journal
https://read.qxmd.com/read/38505603/intravital-imaging-of-splenic-classical-monocytes-modifying-the-hepatic-cx3cr1-cells-motility-to-exacerbate-liver-fibrosis-via-spleen-liver-axis
#21
JOURNAL ARTICLE
Chenlu Han, Yujie Zhai, Yuke Wang, Xuwen Peng, Xian Zhang, Bolei Dai, Yuehong Leng, Zhihong Zhang, Shuhong Qi
UNLABELLED: CX3CR1+ cells play a crucial role in liver fibrosis progression. However, changes in the migratory behavior and spatial distribution of spleen-derived and hepatic CX3CR1+ cells in the fibrotic liver as well as their influence on the liver fibrosis remain unclear. METHODS: The CX3CR1GFP/+ transgenic mice and CX3CR1-KikGR transgenic mice were used to establish the CCl4-induced liver fibrosis model. Splenectomy, adoptive transfusion of splenocytes, in vivo photoconversion of splenic CX3CR1+ cells and intravital imaging were performed to study the spatial distribution, migration and movement behavior, and regulatory function of CX3CR1+ cells in liver fibrosis...
2024: Theranostics
https://read.qxmd.com/read/38505602/fsh-induces-emt-in-ovarian-cancer-via-alkbh5-regulated-snail-m6a-demethylation
#22
JOURNAL ARTICLE
Xingyan Xu, Xuefen Zhuang, Haowei Yu, Ping Li, Xiaosa Li, Huiping Lin, Jian-Peng Teoh, Yiwen Chen, Yuanlan Yang, Yang Cheng, Weiyu Chen, Xiaodong Fu
Background: The therapeutic benefits of targeting follicle-stimulating hormone (FSH) receptor in treatment of ovarian cancer are significant, whereas the role of FSH in ovarian cancer progresses and the underlying mechanism remains to be developed. Methods: Tissue microarray of human ovarian cancer, tumor xenograft mouse model, and in vitro cell culture were used to investigate the role of FSH in ovarian carcinogenesis. siRNA, lentivirus and inhibitors were used to trigger the inactivation of genes, and plasmids were used to increase transcription of genes...
2024: Theranostics
https://read.qxmd.com/read/38505601/multifunctional-sr-se-co-doped-zif-8-nanozyme-for-chemo-chemodynamic-synergistic-tumor-therapy-via-apoptosis-and-ferroptosis
#23
JOURNAL ARTICLE
Aimin Wu, Ming Han, Zihan Ni, Haoran Li, Yinyin Chen, Zhouping Yang, Yumei Feng, Zufeng He, Hua Zhen, Xianxiang Wang
Rationale: Cancer continues to be a significant public health issue. Traditional treatments such as surgery, radiotherapy, and chemotherapy often fall short because of intrinsic issues such as lack of specificity and poor drug delivery, leading to insufficient drug concentration at the tumor site and/or potential side effects. Consequently, improving the delivery of conventional chemotherapy drugs like doxorubicin (DOX) is crucial for their therapeutic efficacy. Successful cancer treatment is achieved when regulated cell death (RCD) of cancer cells, which includes apoptotic and non-apoptotic processes such as ferroptosis, is fundamental to successful cancer treatment...
2024: Theranostics
https://read.qxmd.com/read/38505600/g-protein-subunit-alpha-i2-s-pivotal-role-in-angiogenesis
#24
JOURNAL ARTICLE
Chao-Wen Bai, Lu Lu, Jia-Nan Zhang, Chengyu Zhou, Yi-Chao Ni, Ke-Ran Li, Jin Yao, Xiao-Zhong Zhou, Chang-Gong Lan, Cong Cao
Here we explored the potential role of Gαi2 (G protein subunit alpha i2) in endothelial cell function and angiogenesis. Methods: Genetic methodologies such as shRNA, CRISPR/Cas9, dominant negative mutation, and overexpression were utilized to modify Gαi2 expression or regulate its function. Their effects on endothelial cell functions were assessed in vitro . In vivo , the endothelial-specific Gαi2 shRNA adeno-associated virus (AAV) was utilized to silence Gαi2 expression. The impact of this suppression on retinal angiogenesis in control mice and streptozotocin (STZ)-induced diabetic retinopathy (DR) mice was analyzed...
2024: Theranostics
https://read.qxmd.com/read/38389853/the-mitochondria%C3%A2-paraspeckle-axis-regulates-the-survival-of-transplanted-stem-cells-under-oxidative-stress-conditions
#25
JOURNAL ARTICLE
Meng Zhao, Shuyun Liu, Yizhuo Wang, Ke Lv, Peng Lou, Pingya Zhou, Jiaying Zhu, Lan Li, Jingqiu Cheng, Yanrong Lu, Jingping Liu
Rationale: Stem cell-based therapies have emerged as promising tools for tissue engineering and regenerative medicine, but their therapeutic efficacy is largely limited by the oxidative stress-induced loss of transplanted cells at injured tissue sites. To address this issue, we aimed to explore the underlying mechanism and protective strategy of ROS-induced MSC loss. Methods: Changes in TFAM (mitochondrial transcription factor A) signaling, mitochondrial function, DNA damage, apoptosis and senescence in MSCs under oxidative stress conditions were assessed using real-time PCR, western blotting and RNA sequencing, etc...
2024: Theranostics
https://read.qxmd.com/read/38389852/magl-protects-against-renal-fibrosis-through-inhibiting-tubular-cell-lipotoxicity
#26
JOURNAL ARTICLE
Shan Zhou, Xian Ling, Jielin Zhu, Ye Liang, Qijian Feng, Chao Xie, Jiemei Li, Qiyan Chen, Shuangqin Chen, Jinhua Miao, Mengyao Zhang, Zhiru Li, Weiwei Shen, Xiaolong Li, Qinyu Wu, Xiaoxu Wang, Ruiyuan Liu, Cheng Wang, Fan Fan Hou, Yaozhong Kong, Youhua Liu, Lili Zhou
Rationale: Renal fibrosis, with no therapeutic approaches, is a common pathological feature in various chronic kidney diseases (CKD). Tubular cell injury plays a pivotal role in renal fibrosis. Commonly, injured tubular cells exhibit significant lipid accumulation. However, the underlying mechanisms remain poorly understood. Methods: 2-arachidonoylglycerol (2-AG) levels in CKD patients and CKD model specimens were measured using mass spectrometry. 2-AG-loaded nanoparticles were infused into unilateral ureteral obstruction (UUO) mice...
2024: Theranostics
https://read.qxmd.com/read/38389851/tripeptidyl-peptidase-ii-coordinates-the-homeostasis-of-calcium-and-lipids-in-the-central-nervous-system-and-its-depletion-causes-presenile-dementia-in-female-mice-through-calcium-lipid-dyshomeostasis-induced-autophagic-degradation-of-cyp19a1
#27
JOURNAL ARTICLE
Jin Zhao, Chengtong He, Xueyu Fan, Lin Wang, Liao Zhao, Hui Liu, Wujun Shen, Sanwei Jiang, Kaixuan Pei, Jingjing Gao, Yawei Qi, Yang Liu, Junqiang Zhao, Ruiling Zhang, Chengbiao Lu, Jia Tong, Jisen Huai
Rationale: Tripeptidyl peptidase II (TPP2) has been proven to be related to human immune and neurological diseases. It is generally considered as a cytosolic protein which forms the largest known protease complex in eukaryotic cells to operate mostly downstream of proteasomes for degradation of longer peptides. However, this canonical function of TPP2 cannot explain its role in a wide variety of biological and pathogenic processes. The mechanistic interrelationships and hierarchical order of these processes have yet to be clarified...
2024: Theranostics
https://read.qxmd.com/read/38389850/polycomb-repressive-complex-1-modulates-granulosa-cell-proliferation-in-early-folliculogenesis-to-support-female-reproduction
#28
JOURNAL ARTICLE
Meng Gao, Tuo Zhang, Tengxiang Chen, Ziqi Chen, Zijian Zhu, Yang Wen, Shaogang Qin, Yibing Bao, Ting Zhao, Hengxing Li, Longping Liu, Ming Hao, Jianbin Wang, Fengchao Wang, Haibin Wang, Bo Zhou, Hua Zhang, Guoliang Xia, Chao Wang
Rationale: Premature ovarian insufficiency (POI) is an accelerated reduction in ovarian function inducing infertility. Folliculogenesis defects have been reported to trigger POI as a consequence of ovulation failure. However, the underlying mechanisms remain unclear due to the genetic complexity and heterogeneity of POI. Methods: We used whole genome sequencing (WGS), conditional knockout mouse models combined with laser capture microdissection (LCM), and RNA/ChIP sequencing to analyze the crucial roles of polycomb repressive complex 1 (PRC1) in clinical POI and mammalian folliculogenesis...
2024: Theranostics
https://read.qxmd.com/read/38389849/re-analysis-of-single-cell-transcriptomics-reveals-a-critical-role-of-macrophage-like-smooth-muscle-cells-in-advanced-atherosclerotic-plaque
#29
JOURNAL ARTICLE
Xue Gong, Yunchang Liu, Huiying Liu, Nian Cao, Liping Zeng, Miao Tian, Chunyu Zeng, Yijie Hu, Runjun Zhang, Yundai Chen, Gengze Wu
Aims: Smooth muscle cell (SMC) remodeling poses a critical feature in the development and progression of atherosclerosis. Although fate mapping and in silicon approaches have expanded SMC phenotypes in atherosclerosis, it still remains elusive about the contributions of individual SMC phenotypes and molecular dynamics to advanced atherosclerotic plaque. Methods: Using single-cell transcriptome, we investigated cellular compositions of human carotid plaque laden with atherosclerotic core, followed by in vivo experiments utilizing SMC-lineage tracing technology, bulk RNA sequencing (RNA-seq) and both in vivo and in vitro validation of the underlying molecular mechanism...
2024: Theranostics
https://read.qxmd.com/read/38389848/20-hete-mediated-trpv1-activation-drives-allokinesis-via-mrgpra3-neurons-in-chronic-dermatitis
#30
JOURNAL ARTICLE
Guang Yu, Pei Liu, Xiaobao Huang, Mingxin Qi, Xue Li, Weimeng Feng, Erxin Shang, Yuan Zhou, Changming Wang, Yan Yang, Chan Zhu, Fang Wang, Zongxiang Tang, Jinao Duan
Rationale: Noxious stimuli are often perceived as itchy in patients with chronic dermatitis (CD); however, itch and pain mechanisms of CD are not known. Methods: TRPV1 involvement in CD was analyzed using a SADBE induced CD-like mouse model, and several loss- and gain-of-function mouse models. Trigeminal TRPV1 channel and MrgprA3+ neuron functions were analyzed by calcium imaging and whole-cell patch-clamp recordings. Lesional CD-like skin from mice were analyzed by unbiased metabolomic analysis. 20-HETE availability in human and mouse skin were determined by LC/MS and ELISA...
2024: Theranostics
https://read.qxmd.com/read/38389847/high-content-stimulated-raman-histology-of-human-breast-cancer
#31
JOURNAL ARTICLE
Hongli Ni, Chinmayee Prabhu Dessai, Haonan Lin, Wei Wang, Shaoxiong Chen, Yuhao Yuan, Xiaowei Ge, Jianpeng Ao, Nolan Vild, Ji-Xin Cheng
Histological examination is crucial for cancer diagnosis, however, the labor-intensive sample preparation involved in the histology impedes the speed of diagnosis. Recently developed two-color stimulated Raman histology could bypass the complex tissue processing to generates result close to hematoxylin and eosin staining, which is one of the golden standards in cancer histology. Yet, the underlying chemical features are not revealed in two-color stimulated Raman histology, compromising the effectiveness of prognostic stratification...
2024: Theranostics
https://read.qxmd.com/read/38389846/mrc1-macrophage-derived-igf1-mitigates-crystal-nephropathy-by-promoting-renal-tubule-cell-proliferation-via-the-akt-rb-signaling-pathway
#32
JOURNAL ARTICLE
Linxi Huang, Wei Chen, Zhuojing Tan, Yunxiao Huang, Xinji Gu, Lantian Liu, Hongxia Zhang, Yihan Shi, Jiarong Ding, Chengjian Zheng, Zhiyong Guo, Bing Yu
Rationale: The present understanding of the cellular characteristics and communications in crystal nephropathy is limited. Here, molecular and cellular studies combined with single-cell RNA sequencing (scRNA-seq) were performed to investigate the changes in cell components and their interactions in glyoxylate-induced crystallized kidneys to provide promising treatments for crystal nephropathy. Methods: The transcriptomes of single cells from mouse kidneys treated with glyoxylate for 0, 1, 4, or 7 days were analyzed via 10× Genomics, and the single cells were clustered and characterized by the Seurat pipeline...
2024: Theranostics
https://read.qxmd.com/read/38389845/metal-nanoparticle-hybrid-hydrogels-the-state-of-the-art-of-combining-hard-and-soft-materials-to-promote-wound-healing
#33
REVIEW
Yuxiang Wang, Mengya Zhang, Zhenzhen Yan, Shizhao Ji, Shichu Xiao, Jie Gao
Wounds represent a grave affliction that profoundly impacts human well-being. Establishing barriers, preventing infections, and providing a conducive microenvironment constitute the crux of wound therapy. Hydrogel, a polymer with an intricate three-dimensional lattice, serves as a potent tool in erecting physical barriers and nurturing an environment conducive to wound healing. This enables effective control over exudation, hemostasis, accelerated wound closure, and diminished scar formation. As a result, hydrogels have gained extensive traction in the realm of wound treatment...
2024: Theranostics
https://read.qxmd.com/read/38389844/degraders-in-epigenetic-therapy-protacs-and-beyond
#34
REVIEW
Xing-Jie Dai, Shi-Kun Ji, Meng-Jie Fu, Gao-Zhi Liu, Hui-Min Liu, Shao-Peng Wang, Liang Shen, Ning Wang, Piet Herdewijn, Yi-Chao Zheng, Sai-Qi Wang, Xiao-Bing Chen
Epigenetics refers to the reversible process through which changes in gene expression occur without changing the nucleotide sequence of DNA. The process is currently gaining prominence as a pivotal objective in the treatment of cancers and other ailments. Numerous drugs that target epigenetic mechanisms have obtained approval from the Food and Drug Administration (FDA) for the therapeutic intervention of diverse diseases; many have drawbacks, such as limited applicability, toxicity, and resistance. Since the discovery of the first proteolysis-targeting chimeras (PROTACs) in 2001, studies on targeted protein degradation (TPD)-encompassing PROTACs, molecular glue (MG), hydrophobic tagging (HyT), degradation TAG (dTAG), Trim-Away, a specific and non-genetic inhibitor of apoptosis protein (IAP)-dependent protein eraser (SNIPER), antibody-PROTACs (Ab-PROTACs), and other lysosome-based strategies-have achieved remarkable progress...
2024: Theranostics
https://read.qxmd.com/read/38389843/terbium-radionuclides-for-theranostic-applications-in-nuclear-medicine-from-atom-to-bedside
#35
REVIEW
Camille Van Laere, Michel Koole, Christophe M Deroose, Michiel Van de Voorde, Kristof Baete, Thomas E Cocolios, Charlotte Duchemin, Maarten Ooms, Frederik Cleeren
Terbium features four clinically interesting radionuclides for application in nuclear medicine: terbium-149, terbium-152, terbium-155, and terbium-161. Their identical chemical properties enable the synthesis of radiopharmaceuticals with the same pharmacokinetic character, while their distinctive decay characteristics make them valuable for both imaging and therapeutic applications. In particular, terbium-152 and terbium-155 are useful candidates for positron emission tomography (PET) and single photon emission computed tomography (SPECT) imaging, respectively; whereas terbium-149 and terbium-161 find application in α- and β- -/Auger electron therapy, respectively...
2024: Theranostics
https://read.qxmd.com/read/38389842/emerging-role-of-extracellular-vesicles-in-diabetic-retinopathy
#36
REVIEW
Junyan Zhu, Jin Huang, Yaoxiang Sun, Wenrong Xu, Hui Qian
Diabetic retinopathy (DR), a complex complication of diabetes mellitus (DM), is a leading cause of adult blindness. Hyperglycemia triggers DR, resulting in microvascular damage, glial apoptosis, and neuronal degeneration. Inflammation and oxidative stress play crucial roles during this process. Current clinical treatments for DR primarily target the advanced retinal disorder but offer limited benefits with inevitable side effects. Extracellular vesicles (EVs) exhibit unique morphological features, contents, and biological properties and can be found in cell culture supernatants, various body fluids, and tissues...
2024: Theranostics
https://read.qxmd.com/read/38389841/transfer-rna-derived-fragment-trf-glu-ctc-aggravates-the-development-of-neovascular-age-related-macular-degeneration
#37
JOURNAL ARTICLE
Yu Liang, Lingjie Kong, Yuelu Zhang, Yihan Zhang, Mingsu Shi, Jiaqiu Huang, Hongyu Kong, Siyi Qi, Yunlong Yang, Jiaxu Hong, Meidong Zhu, Xiangjia Zhu, Xinghuai Sun, Shujie Zhang, Lianqun Wu, Chen Zhao
Rationale: Angiogenesis expedites tissue impairment in many diseases, including age-related macular degeneration (AMD), a leading cause of irreversible blindness in elderly. A substantial proportion of neovascular AMD patients, characterized by aberrant choroidal neovascularization (CNV), exhibit poor responses or adverse reactions to anti-VEGF therapy. Herein, we aimed to unveil the function of newly identified transfer RNA-derived small RNA, tRF-Glu-CTC, in the pathology of CNV and determine its potential in inhibiting angiogenesis...
2024: Theranostics
https://read.qxmd.com/read/38389840/global-metabolomics-revealed-deviations-from-the-metabolic-aging-clock-in-colorectal-cancer-patients
#38
JOURNAL ARTICLE
Long Zhang, Shaobo Mo, Xiurui Zhu, C James Chou, Bo Jin, Zhi Han, James Schilling, Weili Liao, Sheeno Thyparambil, Ruben Y Luo, John C Whitin, Lu Tian, Seema Nagpal, Scott R Ceresnak, Harvey J Cohen, Doff B McElhinney, Karl G Sylvester, Yangming Gong, Chen Fu, Xuefeng B Ling, Junjie Peng
Background: Markers of aging hold promise in the context of colorectal cancer (CRC) care. Utilizing high-resolution metabolomic profiling, we can unveil distinctive age-related patterns that have the potential to predict early CRC development. Our study aims to unearth a panel of aging markers and delve into the metabolomic alterations associated with aging and CRC. Methods: We assembled a serum cohort comprising 5,649 individuals, consisting of 3,002 healthy volunteers, 715 patients diagnosed with colorectal advanced precancerous lesions (APL), and 1,932 CRC patients, to perform a comprehensive metabolomic analysis...
2024: Theranostics
https://read.qxmd.com/read/38389839/cancer-associated-fibroblasts-reprogram-cysteine-metabolism-to-increase-tumor-resistance-to-ferroptosis-in-pancreatic-cancer
#39
JOURNAL ARTICLE
Yuchao Zhu, Shuai Fang, Bolin Fan, Kaiwei Xu, Liu Xu, Linwei Wang, Lubin Zhu, Chunqu Chen, Ruoyu Wu, Jiajing Ni, Jianhua Wang
Background: Pancreatic ductal adenocarcinoma (PDAC) is an insidious, rapidly progressing malignancy of the gastrointestinal tract. Due to its dense fibrous stroma and complex tumor microenvironment, neither of which is sensitive to radiotherapy, pancreatic adenocarcinoma is one of the malignancies with the poorest prognosis. Therefore, detailed elucidation of the inhibitory microenvironment of PDAC is essential for the development of novel therapeutic strategies. Methods: We analyzed the association between cancer-associated fibroblasts (CAFs) and resistance to ferroptosis in PDAC using conditioned CAF medium and co-culture of pancreatic cancer cells...
2024: Theranostics
https://read.qxmd.com/read/38389838/focused-ultrasound-ablation-of-melanoma-with-boiling-histotripsy-yields-abscopal-tumor-control-and-antigen-dependent-dendritic-cell-activation
#40
JOURNAL ARTICLE
Eric A Thim, Lydia E Kitelinger, Fátima Rivera-Escalera, Alexander S Mathew, Michael R Elliott, Timothy N J Bullock, Richard J Price
Background: Boiling histotripsy (BH), a mechanical focused ultrasound ablation strategy, can elicit intriguing signatures of anti-tumor immunity. However, the influence of BH on dendritic cell function is unknown, compromising our ability to optimally combine BH with immunotherapies to control metastatic disease. Methods: BH was applied using a sparse scan (1 mm spacing between sonications) protocol to B16F10-ZsGreen melanoma in bilateral and unilateral settings. Ipsilateral and contralateral tumor growth was measured...
2024: Theranostics
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