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Clinical and Experimental Reproductive Medicine

Pallop Pongsuthirak, Teraporn Vutyavanich
Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro -matured human oocytes derived from pregnant and non-pregnant women. Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later...
December 2018: Clinical and Experimental Reproductive Medicine
Dayong Lee, Soo Jin Han, Seul Ki Kim, Byung Chul Jee
Objective: The purpose of this retrospective study was to evaluate the appropriateness of various follicle-stimulating hormone (FSH) starting doses in expected normal responders based on the nomogram developed by La Marca et al. Methods: A total of 117 first in vitro fertilization cycles performed from 2011 to 2017 were selected. All women were expected normal responders and used a recombinant FSH and flexible gonadotropin-releasing hormone antagonist protocol. The FSH starting dose was empirically determined (150, 225, or 300 IU)...
December 2018: Clinical and Experimental Reproductive Medicine
Ramadan Saleh, Hanan Assaf, Wafaa M Abd El Maged, Mohamed Elsuity, Mohamed Fawzy
Objective: To investigate the effects of in vitro myo-inositol (Myo-Ins) supplementation of cryopreserved human semen on the cryo-survival rate (CSR). Methods: Semen samples were obtained from 41 infertile men. Following routine semen analysis, each sample was divided into two equal aliquots (0.5 mL each). One aliquot was treated with 1 mg of Myo-Ins dissolved in 10 µL of sperm preparation medium. The second aliquot was treated with 10 µL of the same medium (control)...
December 2018: Clinical and Experimental Reproductive Medicine
Gianmartin Cito, Maria Elisabetta Coccia, Rita Picone, Gabriella Nesi, Andrea Cocci, Sara Dabizzi, Giulio Garaffa, Rossella Fucci, Patrizia Falcone, Francesco Bertocci, Raffaella Santi, Luciana Criscuoli, Sergio Serni, Marco Carini, Alessandro Natali
Objective: To assess whether the "testicular pool" could be used for histological analysis and whether it gave more accurate information than the standard testicular biopsy. Methods: Between January 2017 and March 2018, this single-center prospective study included 60 azoospermic men undergoing conventional bilateral testicular sperm extraction. Six samples were excised from each testicle and transferred to an embryologist. One additional biopsy was randomly taken from each testis for a histological analysis...
December 2018: Clinical and Experimental Reproductive Medicine
Bashir Ahmad, Vinoy K Shrivastava, Ramadan Saleh, Ralf Henkel, Ashok Agarwal
Objective: Zearalenone (ZEA) is a mycotoxin with potent estrogenic effects. Saffron is an herbal product that has antioxidant activities. The objective of this study was to investigate the protective role of saffron against reproductive toxicity induced by ZEA in female mice. Methods: Ninety 8-week-old female mice were randomly allocated into three treatment groups. The first group received an intraperitoneal injection of ZEA (2.5 mg/kg) on alternate days. The second group received ZEA (2...
December 2018: Clinical and Experimental Reproductive Medicine
Zahra Zandieh, Fatemehsadat Amjadi, Haghighat Vakilian, Khashayar Aflatoonian, Elham Amirchaghmaghi, Alireza Fazeli, Reza Aflatoonian
Objective: The fallopian tubes play a critical role in the early events of fertilization. The rapid innate immune defense is an important part of the fallopian tubes. Toll-like receptor 3 (TLR3), as a part of the innate immune system, plays an important role in detecting viral infections. In this basic and experimental study, the effect of sex hormones on the function of TLR3 in the OE-E6/E7 cell line was investigated. Methods: The functionality of TLR3 in this cell line was evaluated by cytokine measurements (interleukin [IL]-6 and IL-1b) and the effects of sex hormones on TLR3 were tested by an enzyme-linked immunosorbent assay kit...
December 2018: Clinical and Experimental Reproductive Medicine
Youn Jeong Lee, Kyong Wook Yi
Stem cells are undifferentiated cells capable of self-renewal and differentiation into various cell lineages. Stem cells are responsible for the development of organs and regeneration of damaged tissues. The highly regenerative nature of the human endometrium during reproductive age suggests that stem cells play a critical role in endometrial physiology. Bone marrow-derived cells migrate to the uterus and participate in the healing and restoration of functionally or structurally damaged endometrium. This review summarizes recent research into the potential therapeutic effects of bone marrow-derived stem cells in conditions involving endometrial impairment...
December 2018: Clinical and Experimental Reproductive Medicine
In Ae Cho, Yeon Jee Lee, Hee Jung Lee, In Young Choi, Jeong Kyu Shin, Soon Ae Lee, Jong Hak Lee, Won Jun Choi
Objective: The favored method of preserving fertility in young female cancer survivors is cryopreservation and autotransplantation of ovarian tissue. Reducing hypoxia until angiogenesis takes place is essential for the survival of transplanted ovarian tissue. The aim of this study was to investigate the role of angiopoietin-1 (Angpt-1), angiopoietin-2 (Angpt-2), and vascular endothelial growth factor (VEGF) in ovarian tissue grafts that were cryopreserved using two methods. Methods: Ovarian tissues harvested from ICR mice were divided into three groups: group I (control), no cryopreservation; group II, vitrification in EFS (ethylene-glycol, ficoll, and sucrose solution)-40; and group III, slow freezing in dimethyl sulfoxide...
September 2018: Clinical and Experimental Reproductive Medicine
Jae Jun Shin, Kyung Eui Park, Young Min Choi, Hye-Ok Kim, Dong-Hee Choi, Woo Sik Lee, Jung-Hyun Cho
Objective: To prospectively evaluate the efficacy and safety of a fixed early gonadotropin-releasing hormone (GnRH) antagonist protocol compared to a conventional midfollicular GnRH antagonist protocol and a long GnRH agonist protocol for in vitro fertilization (IVF) in patients with polycystic ovary syndrome (PCOS). Methods: Randomized patients in all three groups (early antagonist, n=14; conventional antagonist, n=11; long agonist, n=11) received 21 days of oral contraceptive pill treatment prior to stimulation...
September 2018: Clinical and Experimental Reproductive Medicine
Minh Tam Le, Thi Tam An Nguyen, Thi Thai Thanh Nguyen, Van Trung Nguyen, Dinh Duong Le, Vu Quoc Huy Nguyen, Ngoc Thanh Cao, Alar Aints, Andres Salumets
Objective: In frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET). Methods: Patients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole 40 µm in diameter was made in the drilling group...
September 2018: Clinical and Experimental Reproductive Medicine
Seok-Gi Kim, Youn-Young Kim, Ji-Young Park, Su-Jin Kwak, Chang-Seok Yoo, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee, Hum-Dai Park, Hee-Jun Chi
Objective: To determine whether fragment removal on in vitro fertilization (IVF) day 2 improved the subsequent development and pregnancy outcomes of fragmented embryos compared to similar-grade embryos without fragment removal. Methods: This study was a retrospective analysis involving 191 IVF cycles in which all embryos had over 10% fragmentation (grade 3 or 4) on day 2 of the IVF-embryo transfer cycle from March 2015 to December 2017. IVF cycles were divided into the fragment removal group (n=87) and the no fragment removal group (n=104) as a control cohort...
September 2018: Clinical and Experimental Reproductive Medicine
Sibel Sak, Hacer Uyanikoglu, Adnan Incebiyik, Hatice Incebiyik, Nese Gul Hilali, Tevfik Sabuncu, Erdal Sak
Objective: The aim of this study was to compare serum fetuin-A levels and oxidative stress markers, as indicators of insulin resistance, in women with polycystic ovary syndrome (PCOS) and in healthy controls. Methods: This prospective case-control study included 46 patients with PCOS and 48 age- and body mass index-matched control women. Levels of serum hormones, fetuin-A, and oxidative stress markers were measured in blood samples taken during the early follicular period from each participant...
September 2018: Clinical and Experimental Reproductive Medicine
Namfon Inna, Usanee Sanmee, Ubol Saeng-Anan, Waraporn Piromlertamorn, Teraporn Vutyavanich
Objective: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. Methods: Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group 1, n=300), a group that underwent Cryotop vitrification (group 2, n=300), and a group that underwent our in-house freezing method (group 3, n=300). Results: There were no significant differences between groups 2 and 3 in the immediate survival rate (96...
September 2018: Clinical and Experimental Reproductive Medicine
Gi Young Kim
In an age when a small quantity of sperm can lead to pregnancy through in vitro fertilization or intracytoplasmic sperm injection, selecting healthy sperm is important. Sperm DNA fragmentation (SDF) is known to be higher in infertile men. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) and the alkaline comet test are SDF tests that directly measure DNA damage and have shown closer correlations with assisted reproduction results than indirect tools such as the sperm chromatin structure assay or the sperm chromatic dispersion test...
September 2018: Clinical and Experimental Reproductive Medicine
Hacer Uyanikoglu, Nese Gul Hilali, Mesut Yardimciel, Ismail Koyuncu
Objective: Prompt diagnosis and management are essential for saving the adnexal organs from infarction in cases of ovarian torsion (OT). This study aimed to determine the diagnostic significance of signal peptide, complement C1r/C1s, Uegf, and Bmp1 (CUB), and epidermal growth factor-like domain-containing protein-1 (SCUBE-1) levels in cases of OT, an emergent ischemic condition, and the relationship of SCUBE-1 with oxidative stress parameters. Methods: This prospective study was conducted among 15 OT patients and 20 age- and gravidity-matched healthy women...
June 2018: Clinical and Experimental Reproductive Medicine
Vidyalakshmi Subramanian, Aishwarya Ravichandran, Nivethitha Thiagarajan, Matheswari Govindarajan, Silambuchelvi Dhandayuthapani, Sujatha Suresh
Objective: The aim of this study was to measure reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in the seminal fluid of the male partners in couples undergoing intrauterine insemination and to evaluate correlations between these values and their semen parameters. Methods: The study was conducted at Vamsam Fertility Center, Coimbatore, India and enrolled 110 male patients from whom semen samples were collected. ROS production was measured by a thiobarbituric acid reactive species assay, and TAC was measured by a 2,2-diphenyl-2-picrylhydrazyl free radical assay...
June 2018: Clinical and Experimental Reproductive Medicine
Hamid Ahmadi, Fardin Fathi, Ashraf Moeini, Fardin Amidi, Aligholi Sobhani
Objective: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. Methods: Mice (6-8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size...
June 2018: Clinical and Experimental Reproductive Medicine
Dmab Dissanayake, H Patel, P S Wijesinghe
Objective: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into male germ cells using all- trans retinoic acid and Sertoli cell-conditioned medium. Methods: Human WJ-MSCs were propagated by the explant culture method, and cells at the second passage were induced with differentiation medium containing all- trans retinoic acid for 2 weeks...
June 2018: Clinical and Experimental Reproductive Medicine
Ayman Shehata Dawood, Hesham Abdelaziz Salem
The purpose of this paper is to review the current clinical uses of platelet-rich plasma (PRP) in the field of gynecology. All relevant articles published from January 2000 to December 2017 were reviewed and analyzed. The articles on PRP in the field of gynecology were mainly case series, pilot studies, or case reports. PRP is currently considered a new therapeutic modality for some disorders that are refractory to conventional drugs.
June 2018: Clinical and Experimental Reproductive Medicine
Ahmed T Alahmar
It has been estimated that approximately 15% of reproductive-age couples suffer from infertility. Male factors contribute to almost half of infertility cases, and in many patients the underlying cause of oligoasthenoteratozoospermia is unknown. Accumulating evidence suggests that oxidative stress plays a role as a contributing factor to male infertility, and reactive oxygen species have been shown to impair sperm function and motility and to damage sperm membrane and DNA. Therefore, this review explored the evidence provided by studies published from 2002 to 2017 on the impact of oral antioxidants (vitamin C, vitamin E, L-carnitine, coenzyme Q10, zinc, selenium, and pentoxifylline) on seminal fluid parameters in men with idiopathic oligoasthenoteratozoospermia...
June 2018: Clinical and Experimental Reproductive Medicine
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