journal
https://read.qxmd.com/read/37902166/primed-inflammatory-response-by-fibroblast-subset-is-necessary-for-proper-oral-and-cutaneous-wound-healing
#21
JOURNAL ARTICLE
Zhaoxu Chen, Rahul Debnath, Ifeoma Chikelu, Jonathan X Zhou, Kang I Ko
Fibroblasts are ubiquitous mesenchymal cells that exhibit considerable molecular and functional heterogeneity. Besides maintaining stromal integrity, oral fibroblast subsets are thought to play an important role in host-microbe interaction during injury repair, which is not well explored in vivo. Here, we characterize a subset of fibroblast lineage labeled by paired-related homeobox-1 promoter activity (Prx1Cre+ ) in oral mucosa and skin and demonstrate these fibroblasts readily respond to microbial products to facilitate the normal wound healing process...
October 30, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37880921/enhanced-transformation-efficiency-in-treponema-denticola-enabled-by-syngenicdna-based-plasmids-lacking-restriction-modification-target-motifs
#22
JOURNAL ARTICLE
Christopher D Johnston, M Paula Goetting-Minesky, Kelly Kennedy, Valentina Godovikova, Sara M Zayed, Richard J Roberts, J Christopher Fenno
Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of tissue homeostatic processes that leads to breakdown of the tissue and bone supporting the teeth in periodontal disease. Additionally, our group has recently demonstrated that Treponema are among the dominant microbial genera detected intracellularly in tumor specimens from patients with oral squamous cell carcinoma. While over 60 species and phylotypes of oral Treponema have been detected, T. denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is regularly performed...
October 25, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37850509/metabolism-of-serine-glycine-lipids-by-human-gingival-cells-in-culture
#23
JOURNAL ARTICLE
Tyler M Guido, Samuel D Ratcliffe, Amanda Rahmlow, Matthew A Zambrello, Anthony A Provates, Robert B Clark, Michael B Smith, Frank C Nichols
Porphyromonas gingivalis produces five classes of serine/glycine lipids that are recovered in lipid extracts from periodontitis-afflicted teeth and diseased gingival tissues, particularly at sites of periodontitis. Because these lipids are recovered in diseased gingival tissues, the purpose of the present study was to evaluate the capacity of cultured human gingival fibroblasts (HGF), keratinocytes, and macrophages to hydrolyze these lipids. We hypothesize that one or more of these cell types will hydrolyze the serine/glycine lipids...
October 18, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37804481/critical-review-of-16s-rrna-gene-sequencing-workflow-in-microbiome-studies-from-primer-selection-to-advanced-data-analysis
#24
REVIEW
Alba Regueira-Iglesias, Carlos Balsa-Castro, Triana Blanco-Pintos, Inmaculada Tomás
The multi-batch reanalysis approach of jointly reevaluating gene/genome sequences from different works has gained particular relevance in the literature in recent years. The large amount of 16S ribosomal ribonucleic acid (rRNA) gene sequence data stored in public repositories and information in taxonomic databases of the same gene far exceeds that related to complete genomes. This review is intended to guide researchers new to studying microbiota, particularly the oral microbiota, using 16S rRNA gene sequencing and those who want to expand and update their knowledge to optimise their decision-making and improve their research results...
October 7, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37801007/histone-acetylation-bet-proteins-and-periodontal-inflammation
#25
REVIEW
Nicholas Clayton, David Pellei, Zhao Lin
Periodontitis is one of the most common inflammatory diseases in humans. The susceptibility to periodontitis is largely determined by the host response, and the severity of inflammation predicts disease progression. Upon microbial insults, host cells undergo massive changes in their transcription program to trigger an appropriate response (inflammation). It is not surprising that successful keystone pathogens have developed specific mechanisms to manipulate the gene expression network in host cells. Emerging data has indicated that epigenetic regulation plays a significant role in inflammation...
October 6, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37786286/dying-for-a-cause-the-pathogenic-manipulation-of-cell-death-and-efferocytic-pathways
#26
REVIEW
Kelley N Cooper, Jan Potempa, Juhi Bagaitkar
Cell death is a natural consequence of infection. However, although the induction of cell death was solely thought to benefit the pathogen, compelling data now show that the activation of cell death pathways serves as a nuanced antimicrobial strategy that couples pathogen elimination with the generation of inflammatory cytokines and the priming of innate and adaptive cellular immunity. Following cell death, the phagocytic uptake of the infected dead cell by antigen-presenting cells and the subsequent lysosomal fusion of the apoptotic body containing the pathogen serve as an important antimicrobial mechanism that furthers the development of downstream adaptive immune responses...
October 2, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37767604/oral-microbial-biomap-in-the-drought-environment-sjogren-s-syndrome
#27
REVIEW
Laura Bustos-Lobato, Maria J Rus, Carlos Saúco, Aurea Simon-Soro
Sjogren's syndrome (SS) is an autoimmune disease that affects primarily the salivary glands, making perturbations in the oral ecosystem and potential factors of salivary flow that influence the onset and development of the disease. The oral cavity contains diverse microorganisms that inhabit various niches such as the oral microbial "biomap." It does not seem specific enough to establish a characteristic microbiome, given the diversity of clinical manifestations, variable rates of salivary secretion, and influential risk factors in patients with SS...
September 28, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37750230/exploring-the-genetic-and-functional-diversity-of-porphyromonas-gingivalis-long-fimbriae
#28
JOURNAL ARTICLE
Hendrik Leonhard Meyer, Mohamed M H Abdelbary, Eva Miriam Buhl, Christoph Kuppe, Georg Conrads
Porphyromonas gingivalis is a key pathobiont in periodontitis. Its long fimbriae consist of a single anchor (FimB), a varying number of stalk (FimA), and three accessory (tip-related) proteins (FimC, FimD, and FimE). Based on 133 strains/genomes available, it was our aim to investigate the diversity within FimA and FimB and explain the variety of long fimbriae (super-)structures. Combining the new forward primer fimAnewF with the established fimAunivR, we were able to amplify and sequence fimA including its leader region covering all genotypes and serotypes for phylogenetic analysis...
September 26, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37718989/requirements-for-anti-aquaporin-5-autoantibody-production-in-a-mouse-model
#29
JOURNAL ARTICLE
Sabin Acharya, Ahreum Lee, Hyunjin Kim, Hyeong-Jin Kim, Youngnim Choi
Several oral bacteria, including Prevotella melaninogenica (Pm), have aquaporin (AQP) proteins homologous to human AQP5, a major water channel protein targeted in Sjogren's syndrome. This study aimed to understand the antigenic characteristics that induce autoantibodies against an AQP5 "E" epitope (AQP5E) in a mouse model using C57BL/6 mice. Immunization with a PmE-L peptide derived from Pm AQP, which contains amino acid mismatches both at the B- and T-cell epitopes, efficiently induced anti-AQP5E autoantibodies accompanied by increased germinal center (GC) B and follicular helper T cells in the draining lymph nodes...
September 18, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37718958/fusobacterium-nucleatum-triggers-senescence-phenotype-in-gingival-epithelial-cells
#30
JOURNAL ARTICLE
Emmanuel Albuquerque-Souza, Benjamin Shelling, Min Jiang, Xia-Juan Xia, Kantapon Rattanaprukskul, Sinem Esra Sahingur
The prevalence of periodontitis increases with physiological aging. However, whether bacteria associated with periodontal diseases foster aging and the mechanisms by which they may do so are unknown. Herein, we hypothesize that Fusobacterium nucleatum, a microorganism associated with periodontitis and several other age-related disorders, triggers senescence, a chief hallmark of aging responsible to reduce tissue repair capacity. Our study analyzed the senescence response of gingival epithelial cells and their reparative capacity upon long-term exposure to F...
September 18, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37715517/a-novel-sucnr1-inhibitor-alleviates-dysbiosis-through-inhibition-of-host-responses-without-direct-interaction-with-host-microbiota
#31
JOURNAL ARTICLE
Scott C Thomas, Yuqi Guo, Fangxi Xu, Deepak Saxena, Xin Li
Type 2 diabetes (T2D) is a chronic metabolic disorder in which insulin resistance and impaired insulin secretion result in altered metabolite balance, specifically elevated levels of circulating glucose and succinate, which increases the risk of many pathologies, including periodontitis. Succinate, a tricarboxylic acid (TCA) cycle intermediate, can be produced and metabolized by both host cells and host microbiota, where elevated levels serve as an inflammation and pathogen threat signal through activating the succinate G protein-coupled receptor, SUCNR1...
September 16, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37014754/human-papillomavirus-and-epstein-barr-virus-co-infection-in-oral-and-oropharyngeal-squamous-cell-carcinomas-a-systematic-review-and-meta-analysis
#32
REVIEW
Rifat Rahman, Mushfiq H Shaikh, Divya Gopinath, Adi Idris, Newell W Johnson
Squamous cell carcinoma of the oral cavity (OSCC) is the most common head-and-neck malignancy. Importantly, we are experiencing an alarming rise in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) globally. Oncogenic viruses, human papillomavirus (HPV) and Epstein-Barr virus (EBV), are known to be co-associated with OSCC and OPSCC cases. However, the reported incidence of HPV and EBV co-infection in OSCCs and OPSCCs globally is unknown. To address this, we performed a formal meta-analysis and systematic review on published studies that report the detection of both EBV and HPV in OSCCs and OPSCCs...
August 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37459655/tannerella-forsythia-scavenges-fusobacterium-nucleatum-secreted-nod2-stimulatory-molecules-to-dampen-oral-epithelial-cell-inflammatory-response
#33
JOURNAL ARTICLE
Rajendra P Settem, Angela Ruscitto, Sreedevi Chinthamani, Kiyonobu Honma, Ashu Sharma
The oral organism Tannerella forsythia is auxotrophic for peptidoglycan amino sugar N-acetylmuramic acid (MurNAc). It survives in the oral cavity by scavenging MurNAc- and MurNAc-linked peptidoglycan fragments (muropeptides) secreted by co-habiting bacteria such as Fusobacterium nucleatum with which it forms synergistic biofilms. Muropeptides, MurNAc-l-Ala-d-isoGln (MDP, muramyl dipeptide) and d-γ-glutamyl-meso-DAP (iE-DAP dipeptide), are strong immunostimulatory molecules that activate nucleotide oligomerization domain (NOD)-like innate immune receptors and induce the expression of inflammatory cytokines and antimicrobial peptides...
July 17, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37440366/gene-expression-and-metabolic-activity-of-streptococcus-mutans-during-exposure-to-dietary-carbohydrates-glucose-sucrose-lactose-and-xylitol
#34
JOURNAL ARTICLE
Veronika Jurakova, Veronika Farková, Jiri Kucera, Katerina Dadakova, Martina Zapletalova, Katerina Paskova, Roman Reminek, Zdenek Glatz, Lydie Izakovicova Holla, Filip Ruzicka, Jan Lochman, Petra Borilova Linhartova
Recent RNA sequencing studies have given us a deeper insight into the cariogenic impact of carbohydrate sources in the bacterium Streptococcus mutans, the principal microbial agent in dental caries etiopathogenesis. The process of dental caries development is facilitated by the ability of this bacterium to ferment some carbohydrates into organic acids contributing to a pH decrease in the oral cavity and the demineralization of the hard tissues of the tooth. Furthermore, in dental caries progression, biofilm formation, which starts and ends with free planktonic cells, plays an important role and has several unique properties called virulence factors...
July 13, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37347653/accessory-fimbrial-subunits-and-ppad-are-necessary-for-tlr2-activation-by-porphyromonas-gingivalis
#35
JOURNAL ARTICLE
Aleksandra Wielento, Grzegorz P Bereta, Katarzyna Szczęśniak, Anna Jacuła, Marina Terekhova, Maxim N Artyomov, Yoshiaki Hasegawa, Aleksander M Grabiec, Jan Potempa
Porphyromonas gingivalis is an oral pathogen that promotes dysbiosis by quenching the bactericidal activity of the host immune system while maintaining chronic inflammation, leading to periodontitis. This involves the secretion of virulence factors such as P. gingivalis peptidyl arginine deiminase (PPAD), which converts the C-terminal Arg residues of bacterial and host-derived proteins and peptides into citrulline. We have previously shown that PPAD activity and major fimbriae (containing FimA) are necessary for P...
June 22, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37347649/loss-of-signal-transducer-and-activator-of-transcription-3-in-osteoblasts-impaired-the-bone-healing-in-inflammatory-microenvironment
#36
JOURNAL ARTICLE
Jingyi Feng, Zijing Huang, Jiarui Lu, Laiting Chan, Xin Feng, Lizhen Lei, Zhuwei Huang, Lichieh Lin, Yichen Yao, Xiaolei Zhang
INTRODUCTION: This study aimed to investigate the effect of Stat3 on the osteoblast-mediated bone healing in the inflammatory lesion. METHODS: The conditional knockout of Stat3 in osteoblasts (Stat3 CKO) was generated via the Cre-loxP recombination system using Osterix-Cre transgenic mice. The calvarial bone inflammatory lesions were established on both Stat3 CKO and wild-type mice, then harvested to assess the bone healing. In response to lipopolysaccharide (LPS) stimulation, osteoblasts from Stat3 CKO and wild-type mice were subjected to examine the formation of calcium deposits, the expression of osteogenic markers (i...
June 22, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37339018/a-conditional-gene-expression-system-in-porphyromonas-gingivalis-for-study-of-the-secretion-mechanisms-of-lipoproteins-and-t9ss-cargo-proteins
#37
JOURNAL ARTICLE
Yuko Sasaki, Mikio Shoji, Takayuki Sueyoshi, Satoshi Shibata, Takehiro Matsuo, Hideharu Yukitake, Matthias Wolf, Mariko Naito
The Gram-negative anaerobe, Porphyromonas gingivalis, is known to be a pathogen associated with chronic periodontitis. P. gingivalis possesses virulence factors such as fimbriae and gingipain proteinases. Fimbrial proteins are secreted to the cell surface as lipoproteins. In contrast, gingipain proteinases are secreted into the bacterial cell surface via the type IX secretion system (T9SS). The transport mechanisms of lipoproteins and T9SS cargo proteins are entirely different and remain unknown. Therefore, using the Tet-on system developed for the genus Bacteroides, we newly created a conditional gene expression system in P...
June 20, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37257865/functional-profile-of-oral-plaque-microbiome-further-insight-into-the-bidirectional-relationship-between-type-2-diabetes-and-periodontitis
#38
JOURNAL ARTICLE
Nicoletta Favale, Roberto Farina, Alberto Carrieri, Anna Simonelli, Mattia Severi, Silvia Sabbioni, Leonardo Trombelli, Chiara Scapoli
Increasing evidence support the association between the oral microbiome and human systemic diseases. This association may be attributed to the ability of many oral microbes to influence the inflammatory microenvironment. Herein, we focused our attention on the bidirectional relationship between periodontitis and type 2 diabetes using high-resolution whole metagenomic shotgun analysis to explore the composition and functional profile of the subgingival microbiome in diabetics and non-diabetics subjects with different periodontal conditions...
May 31, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37224274/one-carbon-metabolism-and-microbial-pathogenicity
#39
REVIEW
Kendall S Stocke, Richard J Lamont
One-carbon metabolism (OCM) pathways are responsible for several functions, producing a number of one-carbon unit intermediates (formyl, methylene, methenyl, methyl) that are required for the synthesis of various amino acids and other biomolecules such as purines, thymidylate, redox regulators, and, in most microbes, folate. As humans must acquire folate from the diet, folate production is a target for antimicrobials such as sulfonamides. OCM impacts the regulation of microbial virulence such that in a number of instances, limiting the availability of para-aminobenzoic acid (pABA), an essential OCM precursor, causes a reduction in pathogenicity...
May 24, 2023: Molecular Oral Microbiology
https://read.qxmd.com/read/37216657/mir-27a-5p-alleviates-periodontal-inflammation-by-targeting-phosphatase-and-tensin-homolog-deleted-on-chromosome-ten
#40
JOURNAL ARTICLE
Li Deng, Peng-Cheng Huo, Mei-Ting Feng, Rui-Ling Wang, Rui Jing, Li-Jun Luo
INTRODUCTION: MicroRNAs (miRNAs), a type of non-coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR-27a-5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR-27a-5p affects the pathogenesis of periodontitis and its associated biological functions. METHODS: Quantitative real-time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR-27a-5p transcription...
May 22, 2023: Molecular Oral Microbiology
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