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JOURNAL ARTICLE
Joanna Tannous, Casey M Cosetta, Milton T Drott, Tomás A Rush, Paul E Abraham, Richard J Giannone, Nancy P Keller, Benjamin E Wolfe
Potent antimicrobial metabolites are produced by filamentous fungi in pure culture, but their ecological functions in nature are often unknown. Using an antibacterial Penicillium isolate and a cheese rind microbial community, we demonstrate that a fungal specialized metabolite can regulate the diversity of bacterial communities. Inactivation of the global regulator, LaeA, resulted in the loss of antibacterial activity in the Penicillium isolate. Cheese rind bacterial communities assembled with the laeA deletion strain had significantly higher bacterial abundances than the wild-type strain...
May 10, 2023: MBio
#22
JOURNAL ARTICLE
Lilian Göttig, Christina Weiß, Miona Stubbe, Lisa Hanrieder, Samuel Hofmann, Alessandro Grodziecki, Daniela Stadler, Arnaud Carpentier, Ulrike Protzer, Sabrina Schreiner
Apobec3A is involved in the antiviral host defense, targeting nuclear DNA, introducing point mutations, and thereby activating DNA damage response (DDR). Here, we found a significant upregulation of Apobec3A during HAdV infection, including Apobec3A protein stabilization mediated by the viral proteins E1B-55K and E4orf6, which subsequently limited HAdV replication and most likely involved a deaminase-dependent mechanism. The transient silencing of Apobec3A enhanced adenoviral replication. HAdV triggered Apobec3A dimer formation and enhanced activity to repress the virus...
May 8, 2023: MBio
#23
JOURNAL ARTICLE
Bhavya Papudeshi, Douglas B Rusch, David VanInsberghe, Curtis M Lively, Robert A Edwards, Farrah Bashey
To what extent are generalist species cohesive evolutionary units rather than a compilation of recently diverged lineages? We examine this question in the context of host specificity and geographic structure in the insect pathogen and nematode mutualist Xenorhabdus bovienii. This bacterial species partners with multiple nematode species across two clades in the genus Steinernema. We sequenced the genomes of 42 X. bovienii strains isolated from four different nematode species and three field sites within a 240-km2 region and compared them to globally available reference genomes...
May 8, 2023: MBio
#24
JOURNAL ARTICLE
Olga G Ovchinnikova, Logan P Treat, Tanisha Teelucksingh, Bradley R Clarke, Taryn A Miner, Chris Whitfield, Kimberly A Walker, Virginia L Miller
Klebsiella pneumoniae is a leading cause of nosocomial infections, including pneumonia, bacteremia, and urinary tract infections. Treatment options are increasingly restricted by the high prevalence of resistance to frontline antibiotics, including carbapenems, and the recently identified plasmid-conferred colistin resistance. The classical pathotype (cKp) is responsible for most of the nosocomial infections observed globally, and these isolates are often multidrug resistant. The hypervirulent pathotype (hvKp) is a primary pathogen capable of causing community-acquired infections in immunocompetent hosts...
May 4, 2023: MBio
#25
JOURNAL ARTICLE
Jianwei Zhang, Youzhi Feng, Meng Wu, Ruirui Chen, Zhongpei Li, Xiangui Lin, Yongguan Zhu, Manuel Delgado-Baquerizo
No abstract text is available yet for this article.
May 3, 2023: MBio
#26
JOURNAL ARTICLE
A Sporulation-Independent Way of Life for Bacillus thuringiensis in the Late Stages of an Infection.
Hasna Toukabri, Didier Lereclus, Leyla Slamti
The formation of endospores has been considered the unique survival and transmission mode of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial form. However, nonsporulated bacteria (Spo- ) were reported at the early stages following the death of a host infected with Bacillus thuringiensis, an entomopathogenic sporulating bacterium. Here, we investigated the characteristics of the bacterial population in the late stages of an infection in the B. thuringiensis/Galleria mellonella infection model...
April 27, 2023: MBio
#27
JOURNAL ARTICLE
Andrew D Farr, Diego Pesce, Suman G Das, Mark P Zwart, J Arjan G M de Visser
Adaptive evolutionary processes are constrained by the availability of mutations which cause a fitness benefit and together make up the fitness landscape, which maps genotype space onto fitness under specified conditions. Experimentally derived fitness landscapes have demonstrated a predictability to evolution by identifying limited "mutational routes" that evolution by natural selection may take between low and high-fitness genotypes. However, such studies often utilize indirect measures to determine fitness...
April 27, 2023: MBio
#28
JOURNAL ARTICLE
James F Colbert, Joshua M Kirsch, Christopher L Erzen, Christophe J Langouët-Astrié, Grace E Thompson, Sarah A McMurtry, Jennifer M Kofonow, Charles E Robertson, Elizabeth J Kovacs, Ryan C Sullivan, Joseph A Hippensteel, Namrata V Sawant, Nicole J De Nisco, Bruce D McCollister, Robert S Schwartz, Alexander R Horswill, Daniel N Frank, Breck A Duerkop, Eric P Schmidt
Prior research has focused on host factors as mediators of exaggerated sepsis-associated morbidity and mortality in older adults. This focus on the host, however, has failed to identify therapies that improve sepsis outcomes in the elderly. We hypothesized that the increased susceptibility of the aging population to sepsis is not only a function of the host but also reflects longevity-associated changes in the virulence of gut pathobionts. We utilized two complementary models of gut microbiota-induced experimental sepsis to establish the aged gut microbiome as a key pathophysiologic driver of heightened disease severity...
April 27, 2023: MBio
#29
JOURNAL ARTICLE
Anamika Yadav, Peeyush Jain, Kusum Jain, Yue Wang, Aditi Singh, Ashutosh Singh, Jianping Xu, Anuradha Chowdhary
Lodderomyces elongisporus is a recently emerging yeast pathogen predominantly reported in adult patients who had immunosuppression and/or intravenous access devices. Here, we report a fungemia outbreak caused by L. elongisporus in a neonatal intensive care unit (NICU) in Delhi, India, from September 2021 to February 2022. All 10 neonates had low birthweight, and nine of the patients survived after amphotericin B treatment. Whole-genome sequence analyses of the patient isolates as well as those from other sources in India grouped them into two clusters: one cluster consists of isolates exclusively from stored apples and the other cluster includes isolates from patients, clinical environments, and stored apples...
April 27, 2023: MBio
#30
JOURNAL ARTICLE
Hasan Tükenmez, Pardeep Singh, Souvik Sarkar, Melike Çakır, Ana H Oliveira, Cecilia Lindgren, Karolis Vaitkevicius, Mari Bonde, A Elisabeth Sauer-Eriksson, Fredrik Almqvist, Jörgen Johansson
Listeria monocytogenes is a facultative Gram-positive bacterium that causes listeriosis, a severe foodborne disease. We previously discovered that ring-fused 2-pyridone compounds can decrease virulence factor expression in Listeria by binding and inactivating the PrfA virulence activator. In this study, we tested PS900, a highly substituted 2-pyridone that was recently discovered to be bactericidal to other Gram-positive pathogenic bacteria, such as Staphylococcus aureus and Enterococcus faecalis. We show that PS900 can interact with PrfA and reduce the expression of virulence factors...
April 25, 2023: MBio
#31
JOURNAL ARTICLE
Audrey C Brown, Michelle D Warthan, Anush Aryal, Shiwei Liu, Jennifer L Guler
Mounting evidence demonstrates that nutritional environment can alter pathogen drug sensitivity. While the rich media used for in vitro culture contains supraphysiological nutrient concentrations, pathogens encounter a relatively restrictive environment in vivo . We assessed the effect of nutrient limitation on the protozoan parasite that causes malaria and demonstrated that short-term growth under physiologically relevant mild nutrient stress (or "metabolic priming") triggers increased tolerance of a potent antimalarial drug...
April 25, 2023: MBio
#32
JOURNAL ARTICLE
Emilia Wedel, Cristina Bernabe-Balas, Manuel Ares-Arroyo, Natalia Montero, Alfonso Santos-Lopez, Didier Mazel, Bruno Gonzalez-Zorn
Plasmids facilitate the vertical and horizontal spread of antimicrobial resistance genes between bacteria. The host range and adaptation of plasmids to new hosts determine their impact on the spread of resistance. In this work, we explore the mechanisms driving plasmid adaptation to novel hosts in experimental evolution. Using the small multicopy plasmid pB1000, usually found in Pasteurellaceae , we studied its adaptation to a host from a different bacterial family, Escherichia coli. We observed two different mechanisms of adaptation...
April 25, 2023: MBio
#33
LETTER
Peter Speck, Jason Mackenzie, Rowena A Bull, Barry Slobedman, Heidi Drummer, Johanna Fraser, Lara Herrero, Karla Helbig, Sarah Londrigan, Gregory Moseley, Natalie Prow, Grant Hansman, Robert Edwards, Chantelle Ahlenstiel, Allison Abendroth, David Tscharke, Jody Hobson-Peters, Robson Kriiger-Loterio, Rhys Parry, Glenn Marsh, Emma Harding, David A Jacques, Matthew J Gartner, Wen Shi Lee, Julie McAuley, Paola Vaz, Frank Sainsbury, Michelle D Tate, Jane Sinclair, Allison Imrie, Stephen Rawlinson, Andrew Harman, Jillian M Carr, Ebony A Monson, Merilyn Hibma, Timothy J Mahony, Thomas Tu, Robert J Center, Lok Bahadur Shrestha, Robyn Hall, Morgyn Warner, Vernon Ward, Danielle E Anderson, Nicholas S Eyre, Natalie E Netzler, Alison J Peel, Peter Revill, Michael Beard, Alistair R Legione, Alexandra J Spencer, Adi Idris, Jade Forwood, Subir Sarker, Damian F J Purcell, Nathan Bartlett, Joshua M Deerain, Bruce J Brew, Sassan Asgari, Helen Farrell, Alexander Khromykh, Daniel Enosi Tuipulotu, David Anderson, Sevim Mese, Yaman Tayyar, Kathryn Edenborough, Jasim Muhammad Uddin, Abrar Hussain, Connor J I Daymond, Jacinta Agius, Karyn N Johnson, Paniz Shirmast, Mahdi Abedinzadeshahri, Robin MacDiarmid, Caroline L Ashley, Jay Laws, Lucy L Furfaro, Thomas D Burton, Stephen M R Johnson, Zahra Telikani, Mary Petrone, Justin A Roby, Carolyn Samer, Andreas Suhrbier, April Van Der Kamp, Anthony Cunningham, Celeste Donato, Jackie Mahar, Wesley D Black, Subhash Vasudevan, Roman Lenchine, Kirsten Spann, Daniel J Rawle, Penny Rudd, Jessica Neil, Richard Kingston, Timothy P Newsome, Ki Wook Kim, Johnson Mak, Kym Lowry, Nathan Bryant, Joanne Meers, Jason A Roberts, Nigel McMillan, Larisa I Labzin, Andrii Slonchak, Leon E Hugo, Bennett Henzeler, Natalee D Newton, Cassandra T David, Patrick C Reading, Camille Esneau, Tatiana Briody, Najla Nasr, Donna McNeale, Brian McSharry, Omid Fakhri, Bethany A Horsburgh, Grant Logan, Paul Howley, Paul Young
No abstract text is available yet for this article.
April 25, 2023: MBio
#34
JOURNAL ARTICLE
Alexandra Hardy, Siddharth Bakshi, Wilhelm Furnon, Oscar MacLean, Quan Gu, Margus Varjak, Mariana Varela, Muhamad Afiq Aziz, Andrew E Shaw, Rute Maria Pinto, Natalia Cameron Ruiz, Catrina Mullan, Aislynn E Taggart, Ana Da Silva Filipe, Richard E Randall, Sam J Wilson, Meredith E Stewart, Massimo Palmarini
Infected hosts possess two alternative strategies to protect themselves against the negative impact of virus infections: resistance, used to abrogate virus replication, and disease tolerance, used to avoid tissue damage without controlling viral burden. The principles governing pathogen resistance are well understood, while less is known about those involved in disease tolerance. Here, we studied bluetongue virus (BTV), the cause of bluetongue disease of ruminants, as a model system to investigate the mechanisms of virus-host interactions correlating with disease tolerance...
April 25, 2023: MBio
#35
JOURNAL ARTICLE
Jovan Komluski, Michael Habig, Eva H Stukenbrock
Meiosis is associated with genetic changes in the genome-via recombination, gene conversion, and mutations. The occurrence of gene conversion and mutations during meiosis may further be influenced by the chromatin conformation, similar to the effect of the chromatin conformation on the mitotic mutation rate. To date, however, the exact distribution and type of meiosis-associated changes and the role of the chromatin conformation in this context are largely unexplored. Here, we determine recombination, gene conversion, and de novo mutations using whole-genome sequencing of all meiotic products of 23 individual meioses in Zymoseptoria tritici, an important pathogen of wheat...
April 24, 2023: MBio
#36
JOURNAL ARTICLE
Annie Yap, Ricarda Volz, Sanjoy Paul, W Scott Moye-Rowley, Hubertus Haas
Iron acquisition is crucial for virulence of the human pathogen Aspergillus fumigatus. Previous studies indicated that this mold regulates iron uptake via both siderophores and reductive iron assimilation by the GATA factor SreA and the SREBP regulator SrbA. Here, characterization of loss of function as well as hyperactive alleles revealed that transcriptional activation of iron uptake depends additionally on the Zn2 Cys6 regulator AtrR, most likely via cooperation with SrbA. Mutational analysis of the promoter of the iron permease-encoding ftrA gene identified a 210-bp sequence, which is both essential and sufficient to impart iron regulation...
April 24, 2023: MBio
#37
JOURNAL ARTICLE
David J Dubois, Sylia Chehade, Jean-Baptiste Marq, Kannan Venugopal, Bohumil Maco, Albert Tell I Puig, Dominique Soldati-Favre, Sabrina Marion
Members of the Apicomplexa phylum possess specialized secretory organelles that discharge, apically and in a timely regulated manner, key factors implicated in parasite motility, host cell invasion, egress and subversion of host cellular functions. The mechanisms regulating trafficking and apical docking of these secretory organelles are only partially elucidated. Here, we characterized two conserved endosomal trafficking regulators known to promote vesicle transport and/or fusion, HOOK and Fused Toes (FTS), in the context of organelle discharge in Toxoplasma gondii...
April 24, 2023: MBio
#38
JOURNAL ARTICLE
Zijian Guo, Yuanyuan He, Jian Xu, Ananya N Benegal, Steven L Brody, Michael D Vahey
Infection of individual cells by multiple virions plays critical roles in the replication and spread of many viruses, but mechanisms that control cellular coinfection during multicycle viral growth remain unclear. Here, we investigate virus-intrinsic factors that control cellular coinfection by influenza A virus (IAV). Using quantitative fluorescence to track the spread of virions from single infected cells, we identify the IAV surface protein neuraminidase (NA) as a key determinant of cellular coinfection...
April 20, 2023: MBio
#39
JOURNAL ARTICLE
David Bahry
A group of 156 virologists, including American Society of Microbiology journal editors-in-chief, has recently published across three ASM journals a "call for rational discourse" on such important topics as the origin of SARS-CoV-2 and gain of function research (e.g., F. Goodrum et al., mBio 14:e0018823, 2023, https://doi.org/10.1128/mbio.00188-23). Here, I answer the call, arguing that the origin of SARS-CoV-2 is unknown; that continued premature downplaying of a possible laboratory origin, now accompanied by a denial that this was ever so dismissed, undermines public trust in science; and that the benefits from risky gain-of-function research-of-concern are fewer than Goodrum et al...
April 19, 2023: MBio
#40
JOURNAL ARTICLE
Geun-Don Kim, Danye Qiu, Henning Jacob Jessen, Andreas Mayer
Cells stabilize intracellular inorganic phosphate (Pi ) to compromise between large biosynthetic needs and detrimental bioenergetic effects of Pi . Pi homeostasis in eukaryotes uses Syg1/Pho81/Xpr1 (SPX) domains, which are receptors for inositol pyrophosphates. We explored how polymerization and storage of Pi in acidocalcisome-like vacuoles supports Saccharomyces cerevisiae metabolism and how these cells recognize Pi scarcity. Whereas Pi starvation affects numerous metabolic pathways, beginning Pi scarcity affects few metabolites...
April 19, 2023: MBio
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