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Journals Biotechnology Research Interna...

Biotechnology Research International

https://read.qxmd.com/read/29610687/potential-applications-of-some-indigenous-bacteria-isolated-from-polluted-areas-in-the-treatment-of-brewery-effluents
#1
JOURNAL ARTICLE
Temesgen Oljira, Diriba Muleta, Mulissa Jida
Biological wastewater treatment is economically feasible and ecofriendly. This study was aimed at isolating bacteria from brewery wastes and evaluating their bioremediation potential as individual isolate and/or their consortium in reducing the pollutants of brewery effluents. A total of 40 bacterial isolates were recovered and of these the three best isolates were selected. The selected bacteria were identified to genus level by using morphological and biochemical characteristics. Accordingly, the isolates were identified as Aeromonas sp...
2018: Biotechnology Research International
https://read.qxmd.com/read/29387490/selection-and-characterization-of-potential-baker-s-yeast-from-indigenous-resources-of-nepal
#2
JOURNAL ARTICLE
Tika B Karki, Parash Mani Timilsina, Archana Yadav, Gyanu Raj Pandey, Yogesh Joshi, Sahansila Bhujel, Rojina Adhikari, Katyayanee Neupane
The study aims to isolate the yeast strains that could be used effectively as baker's yeast and compare them with the commercial baker's yeast available in the market of Nepal. A total of 10 samples including locally available sources like fruits, Murcha, and a local tree "Dar" were collected from different localities of Bhaktapur, Kavre, and Syangja districts of Nepal, respectively. Following enrichment and fermentation of the samples, 26 yeast strains were isolated using selective medium Wallerstein Laboratory Nutrient Agar...
2017: Biotechnology Research International
https://read.qxmd.com/read/28620550/generation-of-recombinant-antibodies-against-the-beta-1-6-branched-beta-1-3-d-glucan-schizophyllan-from-immunized-mice-via-phage-display
#3
JOURNAL ARTICLE
Jörn Josewski, Sabine Buchmeier, André Frenzel, Philip Tinnefeld, Stefan Dübel, Udo Rau
beta-(1,6)-Branched beta-(1,3)-D-glucans like schizophyllan from the basidiomycete Schizophyllum commune excite various immunostimulatory effects and have been clinically tested as adjuvants. Some of the glucans are also applicable in food or petrol industry due to their viscosity and temperature stability in aqueous solution. Antibodies against these glucans could be used as tool for analysis of glucan preparations or for further research of its bioactivity. Therefore, an immune phage display library was constructed from mice immunized with schizophyllan...
2017: Biotechnology Research International
https://read.qxmd.com/read/28116165/optimization-of-crude-oil-and-pahs-degradation-by-stenotrophomonas-rhizophila-kx082814-strain-through-response-surface-methodology-using-box-behnken-design
#4
JOURNAL ARTICLE
Praveen Kumar Siddalingappa Virupakshappa, Manjunatha Bukkambudhi Krishnaswamy, Gaurav Mishra, Mohammed Ameenuddin Mehkri
The present paper describes the process optimization study for crude oil degradation which is a continuation of our earlier work on hydrocarbon degradation study of the isolate Stenotrophomonas rhizophila (PM-1) with GenBank accession number KX082814 . Response Surface Methodology with Box-Behnken Design was used to optimize the process wherein temperature, pH, salinity, and inoculum size (at three levels) were used as independent variables and Total Petroleum Hydrocarbon, Biological Oxygen Demand, and Chemical Oxygen Demand of crude oil and PAHs as dependent variables (response)...
2016: Biotechnology Research International
https://read.qxmd.com/read/27812388/corrigendum-to-proteases-from-canavalia-ensiformis-active-and-thermostable-enzymes-with-potential-of-application-in-biotechnology
#5
Rayane Natashe Gonçalves, Suellen Duarte Gozzini Barbosa, Raquel Elisa da Silva-López
[This corrects the article DOI: 10.1155/2016/3427098.].
2016: Biotechnology Research International
https://read.qxmd.com/read/27721994/influence-of-halide-solutions-on-collagen-networks-measurements-of-physical-properties-by-atomic-force-microscopy
#6
JOURNAL ARTICLE
Birgit Spitzer-Sonnleitner, André Kempe, Maximilian Lackner
The influence of aqueous halide solutions on collagen coatings was tested. The effects on resistance against indentation/penetration on adhesion forces were measured by atomic force microscopy (AFM) and the change of Young's modulus of the coating was derived. Comparative measurements over time were conducted with halide solutions of various concentrations. Physical properties of the mesh-like coating generally showed large variability. Starting with a compact set of physical properties, data disperse after minutes...
2016: Biotechnology Research International
https://read.qxmd.com/read/27699070/effect-of-purified-mushroom-tyrosinase-on-melanin-content-and-melanogenic-protein-expression
#7
JOURNAL ARTICLE
Kamal Uddin Zaidi, Sharique A Ali, Ayesha S Ali
In mammalian melanocytes, melanosome is a highly specialized organelle where melanin is synthesized. Melanin synthesis is controlled by tyrosinase, the vital enzyme in melanogenic pathway. The present investigation is based on an effect of purified mushroom tyrosinase of Agaricus bisporus on B16F10 melanocytes for the melanin production via blocking pigment cell machinery. Using B16F10 melanocytes showed that the stimulation of melanogenesis by purified tyrosinase is due to increased tyrosinase absorption. Cellular tyrosinase activity and melanin content in B16F10 melanocytes were increased by purified tyrosinase in a dose-dependent manner...
2016: Biotechnology Research International
https://read.qxmd.com/read/27648307/%C3%AE-cyclodextrin-production-by-cyclodextrin-glucanotransferase-from-an-alkaliphile-microbacterium-terrae-knr-9-using-different-starch-substrates
#8
Kiransinh N Rajput, Kamlesh C Patel, Ujjval B Trivedi
Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an important member of α-amylase family which can degrade the starch and produce cyclodextrins (CDs) as a result of intramolecular transglycosylation (cyclization). β-Cyclodextrin production was carried out using the purified CGTase enzyme from an alkaliphile Microbacterium terrae KNR 9 with different starches in raw as well as gelatinized form. Cyclodextrin production was confirmed using thin layer chromatography. Six different starch substrates, namely, soluble starch, potato starch, sago starch, corn starch, corn flour, and rice flour, were tested for CD production...
2016: Biotechnology Research International
https://read.qxmd.com/read/27630776/proteases-from-canavalia-ensiformis-active-and-thermostable-enzymes-with-potential-of-application-in-biotechnology
#9
JOURNAL ARTICLE
Rayane Natshe Gonçalves, Suellen Duarte Gozzini Barbosa, Raquel Elisa da Silva-López
Extracts of leaves, seeds, roots, and stem from a tropical legume, C. ensiformis, were prepared employing buffers and detergent in aqueous solution. Leaf extracts had the highest protein content and the most pronounced peptidase activity with optimal pH in the neutral to alkaline range. All extracts exhibited peaks of activity at various pH values, suggesting the presence of distinctive classes of proteases. N-α-Tosyl-L-arginine methyl ester hydrolysis was maximal at 30°C to 60°C and peptidase activity from all extracts presented very good thermal stability after 24 h incubation at 70°C...
2016: Biotechnology Research International
https://read.qxmd.com/read/27242929/the-use-of-adenovirus-dodecahedron-in-the-delivery-of-an-enzymatic-activity-in-the-cell
#10
JOURNAL ARTICLE
Sumarheni, Benoit Gallet, Pascal Fender
Penton-dodecahedron (Pt-Dd) derived from adenovirus type 3 is a symmetric complex of pentameric penton base plus fiber which can be produced in the baculovirus system at a high concentration. The size of Pt-Dd is smaller than the virus, but this virus-like particle (VLP) has the major proteins recognized by specific receptors on the surface of almost all types of cell. In this study, by direct observation with fluorescence microscopy on a fixed and living cell, the intracellular trafficking and localization of Pt-Dd labeled with fluorescence dyes in the cytoplasm of HeLa Tub-GFP showed a rapid internalization characteristic...
2016: Biotechnology Research International
https://read.qxmd.com/read/27006832/chimerism-analysis-of-cell-free-dna-in-patients-treated-with-hematopoietic-stem-cell-transplantation-may-predict-early-relapse-in-patients-with-hematologic-malignancies
#11
JOURNAL ARTICLE
Mahmoud Aljurf, Hala Abalkhail, Amal Alseraihy, Said Y Mohamed, Mouhab Ayas, Fahad Alsharif, Hazza Alzahrani, Abdullah Al-Jefri, Ghuzayel Aldawsari, Ali Al-Ahmari, Asim F Belgaumi, Claudia Ulrike Walter, Hassan El-Solh, Walid Rasheed, Maher Albitar
Background. We studied DNA chimerism in cell-free DNA (cfDNA) in patients treated with HSCT. Methods. Chimerism analysis was performed on CD3+ cells, polymorphonuclear (PMN) cells, and cfDNA using 16 small tandem repeat loci. The resulting labeled PCR-products were size-fractionated and quantified. Results. Analyzing samples from 191 patients treated with HSCT for nonneoplastic hematologic disorders demonstrated that the cfDNA chimerism is comparable to that seen in PMN cells. Analyzing leukemia patients (N = 126) showed that, of 84 patients with 100% donor DNA in PMN, 16 (19%) had evidence of clinical relapse and >10% recipient DNA in the plasma...
2016: Biotechnology Research International
https://read.qxmd.com/read/26955489/screening-and-selection-of-medium-components-for-cyclodextrin-glucanotransferase-production-by-new-alkaliphile-microbacterium-terrae-knr-9-using-plackett-burman-design
#12
JOURNAL ARTICLE
Kiransinh N Rajput, Kamlesh C Patel, Ujjval B Trivedi
Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) production using new alkaliphile Microbacterium terrae KNR 9 was investigated by submerged fermentation. Statistical screening for components belonging to different categories, namely, soluble and raw starches as carbon sources, complex organic and inorganic nitrogen sources, minerals, a buffering agent, and a surfactant, has been carried out for CGTase production using Plackett-Burman factorial design. To screen out k (19), number of variables, k + 1 (20), number of experiments, were performed...
2016: Biotechnology Research International
https://read.qxmd.com/read/26942014/utilization-of-crude-glycerol-as-a-substrate-for-the-production-of-rhamnolipid-by-pseudomonas-aeruginosa
#13
JOURNAL ARTICLE
Walaa A Eraqi, Aymen S Yassin, Amal E Ali, Magdy A Amin
Biosurfactants are produced by bacteria or yeast utilizing different substrates as sugars, glycerol, or oils. They have important applications in the detergent, oil, and pharmaceutical industries. Glycerol is the product of biodiesel industry and the existing glycerol market cannot accommodate the excess amounts generated; consequently, new markets for refined glycerol need to be developed. The aim of present work is to optimize the production of microbial rhamnolipid using waste glycerol. We have developed a process for the production of rhamnolipid biosurfactants using glycerol as the sole carbon source by a local Pseudomonas aeruginosa isolate that was obtained from an extensive screening program...
2016: Biotechnology Research International
https://read.qxmd.com/read/26904295/synergistic-effects-of-plant-growth-promoting-rhizobacteria-and-chitosan-on-in-vitro-seeds-germination-greenhouse-growth-and-nutrient-uptake-of-maize-zea-mays-l
#14
JOURNAL ARTICLE
Nadège A Agbodjato, Pacôme A Noumavo, Adolphe Adjanohoun, Léonce Agbessi, Lamine Baba-Moussa
This study aimed to assess the effects of three plant growth promoting rhizobacteria (PGPR) and chitosan either singly or in combination on maize seeds germination and growth and nutrient uptake. Maize seeds were treated with chitosan and bacterial solution. The germination and growth tests were carried out in square Petri dishes and plastic pots. The combination chitosan-A. lipoferum-P. fluorescens has increased the seeds vigor index up to 36.44% compared to the control. In comparison to the control, P. putida has significantly improved root weight (44...
2016: Biotechnology Research International
https://read.qxmd.com/read/26881084/role-of-heat-shock-proteins-in-cellular-function-and-in-the-biology-of-fungi
#15
REVIEW
Shraddha Tiwari, Raman Thakur, Jata Shankar
Stress (biotic or abiotic) is an unfavourable condition for an organism including fungus. To overcome stress, organism expresses heat-shock proteins (Hsps) or chaperons to perform biological function. Hsps are involved in various routine biological processes such as transcription, translation and posttranslational modifications, protein folding, and aggregation and disaggregation of proteins. Thus, it is important to understand holistic role of Hsps in response to stress and other biological conditions in fungi...
2015: Biotechnology Research International
https://read.qxmd.com/read/26839707/prospects-for-irradiation-in-cellulosic-ethanol-production
#16
REVIEW
Anita Saini, Neeraj K Aggarwal, Anuja Sharma, Anita Yadav
Second generation bioethanol production technology relies on lignocellulosic biomass composed of hemicelluloses, celluloses, and lignin components. Cellulose and hemicellulose are sources of fermentable sugars. But the structural characteristics of lignocelluloses pose hindrance to the conversion of these sugar polysaccharides into ethanol. The process of ethanol production, therefore, involves an expensive and energy intensive step of pretreatment, which reduces the recalcitrance of lignocellulose and makes feedstock more susceptible to saccharification...
2015: Biotechnology Research International
https://read.qxmd.com/read/26779347/response-surface-optimization-of-bioethanol-production-from-sugarcane-molasses-by-pichia-veronae-strain-hsc-22
#17
JOURNAL ARTICLE
Hamed I Hamouda, Hussein N Nassar, Hekmat R Madian, Salem S Abu Amr, Nour Sh El-Gendy
Pichia veronae strain HSC-22 (accession number KP012558) showed a good tolerance to relatively high temperature, ethanol and sugar concentrations. Response surface optimization based on central composite design of experiments predicted the optimal values of the influencing parameters that affect the production of bioethanol from sugarcane molasses to be as follows: initial pH 5, 25% (w : v) initial molasses concentration, 35°C, 116 rpm, and 60 h. Under these optimum operating conditions the maximum bioethanol production on a batch fermenter scale was recorded as 32...
2015: Biotechnology Research International
https://read.qxmd.com/read/26770830/enhanced-bioremediation-of-soil-artificially-contaminated-with-petroleum-hydrocarbons-after-amendment-with-capra-aegagrus-hircus-goat-manure
#18
JOURNAL ARTICLE
T P Nwogu, C C Azubuike, C J Ogugbue
This study was carried out to evaluate the biostimulant potentials of Capra aegagrus hircus manure for bioremediation of crude oil contaminated soil (COCS) under tropical conditions. 1 kg of COCS sample was amended with 0.02 kg of C. a. hircus manure and monitored at 14-day intervals for total petroleum hydrocarbon (TPH), nutrient content, and changes in microbial counts. At the end of the study period, there was 62.08% decrease in the concentration of TPH in the amended sample compared to 8.15% decrease in the unamended sample, with significant differences (P < 0...
2015: Biotechnology Research International
https://read.qxmd.com/read/26697226/plant-growth-promotion-activity-of-keratinolytic-fungi-growing-on-a-recalcitrant-waste-known-as-hair-waste
#19
JOURNAL ARTICLE
Ivana A Cavello, Juan M Crespo, Sabrina S García, José M Zapiola, María F Luna, Sebastián F Cavalitto
Purpureocillium lilacinum (Thom) Samsom is one of the most studied fungi in the control of plant parasitic nematodes. However, there is not specific information on its ability to inhibit some pathogenic bacteria, fungi, or yeast. This work reports the production of several antifungal hydrolytic enzymes by a strain of P. lilacinum when it is grown in a medium containing hair waste. The growth of several plant-pathogenic fungi, Alternaria alternata, Aspergillus niger, and Fusarium culmorum, was considerably affected by the presence of P...
2015: Biotechnology Research International
https://read.qxmd.com/read/26691554/purification-characterization-of-l-methioninase-from-candida-tropicalis-and-its-application-as-an-anticancer
#20
JOURNAL ARTICLE
Mohsen Helmy Selim, El-Zahraa Karm Eldin, Moataza Mahmoud Saad, El-Sayed Eliwa Mostafa, Yosrea Hassan Shetia, Amany Ahmed Hassabo Anise
The aim of the present study is to purify L-methioninase from Candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular weight of 46 KDa. Its optimum temperature was 45 to 55 and thermal stability was 55°C for 15 min. The enzyme had optimum pH at 6.5 and stability at a pH range of 5.5 to 7.0 for 24 hr. The maximum activity was observed with substrate concentration of 30 µM and Km was 0...
2015: Biotechnology Research International
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