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Journals Asian Pacific Journal of Tropi...

Asian Pacific Journal of Tropical Biomedicine

https://read.qxmd.com/read/25183282/reticulo-cutaneous-fistula-due-to-the-ingestion-of-a-long-metallic-rod-in-a-cow
#21
JOURNAL ARTICLE
Azari Omid, Ali Asghar Mozaffari
A five-year-old cow was referred to the veterinary faculty hospital for treatment of a swelling mass with a cutaneous fistula at the left lower part of the chest wall, between 7th till 9th intercostals space. Abdominal pain in palpation of the mass was observed with no abnormality in clinical symptoms. In surgical exploration, skin incision was carried out on the swelling mass and surprisingly a sinus tract with a sharp metallic rod (26 cm length) that continued to the reticulum lumen was identified. Because of unsuccessful attempting to remove of the foreign body, flank laparotomy and rumenotomy was performed...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183281/clinical-radiological-and-molecular-diagnosis-correlation-in-serum-samples-from-patients-with-osteoarticular-tuberculosis
#22
JOURNAL ARTICLE
Guadalupe García-Elorriaga, Olga Martínez-Elizondo, Guillermo Del Rey-Pineda, César González-Bonilla
OBJECTIVE: To assess the role of polymerase chain reaction (PCR) in serum samples, in the diagnosis of osteoarticular tuberculosis (OTB) in a setting where only clinical and imaging diagnoses determine the treatment. METHODS: A total of 44 consecutive serum specimens were collected from clinically suspected OTB patients, based on clinical and radiological [X-ray or magnetic resonance imaging/computed tomography] features. They were screened by in-house nested PCR...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183280/anti-diabetic-effects-of-caulerpa-lentillifera-stimulation-of-insulin-secretion-in-pancreatic-%C3%AE-cells-and-enhancement-of-glucose-uptake-in-adipocytes
#23
JOURNAL ARTICLE
Bhesh Raj Sharma, Dong Young Rhyu
OBJECTIVE: To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera). METHODS: The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and α-glucosidase enzyme was measured in a cell free system. Then, interleukin-1β and interferon-γ induced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183279/down-regulated-expression-of-npm1-in-ims-m2-cell-line-by-epigallocatechin-3-gallate
#24
JOURNAL ARTICLE
Hoang Thanh Chi, Bui Thi Kim Ly, Hoang Anh Vu, Yuko Sato, Phu Chi Dung, Phan Thi Xinh
OBJECTIVE: To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. METHODS: Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis. RESULTS: EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183278/knowledge-attitude-and-practices-related-to-cutaneous-leishmaniasis-in-an-endemic-focus-of-cutaneous-leishmaniasis-southern-iran
#25
JOURNAL ARTICLE
Bahador Sarkari, Asgari Qasem, Mohammad Reza Shafaf
OBJECTIVE: To assess knowledge, attitudes, and practices (KAP) of inhabitants of an endemic area in Fars province toward cutaneous leishmaniasis (CL). METHODS: The study was carried out in Lapui district in Fars province, south of Iran, one of the most important foci of CL in this province. Sample size (237 residents) was calculated based on population. House-to-house survey was done to collect the data regarding knowledge, attitudes, and practices of the inhabitants...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183277/an-initial-study-of-insect-succession-on-decomposing-rabbit-carrions-in-harare-zimbabwe
#26
JOURNAL ARTICLE
Nyasha Mabika, Ron Masendu, Gilbert Mawera
OBJECTIVE: To investigate insects visiting sun exposed and shaded decomposing rabbit carcasses and to establish the relationship between insects and carcasses which may be of forensic importance in Harare. METHODS: Two rabbits weighing 2.3 kg and 2.5 kg were killed by sharp blows on the head. One was exposed to the sun while the other was placed under shade. The carcasses were allowed to decompose and insects were collected twice a day for the first week and thereafter once a day up to the end of the 7 weeks...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183276/modified-technique-of-wolbachia-removal-from-malaysian-aedes-albopictus
#27
JOURNAL ARTICLE
Sylvia Joanne, Indra Vythilingam, Nava Yugavathy, Jonathan Inbaraj Doss
OBJECTIVE: To develop an artificial and modified Wolbachia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus (Ae. albopictus) so as to be able to produce generations of Wolbachia free offsprings. METHODS: In this study, seven different tetracycline treatment methods were conducted to obtain the best removal method. Four methods focused on larvae tetracycline treatment, one method on both larvae and adult tetracycline treatment and the last two methods on adult mosquito sucrose treatment...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183275/in-vitro-cytotoxicity-of-indonesian-stingless-bee-products-against-human-cancer-cell-lines
#28
JOURNAL ARTICLE
Paula M Kustiawan, Songchan Puthong, Enos T Arung, Chanpen Chanchao
OBJECTIVE: To screen crude extracts of propolis, bee pollen and honey from four stingless bee species [Trigona incisa (T. incisa)], Timia apicalis, Trigona fusco-balteata and Trigona fuscibasis) native to East Kalimantan, Indonesia for cytotoxic activity against five human cancer cell lines (HepG2, SW620, ChaGo-I, KATO-III and BT474). METHODS: All samples were extracted with methanol, and then subpartitioned with n-hexane and ethyl acetate. Each crude extract was screened at 20 µg/mL for in vitro cytotoxicity against the cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183274/cytotoxicity-screening-of-melastoma-malabathricum-extracts-on-human-breast-cancer-cell-lines-in-vitro
#29
JOURNAL ARTICLE
Nurfariza Ahmad Roslen, Nur Aizura Mat Alewi, Hadji Ahamada, Mohammad Syaiful Bahari Abdull Rasad
OBJECTIVE: To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro. METHODS: A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations (100 µg/mL, 50 µg/mL, 25 µg/mL, 12...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183273/antioxidant-potential-of-rumex-vesicarius-l-in-vitro-approach
#30
JOURNAL ARTICLE
Tajdar Husain Khan, Majid Ahmad Ganaie, Nasir Ali Siddiqui, Aftab Alam, Mohd Nazam Ansari
OBJECTIVE: To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L. (R. vesicarius). METHODS: In the present study, acetone, ethyl acetate, n-butanol, and methanol extracts of R. vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate, DNA sugar damage, scavenging of hydrogen peroxide, diphenylphosphine DPPH radical scavenging activity, total phenolic content, total flavonoids content and total proanthocyanidin...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183272/jeju-seaweeds-suppress-lipopolysaccharide-stimulated-proinflammatory-response-in-raw-264-7-murine-macrophages
#31
JOURNAL ARTICLE
Eun-Jin Yang, Ji-Young Moon, Sang Suk Kim, Kyong-Wol Yang, Wook Jae Lee, Nam Ho Lee, Chang-Gu Hyun
OBJECTIVE: To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW 264.7 cells under lipopolysaccharide (LPS) stimulation. METHODS: Ethyl acetate fractions were prepared from five different types of Jeju seaweeds, Dictyopteris divaricata (D. divaricata), Dictyopteris prolifera (D. prolifera), Prionitis cornea (P. cornea), Grateloupia lanceolata (G. lanceolata), and Grateloupia filicina (G. filicina). They were screened for inhibitory effects on proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6)...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183271/effects-of-lactic-acid-bacteria-isolated-from-fermented-mustard-on-lowering-cholesterol
#32
JOURNAL ARTICLE
Shu Chen Wang, Chen Kai Chang, Shu Chang Chan, Jiunn Shiuh Shieh, Chih Kwang Chiu, Pin-Der Duh
OBJECTIVE: To evaluate the ability of lactic acid bacteria (LAB) strains isolated from fermented mustard to lower the cholesterol in vitro. METHODS: The ability of 50 LAB strains isolated from fermented mustard on lowering cholesterol in vitro was determined by modified o-phtshalaldehyde method. The LAB isolates were analyzed for their resistance to acid and bile salt. Strains with lowering cholesterol activity, were determined adherence to Caco-2 cells. RESULTS: Strain B0007, B0006 and B0022 assimilated more cholesterol than BCRC10474 and BCRC 17010...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183270/the-presence-of-eucalyptol-in-artemisia-australis-validates-its-use-in-traditional-hawaiian-medicine
#33
JOURNAL ARTICLE
David Zant, Daniel A Gubler
OBJECTIVE: To identify the major organic compounds of Artemisia australis (A. australis), a plant used in traditional Hawaiian medicine for the treatment of asthma. METHODS: The dichloromethane extract of A. australis was analyzed by gas chromatography-mass spectroscopy and major compounds were identified by a National Institute of Standards and Technology library search and confirmed by peak enhancement. RESULTS: The major chemical components of A...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183269/proteomics-analysis-of-antimalarial-targets-of-garcinia-mangostana-linn
#34
JOURNAL ARTICLE
Wanna Chaijaroenkul, Artitiya Thiengsusuk, Kanchana Rungsihirunrat, Stephen Andrew Ward, Kesara Na-Bangchang
OBJECTIVE: To investigate possible protein targets for antimalarial activity of Garcinia mangostana Linn. (G. mangostana) (pericarp) in 3D7 Plasmodium falciparum clone using 2-dimensional electrophoresis and liquid chromatography mass-spectrometry (LC/MS/MS). METHODS: 3D7 Plasmodium falciparum was exposed to the crude ethanolic extract of G. mangostana Linn. (pericarp) at the concentrations of 12µg/mL (IC50 level: concentration that inhibits parasite growth by 50%) and 30 µg/mL (IC90 level: concentration that inhibits parasite growth by 90%) for 12 h...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25183268/antimicrobial-activity-against-periodontopathogenic-bacteria-antioxidant-and-cytotoxic-effects-of-various-extracts-from-endemic-thermopsis-turcica
#35
JOURNAL ARTICLE
Elif Burcu Bali, Leyla Açık, Gülçin Akca, Meral Sarper, Mualla Pınar Elçi, Ferit Avcu, Mecit Vural
OBJECTIVE: To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan, Vural & Küçüködük against periodontopathogenic bacteria, its antioxidant activity and cytotoxic effect on various cancer cell lines. METHODS: In vitro antimicrobial activities of ethanol, methanol, ethyl acetate (EtAc), n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion, minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)...
July 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25182953/a-retrospective-evaluation-of-the-quality-of-malaria-case-management-at-twelve-health-facilities-in-four-districts-in-zambia
#36
JOURNAL ARTICLE
Pascalina Chanda-Kapata, Emmanuel Chanda, Freddie Masaninga, Annette Habluetzel, Felix Masiye, Ibrahima Soce Fall
OBJECTIVE: To establish the appropriateness of malaria case management at health facility level in four districts in Zambia. METHODS: This study was a retrospective evaluation of the quality of malaria case management at health facilities in four districts conveniently sampled to represent both urban and rural settings in different epidemiological zones and health facility coverage. The review period was from January to December 2008. The sample included twelve lower level health facilities from four districts...
June 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25182952/study-of-the-efficacy-of-a-wheaton-coated-bottle-with-permethrin-and-deltamethrin-in-laboratory-conditions-and-a-who-impregnated-paper-with-bendiocarb-in-field-conditions
#37
JOURNAL ARTICLE
Nazaire Aïzoun, Roseric Azondekon, Rock Aïkpon, Virgile Gnanguenon, Razaki Osse, Alex Asidi, Martin Akogbéto
OBJECTIVE: To determine the efficacy of WHO impregnated paper and CDC coated bottle based on number of storage days and number of times of consecutive use, in the assessment of insecticide vector susceptibility tests in laboratory and field conditions. METHODS: Larvae and pupae of Anopheles gambiae s.l. mosquitoes were collected from the breeding sites in Seme-Kpodji and Cotonou districts in Southern Benin in April 2013 during the first rainy season. Anopheles gambiae s...
June 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25182951/pharmacognostical-study-and-establishment-of-quality-parameters-of-aerial-parts-of-costus-speciosus-a-well-known-tropical-folklore-medicine
#38
JOURNAL ARTICLE
Pradeep Singh, Ratan Lal Khosa, Shruti Srivastava, Garima Mishra, Keshri Kishor Jha, Sourabh Srivastava, Sangeeta, Ramesh Kumar Verma, Mohd Adil Tahseen
OBJECTIVE: To evaluate the diagnostic pharmacognostical characters of Costus speciosus (aerial parts) along with their physico-chemical parameters and fluorosence analysis. METHOD: The pharmacognostical characters were determined in terms of macroscopy, microscopy, powder microscopy, leaf constant, fluorescence analysis and preliminary phytochemical investigation. RESULTS: The findings of macroscopy revealed that leaves elliptic to oblong or oblong-lancoelate, thick, spirally arranged, with stem clasping sheaths up to 4 cm, flowers large, white, cone-like terminal spikes, with bright red bracts...
June 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25182950/hepatocurative-potential-of-vitex-doniana-root-bark-stem-bark-and-leaves-extracts-against-ccl4-induced-liver-damage-in-rats
#39
JOURNAL ARTICLE
James Dorcas Bolanle, Kadejo Olubukola Adetoro, Sallau Abdullahi Balarabe, Owolabi Olumuyiwa Adeyemi
OBJECTIVE: To evaluate the hepatocurative effects of aqueous root bark, stem bark and leaves of Vitex doniana in carbon tetrachloride (CCl4) induced liver damage and non induced liver damage albino rats. METHODS: A total of 60 albino rats (36 induced liver damage and 24 non induced liver damage) were assigned into liver damage and non liver damage groups of 6 rats in a group. The animals in the CCl4 induced liver damage groups, were induced by intraperitoneal injection with a single dose of CCl4 (1 mL/kg body weight) as a 1:1(v/v) solution in olive oil and were fasted for 36 h before the subsequent treatment with aqueous root bark, stem bark and leaves extracts of Vitex doniana and vitamin E as standard drug (100 mg/kg body weight per day) for 21 d, while the animals in the non induced groups were only treated with the daily oral administration of these extracts at the same dose...
June 2014: Asian Pacific Journal of Tropical Biomedicine
https://read.qxmd.com/read/25182949/in-vitro-%C3%AE-amylase-inhibitory-activity-and-in-vivo-hypoglycemic-effect-of-methanol-extract-of-citrus-macroptera-montr-fruit
#40
JOURNAL ARTICLE
Nizam Uddin, Md Rakib Hasan, Md Monir Hossain, Arjyabrata Sarker, A H M Nazmul Hasan, A F M Mahmudul Islam, Mohd Motaher H Chowdhury, Md Sohel Rana
OBJECTIVE: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). METHODS: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats...
June 2014: Asian Pacific Journal of Tropical Biomedicine
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