Nadine Yossa, Sonny Huang, Travis Canida, Rachel Binet, Dumitru Macarisin, Rebecca Bell, Sandra Tallent, Eric Brown, Thomas Hammack
Reference methods for microbiological safety assessments of cosmetics rely on culture methods that reveal colonies of live microorganisms on growth media. Rapid molecular technologies, such as qPCR, detects the presence of target DNA in samples from dead and viable cells. DNA intercalating dyes, such as propidium monoazide (PMAxx), are capable of restricting PCR amplification to viable microbial cells. Here we developed singleplex and multiplex real time (qPCR) assays for the detection of Bacillus cereus (B...
March 18, 2023: Scientific Reports