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Analytical Cellular Pathology (Amsterdam)

Hui Du, Yun Le, Fenyong Sun, Kai Li, Yanfeng Xu
Cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) is overexpressed and has an oncogenic role in hepatocellular carcinoma (HCC). Interleukin enhancer binding factor 2 (ILF2) has become research hotspot in liver cancer recently. However, it is still unclear whether and how CREB and ILF2 interact with each other. And how this interaction exerts its role in occurrence and development of liver cancer is still unclear. Here, we found that ILF2 directly bound with CREB, and this binding was essential for the malignant phenotypes of liver cancer cells...
2019: Analytical Cellular Pathology (Amsterdam)
Xuting Xu, Dong Li, Jin Liu, Zhihong Ma, Huilian Huang, Lishan Min, Licheng Dai, Shunli Dong
Objective: The receptor-type tyrosine-protein phosphatase κ (PTPRK) is a candidate tumor suppressor involved in the tumorigenesis of various organs. However, its expression and biological roles in non-small-cell lung cancer (NSCLC) have not yet been investigated. Methods: PTPRK expression in NSCLC tissues and cell lines was examined using real-time PCR and western blotting. In addition, the effects of PTPRK on cell migration, invasion, and proliferation were evaluated in vitro ...
2019: Analytical Cellular Pathology (Amsterdam)
Xiangyu Kong, Ganghao Ai, Dai Wang, Renzhen Chen, Dongbei Guo, Youliang Yao, Kai Wang, Guiye Liang, Fengjie Qi, Wenzhi Liu, Yongxing Zhang
Objective: To assess the expression levels of exchange protein 1 directly activated by cAMP (Epac1) and phosphodiesterase 4 (PDE4) in rectal carcinoma, and their associations with clinicopathological indexes. In addition, the associations of PDE4 and Epac1 with A-kinase anchor protein 95, connexin 43, cyclin D1, and cyclin E1 were evaluated. Methods: The PV-9000 two-step immunohistochemistry method was used to determine protein expression in 44 rectal carcinoma tissue samples and 16 paracarcinoma tissue specimens...
2019: Analytical Cellular Pathology (Amsterdam)
Geehyuk Kim, Jemberu Taye, Kwangmin Yu, Sunyoung Park, Jungho Kim, Sunghyun Kim, Dongsup Lee, Hye-Young Wang, Kwang Hwa Park, Hyeyoung Lee
After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis. Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods...
2019: Analytical Cellular Pathology (Amsterdam)
Sun Eui Kim, Hye Jung Baek, Eun Jung Park, Sung Chul Lim, Sang Soo Kim
Environmental and genetic factors exert important influences on lifespan and neoplastic transformation. We have previously shown that spontaneous tumors form frequently in mice homozygous for a full-length Brca1 deletion. In general, mutations of BRCA1 are closely associated with induction of breast and ovarian cancers but are also known to contribute to the incidence of other cancers at a low frequency. Female Brca1 -mutant mice ( Brca1co/co MMTV-cre ) were generated by crossing Brca1 conditional knockout mice and MMTV-cre mice, and the occurrence of lacrimal gland abnormalities and tumors was followed until mice reached 18 months of age...
2018: Analytical Cellular Pathology (Amsterdam)
Mikhail T Silk, Nina Mikkilineni, Tarik C Silk, Emily C Zabor, Irina Ostrovnaya, Ari A Hakimi, James J Hsieh, Etay Ziv, Natasha Rekhtman, Stephen B Solomon, Jeremy C Durack
Context: Targeted needle biopsies are increasingly performed for the genetic characterization of cancer. While the nucleic acid content of core needle biopsies after standard pathology processing (i.e., formalin fixation and paraffin embedding (FFPE)) has been previously reported, little is known about the potential yield for molecular analysis at the time of biopsy sample acquisition. Objectives: Our objective was to improve the understanding of DNA and RNA yields from commonly used core needle biopsy techniques prior to sample processing...
2018: Analytical Cellular Pathology (Amsterdam)
Isaac Tuffour, Irene Ayi, Theresa Manful Gwira, Edward Dumashie, Yvonne Ashong, Regina Appiah-Opong
Schistosomiasis is a neglected tropical disease that affects 200 million people and accounts for 100,000 deaths annually. In endemic geographical areas, schistosomiasis has been implicated as an etiological agent in the pathogenesis of bladder, colorectal, and renal carcinoma largely due to Schistosoma eggs in tissues that comes with chronic infection. Several studies have also reported cases of association between Schistosoma infection and prostate cancer. The possible causal association is however poorly understood...
2018: Analytical Cellular Pathology (Amsterdam)
Stefano Castellani, Sante Di Gioia, Lorena di Toma, Massimo Conese
Chronic inflammation, oxidative stress, mucus plugging, airway remodeling, and respiratory infections are the hallmarks of the cystic fibrosis (CF) lung disease. The airway epithelium is central in the innate immune responses to pathogens colonizing the airways, since it is involved in mucociliary clearance, senses the presence of pathogens, elicits an inflammatory response, orchestrates adaptive immunity, and activates mesenchymal cells. In this review, we focus on cellular models of the human CF airway epithelium that have been used for studying mucus production, inflammatory response, and airway remodeling, with particular reference to two- and three-dimensional cultures that better recapitulate the native airway epithelium...
2018: Analytical Cellular Pathology (Amsterdam)
Bo Wu, Haihua Yang, Shenpeng Ying, Hongsheng Lu, Wei Wang, Jiaming Lv, Huacai Xiong, Wei Hu
Background and Aims: In patients with nasopharyngeal carcinoma (NPC), local treatment failure and distant metastasis contribute largely to poor outcomes. The nasopharynx is an important lymphoid tissue, and NPC tumourigenesis and development are partly attributed to immune system disorders. Human leukocyte antigen F (HLA-F) has shown a close correlation with NPC in many genome-wide association studies (GWASs). However, clinical studies rarely explore the relationship of HLA-F expression with the clinical parameters and outcomes in patients with NPC...
2018: Analytical Cellular Pathology (Amsterdam)
Vladimir Jurišić, Jasmina Obradovic, Sonja Pavlović, Nataša Djordjevic
Recently, epidermal growth factor receptor (EGFR) was a key molecule in investigation of lung cancer, and it was a target for a new therapeutic strategy, based on molecular analyses. In this review, we have summarized some issues considering the role of EGFR in lung cancer, its coding gene, and its promoter gene polymorphisms (SNPs) -216G/T and -191C/A in non-small-cell lung cancer (NSCLC). The position of the SNPs indicates their significant role in EGFR regulation. The accumulation of knowledge regarding SNPs lately suggests their significant and important role in the onset of carcinogenesis, the prediction of the onset of metastases, the response to therapy with TKI inhibitors, and the onset of toxic effects of the applied therapy...
2018: Analytical Cellular Pathology (Amsterdam)
Alexander Glassmann, Carmen Carrillo Garcia, Viktor Janzen, Dominik Kraus, Nadine Veit, Jochen Winter, Rainer Probstmeier
Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state...
2018: Analytical Cellular Pathology (Amsterdam)
Sebastian Marciniak, Artur Wnorowski, Katarzyna Smolińska, Beata Walczyna, Waldemar Turski, Tomasz Kocki, Piotr Paluszkiewicz, Jolanta Parada-Turska
Acute liver failure (ALF) is a life-threatening disorder of liver function. Kynurenic acid (KYNA), a tryptophan metabolite formed along the kynurenine metabolic pathway, possesses anti-inflammatory and antioxidant properties. Its presence in food and its potential role in the digestive system was recently reported. The aim of this study was to define the effect of KYNA on liver failure. The Wistar rat model of thioacetamide-induced liver injury was used. Morphological and biochemical analyses as well as the measurement of KYNA content in liver and hepatoprotective herbal remedies were conducted...
2018: Analytical Cellular Pathology (Amsterdam)
Diyou Li, Yinglin Pan, Yating Huang, Ping Zhang, Xuhong Fang
Ovarian cancer is the most lethal gynecologic cancer and currently ranks fifth in causing cancer-related deaths among women. P21cdc42/rac1 -activated kinase 5 (PAK5) is a newly identified protein that has been indicated to have oncogenic potential. The present study investigated the expression level of PAK5 in clinical ovarian cancer and the functional roles of PAK5 in ovarian cancer progression. It was initially found that PAK5 was highly expressed in ovarian cancer tissues, particularly in patients with distant metastasis...
2018: Analytical Cellular Pathology (Amsterdam)
Sonja S Mojsilovic, Slavko Mojsilovic, Suncica Bjelica, Juan F Santibanez
Transforming growth factor-beta (TGF- β ) has been demonstrated as a key regulator of immune responses including monocyte/macrophage functions. TGF- β regulates macrophage cell migration and polarization, as well as it is shown to modulate macrophage urokinase-type plasminogen activator (uPA) production, which also contributes to macrophage chemotaxis and migration toward damaged or inflamed tissues. Microtubule (MT) cytoskeleton dynamic plays a key role during the cell motility, and any interference on the MT network profoundly affects cell migration...
2018: Analytical Cellular Pathology (Amsterdam)
Bushra Nissar, Showkat A Kadla, Nuzhat Shaheen Khan, Idrees A Shah, Misbah Majid, Falaque Ul Afshan, Bashir Ahmad Ganai
Coding polymorphisms in several DNA repair genes have been reported to affect the DNA repair capacity and are associated with genetic susceptibility to many human cancers, including gastric cancer. An understanding of these DNA repair gene polymorphisms might assess not only the risk of humans exposed to environmental carcinogens but also their responses to different therapeutical approaches, which target the DNA repair pathway. In the present study, polymorphic variants of two DNA repair genes, XRCC1 Arg399Gln and XPD Lys751Gln, were chosen to be studied in association with gastric cancer susceptibility in the Kashmiri population...
2018: Analytical Cellular Pathology (Amsterdam)
Emina Babarović, Ivan Franin, Marko Klarić, Ani Mihaljević Ferrari, Ružica Karnjuš-Begonja, Senija Eminović, Damjana Verša Ostojić, Danijela Vrdoljak-Mozetič
Objective: Adult granulosa cell tumors (AGCTs) represent 2%-5% of all ovarian malignancies. The aim of this study was to analyze clinical and pathohistological parameters and their impact on recurrence, overall, and disease-free survival in FIGO stage I AGCT patients. Methods: The tumor specimens analyzed in this retrospective study were obtained from a total of 36 patients with diagnosis of ovarian AGCT surgically treated at the Department of Gynecology, Rijeka University Hospital Centre, between 1994 and 2012...
2018: Analytical Cellular Pathology (Amsterdam)
Sellamuthu Subbanna Gounder, Basri Johan Jeet Abdullah, Nur Ezzati Izyan Binti Mohd Radzuanb, Farah Dalila Binti Mohd Zain, Nurhidayah Bt Mohamad Sait, Corine Chua, Baskar Subramani
Age-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two types, such as CD56bright and CD56dim cells, which represent cytokine production and cytotoxicity. In our study, we have examined the age-associated changes in the NK cell population and their subsets at different age groups of males and females (at a range from 41 to 80 years)...
2018: Analytical Cellular Pathology (Amsterdam)
Jenefa L Canoy, Jayzon G Bitacura
Brown macroalgae species are constantly reported as potential sources of bioactive compounds useful in inhibiting cell proliferation and vascular formation. Thus, this study was conducted to determine and compare the in vitro cytotoxic activities of Turbinaria ornata Agardh and Padina australis Hauck ethanolic extracts against baker's yeast ( Saccharomyces cerevisiae ) using the resazurin reduction test (RRT) and investigate their in vivo antiangiogenic activity through duck ( Anas platyrhynchos ) chorioallantoic membrane (CAM) assay...
2018: Analytical Cellular Pathology (Amsterdam)
Tsung-Jung Tsai, Yun-Ping Lim, Wen-Ying Chao, Chien-Chin Chen, Yi-Ju Chen, Ching-Yen Lin, Ying-Ray Lee
Objective: Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is urgent to develop a biomarker for diagnosis and/or prediction of human CRC. In addition, capping actin protein (CapG) belongs to the gelsolin family and has been reported to contribute on tumor invasion/metastasis in multiple human cancers...
2018: Analytical Cellular Pathology (Amsterdam)
Ivana Miše, Majda Vučić
Syndecan-1 (Sdc1) is a transmembrane heparan sulfate proteoglycan, an extracellular matrix receptor involved in intercellular communication, proliferation, angiogenesis, and metastasis. This study determined and compared Sdc1 expression in the tumor cells and stroma of 30 invasive lobular and 30 invasive ductal breast carcinomas (ILCs/IDCs), also in the axillary node metastases of ductal type, and correlated it with clinical and tumor parameters. Sdc1 was expressed in the epithelium of 90% carcinoma of both histological types...
2018: Analytical Cellular Pathology (Amsterdam)
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