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Asian Pacific Journal of Tropical Biomedicine

T Kaviarasan, Sankar R Siva, A Yogamoorthi
[This retracts the article DOI: 10.1016/S2221-1691(12)60253-6.][This retracts the article DOI: 10.1016/S2221-1691(12)60253-6.].
February 2015: Asian Pacific Journal of Tropical Biomedicine
Fahmi Yousef Khan, Mohammed Abukhattab, Mohammed AbuKamar, Deshmukh Anand
OBJECTIVE: To describe the clinical presentation, underlying diseases, antimicrobial susceptibility, treatment and outcome of Klebsiella pneumoniae meningitis patients. METHODS: This retrospective study involved all patients with 15 years of age or older who admitted to Hamad General Hospital with culture proven Klebsiella pneumoniae meningitis from January 1, 2007 to December 31, 2012. RESULTS: A total of ten cases were identified (nine males and one female)...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Kanokwan Kulprachakarn, Nittaya Chansiw, Kanjana Pangjit, Chada Phisalaphong, Suthat Fucharoen, Robert C Hider, Sineenart Santitherakul, Somdet Srichairatanakool
OBJECTIVE: To evaluate the iron-chelating properties and free-radical scavenging activities of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) treatment in chronic iron-loaded β-thalassemic (BKO) mice. METHODS: The BKO mice were fed with a ferrocene-rich diet and were orally administered with CM1 [50 mg/(] for 6 months. Blood levels of non-transferrin bound iron, labile plasma iron, ferritin (Ft) and malondialdehyde were determined. RESULTS: The BKO mice were fed with an iron diet for 8 months which resulted in iron overload...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Bamba Sanata, Ouédraogo Abdoul Salam, Sangaré Ibrahim, Zida Adama, Cissé Mamoudou, Karou D Simplice, Simpore Jacques, Guiguemdé T Robert, Hennequin Christophe
OBJECTIVE: To identify Candida species in asymptomatic subjects in Bobo-Dioulasso (Burkina Faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry. METHODS: A cross-sectional study was conducted from January to February 2013 in Bobo-Dioulasso to collect fecal and urine specimens from voluntary donors. Fungal strains were isolated on Sabouraud dextrose agar and analyzed using matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Abdoul Karim Ouattara, Cyrille Bisseye, Bapio Valery Jean Télesphore Elvira Bazie, Birama Diarra, Tegwindé Rebeca Compaore, Florencia Djigma, Virginio Pietra, Remy Moret, Jacques Simpore
OBJECTIVE: To investigate 4 combinations of mutations responsible for glucose-6-phosphate dehydrogenase (G6PD) deficiency in a rural community of Burkina Faso, a malaria endemic country. METHODS: Two hundred individuals in a rural community were genotyped for the mutations A376G, G202A, A542T, G680T and T968C using TaqMan single nucleotide polymorphism assays and polymerase chain reaction followed by restriction fragment length polymorphism. RESULTS: The prevalence of the G6PD deficiency was 9...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Shaheen Faizi, Saima Sumbul, Muhammed Ali Versiani, Rubeena Saleem, Aisha Sana, Hira Siddiqui
OBJECTIVE: To explore the phytochemical constituents from petroleum ether and dichloromethane extracts of Moringa oleifera (M. oleifera) roots using GC/GC-MS. METHODS: A total of 5.11 kg fresh and undried crushed root of M. oleifera were cut into small pieces and extracted with petroleum ether and dichloromethane (20 L each) at room temperature for 2 d. The concentrated extracts were subjected to their GC-MS analysis. RESULTS: The GC-MS analysis of the petroleum ether and dichloromethane extracts of M...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Yousef Emamipoor, Mahmood Maziah
OBJECTIVE: To develop a protocol for breaking of seed dormancy and increasing the seed germination rate of Bunium persicum. METHODS: The seeds were treated with 3.1, 6.3, 12.5, 25, 50 and 100 µmol/L of benzyl aminopurine, gibberellic acid (GA3), thidiazuron (TDZ) and forchlorfenuron. Then, seeds were transferred to two different temperature conditions including room temperature (25 °C) and chilling temperature (2-5 °C). RESULTS: The treatment of moist seeds with chilling temperature (2-5 °C) broke seed dormancy and showed maximum germination, which was 54...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Hira Fahim, Javid Iqbal Dasti, Ihsan Ali, Safia Ahmed, Muhammad Nadeem
OBJECTIVE: To evaluate physico-chemical properties and antimicrobial potential of indigenous honey samples against different reference strains including Escherichia coli ATCC 8739, Enterobacter aerogenes ATCC 13048, Pseudomonas aeroginosa ATCC 9027, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Salmonella typhi ATCC 14028, Klebsiella pneumonia ATCC 13883, Aspergillus niger ATCC 16404, Rhizopus oligosporus PCSIR1, Candida albicans ATCC 14053 and Candida utilis ATCC 9950...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Carolina Santiago, Lin Sun, Murray Herbert Gibson Munro, Jacinta Santhanam
OBJECTIVE: To study bioactivity and compounds produced by an endophytic Phoma sp. fungus isolated from the medicinal plant Cinnamomum mollissimum. METHODS: Compounds produced by the fungus were extracted from fungal broth culture with ethyl acetate. This was followed by bioactivity profiling of the crude extract fractions obtained via high performance liquid chromatography. The fractions were tested for cytotoxicity to P388 murine leukemic cells and antimicrobial activity against bacteria and pathogenic fungi...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Sahar Y Al-Okbi, Doha A Mohamed, Thanaa E Hamed, Reham Sh Esmail, Souria M Donya
OBJECTIVE: To investigate the protective effect of extracts prepared from avocado, walnut, flaxseed and Eruca sativa seeds in a rat model of kidney dysfunction induced by intraperitoneal cisplatin. METHODS: Ethanol and petroleum ether extracts mixture was prepared from each plant. Six groups of rats were conducted; control healthy, cisplatin group and four test groups where rats were given daily oral dose of each extract mixture before cisplatin injection. Different biochemical and cytogenetic parameters and kidney histopathology were determined...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Ridzwan Bin Hashim, Nurul Alia Azizan, Zaitunnatakin Zamli, Farah Hanis Zulkipli, Nurzafirah Mazlan, Osama Yousef Althunibat
OBJECTIVE: To determine lethal median dose (LD50) and histopathological toxicity of water extract of Holothuria atra (H. atra) in mice. METHODS: The behavioral changes, mortality and histopathology examination on liver were assessed in mice 14 d after the administration (i.p.) of H. atra water extract. Seven doses (10, 20, 30, 50, 100, 150 and 200 mg/kg) of H. atra were used. The control group was treated with normal saline. RESULTS: In the acute study in mice, the water extracts of H...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Sidgi Syed Anwer Hasson, Mohammed Saeed Al-Balushi, Muzna Hamed Al Yahmadi, Juma Zaid Al-Busaidi, Elias Antony Said, Mohammed Shafeeq Othman, Talal Abdullah Sallam, Mohammed Ahmad Idris, Ali Abdullah Al-Jabri
OBJECTIVE: To investigate the levels of zinc-α-2-glycoprotein (ZAG) among Omani AIDS patients receiving combined antiretroviral therapy (cART). METHODS: A total of 80 Omani AIDS patients (45 males and 35 females), average age of 36 years, who were receiving cART at the Sultan Qaboos University Hospital (SQUH), Muscat, Oman, were tested for the levels of ZAG. In addition, 80 healthy blood donors (46 males and 34 females), average age of 26 years, attending the SQUH Blood Bank, were tested in parallel as a control group...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Hakan Söyüt, Elif Duygu Kaya, Sükrü Beydemir
OBJECTIVE: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). METHODS: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. RESULTS: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Kanungnit Congpuong, Rungniran Sukaram, Yuparat Prompan, Aibteesam Dornae
OBJECTIVE: To study the genetic diversity at the msp-1, msp-2, and glurp genes of Plasmodium falciparum (P. falciparum) isolates from 3 endemic areas in Thailand: Tak, Kanchanaburi and Ranong provinces. METHODS: A total of 144 P. falciparum isolates collected prior to treatment during January, 2012 to June, 2013 were genotyped. DNA was extracted; allele frequency and diversity of msp-1, msp-2, and glurp genes were investigated by nested polymerase chain reaction...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Beuy Joob, Viroj Wiwanitkit
No abstract text is available yet for this article.
August 2014: Asian Pacific Journal of Tropical Biomedicine
Morteza Izadi, Arman Is'haqi, Mohammad Ali Is'haqi, Nematollah Jonaidi Jafari, Fatemeh Rahamaty, Abdolali Banki
Nervous system infections are among the most important diseases in travellers. Healthy travellers might be exposed to infectious agents of central nervous system, which may require in-patient care. Progressive course is not uncommon in this family of disorders and requires swift diagnosis. An overview of the available evidence in the field is, therefore, urgent to pave the way to increase the awareness of travel-medicine practitioners and highlights dark areas for future research. In November 2013, data were collected from PubMed, Scopus, and Web of Knowledge (1980 to 2013) including books, reviews, and peer-reviewed literature...
August 2014: Asian Pacific Journal of Tropical Biomedicine
Azari Omid, Ali Asghar Mozaffari
A five-year-old cow was referred to the veterinary faculty hospital for treatment of a swelling mass with a cutaneous fistula at the left lower part of the chest wall, between 7th till 9th intercostals space. Abdominal pain in palpation of the mass was observed with no abnormality in clinical symptoms. In surgical exploration, skin incision was carried out on the swelling mass and surprisingly a sinus tract with a sharp metallic rod (26 cm length) that continued to the reticulum lumen was identified. Because of unsuccessful attempting to remove of the foreign body, flank laparotomy and rumenotomy was performed...
July 2014: Asian Pacific Journal of Tropical Biomedicine
Guadalupe García-Elorriaga, Olga Martínez-Elizondo, Guillermo Del Rey-Pineda, César González-Bonilla
OBJECTIVE: To assess the role of polymerase chain reaction (PCR) in serum samples, in the diagnosis of osteoarticular tuberculosis (OTB) in a setting where only clinical and imaging diagnoses determine the treatment. METHODS: A total of 44 consecutive serum specimens were collected from clinically suspected OTB patients, based on clinical and radiological [X-ray or magnetic resonance imaging/computed tomography] features. They were screened by in-house nested PCR...
July 2014: Asian Pacific Journal of Tropical Biomedicine
Bhesh Raj Sharma, Dong Young Rhyu
OBJECTIVE: To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera). METHODS: The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and α-glucosidase enzyme was measured in a cell free system. Then, interleukin-1β and interferon-γ induced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes...
July 2014: Asian Pacific Journal of Tropical Biomedicine
Hoang Thanh Chi, Bui Thi Kim Ly, Hoang Anh Vu, Yuko Sato, Phu Chi Dung, Phan Thi Xinh
OBJECTIVE: To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. METHODS: Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis. RESULTS: EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations...
July 2014: Asian Pacific Journal of Tropical Biomedicine
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