journal
https://read.qxmd.com/read/37891640/comparison-of-digital-and-traditional-skin-wound-closure-assessment-methods-in-mice
#21
JOURNAL ARTICLE
Coco X Huang, Elisha Siwan, Sarah L Fox, Matilda Longfield, Stephen M Twigg, Danqing Min
BACKGROUND: Chronic skin wounds are a common complication of many diseases such as diabetes. Various traditional methods for assessing skin wound closure are used in animal studies, including wound tracing, calliper measurements and histological analysis. However, these methods have poorly defined wound closure or practical limitations. Digital image analysis of wounds is an increasingly popular, accessible alternative, but it is unclear whether digital assessment is consistent with traditional methods...
October 27, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37872597/regular-medical-checkup-program-in-k-medi-hub-to-enhance-the-welfare-of-laboratory-dogs-and-pigs
#22
JOURNAL ARTICLE
Gwang-Hoon Lee, Woori Jo, Joon-Suk Park, Tae-Ku Kang, Soo-Eun Sung, Taeho Oh, KilSoo Kim
BACKGROUND: The importance of animal welfare is being recognized worldwide. Recently, the increasing demand for enhanced laboratory animal welfare has led to clinically featured transformations of animal research institutes. This study aims to describe the process and findings of veterinary medical check-ups and its influence on laboratory dogs and pigs welfare. Regular medical checkups were conducted by the attending veterinarian twice a year to ensure the health and welfare of dogs and pigs in our animal research institute...
October 24, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37864254/chemosensitivity-to-doxorubicin-in-primary-cells-derived-from-tumor-of-fvb-n-trp53-tm1hw1-with-talen-mediated-trp53-mutant-gene
#23
JOURNAL ARTICLE
Woobin Yun, Ji Eun Kim, You Jeong Jin, Yu Jeong Roh, Hee Jin Song, Ayun Seol, Tae Ryeol Kim, Kyeong Seon Min, Eun Seo Park, Gi Ho Park, Hyun Gu Kang, Yeon Shik Choi, Dae Youn Hwang
BACKGROUND: To evaluate the chemosensitivity to doxorubicin (DOX) in two primary cells derived from a tumor of FVB/N-Trp53tm1Hw1 knockout (KO) mice with TALEN-mediated Trp53 mutant gene, we evaluated the cell survivability, cell cycle distribution, apoptotic cell numbers and apoptotic protein expression in solid tumor cells and ascetic tumor cells treated with DOX. RESULTS: The primary tumor cells showed a significant (P < 0.05) defect for UV-induced upregulation of the Trp53 protein, and consisted of different ratios of leukocytes, fibroblasts, epithelial cells and mesenchymal cells...
October 20, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37735416/ameliorative-effect-of-hesperidin-against-high-dose-sildenafil-induced-liver-and-testicular-oxidative-stress-and-altered-gene-expression-in-male-rats
#24
JOURNAL ARTICLE
Ibrahim M Ibrahim Laila, Samar HassabAllah Kassem, Marwa Salah ElDin Mohamed Diab
BACKGROUND: The clinical use of sildenafil citrate (Viagra), a drug used to treat erectile dysfunction, is limited because of its many side effects on tissues. In this context, we aimed to investigate the protective effects of hesperidin, a citrus flavonoid, on hepatic and testicular damage induced by a high dose of sildenafil citrate in male rats. Rats were randomly divided into four groups. The first group was used as the control group. The second group was orally administered sildenafil citrate at a high dose of 75 mg/kg thrice a week...
September 21, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37723570/exploration-of-the-anti-diabetic-potential-of-hydro-ethanolic-leaf-extract-of-koenigia-polystachya-l-an-edible-wild-plant-from-northeastern-india
#25
JOURNAL ARTICLE
Alokali Kiba, Dipankar Saha, Bhrigu Kumar Das
BACKGROUND: Globally, medicinal plants are used to treat diseases like diabetes. The present study evaluates the possible antioxidant, acute oral toxicity, the in-vitro and in-vivo antidiabetic potential of the hydro-ethanolic leaf extract of Koenigia polystachya (HELeKP) against beta-cell damage in experimentally induced diabetes mellitus. The DPPH (2,2-diphenyl-1-picrylhydrazine), ABTS [2,2'-azino bis-(3-ethylbenzothiazoline-6-sulfonic acid)], H2 O2 (Hydrogen peroxide), superoxide radical scavenging activity and NO (Nitric oxide) assay estimated the in-vitro antioxidant assay of HELeKP...
September 18, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37710339/the-mesocortical-dopaminergic-system-cannot-explain-hyperactivity-in-an-animal-model-of-attention-deficit-hyperactivity-disorder-adhd-spontaneously-hypertensive-rats-shr
#26
JOURNAL ARTICLE
Aysegul Gungor Aydin, Esat Adiguzel
BACKGROUND: Attention deficit hyperactivity disorder (ADHD) is one of the most prevalent neuropsychiatric disorders with morphological brain abnormalities. There is a growing body of evidence that abnormalities in the dopaminergic system may account for ADHD pathogenesis. However, it is not clear whether the dopaminergic system is hyper or hypoactive. To determine whether the DA neurons and/or axons deficiency might be the cause of the postulated dopaminergic hypofunction in spontaneously hypertensive rats (SHR, animal model of ADHD), this study examined the dopaminergic neurons and fibers in the brain tissues of SHRs and Wistar Kyoto rats (WKY, control animals)...
September 14, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37653550/trpc-expression-in-human-periodontal-ligament-cells-and-the-periodontal-tissue-of-periodontitis-mice-a-preliminary-study
#27
JOURNAL ARTICLE
Aeryun Kim, Ae Ri Kim, Yeong-Eui Jeon, Yun-Jung Yoo, Yu-Mi Yang, Eun-Jung Bak
BACKGROUND: Transient receptor potential canonical (TRPC) channels are non-selective cationic channels with permeability to Ca2+ and Na+ . Despite their importance, there are currently few studies on TRPC in the periodontal ligament (PDL) and bone cells in the dental field. To provide biological information regarding TRPC in PDL cells and periodontal tissue, we evaluated TRPC channels expression in the osteoblast differentiation of PDL cells and periodontitis-induced tissue. Human PDL cells were cultured in osteogenic differentiation media for 28 days, and the expression of Runx2, osteocalcin (OCN), and TRPC1, 3, 4, and 6 was evaluated by real-time PCR...
September 1, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37533118/staphylococcus-xylosus-and-staphylococcus-aureus-as-commensals-and-pathogens-on-murine-skin
#28
REVIEW
Michael Battaglia, Lee Ann Garrett-Sinha
Skin ulcers, skin dermatitis and skin infections are common phenomena in colonies of laboratory mice and are often found at increased prevalence in certain immunocompromised strains. While in many cases these skin conditions are mild, in other cases they can be severe and lead to animal morbidity. Furthermore, the presence of skin infections and ulcerations can complicate the interpretation of experimental protocols, including those examining immune cell activation. Bacterial species in the genus Staphylococcus are the most common pathogens recovered from skin lesions in mice...
August 2, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37507806/a-case-study-assessing-the-impact-of-mating-frequency-on-the-reproductive-performance-of-the-hawaiian-bobtail-squid-euprymna-scolopes
#29
JOURNAL ARTICLE
Andrew G Cecere, Rachel A Cook, Tim I Miyashiro
BACKGROUND: The symbiosis between the Hawaiian bobtail squid Euprymna scolopes and bacterium Vibrio fischeri serves as a model for investigating the molecular mechanisms that promote the initial formation of animal-bacterial symbioses. Research with this system frequently depends on freshly hatched E. scolopes, but the husbandry factors that promote hatchling production in a mariculture facility remain underreported. Here we report on the reproductive performance of E. scolopes in response to decreased mating frequency...
July 28, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37381051/segmentation-algorithm-can-be-used-for-detecting-hepatic-fibrosis-in-sd-rat
#30
JOURNAL ARTICLE
Ji-Hee Hwang, Minyoung Lim, Gyeongjin Han, Heejin Park, Yong-Bum Kim, Jinseok Park, Sang-Yeop Jun, Jaeku Lee, Jae-Woo Cho
BACKGROUND: Liver fibrosis is an early stage of liver cirrhosis. As a reversible lesion before cirrhosis, liver failure, and liver cancer, it has been a target for drug discovery. Many antifibrotic candidates have shown promising results in experimental animal models; however, due to adverse clinical reactions, most antifibrotic agents are still preclinical. Therefore, rodent models have been used to examine the histopathological differences between the control and treatment groups to evaluate the efficacy of anti-fibrotic agents in non-clinical research...
June 28, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37381025/datura-metel-stramonium-exacerbates-behavioral-deficits-medial-prefrontal-cortex-and-hippocampal-neurotoxicity-in-mice-via-redox-imbalance
#31
JOURNAL ARTICLE
Vincent Onoriode Igben, Wilson Josiah Iju, Omogbiya Adrian Itivere, John Chukwuma Oyem, Peter Sunday Akpulu, Efe Endurance Ahama
BACKGROUND: Datura metel (DM) stramonium is a medicinal plant often abused by Nigerians due to its psychostimulatory properties. Hallucinations, confusion, agitation, aggressiveness, anxiety, and restlessness are reported amongst DM users. Earlier studies suggest that DM induces neurotoxicity and affect brain physiology. However, the exact neurological effects of DM extract in the medial prefrontal cortex (mPFC) and hippocampal morphology have not been elucidated. In this study, we evaluated the hypothesis that oral exposure to DM extract exerts a neurotoxic effect by increasing oxidative stress in the mPFC and the hippocampus and induces behavioral deficits in mice...
June 28, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37308929/development-of-hpv16-mouse-and-dog-models-for-more-accurate-prediction-of-human-vaccine-efficacy
#32
JOURNAL ARTICLE
Emmanuelle Totain, Loïc Lindner, Nicolas Martin, Yolande Misseri, Alexandra Iché, Marie-Christine Birling, Tania Sorg, Yann Herault, Alain Bousquet-Melou, Pascale Bouillé, Christine Duthoit, Guillaume Pavlovic, Severine Boullier
BACKGROUND: Animal models are essential to understand the physiopathology of human diseases but also to evaluate new therapies. However, for several diseases there is no appropriate animal model, which complicates the development of effective therapies. HPV infections, responsible for carcinoma cancers, are among these. So far, the lack of relevant animal models has hampered the development of therapeutic vaccines. In this study, we used a candidate therapeutic vaccine named C216, similar to the ProCervix candidate therapeutic vaccine, to validate new mouse and dog HPV preclinical models...
June 12, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37296447/case-report-on-successful-treatment-for-brain-abscess-in-a-japanese-monkey-macaca-fuscata
#33
JOURNAL ARTICLE
Tohru Kimura
BACKGROUND: A brain abscess in human beings is a focal infection of the central nervous system frequently characterized by areas of localized cerebritis and central necrosis surrounded by a well vascularized capsule. A brain abscess, although sporadically reported, is relatively rare disease in domestic animals (horses, cattle, goats and alpacas), companion animals (dogs and cats) and laboratory nonhuman primates. Brain abscesses are life threatening disease that needs early and aggressive veterinary therapy...
June 9, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37271817/identification-of-proteins-regulated-by-chlorogenic-acid-in-an-ischemic-animal-model-a-proteomic-approach
#34
JOURNAL ARTICLE
Murad-Ali Shah, Ju-Bin Kang, Phil-Ok Koh
BACKGROUND: Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce ischemic brain injury and was maintained for 24 h. Chlorogenic acid (30 mg/kg) or vehicle was administrated into the peritoneal cavity 2 h after MCAO surgery. The cerebral cortical tissues were collected for further study and a proteomic approach was performed to identify the proteins changed by chlorogenic acid in the MCAO animals...
June 5, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37264475/effects-of-isoflurane-and-xylazine-on-inducing-cerebral-ischemia-by-the-model-of-middle-cerebral-artery-occlusion-in-mice
#35
JOURNAL ARTICLE
Jinyoung Won, Zeeshan Ahmad Khan, Yonggeun Hong
Preclinical ischemic stroke studies extensively utilize the intraluminal suture method of middle cerebral artery occlusion (MCAo). General anesthesia administration is an essential step for MCAo, but anesthetic agents can lead to adverse effects causing death and making a considerable impact on inducing cerebral ischemia. The purpose of this study was to comparatively assess the effect of isoflurane and xylazine on transient cerebral ischemia in a mouse model of MCAo. Twenty animals were randomly divided into four groups: sham group (no MCAo), control group (MCAo under isoflurane, no agent till reperfusion), isoflurane group (MCAo under isoflurane continued till reperfusion), xylazine group (MCAo under isoflurane, and administration of xylazine till reperfusion)...
June 1, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37259162/use-of-laboratory-animals-and-issues-regarding-the-procurement-of-animals-for-research-in-korea
#36
JOURNAL ARTICLE
Na Ahn, Jaehak Park, Sangho Roh
BACKGROUND: Laboratory animals remain critical to biomedical research, despite the increasing availability of alternative approaches. Indeed, scientists strive to reduce and refine and replace the use of laboratory animals, even in the face of public calls for ever-more stringent regulation for the protection and care of animals in research. This report outlines the current status and legal regulatory issues with regard to the procurement and use of animals for research in Korea. RESULTS: The number of animals used for education and research purposes was increased nationwide, from 2...
June 1, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37189184/effects-of-more-natural-housing-conditions-on-the-muscular-and-skeletal-characteristics-of-female-c57bl-6j-mice
#37
JOURNAL ARTICLE
Paul Mieske, Julia Scheinpflug, Timur Alexander Yorgan, Laura Brylka, Rupert Palme, Ute Hobbiesiefken, Juliane Preikschat, Lars Lewejohann, Kai Diederich
BACKGROUND: Enrichment of home cages in laboratory experiments offers clear advantages, but has been criticized in some respects. First, there is a lack of definition, which makes methodological uniformity difficult. Second, there is concern that the enrichment of home cages may increase the variance of results in experiments. Here, the influence of more natural housing conditions on physiological parameters of female C57BL/6J mice was investigated from an animal welfare point of view...
May 16, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37161442/sars-cov-2-omicron-variant-causes-brain-infection-with-lymphoid-depletion-in-a-mouse-covid-19-model
#38
JOURNAL ARTICLE
Na Yun Lee, Youn Woo Lee, Seung-Min Hong, Dain On, Gyeong Min Yoon, See-He An, Ki Taek Nam, Jun-Young Seo, Jeon-Soo Shin, Yang-Kyu Choi, Seung Hyun Oh, Jun-Won Yun, Ho Young Lee, Kang-Seuk Choi, Je Kyung Seong, Jun Won Park
BACKGROUND: The Omicron variant has become the most prevalent SARS-CoV-2 variant. Omicron is known to induce milder lesions compared to the original Wuhan strain. Fatal infection of the Wuhan strain into the brain has been well documented in COVID-19 mouse models and human COVID-19 cases, but apparent infections into the brain by Omicron have not been reported in human adult cases or animal models. In this study, we investigated whether Omicron could spread to the brain using K18-hACE2 mice susceptible to SARS-CoV-2 infection...
May 9, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37055870/antihypertensive-and-antihyperglycemic-effects-of-combinations-of-losartan-with-metformin-and-or-glibenclamide-in-desoxycorticosterone-acetate-and-streptozotocin-induced-hypertensive-diabetic-rats
#39
JOURNAL ARTICLE
Emuesiri Goodies Moke, Eric Kelly Inanemo Omogbai, SammyDavies Ehiosu Osagie-Eweka, Adaeze Phina Uchendu, Odion Martha Obayuwana, Elizabeth Okoro-Akpandu, Benneth Ben-Azu
BACKGROUND: Hypertension is a medical condition that often comorbidly exist in patients with type II diabetes. Therefore, it is very important to manage both conditions simultaneously to mitigate the complications and mortality connected with this comorbidity. Hence, this study investigated the antihypertensive and antihyperglycemic effects of combinations of losartan (LOS) with metformin (MET) and/or glibenclamide (GLB) in hypertensive diabetic rats. Hypertensive diabetic state was induced with desoxycorticosterone acetate (DOCA) and streptozotocin (STZ) in adult Wistar rats...
April 14, 2023: Laboratory Animal Research
https://read.qxmd.com/read/37055821/regenerative-medicine-owes-to-microsurgery
#40
JOURNAL ARTICLE
Kamran Shirbache, Hossein Nematian, Mohammad Hossein Nabian
New findings in regenerative medicine have always been combined with numerous animal studies. Therefore, choosing the right translational animal model plays an important role in transferring as much basic knowledge as possible to clinical application in this field. Since microsurgery has many capabilities to perform precise interventions on small animal models and facilitates other regenerative medicine procedures, based on scientific articles, we believe that the key to the flourishing of regenerative medicine in the clinic is the use of microsurgery...
April 13, 2023: Laboratory Animal Research
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