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Journals International Journal of Bioch...

International Journal of Biochemistry and Molecular Biology

https://read.qxmd.com/read/35891642/effect-of-iron-deficiency-anaemia-over-glycated-hemoglobin-in-non-diabetic-women
#21
JOURNAL ARTICLE
D'Sa Janice, Mangalore Balakrishna Prathima, Sushith Sushith, Rachana Narayanan, Shridhar Reshma, Suriyan Nair, Bhuvanesh Sukhlal Kalal
BACKGROUND: Glycated hemoglobin (HbA1c) is a form of hemoglobin bound to glucose and used as an index of glycaemic control reflecting glucose levels of the previous three months. Iron deficiency anemia (IDA) is the commonest form of anemia that affects HbA1c. Reports on the effects of IDA on HbA1c levels are inconsistent in India. Therefore, the study correlated the HbA1c and IDA in non-diabetic female patients. METHODS: A correlative study between HbA1c and IDA was carried out at the Department of Biochemistry, A...
2022: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/35891641/an-improved-method-for-isolation-of-rna-from-rat-femur
#22
JOURNAL ARTICLE
Alka Khera, Thungapathra MuthuKumarappa, Dheeraj Dumir, Poonam Kanta, Gaurav Kumar, Jaswinder Kalra
BACKGROUND: RNA isolation from ossified bone is a difficult and time-consuming process which often results in poor recovery of RNA. The yield is limited and might not be suitable for gene quantification studies by real time PCR. METHODOLOGY: The present study demonstrates RNA extraction from rat femur utilizing the silica column along with the trizol reagent. Quality of RNA was assessed by agarose gel analysis and its suitability for real-time PCR analysis was determined by β-actin Ct values...
2022: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/35611053/effects-of-senegal-haplotype-xmn1-rs7412844-alpha-thalassemia-3-7kb-hba1-hba2-deletion-nprl3-rs11248850-and-bcl11a-rs4671393-variants-on-sickle-cell-nephropathy
#23
JOURNAL ARTICLE
El Hadji Malick Ndour, Khuthala Mnika, Fatou Guèye Tall, Moussa Seck, Indou Dème Ly, Victoria Nembaware, Hélène Ange Thérèse Sagna-Bassène, Rokhaya Dione, Aliou Abdoulaye Ndongo, Jean Pascal Demba Diop, Nènè Oumou Kesso Barry, Moustapha Djité, Rokhaya Ndiaye Diallo, Papa Madièye Guèye, Saliou Diop, Ibrahima Diagne, Aynina Cissé, Ambroise Wonkam, Philomène Lopez Sall
OBJECTIVE: Sickle cell anemia (SCA) can cause substantial kidney dysfunction resulting in sickle cell nephropathy, which may be affected by the presence of modifier genes. This study evaluates the effects of some modifier genes on sickle cell nephropathy. METHODS: Patients living with SCA were recruited. Alpha-thalassemia (3.7kb HBA1/HBA2 deletion) was genotyped using gap PCR multiplex. Senegal haplotype (Xmn1-rs7412844), BCL11A -rs4671393 and NPRL3 -rs11248850 were genotyped using Mass Array...
2022: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/35295914/molnupiravir-favipiravir-and-other-antiviral-drugs-with-proposed-potentials-for-management-of-covid-19-a-concern-on-antioxidant-aspect
#24
JOURNAL ARTICLE
Sora Yasri, Viroj Wiwanitki
COVID-19 is an important global public health problem that causes millions of infections worldwide. The specific antiviral drug for this new infection is still under research. Some new antiviral drugs, including molnupiravir and favipiravir, are proposed for usefulness in management of COVID-19. Additionally, some classic antiviral drugs used for other viral infections are also reproposed for the potentials for management of COVID-19. In the management of COVID-19, there are several pharmacological actions...
2022: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/35103110/the-effect-of-consumption-of-purple-grape-juice-in-the-gestational-period-on-oxidative-stress-parameters-in-wistar-rat-foetuses
#25
JOURNAL ARTICLE
Isabel Cristina Teixeira Proença, Tamires Marques de Abreu, Jessica Pereira Marinho, Malena Rostirola Miri, Gustavo Fernandes Vasques, Luiz Fernando Lopes, Manuela Dos Santos, Vanessa Duarte Ortiz, Patrick Türck, Alex Sander da Rosa Araujo, Claudia Funchal, Daniela Pochmann, Caroline Dani
This study aimed to evaluate the effects of purple grape juice consumption in pregnancy on oxidative stress parameters in Wistar rat fetuses. Twenty-four pregnant rats were divided into five groups: control group, indomethacin group (received a single dose of indomethacin in DG20), group grape juice DG14 (received an amount for 14 days/first and second gestational trim), group grape juice DG20 (received a dose throughout the gestational period), group grape juice two doses (received two doses, at morning and afternoon)...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/35103109/clinical-biomedical-research-of-indoleamine-2-3-dioxygenase-update-on-current-available-reports-from-southeast-asia
#26
REVIEW
Beuy Joob, Sora Yasri, Viroj Wiwanitkit
Indoleamine 2,3-dioxygenase is an important enzyme in human, which plays roles in the rate-limiting first step in tryptophan catabolism. Indoleamine 2,3-dioxygenase is currently studied by research groups worldwide. Association between Indoleamine 2,3-dioxygenase and medial disorders in clinical medicine is proposed and is an interesting topic in clinical biochemical reasearch. Here, the author briefly reviews and discusses on the available important scientific reports on Indoleamine 2,3-dioxygenase from tropical Southeast Asia...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/34084593/pathogens-and-drug-resistance-of-hospital-acquired-pneumonia-in-an-eicu-in-tianjin-china
#27
JOURNAL ARTICLE
Yanan Zhang, Songtao Shou
OBJECTIVE: Observing the pathogens and drug-resistance within hospital-acquired pneumonia (HAP) in an emergency intensive care unit (EICU) to provide a reference for clinically reasonable use of antibiotics. METHODS: Sixty-two patients with HAP in Tianjin Medical University General Hospital from January 2017 to May 2019 were analyzed retrospectively. Bacterial identification and susceptibility were reviewed. RESULTS: One hundred and thirty-seven strains of pathogenic bacteria were isolated from 62 patients, with 97...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/33824778/myp2-locus-genes-sequence-variations-genetic-association-studies-and-haplotypic-association-in-patients-with-high-myopia
#28
JOURNAL ARTICLE
Shabhat Rasool, Rubiya Dar, Mosin S Khan, Sheikh Gazalla Ayoub, Sabia Rashid, Muneeb U Rehman, Tariq Jan, Meenu A Qureshi, Khurshid I Andrabi
High Myopia (HM) is a common complex-trait eye disorder. There is essential evidence that genetic factors play a significant role in the development of nonsyndromic high myopia. Identification of susceptibility genes of high myopia will shed light on the pathophysiological mechanism underlying their genesis. This was a case control study examining the prospect of association of DLGAP1, EMILIN2 & MYOM1 genes on MYP2 locus in purely ethnic (Kashmiri) population representing a homogeneous cohort. Genomic DNA was extracted using phenol chloroform and salting out method...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/33824777/identification-of-a-novel-tpm4-isoform-transcript-and-comparison-to-the-expression-of-other-tropomyosin-isoforms-in-bovine-cardiac-and-skeletal-muscles
#29
JOURNAL ARTICLE
Syamalima Dube, Lynn Abbott, Samender Randhawa, Yingli Fan, Joseph W Sanger, Jean M Sanger, Bernard J Poiesz, Dipak K Dube
In mammals, there are four tropomyosin (TPM) genes (TPM1, TPM2, TPM3, and TPM4) each of which generate a multitude of alternatively spliced mRNAs. TPM isoform diversity in bovine unlike in humans are not well characterized. The purpose of this investigation is to perform an extensive analysis of the expression of both transcripts and corresponding protein of sarcomeric TPMs in bovine strated muscles. We have cloned and sequenced the transcripts of the sarcomeric isoform of the TPM4 gene designated as TPM4α as well as a new splice variant TPM4ε from bovine striated muscles...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/33824776/antioxidant-activity-of-the-simulated-gastro-intestinal-digestion-hydrolysate-of-two-edible-nigerian-marine-molluscs-tympanatonus-fuscatus-var-radula-l-and-pachymelania-aurita-m
#30
JOURNAL ARTICLE
Abraham A Paul, Omolaja R Osoniyi, Queensley A Eghianruwa, Olayemi G Oparinde, Adetunji S Adesina
The multifunctional nature of antioxidant peptides makes them more attractive candidates as dietary ingredients in health maintenance. Therefore, food protein-derived antioxidant peptides are continuously investigated. This study investigated the in vitro antioxidant properties of hydrolysate and ultrafiltered peptide fractions of Pachymelania aurita and Tympanatonus fuscatus var radula-two commonly consumed marine molluscs known as periwinkles in southern Nigeria. Simulated gastrointestinal digestion (SGID) of soluble proteins of T...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/33824775/an-innovative-and-cost-effective-way-to-estimate-alkaline-phosphatase-activity-in-in-vitro-cellular-model-systems
#31
JOURNAL ARTICLE
Poonam Kanta, Tulikalipi Ghosh, Anit Kaur, Thungapathra Muthukumarappa
Alkaline phosphatase is an enzyme that converts para-nitrophenyl phosphate to para-nitrophenol (yellow coloured) in 2-amino, 2-methyl, 1-propanol buffer at pH 10.5. However, when this protocol is applied to the in vitro cellular model systems to estimate alkaline phosphatase activity, it tends to generate clumps of genomic DNA, leading to inaccurate pipetting for protein estimation. The aim of the study was to introduce minor modifications in the existing protocol to make it simple, cost-effective, with minimal labor-intensive procedures while estimating alkaline phosphatase activity in cellular model systems...
2021: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/32211211/novel-antibody-against-tmx2-and-its-effects-on-breast-cancer-cells
#32
JOURNAL ARTICLE
Eleana Hatzidaki, Dimitrios A Ntanovasilis, Ioannis Papasotiriou
Although monoclonal antibodies are promising, a truly fully human antibody is yet to be produced. Current human antibodies have the human sequence, but are produced in either transgenic animals or in phages. The aim of this paper was to produce a truly human antibody directed against an epitope of our choice, secreted by human plasma cells. The target protein was TMX2 one of the least studied disulfide isomerases. IgG and anti-TMX2 antibody were determined by both Elisa and western blot. TMX2 KD was evaluated by Surface Plasmon Resonance...
2020: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/31777682/ccr2-polymorphism-and-hiv-mutation-in-both-mother-and-child-is-associated-with-higher-transmission
#33
JOURNAL ARTICLE
Marie Nicole Ngoufack, Céline N Nkenfou, Barbara A Tiedeu, Georges Nguefack-Tsague, Linda C Mekue Mouafo, Beatrice Dambaya, Carine N Nguefeu, Elvis N Ndzi, Serge C Billong, Wilfred F Mbacham, Alexis Ndjolo
C-C motif chemokine receptor 2 (CCR2) is one of the co-receptors of HIV found on the surface of the target cell and studied as genetic factors known to be associated with HIV infection. This study investigates the influence of mothers' and children's CCR2 polymorphism on HIV acquisition in children. A cross-sectional study was performed in five hospitals in the Northern Region of Cameroon. Blood samples were collected from HIV-infected mothers and their exposed babies. DNA was extracted from the Buffy coat using the QIAamp®DNA mini kit (Qiagen)...
2019: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/31523479/dependence-of-dna-looping-on-escherichia-coli-culture-density
#34
JOURNAL ARTICLE
Justin P Peters, Vishwas N Rao, Nicole A Becker, L James Maher
Repression of a promoter by entrapment within a tightly bent DNA loop is a common mechanism of gene regulation in bacteria. Besides the mechanical properties of the looped DNA and affinity of the protein that anchors the loop, cellular energetics and DNA negative supercoiling are likely factors determining the stability of the repression loop. E. coli cells undergo numerous highly regulated and dynamic transitions as resources are depleted during bacterial growth. We hypothesized that the probability of DNA looping depends on the growth status of the E...
2019: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/31523478/the-ability-of-salts-to-stabilize-proteins-in-vivo-or-intracellularly-correlates-with-the-hofmeister-series-of-ions
#35
JOURNAL ARTICLE
Fatmah M Hani, Ashley E Cole, Elliot Altman
Numerous studies have been conducted on the ability of salts to stabilize proteins in vitro using purified proteins demonstrating the fact that the ability of salts to stabilize proteins correlates with the Hofmeister series of ions. Using the well characterized bacterial aqueous cytosolic β-galactosidase and catechol 2,3-dioxygenase enzymes, we demonstrated that salts can stabilize proteins in vivo or intracellularly as well and that the ability of salts to stabilize these two proteins intracellularly also correlates with the Hofmeister series of ions...
2019: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/31523477/identification-of-calcium-sodium-magnesium-and-chloride-ion-levels-in-hypertensive-and-non-hypertensive-trinidadians
#36
JOURNAL ARTICLE
Andanappa Gadad, Stephanie Mohammed, Venkatesan Sundaram, Shivananda B Nayak, Ashmeed Ryan Mohammed, Terry Gavaskar Ramnanansingh
Hypertension is a progressive cardiovascular disease with association to risk factors. These risk factors in conjunction with essential elements has become identifiable with the development of the progressive disease. The key purpose of this investigation was to identify the levels of the essential elements, Sodium (Na+ ), Magnesium (Mg2+ ), Chloride (Cl- ) and Calcium (Ca2+ ) in both hypertensive and non-hypertensive patients in a Trinidadian population. To achieve this objective a case-control investigation was conducted in which both hypertensive and non-hypertensive patients were assessed...
2019: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/31328020/effect-of-methanolic-crude-extract-of-aframomum-melegueta-a-m-seeds-on-selected-lactogenic-hormones-of-albino-rats
#37
JOURNAL ARTICLE
Hyacinth Ibekwe
The search for alternative control of excessive milk secretion associated with exclusive breast feeding necessitated this study. This study was designed to investigate the effect of methanolic crude extract of Aframomum melegueta (A.m) seed by measuring serum concentrations of selected lactogenic (prolactin, estradiol and progesterone) hormones in lactating and non-lactating Albino rats. A total of 18 non-lactating and lactating rats each were assigned into three (3) treatments of 6 rats each. Treatment 1 and 2 (controls) received 0...
2019: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/31149366/in-vitro-antiproliferative-studies-of-extracts-of-the-marine-molluscs-tympanatonus-fuscatus-var-radula-linnaeus-and-pachymelania-aurita-muller
#38
JOURNAL ARTICLE
Queensley Eghianruwa, Omolaja Osoniyi, Sabina Wachira, Naomi Maina, Regina Mbugua, Mabel Imbuga
This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; T.fuscatus and P.aurita , against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines in vitro . The antimitotic activity of the extracts was evaluated using Allium cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6)...
2019: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/30515347/the-dangers-of-sublethal-carvacrol-exposure-increases-in-virulence-of-bacillus-cereus-during-endophthalmitis
#39
JOURNAL ARTICLE
Niloofar Rajabli, Lauren Williamson, Pierre S Nimmer, Marie Kelly-Worden, Jill S Bange, Yenling Ho, John L McKillip
Bacillus cereus can cause endophthalmitis through secretion of virulence factors, including hemolysin BL (Hbl) and nonhemolytic entertoxin (Nhe). Carvacrol is an extract from oregano oil, with potential for curtailing B. cereus endophthalmitis, due to antimicrobial and anti-inflammatory qualities. However, sublethal levels of carvacrol increases B. cereus virulence. The goal of this study was to investigate the increase in B. cereus virulence potential in response stress induced by a subinhibitory concentration (SIC) of carvacrol...
2018: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/29531845/expression-of-reca-and-cell-penetrating-peptide-cpp-fusion-protein-in-bacteria-and-in-mammalian-cells
#40
JOURNAL ARTICLE
Xiubao Chang, Yuexian Hou
Genome editing is a powerful tool to modify a specific gene and to correct a disease-causing mutation. Recently developed new techniques, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9), significantly facilitate the progression in this field. However, mutations associated with the double strand DNA breaks (DSBs) introduced by these systems hampered their direct usage in clinic. In order to prevent the mutations caused by DSBs, we have designed a novel mean to induce homology-directed recombination (HDR) without DSBs, i...
2018: International Journal of Biochemistry and Molecular Biology
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