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Laboratory Animal Research

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https://read.qxmd.com/read/30671122/withdrawal-protective-effects-of-cultured-and-fermented-ginseng-extracts-against-scopolamine-induced-memory-loss-in-a-mouse-model
#1
Song-Hee Han, Sung-June Kim, Young Won Yun, Sang Yoon Nam, Hu-Jang Lee, Beom-Jun Lee
[This retracts the article on p. 37 in vol. 34, PMID: 29628975.].
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671121/comparison-of-scopolamine-induced-cognitive-impairment-responses-in-three-different-icr-stocks
#2
Woo Bin Yoon, Hyeon Jun Choi, Ji Eun Kim, Ji Won Park, Mi Ju Kang, Su Ji Bae, Young Ju Lee, You Sang Choi, Kil Soo Kim, Young-Suk Jung, Joon-Yong Cho, Dae Youn Hwang, Hyun Keun Song
Cognitive impairment responses are important research topics in the study of degenerative brain diseases as well as in understanding of human mental activities. To compare response to scopolamine (SPL)-induced cognitive impairment, we measured altered parameters for learning and memory ability, inflammatory response, oxidative stress, cholinergic dysfunction and neuronal cell damages, in Korl:ICR stock and two commercial breeder stocks (A:ICR and B:ICR) after relevant SPL exposure. In the water maze test, Korl:ICR showed no significant difference in SPL-induced learning and memory impairment compared to the two different ICRs, although escape latency was increased after SPL exposure...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671120/genetic-and-morphometric-characteristics-of-korean-wild-mice-kwm-hym-captured-at-chuncheon-south-korea
#3
Hajin Nam, Yoo Yeon Kim, Boyoung Kim, Won Kee Yoon, Hyoung-Chin Kim, Jun Gyo Suh
Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed to obtain data on the characteristics of a newly developing inbred strain (KWM/Hym) captured from Chuncheon, Korea. All of five Korean wild male mice have the zinc-finger Y ( ZfY ) gene. Also, all of 19 Korean wild mice used in this analysis have the AKV-type murine leukemia virus gene, indicating that Korean wild mice might be Mus musculus musculus ...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671119/crispr-cas9-mediated-knockout-of-cd47-causes-hemolytic-anemia-with-splenomegaly-in-c57bl-6-mice
#4
Joo-Il Kim, Jin-Sung Park, Jina Kwak, Hyun-Jin Lim, Soo-Kyung Ryu, Euna Kwon, Kang-Min Han, Ki-Taek Nam, Han-Woong Lee, Byeong-Cheol Kang
CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein α (SIRPα) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout ( CD47 -/- hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, CD47 -/- mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671118/nucleotide-binding-oligomerization-domain-1-is-dispensable-for-host-immune-responses-against-pulmonary-infection-of-acinetobacter-baumannii-in-mice
#5
Min-Jung Kang, Jin-A Choi, Joo-Hee Choi, Ah-Ra Jang, Ji-Yeon Park, Jae-Hun Ahn, Tae-Sung Lee, Dong-Yeon Kim, Jong-Hwan Park
Nucleotide-binding domain 1 (Nod1) is a cytosolic receptor that is responsible for the recognition of a bacterial peptidoglycan motif containing meso -diaminophimelic acid. In this study, we sought to identify the role of Nod1 in host defense in vivo against pulmonary infection by multidrug resistant Acinetobacter baumannii . Wildtype (WT) and Nod1-deficient mice were intranasally infected with 3×107 CFU of A. baumannii and sacrificed at 1 and 3 days post-infection (dpi). Bacterial CFUs, cytokines production, histopathology, and mouse β-defensins (mBD) in the lungs of infected mice were evaluated...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671117/inhibition-of-endoplasmic-reticulum-stress-in-high-fat-diet-induced-obese-c57bl-6-mice-efficacy-of-a-novel-extract-from-mulberry-morus-alba-leaves-fermented-with-cordyceps-militaris
#6
Mi Rim Lee, Su Ji Bae, Ji Eun Kim, Bo Ram Song, Jun Young Choi, Jin Ju Park, Ji Won Park, Mi Ju Kang, Hyeon Jun Choi, Young Whan Choi, Kyung Mi Kim, Dae Youn Hwang
A few clues about correlation between endoplasmic reticulum (ER) stress and mulberry ( Morus alba ) leaves were investigated in only the experimental autoimmune myocarditis and streptozotocin-induced diabetes. To investigate whether a novel extract of mulberry leaves fermented with Cordyceps militaris (EMfC) could suppress ER in fatty liver, alterations in the key parameters for ER stress response were measured in high fat diet (HFD)-induced obese C57L/6 mice treated with EMfC for 12 weeks. The area of adipocytes in the liver section were significantly decreased in the HFD+EMfC treated group as compared to the HFD+Vehicle treated group, while their level was higher in HFD+Vehicle treated group than No treated group...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671116/crispr-cas9-mediated-generation-of-a-plac8-knockout-mouse-model
#7
HyunJeong Lee, Joo-Il Kim, Jin-Sung Park, Jae-Il Roh, Jaehoon Lee, Byeong-Cheol Kang, Han-Woong Lee
Placenta specific 8 (PLAC8, also known as ONZIN) is a multi-functional protein that is highly expressed in the intestine, lung, spleen, and innate immune cells, and is involved in various diseases, including cancers, obesity, and innate immune deficiency. Here, we generated a Plac8 knockout mouse using the CRISPR/Cas9 system. The Cas9 mRNA and two single guide RNAs targeting a region near the translation start codon at Plac8 exon 2 were microinjected into mouse zygotes. This successfully eliminated the conventional translation start site, as confirmed by Sanger sequencing and PCR genotyping analysis...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671115/comparison-of-the-anesthetic-effects-of-2-2-2-tribromoethanol-on-icr-mice-derived-from-three-different-sources
#8
Mi Ree Lee, Hye Rin Suh, Myeong Whan Kim, Joon Young Cho, Hyun Keun Song, Young Suk Jung, Dae Youn Hwang, Kil Soo Kim
This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, Avertin®) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak CO2 (ETCO2 ), mean arterial blood pressure, heart rate, oxygen saturation (SpO2 ), body temperature, pH, PCO2 , and PO2 of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671114/generation-of-knockout-mouse-models-of-cyclin-dependent-kinase-inhibitors-by-engineered-nuclease-mediated-genome-editing
#9
Bo Min Park, Jae-Il Roh, Jaehoon Lee, Han-Woong Lee
Cell cycle dysfunction can cause severe diseases, including neurodegenerative disease and cancer. Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in various cancers. Mice lacking the tumor suppressors p16Ink4a ( Cdkn2a , cyclin-dependent kinase inhibitor 2a), p19Arf (an alternative reading frame product of Cdkn2a ,), and p27Kip1 ( Cdkn1b , cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671113/disruption-of-the-tff1-gene-in-mice-using-crispr-cas9-promotes-body-weight-reduction-and-gastric-tumorigenesis
#10
Hyejeong Kim, Haengdueng Jeong, Yejin Cho, Jaehoon Lee, Ki Taek Nam, Han-Woong Lee
Trefoil factor 1 ( TFF1 , also known as pS2) is strongly expressed in the gastrointestinal mucosa and plays a critical role in the differentiation of gastric glands. Since approximately 50% of all human gastric cancers are associated with decreased TFF1 expression, it is considered a tumor suppressor gene. TFF1 deficiency in mice results in histological changes in the antral and pyloric gastric mucosa, with severe hyperplasia and dysplasia of epithelial cells, resulting in the development of antropyloric adenoma...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671112/-18-f-fet-pet-is-a-useful-tool-for-treatment-evaluation-and-prognosis-prediction-of-anti-angiogenic-drug-in-an-orthotopic-glioblastoma-mouse-model
#11
Ok-Sun Kim, Jang Woo Park, Eun Sang Lee, Ran Ji Yoo, Won-Il Kim, Kyo Chul Lee, Jae Hoon Shim, Hye Kyung Chung
O-2-18 F-fluoroethyl-l-tyrosine ([18 F]FET) has been widely used for glioblastomas (GBM) in clinical practice, although evaluation of its applicability in non-clinical research is still lacking. The objective of this study was to examine the value of [18 F]FET for treatment evaluation and prognosis prediction of anti-angiogenic drug in an orthotopic mouse model of GBM. Human U87MG cells were implanted into nude mice and then bevacizumab, a representative anti-angiogenic drug, was administered. We monitored the effect of anti-angiogenic agents using multiple imaging modalities, including bioluminescence imaging (BLI), magnetic resonance imaging (MRI), and positron emission tomography-computed tomography (PET/CT)...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671111/-bacopa-monnieri-extract-improves-novel-object-recognition-cell-proliferation-neuroblast-differentiation-brain-derived-neurotrophic-factor-and-phosphorylation-of-camp-response-element-binding-protein-in-the-dentate-gyrus
#12
Hyun Jung Kwon, Hyo Young Jung, Kyu Ri Hahn, Woosuk Kim, Jong Whi Kim, Dae Young Yoo, Yeo Sung Yoon, In Koo Hwang, Dae Won Kim
Bacopa monnieri is a medicinal plant with a long history of use in Ayurveda, especially in the treatment of poor memory and cognitive deficits. In the present study, we hypothesized that Bacopa monnieri extract (BME) can improve memory via increased cell proliferation and neuroblast differentiation in the dentate gyrus. BME was administered to 7-week-old mice once a day for 4 weeks and a novel object recognition memory test was performed. Thereafter, the mice were euthanized followed by immunohistochemistry analysis for Ki67, doublecortin (DCX), and phosphorylated cAMP response element-binding protein (CREB), and western blot analysis of brain-derived neurotrophic factor (BDNF)...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671110/a-comparison-of-metabolomic-changes-in-type-1-diabetic-c57bl-6n-mice-originating-from-different-sources
#13
Seunghyun Lee, Jae-Hwan Kwak, Sou Hyun Kim, Jieun Yun, Joon-Yong Cho, Kilsoo Kim, Daeyeon Hwang, Young-Suk Jung
Animal models have been used to elucidate the pathophysiology of varying diseases and to provide insight into potential targets for therapeutic intervention. Although alternatives to animal testing have been proposed to help overcome potential drawbacks related to animal experiments and avoid ethical issues, their use remains vital for the testing of new drug candidates and to identify the most effective strategies for therapeutic intervention. Particularly, the study of metabolic diseases requires the use of animal models to monitor whole-body physiology...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671109/regulation-of-gastrointestinal-hormones-during-laxative-activity-of-gallotannin-enriched-extract-isolated-from-galla-rhois-in-loperamide-induced-constipation-of-sd-rats
#14
Ji Eun Kim, Mi Ju Kang, Jun Young Choi, Jin Ju Park, Mi Rim Lee, Bo Ram Song, Hye Ryeong Kim, Ji Won Park, Hyeon Jun Choi, Su Ji Bae, Dae Youn Hwang
Regulation of gastrointestinal hormones have been reported in animal models for constipation undergoing laxative therapy when administered herbal products. We undertook to investigate whether the laxative activity of gallotannin-enriched extracts isolated from Galla Rhois (GEGR) affects the regulation of gastrointestinal hormones, by examining the concentration of four hormones and the activation of their receptors in the loperamide (Lop)-induced constipation model. Stool parameters, including number, weight and water content, were significantly recovered in the Lop+GEGR treated group, relative to the Lop+vehicle treated group; however, food intake and water consumption were maintained at a constant level...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671108/-in-vitro-and-in-vivo-inhibition-of-helicobacter-pylori-by-lactobacilllus-paracasei-hp7
#15
Seong-Soo Hong, Hyun-A Lee, Joo Yun Kim, Ji-Woong Jeong, Jae-Jung Shim, Jung Lyoul Lee, Jae-Hun Sim, Yungho Chung, Okjin Kim
The efficacy of standard therapeutic strategies for Helicobacter pylori ( H. pylori ) infection is decreasing over time due to the emergence of drug-resistant strains. As an alternative, the present study investigated the capacity of Lactobacilllus paracasei ( L. paracasei ) HP7, isolated from kimchi, to inhibit H. pylori growth. The effects of L. paracasei HP7 on H. pylori adhesion and H. pylori -induced inflammation were examined in AGS human gastric adenocarcinoma epithelial cells and a mouse model of H...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671107/induction-of-superovulation-in-mature-mice-and-rats-using-serum-of-spayed-female-dogs
#16
M H Nooranizadeh, A Mogheiseh, M Kafi, M Sepehrimanesh, H Vaseghi
The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671106/repeated-restraint-stress-promotes-hippocampal-neuronal-cell-ciliogenesis-and-proliferation-in-mice
#17
Kyounghye Lee, Hyuk Wan Ko
Stress severely disturbs physiological and mental homeostasis which includes adult neurogenesis in hippocampus. Neurogenesis in hippocampus is a key feature to adapt to environmental changes and highly regulated by multiple cellular signaling pathways. The primary cilium is a cellular organelle, which acts as a signaling center during development and neurogenesis in adult mice. However, it is not clear how the primary cilia are involved in the process of restraint (RST) stress response. Using a mouse model, we examined the role of primary cilia in repeated and acute RST stress response...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671105/hyperglycemia-aggravates-decrease-in-alpha-synuclein-expression-in-a-middle-cerebral-artery-occlusion-model
#18
Ju-Bin Kang, Dong-Kyun Kim, Dong-Ju Park, Murad-Ali Shah, Myeong-Ok Kim, Eun-Jung Jung, Han-Shin Lee, Phil-Ok Koh
Hyperglycemia is one of the major risk factors for stroke. Hyperglycemia can lead to a more extensive infarct volume, aggravate neuronal damage after cerebral ischemia. α-Synuclein is especially abundant in neuronal tissue, where it underlies the etiopathology of several neurodegenerative diseases. This study investigated whether hyperglycemic conditions regulate the expression of α-synuclein in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury. Male Sprague-Dawley rats were treated with streptozotocin (40 mg/kg) via intraperitoneal injection to induce hyperglycemic conditions...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671104/growing-pigs-developed-different-types-of-diabetes-induced-by-streptozotocin-depending-on-their-transcription-factor-7-like-2-gene-polymorphisms
#19
Ching-Fu Tu, Chi-Yun Hsu, Meng-Hwan Lee, Bo-Hui Jiang, Shyh-Forng Guo, Chai-Ching Lin, Tien-Shuh Yang
The different polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene promote variances in diabetes susceptibility in humans. We investigated whether these genotypes also promote differences in diabetic susceptibility in commercial pigs. Growing pigs (Landrace, both sex, 50-60 kg) with the C/C (n=4) and T/T (n=5) TCF7L2 genotypes were identified and intravenously injected with streptozotocin (STZ, 40 mg/kg) twice in weekly intervals, then a high-energy diet was offered. Oral glucose tolerance tests, blood analyses and the homeostasis model assessment-insulin resistance (HOMA-IR) index calculations were performed...
December 2018: Laboratory Animal Research
https://read.qxmd.com/read/30671103/changes-of-myelin-basic-protein-in-the-hippocampus-of-an-animal-model-of-type-2-diabetes
#20
Sung Min Nam, Hyun Jung Kwon, Woosuk Kim, Jong Whi Kim, Kyu Ri Hahn, Hyo Young Jung, Dae Won Kim, Dae Young Yoo, Je Kyung Seong, In Koo Hwang, Yeo Sung Yoon
In this study, we observed chronological changes in the immunoreactivity and expression level of myelin basic protein (MBP), one of the most abundant proteins in the central nervous system, in the hippocampus of Zucker diabetic fatty (ZDF) rats and their control littermates (Zucker lean control; ZLC). In the ZLC group, body weight steadily increased with age; the body weight of the ZDF group, however, peaked at 30 weeks of age, and subsequently decreased. Based on the changes of body weight, animals were divided into the following six groups: early (12-week), middle (30-week), and chronic (52-week) diabetic groups and their controls...
December 2018: Laboratory Animal Research
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