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AMB Express

Laura E Navas, Fernando D Martínez, María E Taverna, Morgan M Fetherolf, Lindsay D Eltis, Verónica Nicolau, Diana Estenoz, Eleonora Campos, Graciela B Benintende, Marcelo F Berretta
Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain Thermus sp. 2.9 and investigate its capacity to delignify lignocellulosic biomass. The purified enzyme displayed a blue color typical of laccases, showed strict copper dependence and retained 80% of its activity after 16 h at 70 °C...
February 12, 2019: AMB Express
Hugo R Soares, Rute Castro, Hélio A Tomás, Manuel J T Carrondo, Paula M Alves, Ana S Coroadinha
Hepatitis C virus (HCV) infects 3% of world population being responsible for nearly half a million deaths annually urging the need for a prophylactic vaccine. Retrovirus like particles are commonly used scaffolds for antigens presentation being the core of diverse vaccine candidates. The immunogenicity of host proteins naturally incorporated in retrovirus was hypothesized to impact the performance of retrovirus based vaccines. In this work, the capacity of engineered retrovirus like particles devoided of host protein CD81 to display HCV envelope antigens was compared to non-engineered particles...
February 7, 2019: AMB Express
Charles-David Dubé, Serge R Guiot
Extracellular polymeric substances (EPS) play major roles in the efficacy of biofilms such as anaerobic granules, ranging from structural stability to more specific functions. The EPS of three granular anaerobic sludges of different origins were studied and compared. Particularly, the peptides from the protein fraction were identified by mass spectrometry. Desulfoglaeba and Treponema bacterial genera and Methanosaeta and Methanobacterium archaeal genera were prominent in all three sludges. Methanosaeta concilii proteins were the most represented in EPS of all three sludges studied...
February 7, 2019: AMB Express
Ruth Nahurira, Junhuan Wang, Yanchun Yan, Yang Jia, Shuanghu Fan, Ibatsam Khokhar, Adel Eltoukhy
Members of genus Gordonia are known to degrade various xenobitics and produce secondary metabolites. The genome of a halotorelant phthalic acid ester (PAEs) degrading actinobacterium Gordonia alkanivorans strain YC-RL2 was sequenced using Biosciences RS II platform and Single Molecular Real-Time (SMRT) technology. The reads were assembled de novo by hierarchical genome assembly process (HGAP) algorithm version 2. Genes were annotated by NCBI Prokaryotic Genome Annotation Pipeline. The generated genome sequence was 4,979,656 bp with an average G+C content of 67...
February 4, 2019: AMB Express
Olugbenga Oludayo Oluwasina, Ifunanya Vivian Ezenwosu, Clement Olusola Ogidi, Victor Olusegun Oyetayo
The need for a broad spectrum antimicrobial mouthwash is highly desirable to reduce, control and prevent various types of dental diseases. Hence, research into the production of herbal toothpaste to suppress the incidence of dental diseases is pertinent. The present study formulated herbal toothpastes from edible and medicinal plants namely; Syzygium aromaticum, Dennettia tripetala, and Jatropha curcas latex. The antimicrobial activity of the toothpastes was assessed against some oral pathogenic microorganisms using agar well diffusion...
February 4, 2019: AMB Express
Huaxian Zhao, Hua Gao, Kai Ji, Bing Yan, Quanwen Li, Shuming Mo, Minggang Zheng, Qian Ou, Bo Wu, Nan Li, Chengjian Jiang
3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) is a key rate-limiting enzyme in aromatic amino acid anabolism. A new Iβ -type DAHPS gene (aro1A) was identified in a metagenomic library from subtropical marine mangrove sediment. The gene encoded a polypeptide composed of 272 amino acids and had a maximum similarity of 52.4% to a known DAHPS at the amino acid level. Multiple sequence alignment, homologous modeling, and molecular docking showed that Aro1A had the typical (β/α)8 barrel-shaped catalytic structural domain of DAHPS...
February 4, 2019: AMB Express
Yiqin Deng, Changhong Cheng, Jiawei Xie, Songlin Liu, Hongling Ma, Juan Feng, Youlu Su, Zhixun Guo
Increasing evidence has revealed a close association between intestinal bacterial community and hosts health. However, it is unclear whether and what extend Baimang disease alters the intestinal microbiota in mud crab (Scylla paramamosain). Here, we conducted intestinal contents Illumina sequencing of healthy and Baimang diseased mud crab (S. paramamosain) to understand bacterial community variations among health status. In addition, bacterial functional predication was used to investigate whether and how the bacteria variations further change their functions? The phyla of Proteobacteria, Fusobacteria, Cyanobacteria, Tenericutes, Firmicutes, Bacteroidetes, and Spirochaetae constituted over 96...
February 2, 2019: AMB Express
Ángel Benito, Fernando Calderón, Santiago Benito
This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine. In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place. The sensorial properties of the final wines varied accordingly. Differences in mouthfeel properties and acidity occurred in the different fermentation trials...
February 2, 2019: AMB Express
Xiaofeng Jiang, Kun Qian, Guangping Liu, Laiyu Sun, Guoqing Zhou, Jingfen Li, Xinqiang Fang, Haixia Ge, Zhengbing Lv
The unique antimicrobial mechanism of antimicrobials make them a promising substitute for antibiotics for fighting drug-resistant bacteria. Both melittin and thanatin have antimicrobial bioactivity. However, thanatin does not inhibit the growth of Staphylococcus aureus. Melittin can inhibit S. aureus and has strong hemolytic activity. In the present study, the mutant fragments of melittin and thanatin were combined by flexible peptides to form a novel hybrid peptide, which was synthesized based on the secondary and tertiary structure prediction...
January 30, 2019: AMB Express
Jiyun Liu, Peifu Shi, Shahbaz Ahmad, Chunhua Yin, Xiaolu Liu, Yang Liu, Haiyang Zhang, Qianqian Xu, Hai Yan, Qingxiao Li
Co-culture of Bacillus coagulans and Candida utilis was firstly investigated in the efficient treatment of Lactobacillus fermentation wastewater (LFW) containing total organic carbon (TOC) of 22.0 g/L and total nitrogen (TN) of 2.4 g/L. The utilization of lactic acid by C. utilis was responsible for the relief of feedback inhibition to promote the growth of B. coagulans. The removal ratio of TOC by B. coagulans and C. utilis was only 9.1% and 22.7%, respectively, which was improved to 49.0% by co-culture...
January 30, 2019: AMB Express
Joosu Kuivanen, Alessandra Biz, Peter Richard
The most abundant hexuronate in plant biomass is D-galacturonate. D-Galacturonate is the main constituent of pectin. Pectin-rich biomass is abundantly available as sugar beet pulp or citrus processing waste and is currently mainly used as cattle feed. Other naturally occurring hexuronates are D-glucuronate, L-guluronate, D-mannuronate and L-iduronate. D-Glucuronate is a constituent of the plant cell wall polysaccharide glucuronoxylan and of the algal polysaccharide ulvan. Ulvan also contains L-iduronate. L-Guluronate and D-mannuronate are the monomers of alginate...
January 30, 2019: AMB Express
Yueqi Zhang, Hong Yang, Xinrui Yu, Haiyang Kong, Jiaming Chen, Huiying Luo, Yingguo Bai, Bin Yao
Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosaccharides. However, alkali extraction costs are very high, and the method also causes pollution. Therefore, this study focuses on elucidating a method to efficiently and directly degrade destarched wheat bran...
January 29, 2019: AMB Express
Ahmed Tawfike, Eman Zekry Attia, Samar Yehia Desoukey, Dina Hajjar, Arwa A Makki, Peter J Schupp, RuAngelie Edrada-Ebel, Usama Ramadan Abdelmohsen
Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR-ESI-MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR-ESI-MS data of the different fractions...
January 24, 2019: AMB Express
Dongxin Liu, Wencong He, Mingxia Jiang, Bing Zhao, Xichao Ou, Chunfa Liu, Hui Xia, Yang Zhou, Shengfen Wang, Yuanyuan Song, Yang Zheng, Qian Chen, Jiale Fan, Guangxue He, Yanlin Zhao
Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene...
January 23, 2019: AMB Express
Tao Pan, Rennv Wang, Kun Xiao, Wei Ye, Wei Dong, Meiying Xu
Extractive biodegradation of phenanthrene by Sphingomonas polyaromaticivorans was previously carried out in cloud point system (CPS). In this study, we explored the possibility of further increasing the efficiency of the culture by repeatedly reusing cells and the system for biodegradation. Three different recycling strategies were adopted. In reuse of cells plus CPS, cells were reused for 3 times while maintaining high degradation rates (> 90%). Thereafter, the accumulation of metabolites in the dilute phase resulted in a decrease in cell viability...
January 19, 2019: AMB Express
Derong Lin, Hongfu Cao, Yixin Zhong, Yichen Huang, Jinpeng Zou, Qi He, Ran Ji, Tao Qin, Yuan Chen, Dan Wang, Zhijun Wu, Wen Qin, Dingtao Wu, Hong Chen, Qing Zhang
Heavy metal lead, which enters the human body through food intake, endangers human health. Microbe has the ability of adsorbing heavy metal, among which lactic acid bacteria are promising microbes to adsorb and remove Pb2+ . The purpose of this study was to screen lactic acid bacteria from Ya'an pickle water to effectively remove Pb2+ . The 7 strains having strong ability to effectively remove Pb2+ were detected. These strains were identified by microscopic examination and 16S rDNA sequencing, 4 strains of Lactobacillus plantarum and 3 strains of Lactobacillus brevis were obtained...
January 19, 2019: AMB Express
Valentina Ambrogi, Francesca Bottacini, Joyce O'Sullivan, Mary O'Connell Motherway, Cao Linqiu, Barry Schoemaker, Margriet Schoterman, Douwe van Sinderen
Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize available carbon sources by means of secreted or intracellular glycosyl hydrolases (GHs). Among these, β-galactosidases are particularly relevant as they allow bifidobacteria to grow on β-galactosyl-linked saccharidic substrates, which are present in copious amounts in the milk-based diet of their infant host (e...
January 19, 2019: AMB Express
Ghadir S El-Housseiny, Khaled M Aboshanab, Mohammad M Aboulwafa, Nadia A Hassouna
Solid-state fermentation has a special advantage of preventing the foaming problem that obstructs submerged fermentation processes for rhamnolipid production. In the present work, a 50:50 mixture of sugarcane bagasse and sunflower seed meal was selected as the optimum substrate for rhamnolipid production using a Pseudomonas aeruginosa mutant 15GR and an impregnating solution including 5% v/v glycerol. Using Box-Behnken design, the optimum fermentation conditions were found to be an inoculum size 1% v/v, temperature 30 °C and unlike other studies, pH 8...
January 8, 2019: AMB Express
Abbas Tanhaeian, Mohammad Sadegh Damavandi, Davood Mansury, Kiarash Ghaznini
Due to the emergence of multidrug-resistant bacteria, treatment options for infectious diseases are decreasing. Bacteriocins are small antimicrobial peptides produced by numerous bacteria that offer alternative therapeutic strategies to combat multidrug-resistant bacterial infections. We evaluated the cloning, functional expression, and antimicrobial activities of enterocin P (EntP), a class II bacteriocin member, in Chinese hamster ovary (CHO) cells. A synthetic gene matching CHO cell codon usage was designed from the known mature amino acid sequence of EntP and cloned into the protein expression vector pcDNA™3...
January 7, 2019: AMB Express
Liang Huang, Ning Sun, Litong Ban, Yu Wang, Hongpeng Yang
Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liquid-and solid-state fermentation. Differences in the ability of various types of edible fungi to utilize biomass raw materials were analyzed by monitoring the fiber degradation rate during crop straw degradation, and changes in their cellulolytic enzyme systems during growth and metabolism were discussed...
January 7, 2019: AMB Express
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