Journal of Investigative and Clinical Dentistry

AIMS: The aim of the present study was to determine the range, frequency, prevalence, and distribution of oral lesions submitted for histopathological diagnosis at the oral pathology laboratory at Kuwait University. METHODS: A retrospective analysis was conducted of all cases submitted to the oral pathology laboratory over an 18-year period. Age, sex, histopathological diagnosis, and location of the lesions were recorded. Lesions were classified into 10 diagnostic categories...

AIM: The aim of the present study was to compare patient morbidity and healing outcomes of free gingival graft (FGG) harvesting at palatal donor sites and after using platelet-rich fibrin (PRF) and oxidized regenerated cellulose (ORC). FGG harvesting leaves open palatal wounds, which heal by secondary intention. Patients might experience discomfort or pain during the first 2 weeks of healing. METHODS: Eighteen participants requiring two FGG were recruited. The test and control sites were covered with PRF membrane and ORC...

The aim of the present study was to compare the restorative time for filling cavities in posterior teeth using bulk-fill and conventional resin composites through a systematic review and meta-analysis. A search for clinical trials and laboratory studies was performed in PubMed/MEDLINE, Scopus, the Latin American and Caribbean Health Sciences Literature database, the Brazilian Library in Dentistry, Cochrane Library, Clinical Trials, and ReBEC (Registro Brasileiro de Ensaios Clínicos) databases without publication year or language restriction...

AIM: To determine whether caries risk factors, including cariogenic bacterial levels and salivary function, can be used to identify orthodontic patients who develop white spot lesions (WSL). METHODS: This prospective case-control study comprised 50 patients 11-17 years of age, including 25 controls and 25 cases who developed new WSL during treatment. WSL, oral hygiene and fluorosis were evaluated from intraoral photographs. The biofilm was assessed with bacterial cultures and adenosine triphosphate (ATP) bioluminescence...

AIM: The aim of the present study was to evaluate the pushout bond strength between glass-fiber posts to different regions of intraradicular dentin with different materials for adhesive cementation. METHODS: After endodontic filling, 40 teeth were divided into five groups according to adhesive cementation: Adper Single Bond 2 + RelyX ARC, Excite DSC + RelyX ARC (EXC), Adper SE Plus + RelyX ARC (SEP), RelyX Unicem, and Set. After the bonding process, roots were sectioned obtaining slices to be analyzed from each third...

AIM: Physical properties of obturating materials have a direct effect on the quality of obturation; less viscous material will tend to extrude beyond the apex. We hypothesize that the use of absorbable gelatin sponge (AGS) can prevent the extrusion of these materials. This study aimed to evaluate the quality of obturation in root canals filled with iodoform-calcium hydroxide (Metapex) premixed paste and Metapex with AGS (MAGS) in primary molars. METHODS: This in vivo study comprised 60 primary mandibular molars which after instrumentation were divided into 2 groups according to the obturation material used, Metapex and MAGS, followed by radiographic evaluation to assess the quality of the obturation...

AIM: To outline the current literature surrounding natal teeth, and then, in a pilot study, to evaluate natal teeth using micro-computed tomography (micro-CT) to determine their anatomical profile, and compare and contrast different analytical methods to assess natal teeth. METHODS: 2 extracted natal teeth (mandibular central incisors) and 1 exfoliated mandibular primary central incisor were subjected to micro-CT analysis. RESULTS: Within natal teeth, there were no statistical differences in tooth mineral density (TMD) of both enamel and dentine (P > ...

AIM: Oral malodor is a common condition caused by some Gram-negative oral bacteria, among which are the 3 red complex bacteria (RCB). The present study investigated the effectiveness of the Ultrasound Tongue Scraper (UTS) to disrupt the structural morphology of the bacteria and their biofilm. METHODS: While developing over 72 hours, multispecies biofilms of RCB (Porphromonas gingivalis, Tryponema denticola, Tannerella forsythia) were treated every 24 hours with 1...

AIM: To study the expression of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and fibroblast growth factor-inducible immediate early response protein 14 (Fn14) in oral squamous cell carcinoma (OSCC), to elucidate the possible role of TWEAK-Fn14 in OSCC development. METHODS: Immunohistochemistry for TWEAK-Fn14 was performed on 61 oral mucosal samples: healthy oral mucosa (HOM; N = 15); oral dysplastic lesions (ODL; N = 15); and OSCC (N = 31)...

AIM: This study aimed to investigate the adhesion of Streptococcus sanguinis to the surface of interim prostheses that were treated or not treated with a light-activated glaze, and subjected or not subjected to a thermocycling procedure. METHODS: 36 specimens of each resin were divided into 4 groups: heat-activated acrylic resin; chemically-activated acrylic resin; bis-acryl composite resin (Protemp; 3M ESPE); and bis-GMA (Charisma; Heraeus Kulzer). Half of the specimens underwent application of glaze and the other half underwent mechanical polishing...

To seek scientific evidence to support the effectiveness of invisible aligners, in the aesthetic and functional aspects, compared with that of conventional braces. An electronic search was performed with a complementary grey literature search for in vivo research. No language restrictions were applied. Scopus, PubMed, Web of Science, Cochrane, ClinicalTrials and GreyLiterature databases were used. Studies were first selected by title and abstract; those potentially eligible were read in full. Non-randomized studies were assessed for risk of bias using the tools Methodological Index for Non-randomized Studies (MINORS) and Cochrane Collaboration Common Scheme for Bias as a function of the presence of randomization...

AIM: Oral carcinogenesis cascade is a complex process, characterized by variable numbers of genetic and epigenetic alterations of various genes with manifold roles that could serve as biological hallmarks. This study was undertaken to assess the protein expression of SOX2 and podoplanin in oral epithelial dysplasia and correlate the expression with clinicopathological parameters and risk of malignant transformation. METHODS: SOX2 and podoplanin expression were analyzed in 60 cases of oral epithelial dysplasia...

AIM: Platelet-rich fibrin (PRF) matrices are compared with regard to their ability to retain and release growth factors. Although this ability is thought to influence regenerative outcomes, it remains unclear how it is regulated. To address this question, we compared advanced PRF (A-PRF) and concentrated growth factor (CGF) matrices in terms of distribution of platelets, transforming growth factor-β1, platelet-derived growth factor-BB, vascular endothelial growth factor and matrix metalloprotease-9 (MMP9)...

Lichen planus is a common chronic, inflammatory, immune-mediated mucocutaneous disorder affecting the skin and mucosa. The role of mast cells in the genesis of lichen planus has been debated. Establishing a definitive part played by mast cells and its degranulation would possibly provide a permanent, cost-effective treatment modality for oral lichen planus (OLP). This review aims to study the expression of mast cells qualitatively and quantitatively in OLP. The research questions were framed to assess the mast cell count, localization within the epithelium basement membrane zone and degranulation of mast cells...

AIM: To evaluate the prevalence of oral lesions (OL) and associated factors among survivors of breast cancer (BC). METHODS: A cross-sectional study involving 150 BC survivors was conducted at a public hospital in southern Brazil. Data were collected on socioeconomic aspects, treatment characteristics and oral problems. The decayed, missing and filled teeth index and the occurrence of OL were evaluated. Logistic regression was performed to determine independent variables associated with the outcome...

AIM: The aim of this study was to determine the impact of different full-mouth decontamination (FMD) protocols on the effectiveness of an intrapocket anesthetic gel in periodontal maintenance patients. METHODS: Patients undergoing the periodontal maintenance program and with the need for FMD participated in this study. Patients were randomly allocated to non-surgical periodontal therapy (NSPT) with either a preparatory 15-day decontamination phase, including chlorhexidine mouth rinse and domiciliary hygiene instructions (modified FMD: test group), or without it (FMD: control group)...

AIM: To compare the shear bond strengths of a calcium silicate-based root canal sealer (CSS), to dentin or calcium silicate-impregnated gutta-percha (CSGP), with the shear bond strengths of an epoxy resin-based sealer. METHODS: For sealer/dentin testing, 40 hemisectioned roots were divided into 2 groups. Coronal root dentin discs were bonded with CSS or epoxy resin-based sealer. For sealer/core material testing, CSGP and conventional gutta-percha discs were bonded with a matching sealer (N = 20 per group)...

AIM: To evaluate the effectiveness of topical insulin-liposomal gel in aphthous ulcer treatment. METHODS: 80 participants with minor aphthous ulcers were randomly divided to receive either topical insulin-liposomal gel or placebo gel (once daily) for 6 days. Assessment of outcomes included visual analog scale (VAS) for pain (primary outcome), and secondary outcomes included ulcer duration and impact of treatment on quality of life using the Oral Health Impact Profile 14 (OHIP-14)...

AIM: The aim of the present study was to delineate the epidemiological profile of young patients with squamous cell carcinoma (SCC) treated at a healthcare service in northeastern Brazil. METHODS: A cross-sectional study was performed involving the quantitative analysis of 51 medical records from patients diagnosed with SCC between 1998 and 2013. All patients aged ≤50 years were included in the study. Statistical analysis involved Pearson's χ2 -test, Fisher's exact test, and the Mann-Whitney U-test, with the level of significance set at 5% (P < ...

AIM: Oral squamous cell carcinoma (OSCC) is the most frequently occurring cancer among head and neck SCC worldwide. The identification of novel effective biomarkers for early detection may greatly improve the survival rate and prognosis of patients with OSCC. This study aimed to identify specific oral microbial profiles associated with OSCC. METHODS: Saliva samples were collected from oral leukoplakia (OLK) and OSCC patients (N = 6 each) and healthy controls (HC; N = 4)...