journal
https://read.qxmd.com/read/36858628/comparative-study-of-three-commercial-blood-1%C3%A2-3-%C3%AE-d-glucan-detection-reagents-for-invasive-mycosis
#21
JOURNAL ARTICLE
Yuki Miyajima, Kentaro Nagaoka, Yusuke Takegoshi, Makito Kaneda, Yushi Murai, Kou Kimoto, Takayuki Kawamura, Akitoshi Ueno, Hitoshi Kawasuji, Koyomi Kawago, Yasutaka Fukui, Ippei Sakamaki, Yoshihiro Yamamoto
BACKGROUND: β-D-glucan detection is a useful diagnostic indicator of invasive mycosis. However, the differences among the commercial β-D-glucan assays are unclear. Herein, we explored the diagnostic value of various β-D-glucan assay reagents. METHODS: This prospective observational study involved 175 eligible patients suspected to have fungal infections. For all participants, culture examinations were conducted with specimens obtained from the infected site (or blood culture), and β-D-glucan was measured using three commercial kits: Wako β-glucan test (Wako), Fungitech G-test MKII "Nissui" (MKII), and Fungitech G-test ES "Nissui" (ES)...
2023: Medical Mycology Journal
https://read.qxmd.com/read/35173102/examination-of-cyp51a-mediated-azole-resistance-in-aspergillus-lentulus-using-crispr-cas9-genome-editing
#22
JOURNAL ARTICLE
Madoka Tateno, Takashi Umeyama, Tatsuya Inukai, Shogo Takatsuka, Yasutaka Hoshino, Satoshi Yamagoe, Somay Yamagata Murayama, Keiko Ishino, Yoshitsugu Miyazaki
Aspergillus lentulus was first reported in 2005 as a cryptic species of Aspergillus fumigatus, and since then, its resistance to azole drugs and the high mortality rate of infected individuals have emerged as problems. Although it has been reported that P450 14-α sterol demethylase (Cyp51) is involved in azole resistance in A. lentulus, the specific resistance mechanism has not been elucidated. In this study, we successfully introduced the entire A. fumigatus cyp51A gene into the cyp51A locus in A. lentulus using the CRISPR/Cas9 genome-editing system...
February 16, 2022: Medical Mycology Journal
https://read.qxmd.com/read/36450569/diversity-and-distribution-of-aspergillus-fumigatus-and-its-related-species-in-izu-and-ogasawara-islands-japan
#23
JOURNAL ARTICLE
Dai Hirose, Kohei Watanabe, Ryo Hagiuda, Ryuri Tachikawa, Takashi Kamijo, Takashi Yaguchi, Mitsuru Hirota
The taxon Aspergillus section Fumigati comprises several causative agents of aspergillosis. Here, the distribution of Aspergillus sect. Fumigati in outdoor environments of Izu and Ogasawara Islands was investigated. Different strains were isolated from soil samples collected from 68 sites on 9 islands (Izu-oshima, Toshima, Shikinejima, Kozushima, Miyakejima, Hachijojima, Mukojima, Chichijima, and Hahajima), including different landscapes, and identified using morphological characteristics and calmodulin (CaM) sequences...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36450568/artificial-intelligence-in-the-diagnosis-of-invasive-mold-infection-development-of-an-automated-histologic-identification-system-to-distinguish-between-aspergillus-and-mucorales
#24
JOURNAL ARTICLE
Naobumi Tochigi, Sota Sadamoto, Shinji Oura, Yasuko Kurose, Yoshitsugu Miyazaki, Kazutoshi Shibuya
BACKGROUND: Histopathological identification is usually required since the sensitivity of fungal culture is not sufficient for accurate diagnosis. On the other hand, pathological diagnosis, especially of molds, often is not accurate, even when performed by an experienced pathologist. This is particularly true in the differentiation between mucormycosis and aspergillosis, which have different drugs of choice and medical management. The diseases can easily become severe in a short period of time in accordance with the severity of the underlying disease or predisposing factors...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36450567/a-case-of-oral-candidiasis-caused-by-candida-dubliniensis-during-treatment-of-humanized-monoclonal-antibody-to-interleukin-17a
#25
JOURNAL ARTICLE
Kiminobu Takeda, Taketoshi Futatsuya, Kazushi Anzawa, Takashi Mochizuki, Akira Shimizu, Kazunori Yamada
We report the case of a 77-year-old woman who had been diagnosed with psoriasis vulgaris at another hospital at age 33 and treated with various therapies since then. At 61 years old, she was diagnosed with psoriatic arthritis with phalangitis, swelling of both hands, and sacroiliac arthritis, and was treated with oral immunotherapy. At age 76, treatment with a humanized monoclonal antibody to interleukin-17A (ixekizumab) was started due to increased pain associated with her hand inflammation. After ten months of this treatment, she visited our hospital with the complaint of tongue pain and white fungi on the tongue surface...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36450566/microsporum-canis-infection-in-a-cat-breeder-family-and-an-investigation-of-their-breeding-cats
#26
JOURNAL ARTICLE
Mai Watanabe, Hitoshi Tsuchihashi, Takasuke Ogawa, Yumi Ogawa, Etsuko Komiyama, Yusuke Hirasawa, Masataro Hiruma, Rui Kano, Shigaku Ikeda
Two pediatric cases of Microsporum canis infection that occurred in a cat breeder family and the isolation of dermatophytes from their 166 breeding cats are reported. The patients were a 16-month-old girl and her 26-month-old sister who both had tinea capitis. Their family consisted of six members: the sisters, their great-grandmother, grandmother, grandfather, and mother. Except for the two sisters, the family had no history of skin lesions. The grandmother had been a cat breeder for 20 years. We tested the cats using the hairbrush technique, and 56 of the 158 cats (35%) tested were positive for M...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36450565/pamps-and-host-immune-response-in-cryptococcal-infection
#27
REVIEW
Ko Sato, Kazuyoshi Kawakami
Cryptococcus spp. are yeast-type opportunistic fungal pathogens with thick polysaccharide capsules that infect the lungs via airborne routes and frequently cause fatal meningoencephalitis. The cellular immune mechanism plays a central role in controlling cryptococcal infection and is critically regulated by Th1-Th2 immune balance. Pathogens that have invaded the host are recognized by innate immune cells, and appropriate immune responses are initiated. Pathogen-associated molecular patterns (PAMPs) are recognized by macrophages and dendritic cells via pattern recognition receptors (PRRs), which trigger the inflammatory responses as the first line of host defense...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36450564/taxonomy-of-pathogenic-yeasts-candida-cryptococcus-malassezia-and-trichosporon
#28
REVIEW
Masako Takashima, Takashi Sugita
This review describes the changes in yeast species names in the previous decade. Several yeast species have been reclassified to accommodate the "One fungus=One name" (1F=1N) principle of the Code. As the names of medically important yeasts have also been reviewed and revised, details of the genera Candida, Cryptococcus, Malassezia, and Trichosporon are described in Section 3, along with the history of name changes. Since the phylogenetic positions of Candida species in several clades have not been clarified, revision of this species has not been completed...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36450563/antifungal-stewardship-task-shifting-required-of-pharmacists
#29
REVIEW
Fumiya Ebihara, Takumi Maruyama, Ken Kikuchi, Toshimi Kimura, Yukihiro Hamada
Similar to antimicrobial stewardship, the concept of antifungal stewardship (AFS) has also received attention. AFS outcomes include reduced healthcare costs, avoidance of adverse events, and increased implementation of therapeutic drug monitoring (TDM). Several processes and outcome measures have recently been reported for implementing AFS and evaluating its effectiveness in healthcare institutions. This review focuses on our AFS efforts to standardize treatment using a template for pharmacist-led patient intervention for candidemia and to evaluate TDM dosage adequacy rates for voriconazole...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36047187/-malassezia-display-a-hyphae-like-spaghetti-and-meatballs-configuration-in-keratotic-plugs
#30
JOURNAL ARTICLE
Otomi Cho, Mizuki Unno, Keita Aoki, Masako Takashima, Takashi Sugita
Malassezia are lipophilic yeasts in the skin microbiome that abundantly colonize all parts of human skin except for the soles of the feet. Fungal microbiome analysis of keratotic plugs from the noses of 10 healthy individuals identified Malassezia restricta as the predominant species, followed by Malassezia globosa. Malassezia hyphae were observed in 5 of the 10 individuals. The hyphae were curved and thick-walled with spherical thick-walled and grouped blastoconidia, described as a "spaghetti-and-meatballs" configuration...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36047186/cryptococcus-neoformans-met5-gene-is-not-essential-for-virulence-in-the-silkworm-infection-model
#31
JOURNAL ARTICLE
Phuong-Thao Nguyen, Ngoc-Hung Nguyen, Ying-Qian Kang, Kiminori Shimizu
The involvement of the MET5 gene in virulence of Cryptococcus neoformans was examined using the silkworm Bombyx mori infection model. In the virulence assay, the met5Δ mutant showed virulence not significantly different from the wild-type strain, suggesting that the MET5 gene is not essential for full virulence of C. neoformans. The effect of silkworm hemolymph on the survival of the met5Δ mutant was also tested. The C. neoformans met5Δ strain incubated in the silkworm hemolymph for five days remained viable, suggesting that silkworm hemolymph supports survival of the met5Δ strain...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36047185/first-isolation-of-neoscytalidium-dimidiatum-from-human-dermatomycosis-in-japan
#32
JOURNAL ARTICLE
Taketoshi Futatsuya, Akiko Ogawa, Kazushi Anzawa, Takashi Mochizuki, Akira Shimizu
Neoscytalidium dimidiatum is a common fungus that causes non-dermatophyte dermatomycosis in tropical regions, but there have been no reports of infection with N. dimidiatum in Japan. Here, we report the first isolation of N. dimidiatum from human dermatomycosis in Japan. A 62-year-old healthy Japanese male had been treated with oral terbinafine for tinea pedis diagnosed from a microscopic examination in 2003 with a lesion that was intractable. In 2020, re-identification by sequencing the internal transcribed spacer regions and the D1/D2 domain of the large-subunit (LSU) ribosomal RNA gene revealed that the pathogen was N...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36047184/discovery-of-tolerance-to-itraconazole-in-japanese-isolates-of-aspergillus-section-nigri-aspergillus-tubingensis-and-aspergillus-welwitschiae-by-microscopic-observation
#33
JOURNAL ARTICLE
Yasunobu Matsumoto, Makoto Suzuki, Hiroyoshi Nihei, Satoru Matsumoto
Aspergillus section Nigri, a group of black Aspergillus, has several cryptic species that were recently discovered to be intrinsically resistant to azole antifungals. In this study, susceptibility testing of 35 clinical isolates of Aspergillus tubingensis and Aspergillus welwitschiae in Japan was carried out using microdilution method. Strains tolerant to itraconazole in A. tubingensis (14/17 strains) and A. welwitschiae (6/18 strains) were discovered with hyphal growth and conidial germination above the minimal inhibitory concentration by microscopic observation, while no resistant strain was observed macroscopically...
2022: Medical Mycology Journal
https://read.qxmd.com/read/36047183/invasive-mucormycosis-and-aspergillosis-coinfection-associated-with-post-covid-19-pneumonia-in-a-tertiary-care-hospital
#34
JOURNAL ARTICLE
Mousumi Paul, Jaseetha Sasidharan, Juhi Taneja, Kuhu Chatterjee, S Zafar Abbas, Veena Chowdhury, Asim Das
Acute invasive fungal rhinosinusitis is a rare infection primarily affecting patients with co-morbidities like immunosuppression and poorly controlled diabetes. Mucormycosis is increasingly being reported in patients with SARS-CoV-2 (COVID-19). However, reports of coinfection of aspergillosis and mucormycosis involving nose, paranasal sinuses, orbit, and brain are rare in literature. We aimed to evaluate the patient demographics, clinical presentation, and management of cases presenting with mixed infection...
2022: Medical Mycology Journal
https://read.qxmd.com/read/35650071/rapid-molecular-detection-of-antifungal-resistant-strains-of-malassezia-pachydermatis
#35
JOURNAL ARTICLE
Rui Kano, Nobuo Murayama
Azole resistance in Malassezia pachydermatis has been reported in isolates from canine skin worldwide. Decreased susceptibility of M. pachydermatis to azoles has been hypothesized to potentially result from mutations in the ERG11 gene, which encodes lanosterol 14α-demethylase. To sequence the mutation hotspots of ERG11 in the isolates, we prepared primers (MPERG11hot2S and MPERG11hot2R) based on the conserved sequences of M. pachydermatis ERG11. DNA samples from azole-resistant and -susceptible strains were amplified by PCR using the primer pair...
2022: Medical Mycology Journal
https://read.qxmd.com/read/35650070/-countermeasures-against-aspergillosis-in-captive-penguins
#36
JOURNAL ARTICLE
Takahito Toyotome, Naoya Matsumoto, Megumi Itoh, Kazutaka Yamada
Aspergillosis is a major fungal infection in humans and animals. Penguins (Order Spheniscidae) are particularly susceptible to aspergillosis, and aspergillosis in captive penguins is presently a major problem. We were faced with the challenge of combating aspergillosis in an aquarium. As a solution, we organized a multidisciplinary aspergillosis control team, including a medical and veterinary mycologist. Since Aspergillus, including Aspergillus fumigatus, is abundant in soil, we thought it necessary to minimize contact between captive penguins and soil to prevent aspergillosis...
2022: Medical Mycology Journal
https://read.qxmd.com/read/35650069/-effect-of-empagliflozin-on-candida-glabrata-adhesion-to-vaginal-epithelial-cells
#37
JOURNAL ARTICLE
Naomi Sugimoto, Yuka Yamagishi, Hiroshige Mikamo
A high incidence of genital infections, such as vulvovaginal candidiasis, has been reported in patients with diabetes treated with sodium-glucose co-transporter type 2 inhibitors. This is because Candida growth and virulence are enhanced in high glucose environments. Our previous study demonstrated that the adhesive interaction between Candida complement receptors and a ligand on vaginal epithelial cells (intracellular adhesion molecule-1: ICAM-1) is a factor for Candida albicans colonization, and the high ICAM-1 expression by vaginal epithelial cells exposed to high glucose conditions increases C...
2022: Medical Mycology Journal
https://read.qxmd.com/read/35650068/a-case-of-kerion-celsi-caused-by-trichophyton-tonsurans-a-plate-culture-of-which-showed-yellow-green-fluorescence-under-uva-light
#38
JOURNAL ARTICLE
Fumiyo Yasuda-Sekiguchi, Aki Kamata, Ryoko Hosokawa, Michiyoshi Kouno, Shinichi Takahashi, Takashi Yaguchi, Kazuhiro Aoyama, Tomotaka Sato
We herein report a case of kerion celsi of the scalp and tinea corporis due to Trichophyton tonsurans. A 17-year-old Japanese male high school student who practiced judo had alopecic patches with severe inflammation on the scalp. We performed a fungal culture and identified the causative fungus as T. tonsurans. A plate culture of T. tonsurans showed lemon-yellow colonies with yellow-green fluorescence under UVA light, which are typical findings for Microsporum canis. However, genetic analysis of the ribosomal RNA gene of the isolate facilitated differential diagnosis of T...
2022: Medical Mycology Journal
https://read.qxmd.com/read/35228482/kerion-celsi-caused-by-nannizzia-gypsea-in-a-two-year-old-child-who-had-been-hospitalized-since-birth
#39
JOURNAL ARTICLE
Yui Toyosawa, Utako Kimura, Mana Kurihara, Hiromitsu Noguchi, Tadahiko Matsumoto, Masataro Hiruma, Takeshi Nakazawa, Kenji Takamori, Yasushi Suga
Nannizzia gypsea, previously known as Microsporum gypseum, is a geophilic dermatophyte that infects humans from the soil. We isolated N. gypsea from a two-year-old girl with kerion celsi. Because of her serious medical condition, she was admitted to the pediatric ward immediately after birth. We struggled to identify the route of infection, and eventually identified her grandmother's handmade belt, which covered the endotracheal-tube-holding device, as the infection source. To prevent indirect transmission of pathogenic microorganisms from outside the hospital environment, our hospital prohibited the bringing of belongings from outside...
2022: Medical Mycology Journal
https://read.qxmd.com/read/35228481/phenotypic-characteristics-of-prototheca-species-occurring-in-humans-and-animals
#40
JOURNAL ARTICLE
Rui Kano, Kazuo Satoh, Takashi Yaguchi, Michiaki Masuda, Koichi Makimura, G Sybren de Hoog
The genus Prototheca consists of achlorophyllic algae that are ubiquitous in the environment and also occur in animal intestines; occasionally, infections in humans and animals are observed. In this study, we conducted tests of assimilative abilities and thermotolerance in comparison with morphological characteristics of six opportunistic species (Prototheca blaschkeae, Prototheca bovis, Prototheca ciferrii, Prototheca cutis, Prototheca miyajii, and Prototheca wickerhamii) along with Prototheca paracutis. Five of the seven species could be differentiated by physiological characteristics, but P...
2022: Medical Mycology Journal
journal
journal
43631
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.