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Enzyme Research

Juan L Rendón, Mauricio Miranda-Leyva, Alberto Guevara-Flores, José de Jesús Martínez-González, Irene Patricia Del Arenal, Oscar Flores-Herrera, Juan P Pardo
A kinetic study of thioredoxin-glutathione reductase (TGR) from Taenia crassiceps metacestode (cysticerci) was carried out. The results obtained from both initial velocity and product inhibition experiments suggest the enzyme follows a two-site ping-pong bi bi kinetic mechanism, in which both substrates and products are bound in rapid equilibrium fashion. The substrate GSSG exerts inhibition at moderate or high concentrations, which is concomitant with the observation of hysteretic-like progress curves. The effect of NADPH on the apparent hysteretic behavior of TGR was also studied...
2018: Enzyme Research
Nor Aliza Abdul Rahim, Marlini Othman, Muna Sabri, David W Stanley
Phospholipase A2 (PLA2 ) is a secretory digestive enzyme that hydrolyzes ester bond at sn-2 position of dietary phospholipids, creating free fatty acid and lysophospholipid. The free fatty acids (arachidonic acid) are absorbed into midgut cells. Aedes albopictus and Culex quinquefasciatus digestive PLA2 was characterized using a microplate PLA2 assay. The enzyme showed substantial activities at 6 and 8  μ g/ μ l of protein concentration with optimal activity at 20 and 25  μ g/ μ l of substrate concentration in Aedes albopictus and Culex quinquefasciatus , respectively...
2018: Enzyme Research
Luana Cunha, Raquel Martarello, Paula Monteiro de Souza, Marcela Medeiros de Freitas, Kleber Vanio Gomes Barros, Edivaldo Ximenes Ferreira Filho, Mauricio Homem-de-Mello, Pérola Oliveira Magalhães
Enzymatic hydrolysis is an important but expensive step in the process to obtain enzyme derived products. Thus, the production of efficient enzymes is of great interest for this biotechnological application. The production of xylanase by Aspergillus foetidus in soybean residues was optimized using 2 × 23 factorial designs. The experimental data was fitted into a polynomial model for xylanase activity. Statistical analyses of the results showed that variables pH and the interaction of pH and temperature had influenced the production of xylanase, with the best xylanase production level (13...
2018: Enzyme Research
Karabi Roy, Sujan Dey, Md Kamal Uddin, Rasel Barua, Md Towhid Hossain
Pectinase is one of the important enzymes of industrial sectors. Presently, most of the pectinases are of plant origin but there are only a few reports on bacterial pectinases. The aim of the present study was to isolate a novel and potential pectinase producing bacterium as well as optimization of its various parameters for maximum enzyme production. A total of forty bacterial isolates were isolated from vegetable dump waste soil using standard plate count methods. Primary screening was done by hydrolysis of pectin...
2018: Enzyme Research
Alex Sander Rodrigues Cangussu, Deborah Aires Almeida, Raimundo Wagner de Souza Aguiar, Sidnei Emilio Bordignon-Junior, Kelvinson Fernandes Viana, Luiz Carlos Bertucci Barbosa, Edson Wagner da Silva Cangussu, Igor Viana Brandi, Augustus Caeser Franke Portella, Gil Rodrigues Dos Santos, Eliane Macedo Sobrinho, William James Nogueira Lima
Phytase plays a prominent role in monogastric animal nutrition due to its ability to improve phytic acid digestion in the gastrointestinal tract, releasing phosphorus and other micronutrients that are important for animal development. Moreover, phytase decreases the amounts of phytic acid and phosphate excreted in feces. Bioinformatics approaches can contribute to the understanding of the catalytic structure of phytase. Analysis of the catalytic structure can reveal enzymatic stability and the polarization and hydrophobicity of amino acids...
2018: Enzyme Research
Umi Baroroh, Muhammad Yusuf, Saadah Diana Rachman, Safri Ishmayana, Mas Rizky A A Syamsunarno, Jutti Levita, Toto Subroto
Starch is a polymeric carbohydrate composed of glucose. As a source of energy, starch can be degraded by various amylolytic enzymes, including α -amylase. In a large-scale industry, starch processing cost is still expensive due to the requirement of high temperature during the gelatinization step. Therefore, α -amylase with raw starch digesting ability could decrease the energy cost by avoiding the high gelatinization temperature. It is known that the carbohydrate-binding module (CBM) and the surface-binding site (SBS) of α -amylase could facilitate the substrate binding to the enzyme's active site to enhance the starch digestion...
2017: Enzyme Research
Doris C Niño-Gómez, Claudia M Rivera-Hoyos, Edwin D Morales-Álvarez, Edgar A Reyes-Montaño, Nury E Vargas-Alejo, Ingrid N Ramírez-Casallas, Kübra Erkan Türkmen, Homero Sáenz-Suárez, José A Sáenz-Moreno, Raúl A Poutou-Piñales, Janneth González-Santos, Azucena Arévalo-Galvis
Phytases are used for feeding monogastric animals, because they hydrolyze phytic acid generating inorganic phosphate. Aspergillus niger 3-phytase A (PDB: 3K4Q) and 3-phytase B (PDB: 1QFX) were characterized using bioinformatic tools. Results showed that both enzymes have highly conserved catalytic pockets, supporting their classification as histidine acid phosphatases. 2D structures consist of 43% alpha-helix, 12% beta-sheet, and 45% others and 38% alpha-helix, 12% beta-sheet, and 50% others, respectively, and pI 4...
2017: Enzyme Research
Oliyad Jeilu Oumer, Dawit Abate
The demand for enzymes in the global market is projected to rise at a fast pace in recent years. There has been a great increase in industrial applications of pectinase owing to their significant biotechnological uses. For applying enzymes at industrial scale primary it is important to know the features of the enzyme. Thus, this study was undertaken with aims of characterizing the pectinase enzyme from Bacillus subtilis strain Btk27 and proving its potential application in demucilisation of coffee. In this study, the maximum pectinase activity was achieved at pH 7...
2017: Enzyme Research
Dennis J Díaz-Rincón, Ivonne Duque, Erika Osorio, Alexander Rodríguez-López, Angela Espejo-Mojica, Claudia M Parra-Giraldo, Raúl A Poutou-Piñales, Carlos J Alméciga-Díaz, Balkys Quevedo-Hidalgo
Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14...
2017: Enzyme Research
Camila Gabriel Kato, Geferson de Almeida Gonçalves, Rosely Aparecida Peralta, Flavio Augusto Vicente Seixas, Anacharis Babeto de Sá-Nakanishi, Lívia Bracht, Jurandir Fernando Comar, Adelar Bracht, Rosane Marina Peralta
The aim of the present study was to compare the in vitro inhibitory effects on the salivary and pancreatic α-amylases and the in vivo hypoglycemic actions of the hydrolysable tannin from Chinese natural gall and the condensed tannin from Acacia mearnsii. The human salivary α-amylase was more strongly inhibited by the hydrolysable than by the condensed tannin, with the concentrations for 50% inhibition (IC50) being 47.0 and 285.4 μM, respectively. The inhibitory capacities of both tannins on the pancreatic α-amylase were also different, with IC50 values being 141...
2017: Enzyme Research
D F Silva, A F A Carvalho, T Y Shinya, G S Mazali, R D Herculano, P Oliva-Neto
The immobilization of cellulases could be an economical alternative for cost reduction of enzyme application. The derivatives obtained in the immobilization derivatives were evaluated in recycles of paper filter hydrolysis. The immobilization process showed that the enzyme recycles were influenced by the shape (drop or sheet) and type of the mixture. The enzyme was recycled 28 times for sheets E' and 13 times for drops B'. The derivative E' showed the highest stability in the recycle obtaining 0.05 FPU/g, RA of 10%, and FPU Yield of 1...
2017: Enzyme Research
Edwin D Morales-Álvarez, Claudia M Rivera-Hoyos, Ángela M Cardozo-Bernal, Raúl A Poutou-Piñales, Aura M Pedroza-Rodríguez, Dennis J Díaz-Rincón, Alexander Rodríguez-López, Carlos J Alméciga-Díaz, Claudia L Cuervo-Patiño
Laccases are multicopper oxidases that catalyze aromatic and nonaromatic compounds with concomitant reduction of molecular oxygen to water. They are of great interest due to their potential biotechnological applications. In this work we statistically improved culture media for recombinant GILCC1 (rGILCC1) laccase production at low scale from Ganoderma lucidum containing the construct pGAPZαA-GlucPost-Stop in Pichia pastoris. Temperature, pH stability, and kinetic parameter characterizations were determined by monitoring concentrate enzyme oxidation at different ABTS substrate concentrations...
2017: Enzyme Research
Bagher Larijani, Ramin Heshmat, Mina Ebrahimi-Rad, Shohreh Khatami, Shirin Valadbeigi, Reza Saghiri
Background and Aims. In the present study, we have investigated the activity of adenosine deaminase (ADA) as a diagnostic marker in type 2 (or II) diabetes mellitus (T2DM). Design and Methods. The deaminase activity of ADA1 and ADA2 was determined in serum from 33 patients with type 2 (or II) diabetes mellitus and 35 healthy controls. We also determined the proportion of glycated hemoglobin (HbA1c). Results. Our results showed significant differences between total serum ADA (tADA) and ADA2 activities in the diabetic groups with HbA1c < 8 (%) and HbA1c ≥ 8 (%) with respect to the values in healthy individuals (p < 0...
2016: Enzyme Research
Ruqayah G Y Al-Obaidi, Bassam M S Al-Musawi, Munib Ahmed K Al-Zubaidi, Christian Oberkanins, Stefan Németh, Yusra G Y Al-Obaidi
Congenital adrenal hyperplasia is a group of autosomal recessive disorders. The most frequent one is 21-hydroxylase deficiency. Analyzing CYP21A2 gene mutations was so far not reported in Iraq. This work aims to analyze the spectrum and frequency of CYP21A2 mutations among Iraqi CAH patients. Sixty-two children were recruited from the Pediatric Endocrine Consultation Clinic, Children Welfare Teaching Hospital, Baghdad, Iraq, from September 2014 till June 2015. Their ages ranged between one day and 15 years...
2016: Enzyme Research
Alex Fernando de Almeida, Kleydiane Braga Dias, Ana Carolina Cerri da Silva, César Rafael Fanchini Terrasan, Sâmia Maria Tauk-Tornisielo, Eleonora Cano Carmona
The aims of this work were to establish improved conditions for lipase production by Candida viswanathii using agroindustrial wastes in solid-state cultivation and to purify and evaluate the application of this enzyme for poultry fat hydrolysis. Mixed wheat bran plus spent barley grain (1 : 1, w/w) supplemented with 25.0% (w/w) olive oil increased the lipase production to 322.4%, compared to the initial conditions. When olive oil was replaced by poultry fat, the highest lipase production found at 40% (w/w) was 31...
2016: Enzyme Research
Supaporn Klangprapan, Ponlatham Chaiyarit, Doosadee Hormdee, Amonrujee Kampichai, Tueanjit Khampitak, Jureerut Daduang, Ratree Tavichakorntrakool, Bhinyo Panijpan, Patcharee Boonsiri
Periodontal diseases, which result from inflammation of tooth supporting tissues, are highly prevalent worldwide. Myeloperoxidase (MPO), from certain white blood cells in saliva, is a biomarker for inflammation. We report our study on the salivary MPO activity and its association with severity of periodontal diseases among Thai patients. Periodontally healthy subjects (n = 11) and gingivitis (n = 32) and periodontitis patients (n = 19) were enrolled. Assessments of clinically periodontal parameters were reported as percentages for gingival bleeding index (GI) and bleeding on probing (BOP), whereas pocket depth (PD) and clinical attachment loss (CAL) were measured in millimeters and then made to index scores...
2016: Enzyme Research
Preety Vatsyayan, Pranab Goswami
A hydrocarbon degrading Aspergillus terreus MTCC 6324 produces a high level of extremely active and stable cellular large catalase (CAT) during growth on n-hexadecane to combat the oxidative stress caused by the hydrocarbon degrading metabolic machinery inside the cell. A 160-fold purification with specific activity of around 66 × 10(5) U mg(-1) protein was achieved. The native protein molecular mass was 368 ± 5 kDa with subunit molecular mass of nearly 90 kDa, which indicates that the native CAT protein is a homotetramer...
2016: Enzyme Research
Gastón Ezequiel Ortiz, Diego Gabriel Noseda, María Clara Ponce Mora, Matías Nicolás Recupero, Martín Blasco, Edgardo Albertó
A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease activity. Biochemical, thermodynamics, and kinetics features such as optimum pH and temperature values, thermal stability, activation energy (E a), quotient energy (Q 10), K m , and V max were studied in four enzymatic extracts from the selected strains that showed the highest productivity...
2016: Enzyme Research
Madison A Smith, Jesica Gonzalez, Anjum Hussain, Rachel N Oldfield, Kathryn A Johnston, Karlo M Lopez
Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus of mature lysyl oxidase. Total enzyme yields were determined to be 1.5 mg for the Nus-A tagged enzyme (0.75 mg/L of media), 7.84 mg for the Trx tagged enzyme (3.92 mg/L of media), and 9.33 mg for the GST tagged enzyme (4...
2016: Enzyme Research
Hemlata Bhosale, Uzma Shaheen, Tukaram Kadam
Hyperthermostable alkaline lipase from Bacillus sonorensis 4R was purified and characterized. The enzyme production was carried out at 80°C and 9.0 pH in glucose-tween inorganic salt broth under static conditions for 96 h. Lipase was purified by anion exchange chromatography by 12.15 fold with a yield of 1.98%. The molecular weight of lipase was found to be 21.87 KDa by SDS-PAGE. The enzyme activity was optimal at 80°C with t 1/2 of 150 min and at 90°C, 100°C, 110°C, and 120°C; the respective values were 121...
2016: Enzyme Research
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