetic structure of 19 natural stands of D. hamiltonii distributed across the northeast Himalayas. A total of 68 nSSR primer pairs of D. latiflorus and Bambusa arundinacea have been tested in D. hamiltonii for their transferability, out of which 17 primers showing positive and polymorphic amplification were used for genotyping...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729083/the-healing-property-of-a-bioactive-wound-dressing-prepared-by-the-combination-of-bacterial-cellulose-bc-and-zingiber-officinale-root-aqueous-extract-in-rats
#11
Parastoo Pourali, Behrooz Yahyaei
Gluconacetobacter xylinus was used for production of bacterial cellulose (BC). The obtained BC was washed and floated in the nontoxic dose of the herb aqueous extract that was obtained from the methyl thiazol tetrazolium (MTT) assay. Twenty-four Wistar rats were divided into four separated groups and after inducing the wounds (15 mm in diameter), each group was treated with honey, BC, herb aqueous extract, and the combination of BC-herb aqueous extract. Each day the contraction percentages of the wound sites were measured...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729082/an-insight-into-the-anticancer-mechanism-of-tribulus-terrestris-extracts-on-human-breast-cancer-cells
#12
Apurva Patel, Anjali Soni, Nikhat J Siddiqi, Preeti Sharma
Tribulus terrestris (TT), a herb belonging to Zygophyllaceae family is widely used due to its medicinal properties. This study was undertaken to elucidate the anticancer mechanism of TT on MCF-7 breast cancer cells. Cytotoxic effect of the herb was assessed by 3-(4,5-diethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptotic potential was assessed through DNA fragmentation, TUNEL and caspase 3 activity assays. Expressions of genes regulating the apoptotic pathway were examined by RT-PCR and expression of proteins was analyzed by immunocytochemistry...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729081/gold-nanoparticle-surface-engineering-strategies-and-their-applications-in-biomedicine-and-diagnostics
#13
REVIEW
Kuldeep Mahato, Sahil Nagpal, Mahero Ayesha Shah, Ananya Srivastava, Pawan Kumar Maurya, Shounak Roy, Amit Jaiswal, Renu Singh, Pranjal Chandra
Gold nanoparticles (AuNPs) have found a wide range of biomedical and environmental monitoring applications (viz. drug delivery, diagnostics, biosensing, bio-imaging, theranostics, and hazardous chemical sensing) due to their excellent optoelectronic and enhanced physico-chemical properties. The modulation of these properties is done by functionalizing them with the synthesized AuNPs with polymers, surfactants, ligands, drugs, proteins, peptides, or oligonucleotides for attaining the target specificity, selectivity and sensitivity for their various applications in diagnostics, prognostics, and therapeutics...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729080/enzymatic-hydrolysis-of-tropical-weed-xylans-using-xylanase-from-aureobasidium-melanogenum-pbuap46-for-xylooligosaccharide-production
#14
Tanutcha Patipong, Pongtharin Lotrakul, Panuwat Padungros, Hunsa Punnapayak, Wichanee Bankeeree, Sehanat Prasongsuk
The maximum yield of xylanase from Aureobasidium melanogenum PBUAP46 was 5.19 ± 0.08 U ml-1 when cultured in a production medium containing 3.89% (w/v) rice straw and 0.75% (w/v) NaNO3 as carbon and nitrogen sources, respectively, for 72 h. This enzyme catalyzed well and was relatively stable at pH 7.0 and room temperature (28 ± 2 °C). The produced xylanase was used to hydrolyze xylans from four tropical weeds, whereupon it was found that the highest amounts of reducing sugars in the xylan hydrolysates of cogon grass ( Imperata cylindrical ), Napier grass ( Pennisetum purpureum ), and vetiver grass ( Vetiveria zizanioides ) were at 20...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729079/expression-analysis-of-the-polyphenol-oxidase-gene-in-response-to-signaling-molecules-herbivory-and-wounding-in-antisense-transgenic-tobacco-plants
#15
Ejaz Aziz, Riffat Batool, Wasim Akhtar, Shazia Rehman, Per L Gregersen, Tariq Mahmood
Key message: We provide evidence that the expression of the PPO gene was significantly reduced in response to wounding, MeJ and herbivory in transgenic tobacco under wound-inducible Os RGLP2 promoter in an anti-sense orientation. Abstract: Polyphenol oxidase (PPO) genes play an important role in plant defense mechanisms against biotic and abiotic stresses. In the present study, a 655 bp core sequence of the potato PPO gene was placed under the control of wound-inducible Os RGLP2 promoter in an anti-sense direction to evaluate its potential effects during biotic ( Trialeurodes vaporariorum 's infestation) and various abiotic (wounding, MeJ, ABA) stresses...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729078/in-vitro-fermentation-of-arabinoxylan-from-oat-avena-sativa-l-by-pekin-duck-intestinal-microbiota
#16
Dandan Tian, Xiaoqing Xu, Qing Peng, Zhiguo Wen, Yuwei Zhang, Chenyang Wei, Yu Qiao, Bo Shi
Arabinoxylan (AX) is abundant in cereal grains used as feed for ducks. However, the duck intestinal microbes responsible for the degradation of AX are not fully understood. In this study, oat AX was degraded and utilized by different duck intestinal microbiota in vitro. Changes in short-chain fatty acids (SCFAs), branch-chain fatty acids, and the pH resulted from a 72-h AX fermentation in intestinal samples were measured. The addition of AX increased the concentration of isobutyric acid and decreased the concentrations of SCFAs...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729077/phyto-anti-biofilm-elicitors-as-potential-inhibitors-of-helicobacter-pylori
#17
Ashwini Prasad, Aishwarya Tripurasundari Devi, M N Nagendra Prasad, Farhan Zameer, Govindaraju Shruthi, Chandan Shivamallu
Helicobacter pylori ( H. pylori ) infection is a global public health concern. Due to its high adaptability in various adverse environments (temperature, pH, adhesion, phenotypic forms), targeting the bacterium is quite challenging. Moreover, due to its high persistence, decreased patience compliance and emerging antibiotic resistance, researchers have been forced to search for novel candidates with lesser or no side effects. Hence, in the current study, phytobioactives have been screened for its anti-biofilm attributes against H...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729076/biodegradation-of-phenmedipham-by-novel-ochrobactrum-anthropi-nc-1
#18
Namadev K Pujar, Shruti Laad, H G Premakshi, Shridhar V Pattar, Manisha Mirjankar, Chandrappa M Kamanavalli
An Ochrobactrum anthropi bacterial strain named as NC-1, capable of utilizing phenmedipham (PMP) herbicide as the sole of carbon source and energy for growth was isolated from pesticide-contaminated soil sample by enrichment culture technique. The isolated bacterial strain was identified as Ochrobactrum anthropi NC-1 (MH 796134) based on its morphological, cultural, biochemical characteristics and analysis of 16S rRNA gene sequence. The strain NC-1 could degrade more than 98.5% of PMP (2 mM) within 168 h...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729075/an-efficient-eco-friendly-approach-for-recycling-of-newspaper-waste
#19
Avtar Singh, Amanjot Kaur, Ravi Dutt Yadav, Ritu Mahajan
In this study, a chemical reduction strategy was explored for deinking of newspaper waste using xylano-pectinolytic catalysts. A remarkable reduction of 40% in toxic chemicals consumption was obtained by introducing this enzymatic approach of deinking along with improved level of various physical and optical properties. Similarly, a reduction of 18.89% and 17.68% in BOD, COD values of effluent has also been noticed. This combined deinking methodology also resulted in a gain of 5.82% in breaking length, 6.45% in tear factor, 8...
February 2019: 3 Biotech
https://read.qxmd.com/read/30729074/different-performance-of-escherichia-coli-mutants-with-defects-in-the-phosphoenolpyruvate-carbohydrate-phosphotransferase-system-under-low-glucose-condition
#20
Hao Niu, Ruirui Li, Juan Gao, Xiangyu Fan, Qiang Li, Pengfei Gu
In Escherichia coli , the transport and phosphorylation of glucose is mainly accomplished by the phosphoenolpyruvate-dependent glucose-specific phosphotransferase system (PTSGlc ), which is, therefore, frequently selected as a target for engineering to increase the intracellular level of phosphoenolpyruvate. Here we characterized the effects of a low glucose concentration on the growth, glucose consumption, and acetate secretion of individual strains with a single PTSGlc mutation. We found that most mutants accumulated similar amounts of biomass, consumed glucose at lower rates, and secreted less acetate compared with the wild-type parental strain...
February 2019: 3 Biotech
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